Full name : Broomfield Clarence A
First name : Clarence A
Mail : U.S. Army Medical Research Institute of Chemical Defense, 1917 Youngston Rd, Jarretsville, MD 21084 Aberdeen Proving Ground, MD 21010-5425
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Country : USA
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Comment
Clarence Broomfield was born on September 18, 1930 in Mount Morris, Michigan and passed away on November 2022. He was a major contributor to the field of AChE chemistry and the development of countermeasures against chemical warfare toxins. In particular he was the first to demonstrate that mutation of histidine could transform butyrylcholinesterase in a organophosphate hydrolase. He was an important mentor, a close collaborator, and a very good friend to many of the colleagues in the field
| Title : Comparison of human and guinea pig acetylcholinesterase sequences and rates of oxime-assisted reactivation - Cadieux_2010_Chem.Biol.Interact_187_229 |
| Author(s) : Cadieux CL , Broomfield CA , Kirkpatrick MG , Kazanski ME , Lenz DE , Cerasoli DM |
| Ref : Chemico-Biological Interactions , 187 :229 , 2010 |
| Abstract : |
| PubMedSearch : Cadieux_2010_Chem.Biol.Interact_187_229 |
| PubMedID: 20433814 |
| Gene_locus related to this paper: cavpo-d3ye96 |
| Title : Plant-derived human butyrylcholinesterase, but not an organophosphorous-compound hydrolyzing variant thereof, protects rodents against nerve agents - Geyer_2010_Proc.Natl.Acad.Sci.U.S.A_107_20251 |
| Author(s) : Geyer BC , Kannan L , Garnaud PE , Broomfield CA , Cadieux CL , Cherni I , Hodgins SM , Kasten SA , Kelley K , Kilbourne J , Oliver ZP , Otto TC , Puffenberger I , Reeves TE , Robbins N, 2nd , Woods RR , Soreq H , Lenz DE , Cerasoli DM , Mor TS |
| Ref : Proc Natl Acad Sci U S A , 107 :20251 , 2010 |
| Abstract : |
| PubMedSearch : Geyer_2010_Proc.Natl.Acad.Sci.U.S.A_107_20251 |
| PubMedID: 21059932 |
| Title : A collaborative endeavor to design cholinesterase-based catalytic scavengers against toxic organophosphorus esters - Masson_2008_Chem.Biol.Interact_175_273 |
| Author(s) : Masson P , Nachon F , Broomfield CA , Lenz DE , Verdier L , Schopfer LM , Lockridge O |
| Ref : Chemico-Biological Interactions , 175 :273 , 2008 |
| Abstract : |
| PubMedSearch : Masson_2008_Chem.Biol.Interact_175_273 |
| PubMedID: 18508040 |
| Title : Mutants of human butyrylcholinesterase with organophosphate hydrolase activity\; evidence that His117 is a general base catalyst for hydrolysis of echothiophate - Schopfer_2004_J.Med.Chem.Biol.Radiol.Def_2_1 |
| Author(s) : Schopfer LM , Boeck AT , Broomfield CA , Lockridge O |
| Ref : Journal of Medicinal Chemistryical Biology Radiol Def , 2 :1 , 2004 |
| Abstract : |
| PubMedSearch : Schopfer_2004_J.Med.Chem.Biol.Radiol.Def_2_1 |
| PubMedID: |
| Title : Evidence for nonacetylcholinesterase targets of organophosphorus nerve agent: supersensitivity of acetylcholinesterase knockout mouse to vx lethality - Duysen_2001_J.Pharmacol.Exp.Ther_299_528 |
| Author(s) : Duysen EG , Li B , Xie W , Schopfer LM , Anderson RS , Broomfield CA , Lockridge O |
| Ref : Journal of Pharmacology & Experimental Therapeutics , 299 :528 , 2001 |
| Abstract : |
| PubMedSearch : Duysen_2001_J.Pharmacol.Exp.Ther_299_528 |
| PubMedID: 11602663 |
| Title : Serum paraoxonase (PON1) isozymes: the quantitative analysis of isozymes affecting individual sensitivity to environmental chemicals - La_2001_Drug.Metab.Dispos_29_566 |
| Author(s) : La Du BN , Billecke S , Hsu C , Haley RW , Broomfield CA |
| Ref : Drug Metabolism & Disposition: The Biological Fate of Chemicals , 29 :566 , 2001 |
| Abstract : |
| PubMedSearch : La_2001_Drug.Metab.Dispos_29_566 |
| PubMedID: 11259353 |
| Title : Protective action of the serine protease inhibitor N-tosyl-L-lysine chloromethyl ketone (TLCK) against acute soman poisoning - Cowan_2001_J.Appl.Toxicol_21_293 |
| Author(s) : Cowan FM , Broomfield CA , Lenz DE , Shih TM |
| Ref : J Appl Toxicol , 21 :293 , 2001 |
| Abstract : |
| PubMedSearch : Cowan_2001_J.Appl.Toxicol_21_293 |
| PubMedID: 11481662 |
| Title : Expression of biologically active human butyrylcholinesterase in the cabbage looper (Trichoplusia ni) - Platteborze_2000_Biotechnol.Appl.Biochem_31 ( Pt 3)_225 |
| Author(s) : Platteborze PL , Broomfield CA |
| Ref : Biotechnol Appl Biochem , 31 ( Pt 3) :225 , 2000 |
| Abstract : |
| PubMedSearch : Platteborze_2000_Biotechnol.Appl.Biochem_31 ( Pt 3)_225 |
| PubMedID: 10814593 |
| Title : Characterization of a soluble mouse liver enzyme capable of hydrolyzing diisopropyl phosphorofluoridate - Billecke_1999_Chem.Biol.Interact_119-120_251 |
| Author(s) : Billecke SS , Primo-Parmo SL , Dunlop CS , Doorn JA , La Du BN , Broomfield CA |
| Ref : Chemico-Biological Interactions , 119-120 :251 , 1999 |
| Abstract : |
| PubMedSearch : Billecke_1999_Chem.Biol.Interact_119-120_251 |
| PubMedID: 10421459 |
| Title : Protein engineering of a human enzyme that hydrolyzes V and G nerve agents: design, construction and characterization - Broomfield_1999_Chem.Biol.Interact_119-120_413 |
| Author(s) : Broomfield CA , Lockridge O , Millard CB |
| Ref : Chemico-Biological Interactions , 119-120 :413 , 1999 |
| Abstract : |
| PubMedSearch : Broomfield_1999_Chem.Biol.Interact_119-120_413 |
| PubMedID: 10421478 |
| Title : Mechanical Aspects of the Phosphotriesterase Activity of Human Butyrylcholinesterase G117H Mutant - |
| Author(s) : Albaret C , Masson P , Broomfield CA , Kaim LE , Fortier PL |
| Ref : In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases , (Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp. :399 , 1998 |
| PubMedID: |
| Title : Poster: Mechanical Aspects of the Phosphotriesterase Activity of Human Butyrylcholinesterase G117H Mutant - |
| Author(s) : Albaret C , Masson P , Broomfield CA , Kaim LE , Fortier PL |
| Ref : In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases , (Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp. :434 , 1998 |
| PubMedID: |
| Title : Expression of Human Butyrylcholinesterase in Trichoplusia ni Insect Larvae - |
| Author(s) : Platteborze PL , Lockridge O , Broomfield CA |
| Ref : In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases , (Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp. :150 , 1998 |
| PubMedID: |
| Title : Reaction of Human Butyrylcholinesterase (BChE) H117 Enzymes with Carbamates - |
| Author(s) : Broomfield CA , Mills KV , Meier BM , Lockridge O , Millard CB |
| Ref : In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases , (Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp. :223 , 1998 |
| PubMedID: |
| Title : Organophosphorus acid anhydride hydrolase activity in human butyrylcholinesterase: synergy results in a somanase - Millard_1998_Biochemistry_37_237 |
| Author(s) : Millard CB , Lockridge O , Broomfield CA |
| Ref : Biochemistry , 37 :237 , 1998 |
| Abstract : |
| PubMedSearch : Millard_1998_Biochemistry_37_237 |
| PubMedID: 9425044 |
| Gene_locus related to this paper: human-BCHE |
| Title : Unique push-pull mechanism of dealkylation in soman-inhibited cholinesterases - Viragh_1997_Biochemistry_36_8243 |
| Author(s) : Viragh C , Akhmetshin R , Kovach IM , Broomfield CA |
| Ref : Biochemistry , 36 :8243 , 1997 |
| Abstract : |
| PubMedSearch : Viragh_1997_Biochemistry_36_8243 |
| PubMedID: 9204869 |
| Title : A single amino acid substitution, Gly117His, confers phosphotriesterase (organophosphorus acid anhydride hydrolase) activity on human butyrylcholinesterase - Lockridge_1997_Biochemistry_36_786 |
| Author(s) : Lockridge O , Blong RM , Masson P , Froment MT , Millard CB , Broomfield CA |
| Ref : Biochemistry , 36 :786 , 1997 |
| Abstract : |
| PubMedSearch : Lockridge_1997_Biochemistry_36_786 |
| PubMedID: 9020776 |
| Title : The effect of the human serum paraoxonase polymorphism is reversed with diazoxon, soman and sarin - Davies_1996_Nat.Genet_14_334 |
| Author(s) : Davies HG , Richter RJ , Keifer M , Broomfield CA , Sowalla J , Furlong CE |
| Ref : Nat Genet , 14 :334 , 1996 |
| Abstract : |
| PubMedSearch : Davies_1996_Nat.Genet_14_334 |
| PubMedID: 8896566 |
| Title : Anticholinesterases: medical applications of neurochemical principles. - Millard_1995_J.Neurochem_64_1909 |
| Author(s) : Millard CB , Broomfield CA |
| Ref : Journal of Neurochemistry , 64 :1909 , 1995 |
| Abstract : |
| PubMedSearch : Millard_1995_J.Neurochem_64_1909 |
| PubMedID: 7722478 |
| Title : Design and expression of organophosphorus acid anhydride hydrolase activity in human butyrylcholinesterase - Millard_1995_Biochemistry_34_15925 |
| Author(s) : Millard CB , Lockridge O , Broomfield CA |
| Ref : Biochemistry , 34 :15925 , 1995 |
| Abstract : |
| PubMedSearch : Millard_1995_Biochemistry_34_15925 |
| PubMedID: 8519749 |
| Title : Mutation of Human Butyrylcholinesterase Glycine 117 to Histidine Preserves Activity but Confers Resistance to Organophosphorus Inhibitors - |
| Author(s) : Broomfield CA , Millard CB , Lockridge O , Caviston TL |
| Ref : In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases , (Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp. :169 , 1995 |
| PubMedID: |
| Title : Behavioral decrements persist in rhesus monkeys trained on a serial probe recognition task despite protection against soman lethality by butyrylcholinesterase - Castro_1994_Neurotoxicol.Teratol_16_145 |
| Author(s) : Castro CA , Gresham VC , Finger AV , Maxwell DM , Solana RP , Lenz DE , Broomfield CA |
| Ref : Neurotoxicology & Teratology , 16 :145 , 1994 |
| Abstract : |
| PubMedSearch : Castro_1994_Neurotoxicol.Teratol_16_145 |
| PubMedID: 8052188 |
| Title : A purified recombinant organophosphorus acid anhydrase protects mice against soman - Broomfield_1993_Chem.Biol.Interact_87_279 |
| Author(s) : Broomfield CA |
| Ref : Chemico-Biological Interactions , 87 :279 , 1993 |
| Abstract : |
| PubMedSearch : Broomfield_1993_Chem.Biol.Interact_87_279 |
| PubMedID: 8393744 |
| Title : Catalytic antibodies hydrolysing organophosphorus esters - Brimfield_1993_Chem.Biol.Interact_87_95 |
| Author(s) : Brimfield AA , Lenz DE , Maxwell DM , Broomfield CA |
| Ref : Chemico-Biological Interactions , 87 :95 , 1993 |
| Abstract : |
| PubMedSearch : Brimfield_1993_Chem.Biol.Interact_87_95 |
| PubMedID: 8344006 |
| Title : Cholinesterase studies with (R) (+)- and (S)(-)-5-(1,3,3-trimethylindolinyl)-N-(1-phenylethyl)carbamate - Lieske_1992_J.Enzyme.Inhib_6_283 |
| Author(s) : Lieske CN , Gepp RT , Maxwell DM , Clark JH , Broomfield CA , Blumbergs P , Tseng CC |
| Ref : J Enzyme Inhib , 6 :283 , 1992 |
| Abstract : |
| PubMedSearch : Lieske_1992_J.Enzyme.Inhib_6_283 |
| PubMedID: 1284965 |
| Title : A computer model of glycosylated human butyrylcholinesterase - Millard_1992_Biochem.Biophys.Res.Commun_189_1280 |
| Author(s) : Millard CB , Broomfield CA |
| Ref : Biochemical & Biophysical Research Communications , 189 :1280 , 1992 |
| Abstract : |
| PubMedSearch : Millard_1992_Biochem.Biophys.Res.Commun_189_1280 |
| PubMedID: 1482344 |
| Title : Protection by butyrylcholinesterase against organophosphorus poisoning in nonhuman primates - Broomfield_1991_J.Pharmacol.Exp.Ther_259_633 |
| Author(s) : Broomfield CA , Maxwell DM , Solana RP , Castro CA , Finger AV , Lenz DE |
| Ref : Journal of Pharmacology & Experimental Therapeutics , 259 :633 , 1991 |
| Abstract : |
| PubMedSearch : Broomfield_1991_J.Pharmacol.Exp.Ther_259_633 |
| PubMedID: 1941611 |
| Title : A novel tertiary pyridostigmine derivative [3-(N,N-dimethylcarbamyloxy)-1-methyl-delta 3-tetrahydropyridine]: anticholinesterase properties and efficacy against soman - Ray_1991_Fundam.Appl.Toxicol_16_267 |
| Author(s) : Ray R , Clark OEd , Ford KW , Knight KR , Harris LW , Broomfield CA |
| Ref : Fundamental & Applied Toxicology , 16 :267 , 1991 |
| Abstract : |
| PubMedSearch : Ray_1991_Fundam.Appl.Toxicol_16_267 |
| PubMedID: 2055358 |
| Title : Poster: Hydrolysis of nerve agents by plasma enzymes - |
| Author(s) : Broomfield CA , Ford KW |
| Ref : In: Cholinesterases: Structure, Function, Mechanism, Genetics, and Cell Biology , (Massoulie J, Barnard EA, Chatonnet A, Bacou F, Doctor BP, Quinn DM) American Chemical Society, Washington, DC :307 , 1991 |
| PubMedID: |
| Title : Poster: Protection by butyry1cholinesterase against soman poisoning - |
| Author(s) : Lenz DE , Broomfield CA , Maxwell CA , Solana RP , Finger AV , Woodard CL |
| Ref : In: Cholinesterases: Structure, Function, Mechanism, Genetics, and Cell Biology , (Massoulie J, Barnard EA, Chatonnet A, Bacou F, Doctor BP, Quinn DM) American Chemical Society, Washington, DC :302 , 1991 |
| PubMedID: |
| Title : pH effects in the spontaneous reactivation of phosphinylated acetylcholinesterase - Lieske_1990_Life.Sci_46_1189 |
| Author(s) : Lieske CN , Gessner CE , Gepp RT , Clark JH , Meyer HG , Broomfield CA |
| Ref : Life Sciences , 46 :1189 , 1990 |
| Abstract : |
| PubMedSearch : Lieske_1990_Life.Sci_46_1189 |
| PubMedID: 2338884 |
| Title : Binding of soman antidotes to acetylcholine receptors - Broomfield_1987_Biochem.Pharmacol_36_1017 |
| Author(s) : Broomfield CA , Dembure IJ , Cuculis J |
| Ref : Biochemical Pharmacology , 36 :1017 , 1987 |
| Abstract : |
| PubMedSearch : Broomfield_1987_Biochem.Pharmacol_36_1017 |
| PubMedID: 3566797 |
| Title : Poster: Use of methyl-quinuclidinyl benzilate for the assay of muscarinic cholinergic binding sites - |
| Author(s) : Filbert MG , Broomfield CA , Dembure IJ , Baskin SI |
| Ref : Trends in Pharmacological Sciences , Suppl :79 , 1986 |
| PubMedID: |
| Title : Poster: The binding of soman antidotes to muscarinic acetylcholine receptors - |
| Author(s) : Broomfield CA , Dembure IJ , Cuculis J |
| Ref : Trends in Pharmacological Sciences , Suppl :81 , 1986 |
| PubMedID: |
| Title : The effects of atropine-oxime therapy on cholinesterase activity and survival of animals intoxicated with p-nitrophenyl di-n-butylphosphinate - |
| Author(s) : Harris LW , Stitcher DL , Heyl WC , Lieske CN , Lowe JR , Clark JH , Broomfield CA |
| Ref : Toxicology & Applied Pharmacology , 49 :23 , 1979 |
| PubMedID: 473199 |
| Title : Effects of 1,1'-oxydimethylene bis-(4-tert-butylpyridinium chloride) (SAD-128) and decamethonium on reactivation of soman- and sarin-inhibited cholinesterase by oximes - |
| Author(s) : Harris LW , Heyl WC , Stitcher DL , Broomfield CA |
| Ref : Biochemical Pharmacology , 27 :757 , 1978 |
| PubMedID: 656114 |
| Title : Spontaneous reactivation of acetylcholinesterase following organophosphate inhibition. I. An analysis of anomalous reactivation kinetics - Hovanec_1977_Biochim.Biophys.Acta_483_312 |
| Author(s) : Hovanec JW , Broomfield CA , Steinberg GM , Lanks KW , Lieske CN |
| Ref : Biochimica & Biophysica Acta , 483 :312 , 1977 |
| Abstract : |
| PubMedSearch : Hovanec_1977_Biochim.Biophys.Acta_483_312 |
| PubMedID: 19068 |
| Title : Guinea pig brain acetylcholinesterase: partial purification by affinity chromatography - |
| Author(s) : Yamamura HI , Reichard DW , Gardner TL , Morrisett JD , Broomfield CA |
| Ref : Biochimica & Biophysica Acta , 302 :305 , 1973 |
| PubMedID: 4699242 |
| Title : A comparative study of spin-labeled serine enzymes: acetylcholinesterase, trypsin, -chymotrypsin, elastase, and subtilisin - |
| Author(s) : Morrisett JD , Broomfield CA |
| Ref : Journal of Biological Chemistry , 247 :7224 , 1972 |
| PubMedID: 4344643 |
| Title : A new spin label specific for the active site of serine enzymes - |
| Author(s) : Morrisett JD , Broomfield CA , Hackley BE, Jr. |
| Ref : Journal of Biological Chemistry , 244 :5758 , 1969 |
| PubMedID: 4310606 |