Amitai_1998_FEBS.Lett_438_195

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Title : Oxidative biodegradation of phosphorothiolates by fungal laccase - Amitai_1998_FEBS.Lett_438_195
Author(s) : Amitai G , Adani R , Sod-Moriah G , Rabinovitz I , Vincze A , Leader H , Chefetz B , Leibovitz-Persky L , Friesem D , Hadar Y
Ref : FEBS Letters , 438 :195 , 1998
Abstract : Organophosphorus (OP) insecticides and nerve agents that contain P-S bond are relatively more resistant to enzymatic hydrolysis. Purified phenol oxidase (laccase) from the white rot fungus Pleurotus ostreatus (Po) together with the mediator 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) displayed complete and rapid oxidative degradation of the nerve agents VX and Russian VX (RVX) and the insecticide analog diisopropyl-Amiton with specific activity: k(sp) = 2200, 667 and 1833 nmol min(-1) mg(-1), respectively (pH 7.4, 37 degrees C). A molar ratio of 1:20 for OP/ABTS and 0.05 M phosphate at pH 7.4 provided the highest degradation rate of VX and RVX. The thermostable laccase purified from the fungus Chaetomium thermophilium (Ct) in the presence of ABTS caused a 52-fold slower degradation of VX with k(sp) = 42 nmol min(-1) mg(-1). The enzymatic biodegradation products were identified by 31P-NMR and GC/MS analysis.
ESTHER : Amitai_1998_FEBS.Lett_438_195
PubMedSearch : Amitai_1998_FEBS.Lett_438_195
PubMedID: 9827544

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Amitai G, Adani R, Sod-Moriah G, Rabinovitz I, Vincze A, Leader H, Chefetz B, Leibovitz-Persky L, Friesem D, Hadar Y (1998)
Oxidative biodegradation of phosphorothiolates by fungal laccase
FEBS Letters 438 :195

Amitai G, Adani R, Sod-Moriah G, Rabinovitz I, Vincze A, Leader H, Chefetz B, Leibovitz-Persky L, Friesem D, Hadar Y (1998)
FEBS Letters 438 :195