Gartemann_2008_J.Bacteriol_190_2138

Reference

Title : The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity - Gartemann_2008_J.Bacteriol_190_2138
Author(s) : Gartemann KH , Abt B , Bekel T , Burger A , Engemann J , Flugel M , Gaigalat L , Goesmann A , Grafen I , Kalinowski J , Kaup O , Kirchner O , Krause L , Linke B , McHardy A , Meyer F , Pohle S , Ruckert C , Schneiker S , Zellermann EM , Puhler A , Eichenlaub R , Kaiser O , Bartels D
Ref : Journal of Bacteriology , 190 :2138 , 2008
Abstract :

Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete that causes bacterial wilt and canker of tomato. The nucleotide sequence of the genome of strain NCPPB382 was determined. The chromosome is circular, consists of 3.298 Mb, and has a high G+C content (72.6%). Annotation revealed 3,080 putative protein-encoding sequences; only 26 pseudogenes were detected. Two rrn operons, 45 tRNAs, and three small stable RNA genes were found. The two circular plasmids, pCM1 (27.4 kbp) and pCM2 (70.0 kbp), which carry pathogenicity genes and thus are essential for virulence, have lower G+C contents (66.5 and 67.6%, respectively). In contrast to the genome of the closely related organism Clavibacter michiganensis subsp. sepedonicus, the genome of C. michiganensis subsp. michiganensis lacks complete insertion elements and transposons. The 129-kb chp/tomA region with a low G+C content near the chromosomal origin of replication was shown to be necessary for pathogenicity. This region contains numerous genes encoding proteins involved in uptake and metabolism of sugars and several serine proteases. There is evidence that single genes located in this region, especially genes encoding serine proteases, are required for efficient colonization of the host. Although C. michiganensis subsp. michiganensis grows mainly in the xylem of tomato plants, no evidence for pronounced genome reduction was found. C. michiganensis subsp. michiganensis seems to have as many transporters and regulators as typical soil-inhabiting bacteria. However, the apparent lack of a sulfate reduction pathway, which makes C. michiganensis subsp. michiganensis dependent on reduced sulfur compounds for growth, is probably the reason for the poor survival of C. michiganensis subsp. michiganensis in soil.

PubMedSearch : Gartemann_2008_J.Bacteriol_190_2138
PubMedID: 18192381
Gene_locus related to this paper: clam3-a5cln9 , clam3-a5clw2 , clam3-a5cm48 , clam3-a5cmg3 , clam3-a5cmk0 , clam3-a5cn86 , clam3-a5cr52 , clam3-a5cr57 , clam3-a5crq6 , clam3-a5csc5 , clam3-metx , clams-b0rcr0 , clams-b0rdd6 , clams-b0rde2 , clams-b0rga8 , clams-b0riv5 , clam3-a5cui5 , clam3-a5cp43 , clam3-a5cp56

Related information

Gene_locus clam3-a5cln9    clam3-a5clw2    clam3-a5cm48    clam3-a5cmg3    clam3-a5cmk0    clam3-a5cn86    clam3-a5cr52    clam3-a5cr57    clam3-a5crq6    clam3-a5csc5    clam3-metx    clams-b0rcr0    clams-b0rdd6    clams-b0rde2    clams-b0rga8    clams-b0riv5    clam3-a5cui5    clam3-a5cp43    clam3-a5cp56

Citations formats

Gartemann KH, Abt B, Bekel T, Burger A, Engemann J, Flugel M, Gaigalat L, Goesmann A, Grafen I, Kalinowski J, Kaup O, Kirchner O, Krause L, Linke B, McHardy A, Meyer F, Pohle S, Ruckert C, Schneiker S, Zellermann EM, Puhler A, Eichenlaub R, Kaiser O, Bartels D (2008)
The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity
Journal of Bacteriology 190 :2138

Gartemann KH, Abt B, Bekel T, Burger A, Engemann J, Flugel M, Gaigalat L, Goesmann A, Grafen I, Kalinowski J, Kaup O, Kirchner O, Krause L, Linke B, McHardy A, Meyer F, Pohle S, Ruckert C, Schneiker S, Zellermann EM, Puhler A, Eichenlaub R, Kaiser O, Bartels D (2008)
Journal of Bacteriology 190 :2138