Broomfield CA

General

Full name : Broomfield Clarence A

First name : Clarence A

Mail : U.S. Army Medical Research Institute of Chemical Defense, 1917 Youngston Rd, Jarretsville, MD 21084 Aberdeen Proving Ground, MD 21010-5425

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Country : USA

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Comment
Clarence Broomfield was born on September 18, 1930 in Mount Morris, Michigan and passed away on November 2022. He was a major contributor to the field of AChE chemistry and the development of countermeasures against chemical warfare toxins. In particular he was the first to demonstrate that mutation of histidine could transform butyrylcholinesterase in a organophosphate hydrolase. He was an important mentor, a close collaborator, and a very good friend to many of the colleagues in the field

References (40)

Title : Comparison of human and guinea pig acetylcholinesterase sequences and rates of oxime-assisted reactivation - Cadieux_2010_Chem.Biol.Interact_187_229
Author(s) : Cadieux CL , Broomfield CA , Kirkpatrick MG , Kazanski ME , Lenz DE , Cerasoli DM
Ref : Chemico-Biological Interactions , 187 :229 , 2010
Abstract :
PubMedSearch : Cadieux_2010_Chem.Biol.Interact_187_229
PubMedID: 20433814
Gene_locus related to this paper: cavpo-d3ye96

Title : Plant-derived human butyrylcholinesterase, but not an organophosphorous-compound hydrolyzing variant thereof, protects rodents against nerve agents - Geyer_2010_Proc.Natl.Acad.Sci.U.S.A_107_20251
Author(s) : Geyer BC , Kannan L , Garnaud PE , Broomfield CA , Cadieux CL , Cherni I , Hodgins SM , Kasten SA , Kelley K , Kilbourne J , Oliver ZP , Otto TC , Puffenberger I , Reeves TE , Robbins N, 2nd , Woods RR , Soreq H , Lenz DE , Cerasoli DM , Mor TS
Ref : Proc Natl Acad Sci U S A , 107 :20251 , 2010
Abstract :
PubMedSearch : Geyer_2010_Proc.Natl.Acad.Sci.U.S.A_107_20251
PubMedID: 21059932

Title : A collaborative endeavor to design cholinesterase-based catalytic scavengers against toxic organophosphorus esters - Masson_2008_Chem.Biol.Interact_175_273
Author(s) : Masson P , Nachon F , Broomfield CA , Lenz DE , Verdier L , Schopfer LM , Lockridge O
Ref : Chemico-Biological Interactions , 175 :273 , 2008
Abstract :
PubMedSearch : Masson_2008_Chem.Biol.Interact_175_273
PubMedID: 18508040

Title : Mutants of human butyrylcholinesterase with organophosphate hydrolase activity\; evidence that His117 is a general base catalyst for hydrolysis of echothiophate - Schopfer_2004_J.Med.Chem.Biol.Radiol.Def_2_1
Author(s) : Schopfer LM , Boeck AT , Broomfield CA , Lockridge O
Ref : Journal of Medicinal Chemistryical Biology Radiol Def , 2 :1 , 2004
Abstract :
PubMedSearch : Schopfer_2004_J.Med.Chem.Biol.Radiol.Def_2_1
PubMedID:

Title : Evidence for nonacetylcholinesterase targets of organophosphorus nerve agent: supersensitivity of acetylcholinesterase knockout mouse to vx lethality - Duysen_2001_J.Pharmacol.Exp.Ther_299_528
Author(s) : Duysen EG , Li B , Xie W , Schopfer LM , Anderson RS , Broomfield CA , Lockridge O
Ref : Journal of Pharmacology & Experimental Therapeutics , 299 :528 , 2001
Abstract :
PubMedSearch : Duysen_2001_J.Pharmacol.Exp.Ther_299_528
PubMedID: 11602663

Title : Serum paraoxonase (PON1) isozymes: the quantitative analysis of isozymes affecting individual sensitivity to environmental chemicals - La_2001_Drug.Metab.Dispos_29_566
Author(s) : La Du BN , Billecke S , Hsu C , Haley RW , Broomfield CA
Ref : Drug Metabolism & Disposition: The Biological Fate of Chemicals , 29 :566 , 2001
Abstract :
PubMedSearch : La_2001_Drug.Metab.Dispos_29_566
PubMedID: 11259353

Title : Protective action of the serine protease inhibitor N-tosyl-L-lysine chloromethyl ketone (TLCK) against acute soman poisoning - Cowan_2001_J.Appl.Toxicol_21_293
Author(s) : Cowan FM , Broomfield CA , Lenz DE , Shih TM
Ref : J Appl Toxicol , 21 :293 , 2001
Abstract :
PubMedSearch : Cowan_2001_J.Appl.Toxicol_21_293
PubMedID: 11481662

Title : Expression of biologically active human butyrylcholinesterase in the cabbage looper (Trichoplusia ni) - Platteborze_2000_Biotechnol.Appl.Biochem_31 ( Pt 3)_225
Author(s) : Platteborze PL , Broomfield CA
Ref : Biotechnol Appl Biochem , 31 ( Pt 3) :225 , 2000
Abstract :
PubMedSearch : Platteborze_2000_Biotechnol.Appl.Biochem_31 ( Pt 3)_225
PubMedID: 10814593

Title : Characterization of a soluble mouse liver enzyme capable of hydrolyzing diisopropyl phosphorofluoridate - Billecke_1999_Chem.Biol.Interact_119-120_251
Author(s) : Billecke SS , Primo-Parmo SL , Dunlop CS , Doorn JA , La Du BN , Broomfield CA
Ref : Chemico-Biological Interactions , 119-120 :251 , 1999
Abstract :
PubMedSearch : Billecke_1999_Chem.Biol.Interact_119-120_251
PubMedID: 10421459

Title : Protein engineering of a human enzyme that hydrolyzes V and G nerve agents: design, construction and characterization - Broomfield_1999_Chem.Biol.Interact_119-120_413
Author(s) : Broomfield CA , Lockridge O , Millard CB
Ref : Chemico-Biological Interactions , 119-120 :413 , 1999
Abstract :
PubMedSearch : Broomfield_1999_Chem.Biol.Interact_119-120_413
PubMedID: 10421478

Title : Organophosphorus acid anhydride hydrolase activity in human butyrylcholinesterase: synergy results in a somanase - Millard_1998_Biochemistry_37_237
Author(s) : Millard CB , Lockridge O , Broomfield CA
Ref : Biochemistry , 37 :237 , 1998
Abstract :
PubMedSearch : Millard_1998_Biochemistry_37_237
PubMedID: 9425044
Gene_locus related to this paper: human-BCHE

Title : Mechanical Aspects of the Phosphotriesterase Activity of Human Butyrylcholinesterase G117H Mutant -
Author(s) : Albaret C , Masson P , Broomfield CA , Kaim LE , Fortier PL
Ref : In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases , (Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp. :399 , 1998
PubMedID:

Title : Poster: Mechanical Aspects of the Phosphotriesterase Activity of Human Butyrylcholinesterase G117H Mutant -
Author(s) : Albaret C , Masson P , Broomfield CA , Kaim LE , Fortier PL
Ref : In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases , (Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp. :434 , 1998
PubMedID:

Title : Expression of Human Butyrylcholinesterase in Trichoplusia ni Insect Larvae -
Author(s) : Platteborze PL , Lockridge O , Broomfield CA
Ref : In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases , (Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp. :150 , 1998
PubMedID:

Title : Reaction of Human Butyrylcholinesterase (BChE) H117 Enzymes with Carbamates -
Author(s) : Broomfield CA , Mills KV , Meier BM , Lockridge O , Millard CB
Ref : In: Structure and Function of Cholinesterases and Related Proteins - Proceedings of Sixth International Meeting on Cholinesterases , (Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. Eds) Plenum Publishing Corp. :223 , 1998
PubMedID:

Title : A single amino acid substitution, Gly117His, confers phosphotriesterase (organophosphorus acid anhydride hydrolase) activity on human butyrylcholinesterase - Lockridge_1997_Biochemistry_36_786
Author(s) : Lockridge O , Blong RM , Masson P , Froment MT , Millard CB , Broomfield CA
Ref : Biochemistry , 36 :786 , 1997
Abstract :
PubMedSearch : Lockridge_1997_Biochemistry_36_786
PubMedID: 9020776

Title : Unique push-pull mechanism of dealkylation in soman-inhibited cholinesterases - Viragh_1997_Biochemistry_36_8243
Author(s) : Viragh C , Akhmetshin R , Kovach IM , Broomfield CA
Ref : Biochemistry , 36 :8243 , 1997
Abstract :
PubMedSearch : Viragh_1997_Biochemistry_36_8243
PubMedID: 9204869

Title : The effect of the human serum paraoxonase polymorphism is reversed with diazoxon, soman and sarin - Davies_1996_Nat.Genet_14_334
Author(s) : Davies HG , Richter RJ , Keifer M , Broomfield CA , Sowalla J , Furlong CE
Ref : Nat Genet , 14 :334 , 1996
Abstract :
PubMedSearch : Davies_1996_Nat.Genet_14_334
PubMedID: 8896566

Title : Anticholinesterases: medical applications of neurochemical principles. - Millard_1995_J.Neurochem_64_1909
Author(s) : Millard CB , Broomfield CA
Ref : Journal of Neurochemistry , 64 :1909 , 1995
Abstract :
PubMedSearch : Millard_1995_J.Neurochem_64_1909
PubMedID: 7722478

Title : Mutation of Human Butyrylcholinesterase Glycine 117 to Histidine Preserves Activity but Confers Resistance to Organophosphorus Inhibitors -
Author(s) : Broomfield CA , Millard CB , Lockridge O , Caviston TL
Ref : In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases , (Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp. :169 , 1995
PubMedID:

Title : Design and expression of organophosphorus acid anhydride hydrolase activity in human butyrylcholinesterase - Millard_1995_Biochemistry_34_15925
Author(s) : Millard CB , Lockridge O , Broomfield CA
Ref : Biochemistry , 34 :15925 , 1995
Abstract :
PubMedSearch : Millard_1995_Biochemistry_34_15925
PubMedID: 8519749

Title : Behavioral decrements persist in rhesus monkeys trained on a serial probe recognition task despite protection against soman lethality by butyrylcholinesterase - Castro_1994_Neurotoxicol.Teratol_16_145
Author(s) : Castro CA , Gresham VC , Finger AV , Maxwell DM , Solana RP , Lenz DE , Broomfield CA
Ref : Neurotoxicology & Teratology , 16 :145 , 1994
Abstract :
PubMedSearch : Castro_1994_Neurotoxicol.Teratol_16_145
PubMedID: 8052188

Title : A purified recombinant organophosphorus acid anhydrase protects mice against soman - Broomfield_1993_Chem.Biol.Interact_87_279
Author(s) : Broomfield CA
Ref : Chemico-Biological Interactions , 87 :279 , 1993
Abstract :
PubMedSearch : Broomfield_1993_Chem.Biol.Interact_87_279
PubMedID: 8393744

Title : Catalytic antibodies hydrolysing organophosphorus esters - Brimfield_1993_Chem.Biol.Interact_87_95
Author(s) : Brimfield AA , Lenz DE , Maxwell DM , Broomfield CA
Ref : Chemico-Biological Interactions , 87 :95 , 1993
Abstract :
PubMedSearch : Brimfield_1993_Chem.Biol.Interact_87_95
PubMedID: 8344006

Title : A computer model of glycosylated human butyrylcholinesterase - Millard_1992_Biochem.Biophys.Res.Commun_189_1280
Author(s) : Millard CB , Broomfield CA
Ref : Biochemical & Biophysical Research Communications , 189 :1280 , 1992
Abstract :
PubMedSearch : Millard_1992_Biochem.Biophys.Res.Commun_189_1280
PubMedID: 1482344

Title : Cholinesterase studies with (R) (+)- and (S)(-)-5-(1,3,3-trimethylindolinyl)-N-(1-phenylethyl)carbamate - Lieske_1992_J.Enzyme.Inhib_6_283
Author(s) : Lieske CN , Gepp RT , Maxwell DM , Clark JH , Broomfield CA , Blumbergs P , Tseng CC
Ref : J Enzyme Inhib , 6 :283 , 1992
Abstract :
PubMedSearch : Lieske_1992_J.Enzyme.Inhib_6_283
PubMedID: 1284965

Title : A novel tertiary pyridostigmine derivative [3-(N,N-dimethylcarbamyloxy)-1-methyl-delta 3-tetrahydropyridine]: anticholinesterase properties and efficacy against soman - Ray_1991_Fundam.Appl.Toxicol_16_267
Author(s) : Ray R , Clark OEd , Ford KW , Knight KR , Harris LW , Broomfield CA
Ref : Fundamental & Applied Toxicology , 16 :267 , 1991
Abstract :
PubMedSearch : Ray_1991_Fundam.Appl.Toxicol_16_267
PubMedID: 2055358

Title : Poster: Hydrolysis of nerve agents by plasma enzymes -
Author(s) : Broomfield CA , Ford KW
Ref : In: Cholinesterases: Structure, Function, Mechanism, Genetics, and Cell Biology , (Massoulie J, Barnard EA, Chatonnet A, Bacou F, Doctor BP, Quinn DM) American Chemical Society, Washington, DC :307 , 1991
PubMedID:

Title : Protection by butyrylcholinesterase against organophosphorus poisoning in nonhuman primates - Broomfield_1991_J.Pharmacol.Exp.Ther_259_633
Author(s) : Broomfield CA , Maxwell DM , Solana RP , Castro CA , Finger AV , Lenz DE
Ref : Journal of Pharmacology & Experimental Therapeutics , 259 :633 , 1991
Abstract :
PubMedSearch : Broomfield_1991_J.Pharmacol.Exp.Ther_259_633
PubMedID: 1941611

Title : Poster: Protection by butyry1cholinesterase against soman poisoning -
Author(s) : Lenz DE , Broomfield CA , Maxwell CA , Solana RP , Finger AV , Woodard CL
Ref : In: Cholinesterases: Structure, Function, Mechanism, Genetics, and Cell Biology , (Massoulie J, Barnard EA, Chatonnet A, Bacou F, Doctor BP, Quinn DM) American Chemical Society, Washington, DC :302 , 1991
PubMedID:

Title : pH effects in the spontaneous reactivation of phosphinylated acetylcholinesterase - Lieske_1990_Life.Sci_46_1189
Author(s) : Lieske CN , Gessner CE , Gepp RT , Clark JH , Meyer HG , Broomfield CA
Ref : Life Sciences , 46 :1189 , 1990
Abstract :
PubMedSearch : Lieske_1990_Life.Sci_46_1189
PubMedID: 2338884

Title : Binding of soman antidotes to acetylcholine receptors - Broomfield_1987_Biochem.Pharmacol_36_1017
Author(s) : Broomfield CA , Dembure IJ , Cuculis J
Ref : Biochemical Pharmacology , 36 :1017 , 1987
Abstract :
PubMedSearch : Broomfield_1987_Biochem.Pharmacol_36_1017
PubMedID: 3566797

Title : Poster: Use of methyl-quinuclidinyl benzilate for the assay of muscarinic cholinergic binding sites -
Author(s) : Filbert MG , Broomfield CA , Dembure IJ , Baskin SI
Ref : Trends in Pharmacological Sciences , Suppl :79 , 1986
PubMedID:

Title : Poster: The binding of soman antidotes to muscarinic acetylcholine receptors -
Author(s) : Broomfield CA , Dembure IJ , Cuculis J
Ref : Trends in Pharmacological Sciences , Suppl :81 , 1986
PubMedID:

Title : The effects of atropine-oxime therapy on cholinesterase activity and survival of animals intoxicated with p-nitrophenyl di-n-butylphosphinate -
Author(s) : Harris LW , Stitcher DL , Heyl WC , Lieske CN , Lowe JR , Clark JH , Broomfield CA
Ref : Toxicology & Applied Pharmacology , 49 :23 , 1979
PubMedID: 473199

Title : Effects of 1,1'-oxydimethylene bis-(4-tert-butylpyridinium chloride) (SAD-128) and decamethonium on reactivation of soman- and sarin-inhibited cholinesterase by oximes -
Author(s) : Harris LW , Heyl WC , Stitcher DL , Broomfield CA
Ref : Biochemical Pharmacology , 27 :757 , 1978
PubMedID: 656114

Title : Spontaneous reactivation of acetylcholinesterase following organophosphate inhibition. I. An analysis of anomalous reactivation kinetics - Hovanec_1977_Biochim.Biophys.Acta_483_312
Author(s) : Hovanec JW , Broomfield CA , Steinberg GM , Lanks KW , Lieske CN
Ref : Biochimica & Biophysica Acta , 483 :312 , 1977
Abstract :
PubMedSearch : Hovanec_1977_Biochim.Biophys.Acta_483_312
PubMedID: 19068

Title : Guinea pig brain acetylcholinesterase: partial purification by affinity chromatography -
Author(s) : Yamamura HI , Reichard DW , Gardner TL , Morrisett JD , Broomfield CA
Ref : Biochimica & Biophysica Acta , 302 :305 , 1973
PubMedID: 4699242

Title : A comparative study of spin-labeled serine enzymes: acetylcholinesterase, trypsin, -chymotrypsin, elastase, and subtilisin -
Author(s) : Morrisett JD , Broomfield CA
Ref : Journal of Biological Chemistry , 247 :7224 , 1972
PubMedID: 4344643

Title : A new spin label specific for the active site of serine enzymes -
Author(s) : Morrisett JD , Broomfield CA , Hackley BE, Jr.
Ref : Journal of Biological Chemistry , 244 :5758 , 1969
PubMedID: 4310606