W86E_human-ACHE

General

Gene Locus : human-ACHE

Mode of mutation : Site directed mutagenesis

Disease :

Summary : Choline binding site cation-aromatic interaction, 660 fold decrease affinity for ACTh no effect on uncharged substrate Ordentlich_1995_J.Biol.Chem_270_2082 Shafferman_1995_5th.ChE.Meeting.Madras__189 || Signal transduction Y133 maintains the correct orientation of W86 Ordentlich_1995_J.Biol.Chem_270_2082 Shafferman_1995_5th.ChE.Meeting.Madras__189

AAA Change :

Allelic Variant :

Risk Factor :

Inhibitor :

Structure :

Disease by interaction :

Interact Gene Locus :

Xenobiotic sensitivity :

Modification : Choline binding site || Signal transduction

Torpedo_number : 84

Kinetic Parameter : Acetylthiocholine_W86E_human-ACHE, 3,3-dimethylbutylthioacetate_W86E_human-ACHE, Edrophonium_W86E_human-ACHE, Propidium_W86E_human-ACHE, Decamethonium_W86E_human-ACHE

News : No news

Comment :
p.W86E Trp86Glu (p.W117E Trp117Glu in primary sequence with 31 amino-acids signal peptide) Choline binding site\;cation-aromatic interaction, 660 fold decrease affinity for ACTh no effect on uncharged substrate\; Y133 maintains the correct orientation of W86

References (3)

Title : Molecular Aspects of Catalysis and of Allosteric Regulation of Aceytlcholinesterases -
Author(s) : Shafferman A , Ordentlich A , Barak D , Kronman C , Ariel N , Leitner M , Segall Y , Bromberg A , Reuveny S , Marcus D , Bino T , Lazar A , Cohen S , Velan B
Ref : In Enzyme of the Cholinesterase Family - Proceedings of Fifth International Meeting on Cholinesterases , (Quinn, D.M., Balasubramanian, A.S., Doctor, B.P., Taylor, P., Eds) Plenum Publishing Corp. :189 , 1995
PubMedID:

Title : Contribution of aromatic moieties of tyrosine 133 and of the anionic subsite tryptophan 86 to catalytic efficiency and allosteric modulation of acetylcholinesterase - Ordentlich_1995_J.Biol.Chem_270_2082
Author(s) : Ordentlich A , Barak D , Kronman C , Ariel N , Segall Y , Velan B , Shafferman A
Ref : Journal of Biological Chemistry , 270 :2082 , 1995
PubMedID: 7836436

Title : Substrate inhibition of acetylcholinesterase: residues affecting signal transduction from the surface to the catalytic center - Shafferman_1992_EMBO.J_11_3561
Author(s) : Shafferman A , Velan B , Ordentlich A , Kronman C , Grosfeld H , Leitner M , Flashner Y , Cohen S , Barak D , Ariel N
Ref : EMBO Journal , 11 :3561 , 1992
PubMedID: 1396557