Kaiser O

References (11)

Title : Safety and efficacy of mTOR inhibitor treatment in patients with tuberous sclerosis complex under 2 years of age - a multicenter retrospective study - Saffari_2019_Orphanet.J.Rare.Dis_14_96
Author(s) : Saffari A , Brosse I , Wiemer-Kruel A , Wilken B , Kreuzaler P , Hahn A , Bernhard MK , van Tilburg CM , Hoffmann GF , Gorenflo M , Hethey S , Kaiser O , Kolker S , Wagner R , Witt O , Merkenschlager A , Mockel A , Roser T , Schlump JU , Serfling A , Spiegler J , Milde T , Ziegler A , Syrbe S
Ref : Orphanet J Rare Dis , 14 :96 , 2019
Abstract : BACKGROUND: Tuberous sclerosis complex (TSC) is a multisystem disease with prominent neurologic manifestations such as epilepsy, cognitive impairment and autism spectrum disorder. mTOR inhibitors have successfully been used to treat TSC-related manifestations in older children and adults. However, data on their safety and efficacy in infants and young children are scarce. The objective of this study is to assess the utility and safety of mTOR inhibitor treatment in TSC patients under the age of 2 years. RESULTS: A total of 17 children (median age at study inclusion 2.4 years, range 0-6; 12 males, 5 females) with TSC who received early mTOR inhibitor therapy were studied. mTOR inhibitor treatment was started at a median age of 5 months (range 0-19 months). Reasons for initiation of treatment were cardiac rhabdomyomas (6 cases), subependymal giant cell astrocytomas (SEGA, 5 cases), combination of cardiac rhabdomyomas and SEGA (1 case), refractory epilepsy (4 cases) and disabling congenital focal lymphedema (1 case). In all cases everolimus was used. Everolimus therapy was overall well tolerated. Adverse events were classified according to the Common Terminology Criteria of Adverse Events (CTCAE, Version 5.0). Grade 1-2 adverse events occurred in 12 patients and included mild transient stomatitis (2 cases), worsening of infantile acne (1 case), increases of serum cholesterol and triglycerides (4 cases), changes in serum phosphate levels (2 cases), increase of cholinesterase (2 cases), transient neutropenia (2 cases), transient anemia (1 case), transient lymphopenia (1 case) and recurrent infections (7 cases). No grade 3-4 adverse events were reported. Treatment is currently continued in 13/17 patients. Benefits were reported in 14/17 patients and included decrease of cardiac rhabdomyoma size and improvement of arrhythmia, decrease of SEGA size, reduction of seizure frequency and regression of congenital focal lymphedema. Despite everolimus therapy, two patients treated for intractable epilepsy are still experiencing seizures and another one treated for SEGA showed no volume reduction. CONCLUSION: This retrospective multicenter study demonstrates that mTOR inhibitor treatment with everolimus is safe in TSC patients under the age of 2 years and shows beneficial effects on cardiac manifestations, SEGA size and early epilepsy.
ESTHER : Saffari_2019_Orphanet.J.Rare.Dis_14_96
PubMedSearch : Saffari_2019_Orphanet.J.Rare.Dis_14_96
PubMedID: 31053163

Title : The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity - Gartemann_2008_J.Bacteriol_190_2138
Author(s) : Gartemann KH , Abt B , Bekel T , Burger A , Engemann J , Flugel M , Gaigalat L , Goesmann A , Grafen I , Kalinowski J , Kaup O , Kirchner O , Krause L , Linke B , McHardy A , Meyer F , Pohle S , Ruckert C , Schneiker S , Zellermann EM , Puhler A , Eichenlaub R , Kaiser O , Bartels D
Ref : Journal of Bacteriology , 190 :2138 , 2008
Abstract : Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete that causes bacterial wilt and canker of tomato. The nucleotide sequence of the genome of strain NCPPB382 was determined. The chromosome is circular, consists of 3.298 Mb, and has a high G+C content (72.6%). Annotation revealed 3,080 putative protein-encoding sequences; only 26 pseudogenes were detected. Two rrn operons, 45 tRNAs, and three small stable RNA genes were found. The two circular plasmids, pCM1 (27.4 kbp) and pCM2 (70.0 kbp), which carry pathogenicity genes and thus are essential for virulence, have lower G+C contents (66.5 and 67.6%, respectively). In contrast to the genome of the closely related organism Clavibacter michiganensis subsp. sepedonicus, the genome of C. michiganensis subsp. michiganensis lacks complete insertion elements and transposons. The 129-kb chp/tomA region with a low G+C content near the chromosomal origin of replication was shown to be necessary for pathogenicity. This region contains numerous genes encoding proteins involved in uptake and metabolism of sugars and several serine proteases. There is evidence that single genes located in this region, especially genes encoding serine proteases, are required for efficient colonization of the host. Although C. michiganensis subsp. michiganensis grows mainly in the xylem of tomato plants, no evidence for pronounced genome reduction was found. C. michiganensis subsp. michiganensis seems to have as many transporters and regulators as typical soil-inhabiting bacteria. However, the apparent lack of a sulfate reduction pathway, which makes C. michiganensis subsp. michiganensis dependent on reduced sulfur compounds for growth, is probably the reason for the poor survival of C. michiganensis subsp. michiganensis in soil.
ESTHER : Gartemann_2008_J.Bacteriol_190_2138
PubMedSearch : Gartemann_2008_J.Bacteriol_190_2138
PubMedID: 18192381
Gene_locus related to this paper: clam3-a5cln9 , clam3-a5clw2 , clam3-a5cm48 , clam3-a5cmg3 , clam3-a5cmk0 , clam3-a5cn86 , clam3-a5cr52 , clam3-a5cr57 , clam3-a5crq6 , clam3-a5csc5 , clam3-metx , clams-b0rcr0 , clams-b0rdd6 , clams-b0rde2 , clams-b0rga8 , clams-b0riv5 , clam3-a5cui5 , clam3-a5cp43 , clam3-a5cp56

Title : The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis - Vorholter_2008_J.Biotechnol_134_33
Author(s) : Vorholter FJ , Schneiker S , Goesmann A , Krause L , Bekel T , Kaiser O , Linke B , Patschkowski T , Ruckert C , Schmid J , Sidhu VK , Sieber V , Tauch A , Watt SA , Weisshaar B , Becker A , Niehaus K , Puhler A
Ref : J Biotechnol , 134 :33 , 2008
Abstract : The complete genome sequence of the Xanthomonas campestris pv. campestris strain B100 was established. It consisted of a chromosome of 5,079,003bp, with 4471 protein-coding genes and 62 RNA genes. Comparative genomics showed that the genes required for the synthesis of xanthan and xanthan precursors were highly conserved among three sequenced X. campestris pv. campestris genomes, but differed noticeably when compared to the remaining four Xanthomonas genomes available. For the xanthan biosynthesis genes gumB and gumK earlier translational starts were proposed, while gumI and gumL turned out to be unique with no homologues beyond the Xanthomonas genomes sequenced. From the genomic data the biosynthesis pathways for the production of the exopolysaccharide xanthan could be elucidated. The first step of this process is the uptake of sugars serving as carbon and energy sources wherefore genes for 15 carbohydrate import systems could be identified. Metabolic pathways playing a role for xanthan biosynthesis could be deduced from the annotated genome. These reconstructed pathways concerned the storage and metabolization of the imported sugars. The recognized sugar utilization pathways included the Entner-Doudoroff and the pentose phosphate pathway as well as the Embden-Meyerhof pathway (glycolysis). The reconstruction indicated that the nucleotide sugar precursors for xanthan can be converted from intermediates of the pentose phosphate pathway, some of which are also intermediates of glycolysis or the Entner-Doudoroff pathway. Xanthan biosynthesis requires in particular the nucleotide sugars UDP-glucose, UDP-glucuronate, and GDP-mannose, from which xanthan repeat units are built under the control of the gum genes. The updated genome annotation data allowed reconsidering and refining the mechanistic model for xanthan biosynthesis.
ESTHER : Vorholter_2008_J.Biotechnol_134_33
PubMedSearch : Vorholter_2008_J.Biotechnol_134_33
PubMedID: 18304669
Gene_locus related to this paper: xanax-ENTF2 , xanax-GAA , xanax-PTRB , xanax-XAC0515 , xanax-XAC0628 , xanax-XAC0736 , xanax-XAC0753 , xanax-XAC1713 , xanca-acvB , xanca-BIOH , xanca-CATD , xanca-CPO , xanca-estA1 , xanca-impep , xanca-METX , xanca-XCC0080 , xanca-XCC0180 , xanca-XCC0266 , xanca-XCC0843 , xanca-XCC1105 , xanca-XCC1734 , xanca-XCC2285 , xanca-XCC2374 , xanca-XCC2397 , xanca-XCC2405 , xanca-XCC2566 , xanca-XCC2722 , xanca-XCC2817 , xanca-XCC3028 , xanca-XCC3164 , xanca-XCC3219 , xanca-XCC3514 , xanca-XCC3548 , xanca-XCC3555 , xanca-XCC3961 , xanca-XCC3970 , xanca-XCC4016 , xanca-XCC4180 , xanca-XYNB2 , xancb-b0rna3 , xancb-b0rq23

Title : The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae - Gross_2008_BMC.Genomics_9_449
Author(s) : Gross R , Guzman CA , Sebaihia M , dos Santos VA , Pieper DH , Koebnik R , Lechner M , Bartels D , Buhrmester J , Choudhuri JV , Ebensen T , Gaigalat L , Herrmann S , Khachane AN , Larisch C , Link S , Linke B , Meyer F , Mormann S , Nakunst D , Ruckert C , Schneiker-Bekel S , Schulze K , Vorholter FJ , Yevsa T , Engle JT , Goldman WE , Puhler A , Gobel UB , Goesmann A , Blocker H , Kaiser O , Martinez-Arias R
Ref : BMC Genomics , 9 :449 , 2008
Abstract : BACKGROUND: Bordetella petrii is the only environmental species hitherto found among the otherwise host-restricted and pathogenic members of the genus Bordetella. Phylogenetically, it connects the pathogenic Bordetellae and environmental bacteria of the genera Achromobacter and Alcaligenes, which are opportunistic pathogens. B. petrii strains have been isolated from very different environmental niches, including river sediment, polluted soil, marine sponges and a grass root. Recently, clinical isolates associated with bone degenerative disease or cystic fibrosis have also been described. RESULTS: In this manuscript we present the results of the analysis of the completely annotated genome sequence of the B. petrii strain DSMZ12804. B. petrii has a mosaic genome of 5,287,950 bp harboring numerous mobile genetic elements, including seven large genomic islands. Four of them are highly related to the clc element of Pseudomonas knackmussii B13, which encodes genes involved in the degradation of aromatics. Though being an environmental isolate, the sequenced B. petrii strain also encodes proteins related to virulence factors of the pathogenic Bordetellae, including the filamentous hemagglutinin, which is a major colonization factor of B. pertussis, and the master virulence regulator BvgAS. However, it lacks all known toxins of the pathogenic Bordetellae. CONCLUSION: The genomic analysis suggests that B. petrii represents an evolutionary link between free-living environmental bacteria and the host-restricted obligate pathogenic Bordetellae. Its remarkable metabolic versatility may enable B. petrii to thrive in very different ecological niches.
ESTHER : Gross_2008_BMC.Genomics_9_449
PubMedSearch : Gross_2008_BMC.Genomics_9_449
PubMedID: 18826580
Gene_locus related to this paper: alceu-CBNC , borpd-a9hwh7 , borpd-a9hxw5 , borpd-a9hyr5 , borpd-a9hzm8 , borpd-a9i1l8 , borpd-a9i4f4 , borpd-a9i8i7 , borpd-a9icj9 , borpd-a9iec4 , borpd-a9iep7 , borpd-a9ih03 , borpd-a9iu09 , borpd-metx , bursp-tecF , borpd-a9i1v7

Title : Complete genome sequence of the myxobacterium Sorangium cellulosum - Schneiker_2007_Nat.Biotechnol_25_1281
Author(s) : Schneiker S , Perlova O , Kaiser O , Gerth K , Alici A , Altmeyer MO , Bartels D , Bekel T , Beyer S , Bode E , Bode HB , Bolten CJ , Choudhuri JV , Doss S , Elnakady YA , Frank B , Gaigalat L , Goesmann A , Groeger C , Gross F , Jelsbak L , Kalinowski J , Kegler C , Knauber T , Konietzny S , Kopp M , Krause L , Krug D , Linke B , Mahmud T , Martinez-Arias R , McHardy AC , Merai M , Meyer F , Mormann S , Munoz-Dorado J , Perez J , Pradella S , Rachid S , Raddatz G , Rosenau F , Ruckert C , Sasse F , Scharfe M , Schuster SC , Suen G , Treuner-Lange A , Velicer GJ , Vorholter FJ , Weissman KJ , Welch RD , Wenzel SC , Whitworth DE , Wilhelm S , Wittmann C , Blocker H , Puhler A , Muller R
Ref : Nat Biotechnol , 25 :1281 , 2007
Abstract : The genus Sorangium synthesizes approximately half of the secondary metabolites isolated from myxobacteria, including the anti-cancer metabolite epothilone. We report the complete genome sequence of the model Sorangium strain S. cellulosum So ce56, which produces several natural products and has morphological and physiological properties typical of the genus. The circular genome, comprising 13,033,779 base pairs, is the largest bacterial genome sequenced to date. No global synteny with the genome of Myxococcus xanthus is apparent, revealing an unanticipated level of divergence between these myxobacteria. A large percentage of the genome is devoted to regulation, particularly post-translational phosphorylation, which probably supports the strain's complex, social lifestyle. This regulatory network includes the highest number of eukaryotic protein kinase-like kinases discovered in any organism. Seventeen secondary metabolite loci are encoded in the genome, as well as many enzymes with potential utility in industry.
ESTHER : Schneiker_2007_Nat.Biotechnol_25_1281
PubMedSearch : Schneiker_2007_Nat.Biotechnol_25_1281
PubMedID: 17965706
Gene_locus related to this paper: sorc5-a9en84 , sorc5-a9enf0 , sorc5-a9eu04 , sorc5-a9eur8 , sorc5-a9ev31 , sorc5-a9ewe7 , sorc5-a9f2w6 , sorc5-a9fd82 , sorc5-a9fec8 , sorc5-a9fg26 , sorc5-a9fjh7 , sorc5-a9fpe7 , sorc5-a9fvz2 , sorc5-a9fw17 , sorc5-a9fw70 , sorc5-a9fwe6 , sorc5-a9fya1 , sorc5-a9g9n9 , sorc5-a9g651 , sorc5-a9gj93 , sorc5-a9glc5 , sorc5-a9glv5 , sorc5-a9gqy4 , sorc5-a9grj0 , sorc5-a9grk3 , sorc5-a9grp4 , sorc5-a9grt6 , sorc5-a9guw3 , sorc5-a9gy73 , sorce-q2n3s7 , sorc5-a9fsu5 , sorc5-a9gq11 , sorc5-a9ev65 , sorc5-a9fcj4 , sorc5-a9gut3 , sorc5-a9env4 , sorc5-a9fai0 , sorc5-a9g908 , sorc5-a9gmg6 , sorc5-ce1

Title : Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes - Hain_2006_J.Bacteriol_188_7405
Author(s) : Hain T , Steinweg C , Kuenne CT , Billion A , Ghai R , Chatterjee SS , Domann E , Karst U , Goesmann A , Bekel T , Bartels D , Kaiser O , Meyer F , Puhler A , Weisshaar B , Wehland J , Liang C , Dandekar T , Lampidis R , Kreft J , Goebel W , Chakraborty T
Ref : Journal of Bacteriology , 188 :7405 , 2006
Abstract : We present the complete genome sequence of Listeria welshimeri, a nonpathogenic member of the genus Listeria. Listeria welshimeri harbors a circular chromosome of 2,814,130 bp with 2,780 open reading frames. Comparative genomic analysis of chromosomal regions between L. welshimeri, Listeria innocua, and Listeria monocytogenes shows strong overall conservation of synteny, with the exception of the translocation of an F(o)F(1) ATP synthase. The smaller size of the L. welshimeri genome is the result of deletions in all of the genes involved in virulence and of "fitness" genes required for intracellular survival, transcription factors, and LPXTG- and LRR-containing proteins as well as 55 genes involved in carbohydrate transport and metabolism. In total, 482 genes are absent from L. welshimeri relative to L. monocytogenes. Of these, 249 deletions are commonly absent in both L. welshimeri and L. innocua, suggesting similar genome evolutionary paths from an ancestor. We also identified 311 genes specific to L. welshimeri that are absent in the other two species, indicating gene expansion in L. welshimeri, including horizontal gene transfer. The species L. welshimeri appears to have been derived from early evolutionary events and an ancestor more compact than L. monocytogenes that led to the emergence of nonpathogenic Listeria spp.
ESTHER : Hain_2006_J.Bacteriol_188_7405
PubMedSearch : Hain_2006_J.Bacteriol_188_7405
PubMedID: 16936040
Gene_locus related to this paper: lisin-LIN0976 , lisin-LIN2544 , lismo-LMO2089 , lismo-LMO2452 , lismo-LMO2453 , lismo-metx , lisss-d3urb3 , lisw6-a0agy6 , lisw6-a0ajc8 , lisw6-a0am12

Title : Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72 - Krause_2006_Nat.Biotechnol_24_1385
Author(s) : Krause A , Ramakumar A , Bartels D , Battistoni F , Bekel T , Boch J , Bohm M , Friedrich F , Hurek T , Krause L , Linke B , McHardy AC , Sarkar A , Schneiker S , Syed AA , Thauer R , Vorholter FJ , Weidner S , Puhler A , Reinhold-Hurek B , Kaiser O , Goesmann A
Ref : Nat Biotechnol , 24 :1385 , 2006
Abstract : Azoarcus sp. strain BH72, a mutualistic endophyte of rice and other grasses, is of agrobiotechnological interest because it supplies biologically fixed nitrogen to its host and colonizes plants in remarkably high numbers without eliciting disease symptoms. The complete genome sequence is 4,376,040-bp long and contains 3,992 predicted protein-coding sequences. Genome comparison with the Azoarcus-related soil bacterium strain EbN1 revealed a surprisingly low degree of synteny. Coding sequences involved in the synthesis of surface components potentially important for plant-microbe interactions were more closely related to those of plant-associated bacteria. Strain BH72 appears to be 'disarmed' compared to plant pathogens, having only a few enzymes that degrade plant cell walls; it lacks type III and IV secretion systems, related toxins and an N-acyl homoserine lactones-based communication system. The genome contains remarkably few mobile elements, indicating a low rate of recent gene transfer that is presumably due to adaptation to a stable, low-stress microenvironment.
ESTHER : Krause_2006_Nat.Biotechnol_24_1385
PubMedSearch : Krause_2006_Nat.Biotechnol_24_1385
PubMedID: 17057704
Gene_locus related to this paper: azosb-a1k1f9 , azosb-a1k1w3 , azosb-a1k2q2 , azosb-a1k4p7 , azosb-a1k5d3 , azosb-a1k5m5 , azosb-a1k5s0 , azosb-a1k6q2 , azosb-a1k6w9 , azosb-a1k6y0 , azosb-a1k7c8 , azosb-a1k9j2 , azosb-a1k9v9 , azosb-a1k696 , azosb-a1k988 , azosb-a1kbn4 , azosb-metx , azosb-a1kal1 , azosb-a1kal0 , azosb-a1k3c3 , azosb-hboh , azosb-a1kb81

Title : Identification and analysis of the chivosazol biosynthetic gene cluster from the myxobacterial model strain Sorangium cellulosum So ce56 - Perlova_2006_J.Biotechnol_121_174
Author(s) : Perlova O , Gerth K , Kaiser O , Hans A , Muller R
Ref : J Biotechnol , 121 :174 , 2006
Abstract : Myxobacteria belonging to the genus Sorangium are known to produce a variety of biologically active secondary metabolites. Chivosazol is a macrocyclic antibiotic active against yeast, filamentous fungi and especially against mammalian cells. The compound specifically destroys the actin skeleton of eucaryotic cells and does not show activity against bacteria. Chivosazol contains an oxazole ring and a glycosidically bound 6-deoxyglucose (except for chivosazol F). In this paper we describe the biosynthetic gene cluster that directs chivosazol biosynthesis in the model strain Sorangium cellulosum So ce56. This biosynthetic gene cluster spans 92 kbp on the chromosome and contains four polyketide synthase genes and one hybrid polyketide synthase/nonribosomal peptide synthetase gene. An additional gene encoding a protein with similarity to different methyltransferases and presumably involved in post-polyketide modification was identified downstream of the core biosynthetic gene cluster. The chivosazol biosynthetic gene locus belongs to the recently identified and rapidly growing class of trans-acyltransferase polyketide synthases, which do not contain acyltransferase domains integrated into the multimodular megasynthetases.
ESTHER : Perlova_2006_J.Biotechnol_121_174
PubMedSearch : Perlova_2006_J.Biotechnol_121_174
PubMedID: 16313990
Gene_locus related to this paper: sorce-q2n3s7

Title : Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis - Schneiker_2006_Nat.Biotechnol_24_997
Author(s) : Schneiker S , Martins dos Santos VA , Bartels D , Bekel T , Brecht M , Buhrmester J , Chernikova TN , Denaro R , Ferrer M , Gertler C , Goesmann A , Golyshina OV , Kaminski F , Khachane AN , Lang S , Linke B , McHardy AC , Meyer F , Nechitaylo T , Puhler A , Regenhardt D , Rupp O , Sabirova JS , Selbitschka W , Yakimov MM , Timmis KN , Vorholter FJ , Weidner S , Kaiser O , Golyshin PN
Ref : Nat Biotechnol , 24 :997 , 2006
Abstract : Alcanivorax borkumensis is a cosmopolitan marine bacterium that uses oil hydrocarbons as its exclusive source of carbon and energy. Although barely detectable in unpolluted environments, A. borkumensis becomes the dominant microbe in oil-polluted waters. A. borkumensis SK2 has a streamlined genome with a paucity of mobile genetic elements and energy generation-related genes, but with a plethora of genes accounting for its wide hydrocarbon substrate range and efficient oil-degradation capabilities. The genome further specifies systems for scavenging of nutrients, particularly organic and inorganic nitrogen and oligo-elements, biofilm formation at the oil-water interface, biosurfactant production and niche-specific stress responses. The unique combination of these features provides A. borkumensis SK2 with a competitive edge in oil-polluted environments. This genome sequence provides the basis for the future design of strategies to mitigate the ecological damage caused by oil spills.
ESTHER : Schneiker_2006_Nat.Biotechnol_24_997
PubMedSearch : Schneiker_2006_Nat.Biotechnol_24_997
PubMedID: 16878126
Gene_locus related to this paper: alcbo-Q9F9H0 , alcbs-q0vl04 , alcbs-q0vl36 , alcbs-q0vl92 , alcbs-q0vlp5 , alcbs-q0vlq1 , alcbs-q0vlt9 , alcbs-q0vm92 , alcbs-q0vmd2 , alcbs-q0vmd6 , alcbs-q0vmn9 , alcbs-q0vnu3 , alcbs-q0vp43 , alcbs-q0vpa9 , alcbs-q0vpc7 , alcbs-q0vpg7 , alcbs-q0vpn2 , alcbs-q0vps0 , alcbs-q0vq49 , alcbs-q0vsg4 , alcbs-q0vth9 , marav-a1u5n0 , alcbs-q0vp59 , alcbs-q0vlk5

Title : Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence - Thieme_2005_J.Bacteriol_187_7254
Author(s) : Thieme F , Koebnik R , Bekel T , Berger C , Boch J , Buttner D , Caldana C , Gaigalat L , Goesmann A , Kay S , Kirchner O , Lanz C , Linke B , McHardy AC , Meyer F , Mittenhuber G , Nies DH , Niesbach-Klosgen U , Patschkowski T , Ruckert C , Rupp O , Schneiker S , Schuster SC , Vorholter FJ , Weber E , Puhler A , Bonas U , Bartels D , Kaiser O
Ref : Journal of Bacteriology , 187 :7254 , 2005
Abstract : The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically important yield losses. This pathosystem has become a well-established model for studying bacterial infection strategies. Here, we present the whole-genome sequence of the pepper-pathogenic Xanthomonas campestris pv. vesicatoria strain 85-10, which comprises a 5.17-Mb circular chromosome and four plasmids. The genome has a high G+C content (64.75%) and signatures of extensive genome plasticity. Whole-genome comparisons revealed a gene order similar to both Xanthomonas axonopodis pv. citri and Xanthomonas campestris pv. campestris and a structure completely different from Xanthomonas oryzae pv. oryzae. A total of 548 coding sequences (12.2%) are unique to X. campestris pv. vesicatoria. In addition to a type III secretion system, which is essential for pathogenicity, the genome of strain 85-10 encodes all other types of protein secretion systems described so far in gram-negative bacteria. Remarkably, one of the putative type IV secretion systems encoded on the largest plasmid is similar to the Icm/Dot systems of the human pathogens Legionella pneumophila and Coxiella burnetii. Comparisons with other completely sequenced plant pathogens predicted six novel type III effector proteins and several other virulence factors, including adhesins, cell wall-degrading enzymes, and extracellular polysaccharides.
ESTHER : Thieme_2005_J.Bacteriol_187_7254
PubMedSearch : Thieme_2005_J.Bacteriol_187_7254
PubMedID: 16237009
Gene_locus related to this paper: xanac-q8pmm6 , xanax-ACVB , xanax-BIOH , xanax-CATD , xanax-CPO , xanax-DHAA , xanax-ENTF2 , xanax-GAA , xanax-META , xanax-METX , xanax-PCAD , xanax-PHBC , xanax-PTRB , xanax-Q8PMQ8 , xanax-XAC0198 , xanax-XAC0262 , xanax-XAC0319 , xanax-XAC0372 , xanax-XAC0375 , xanax-XAC0515 , xanax-XAC0574 , xanax-XAC0591 , xanax-XAC0619 , xanax-XAC0628 , xanax-XAC0736 , xanax-XAC0753 , xanax-XAC0805 , xanax-XAC0874 , xanax-XAC1200 , xanax-XAC1213 , xanax-XAC1713 , xanax-XAC2532 , xanax-XAC2541 , xanax-XAC2907 , xanax-XAC2981 , xanax-XAC2990 , xanax-XAC3037 , xanax-XAC3053 , xanax-XAC3152 , xanax-XAC3770 , xanax-XAC3999 , xanax-XAC4046 , xanax-XAC4055 , xanax-XAC4106 , xanax-XAC4221 , xanax-XAC4316 , xanax-XYNB , xanc5-q3bqi2 , xanc5-q3bst4 , xanc5-q3btn4 , xanc5-q3bup9 , xanc5-q3byx0 , xanca-estA1 , xanca-impep , xanca-XCC1105 , xanca-XCC2285 , xanca-XCC2566 , xanca-XCC2722 , xanca-XCC3164 , xanca-XCC3555 , xanor-q5h3e8

Title : Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora - Tauch_2005_J.Bacteriol_187_4671
Author(s) : Tauch A , Kaiser O , Hain T , Goesmann A , Weisshaar B , Albersmeier A , Bekel T , Bischoff N , Brune I , Chakraborty T , Kalinowski J , Meyer F , Rupp O , Schneiker S , Viehoever P , Puhler A
Ref : Journal of Bacteriology , 187 :4671 , 2005
Abstract : Corynebacterium jeikeium is a "lipophilic" and multidrug-resistant bacterial species of the human skin flora that has been recognized with increasing frequency as a serious nosocomial pathogen. Here we report the genome sequence of the clinical isolate C. jeikeium K411, which was initially recovered from the axilla of a bone marrow transplant patient. The genome of C. jeikeium K411 consists of a circular chromosome of 2,462,499 bp and the 14,323-bp bacteriocin-producing plasmid pKW4. The chromosome of C. jeikeium K411 contains 2,104 predicted coding sequences, 52% of which were considered to be orthologous with genes in the Corynebacterium glutamicum, Corynebacterium efficiens, and Corynebacterium diphtheriae genomes. These genes apparently represent the chromosomal backbone that is conserved between the four corynebacteria. Among the genes that lack an ortholog in the known corynebacterial genomes, many are located close to transposable elements or revealed an atypical G+C content, indicating that horizontal gene transfer played an important role in the acquisition of genes involved in iron and manganese homeostasis, in multidrug resistance, in bacterium-host interaction, and in virulence. Metabolic analyses of the genome sequence indicated that the "lipophilic" phenotype of C. jeikeium most likely originates from the absence of fatty acid synthase and thus represents a fatty acid auxotrophy. Accordingly, both the complete gene repertoire and the deduced lifestyle of C. jeikeium K411 largely reflect the strict dependence of growth on the presence of exogenous fatty acids. The predicted virulence factors of C. jeikeium K411 are apparently involved in ensuring the availability of exogenous fatty acids by damaging the host tissue.
ESTHER : Tauch_2005_J.Bacteriol_187_4671
PubMedSearch : Tauch_2005_J.Bacteriol_187_4671
PubMedID: 15968079
Gene_locus related to this paper: corjk-q4jsq8 , corjk-q4jst9 , corjk-q4jt28 , corjk-q4jt69 , corjk-q4jta2 , corjk-q4jth4 , corjk-q4jti5 , corjk-q4ju26 , corjk-q4jug4 , corjk-q4juz4 , corjk-q4jv41 , corjk-q4jvr2 , corjk-q4jwi5 , corjk-q4jwq5 , corjk-q4jwu9 , corjk-q4jx57 , corjk-q4jx58 , corjk-q4jxf4 , corjk-q4jxg1 , corjk-q4jxi0 , corjk-q4jxk5 , corjk-q4jxw6 , corjk-q4jxx7 , corjk-q4jy17 , corjk-q4jy19 , corjk-q4jy21 , corjk-q4jy22 , corjk-q4jyd0 , corjk-q4jyf2