Bekel T

References (11)

Title : The complete genome sequence of the dominant Sinorhizobium meliloti field isolate SM11 extends the S. meliloti pan-genome - Schneiker-Bekel_2011_J.Biotechnol_155_20
Author(s) : Schneiker-Bekel S , Wibberg D , Bekel T , Blom J , Linke B , Neuweger H , Stiens M , Vorholter FJ , Weidner S , Goesmann A , Puhler A , Schluter A
Ref : J Biotechnol , 155 :20 , 2011
Abstract : Isolates of the symbiotic nitrogen-fixing species Sinorhizobium meliloti usually contain a chromosome and two large megaplasmids encoding functions that are absolutely required for the specific interaction of the microsymbiont with corresponding host plants leading to an effective symbiosis. The complete genome sequence, including the megaplasmids pSmeSM11c (related to pSymA) and pSmeSM11d (related to pSymB), was established for the dominant, indigenous S. meliloti strain SM11 that had been isolated during a long-term field release experiment with genetically modified S. meliloti strains. The chromosome, the largest replicon of S. meliloti SM11, is 3,908,022bp in size and codes for 3785 predicted protein coding sequences. The size of megaplasmid pSmeSM11c is 1,633,319bp and it contains 1760 predicted protein coding sequences whereas megaplasmid pSmeSM11d is 1,632,395bp in size and comprises 1548 predicted coding sequences. The gene content of the SM11 chromosome is quite similar to that of the reference strain S. meliloti Rm1021. Comparison of pSmeSM11c to pSymA of the reference strain revealed that many gene regions of these replicons are variable, supporting the assessment that pSymA is a major hot-spot for intra-specific differentiation. Plasmids pSymA and pSmeSM11c both encode unique genes. Large gene regions of pSmeSM11c are closely related to corresponding parts of Sinorhizobium medicae WSM419 plasmids. Moreover, pSmeSM11c encodes further novel gene regions, e.g. additional plasmid survival genes (partition, mobilisation and conjugative transfer genes), acdS encoding 1-aminocyclopropane-1-carboxylate deaminase involved in modulation of the phytohormone ethylene level and genes having predicted functions in degradative capabilities, stress response, amino acid metabolism and associated pathways. In contrast to Rm1021 pSymA and pSmeSM11c, megaplasmid pSymB of strain Rm1021 and pSmeSM11d are highly conserved showing extensive synteny with only few rearrangements. Most remarkably, pSmeSM11b contains a new gene cluster predicted to be involved in polysaccharide biosynthesis. Compilation of the S. meliloti SM11 genome sequence contributes to an extension of the S. meliloti pan-genome.
ESTHER : Schneiker-Bekel_2011_J.Biotechnol_155_20
PubMedSearch : Schneiker-Bekel_2011_J.Biotechnol_155_20
PubMedID: 21396969
Gene_locus related to this paper: rhime-PCAD , rhime-RB1395

Title : Complete genome sequence and lifestyle of black-pigmented Corynebacterium aurimucosum ATCC 700975 (formerly C. nigricans CN-1) isolated from a vaginal swab of a woman with spontaneous abortion - Trost_2010_BMC.Genomics_11_91
Author(s) : Trost E , Gotker S , Schneider J , Schneiker-Bekel S , Szczepanowski R , Tilker A , Viehoever P , Arnold W , Bekel T , Blom J , Gartemann KH , Linke B , Goesmann A , Puhler A , Shukla SK , Tauch A
Ref : BMC Genomics , 11 :91 , 2010
Abstract : BACKGROUND: Corynebacterium aurimucosum is a slightly yellowish, non-lipophilic, facultative anaerobic member of the genus Corynebacterium and predominantly isolated from human clinical specimens. Unusual black-pigmented variants of C. aurimucosum (originally named as C. nigricans) continue to be recovered from the female urogenital tract and they are associated with complications during pregnancy. C. aurimucosum ATCC 700975 (C. nigricans CN-1) was originally isolated from a vaginal swab of a 34-year-old woman who experienced a spontaneous abortion during month six of pregnancy. For a better understanding of the physiology and lifestyle of this potential urogenital pathogen, the complete genome sequence of C. aurimucosum ATCC 700975 was determined.
RESULTS: Sequencing and assembly of the C. aurimucosum ATCC 700975 genome yielded a circular chromosome of 2,790,189 bp in size and the 29,037-bp plasmid pET44827. Specific gene sets associated with the central metabolism of C. aurimucosum apparently provide enhanced metabolic flexibility and adaptability in aerobic, anaerobic and low-pH environments, including gene clusters for the uptake and degradation of aromatic amines, L-histidine and L-tartrate as well as a gene region for the formation of selenocysteine and its incorporation into formate dehydrogenase. Plasmid pET44827 codes for a non-ribosomal peptide synthetase that plays the pivotal role in the synthesis of the characteristic black pigment of C. aurimucosum ATCC 700975.
CONCLUSIONS: The data obtained by the genome project suggest that C. aurimucosum could be both a resident of the human gut and possibly a pathogen in the female genital tract causing complications during pregnancy. Since hitherto all black-pigmented C. aurimucosum strains have been recovered from female genital source, biosynthesis of the pigment is apparently required for colonization by protecting the bacterial cells against the high hydrogen peroxide concentration in the vaginal environment. The location of the corresponding genes on plasmid pET44827 explains why black-pigmented (formerly C. nigricans) and non-pigmented C. aurimucosum strains were isolated from clinical specimens.
ESTHER : Trost_2010_BMC.Genomics_11_91
PubMedSearch : Trost_2010_BMC.Genomics_11_91
PubMedID: 20137072
Gene_locus related to this paper: cora7-c3peg4 , cora7-c3pft2 , cora7-c3pgf2 , cora7-c3ph31 , cora7-c3phx8 , cora7-c3piw1 , cora7-c3pix7 , cora7-c3pj13 , cora7-c3pjc9 , cora7-c3pjm1 , cora7-c3pju4 , cora7-c3pjx9 , cora7-c3pjy0 , cora7-c3pk62 , cora7-c3pk63 , cora7-c3pk94 , cora7-c3pk60 , cora7-c3phz6 , cora7-c3phz5 , cora7-c3pim5 , cora7-c3pjw4

Title : The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity - Gartemann_2008_J.Bacteriol_190_2138
Author(s) : Gartemann KH , Abt B , Bekel T , Burger A , Engemann J , Flugel M , Gaigalat L , Goesmann A , Grafen I , Kalinowski J , Kaup O , Kirchner O , Krause L , Linke B , McHardy A , Meyer F , Pohle S , Ruckert C , Schneiker S , Zellermann EM , Puhler A , Eichenlaub R , Kaiser O , Bartels D
Ref : Journal of Bacteriology , 190 :2138 , 2008
Abstract : Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete that causes bacterial wilt and canker of tomato. The nucleotide sequence of the genome of strain NCPPB382 was determined. The chromosome is circular, consists of 3.298 Mb, and has a high G+C content (72.6%). Annotation revealed 3,080 putative protein-encoding sequences; only 26 pseudogenes were detected. Two rrn operons, 45 tRNAs, and three small stable RNA genes were found. The two circular plasmids, pCM1 (27.4 kbp) and pCM2 (70.0 kbp), which carry pathogenicity genes and thus are essential for virulence, have lower G+C contents (66.5 and 67.6%, respectively). In contrast to the genome of the closely related organism Clavibacter michiganensis subsp. sepedonicus, the genome of C. michiganensis subsp. michiganensis lacks complete insertion elements and transposons. The 129-kb chp/tomA region with a low G+C content near the chromosomal origin of replication was shown to be necessary for pathogenicity. This region contains numerous genes encoding proteins involved in uptake and metabolism of sugars and several serine proteases. There is evidence that single genes located in this region, especially genes encoding serine proteases, are required for efficient colonization of the host. Although C. michiganensis subsp. michiganensis grows mainly in the xylem of tomato plants, no evidence for pronounced genome reduction was found. C. michiganensis subsp. michiganensis seems to have as many transporters and regulators as typical soil-inhabiting bacteria. However, the apparent lack of a sulfate reduction pathway, which makes C. michiganensis subsp. michiganensis dependent on reduced sulfur compounds for growth, is probably the reason for the poor survival of C. michiganensis subsp. michiganensis in soil.
ESTHER : Gartemann_2008_J.Bacteriol_190_2138
PubMedSearch : Gartemann_2008_J.Bacteriol_190_2138
PubMedID: 18192381
Gene_locus related to this paper: clam3-a5cln9 , clam3-a5clw2 , clam3-a5cm48 , clam3-a5cmg3 , clam3-a5cmk0 , clam3-a5cn86 , clam3-a5cr52 , clam3-a5cr57 , clam3-a5crq6 , clam3-a5csc5 , clam3-metx , clams-b0rcr0 , clams-b0rdd6 , clams-b0rde2 , clams-b0rga8 , clams-b0riv5 , clam3-a5cui5 , clam3-a5cp43 , clam3-a5cp56

Title : The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing - Tauch_2008_J.Biotechnol_136_11
Author(s) : Tauch A , Trost E , Tilker A , Ludewig U , Schneiker S , Goesmann A , Arnold W , Bekel T , Brinkrolf K , Brune I , Gotker S , Kalinowski J , Kamp PB , Lobo FP , Viehoever P , Weisshaar B , Soriano F , Droge M , Puhler A
Ref : J Biotechnol , 136 :11 , 2008
Abstract : Corynebacterium urealyticum is a lipid-requiring, urealytic bacterium of the human skin flora that has been recognized as causative agent of urinary tract infections. We report the analysis of the complete genome sequence of C. urealyticum DSM7109, which was initially recovered from a patient with alkaline-encrusted cystitis. The genome sequence was determined by a combination of pyrosequencing and Sanger technology. The chromosome of C. urealyticum DSM7109 has a size of 2,369,219bp and contains 2024 predicted coding sequences, of which 78% were considered as orthologous with genes in the Corynebacterium jeikeium K411 genome. Metabolic analysis of the lipid-requiring phenotype revealed the absence of a fatty acid synthase gene and the presence of a beta-oxidation pathway along with a large repertoire of auxillary genes for the degradation of exogenous fatty acids. A urease locus with the gene order ureABCEFGD may play a pivotal role in virulence of C. urealyticum by the alkalinization of human urine and the formation of struvite stones. Multidrug resistance of C. urealyticum DSM7109 is mediated by transposable elements, conferring resistances to macrolides, lincosamides, ketolides, aminoglycosides, chloramphenicol, and tetracycline. The complete genome sequence of C. urealyticum revealed a detailed picture of the lifestyle of this opportunistic human pathogen.
ESTHER : Tauch_2008_J.Biotechnol_136_11
PubMedSearch : Tauch_2008_J.Biotechnol_136_11
PubMedID: 18367281
Gene_locus related to this paper: corjk-q4jta2 , coru7-b1vdx7 , coru7-b1vec6 , coru7-b1vec7 , coru7-b1vgb2 , coru7-b1vhj3 , coru7-b1vhm7 , coru7-b1vhw7 , coru7-b1vi01 , coru7-b1vi11 , coru7-b1vi21 , coru7-b1vig1 , coru7-b1vik9 , coru7-metx , coru7-b1vec9 , coru7-b1vix1

Title : The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis - Vorholter_2008_J.Biotechnol_134_33
Author(s) : Vorholter FJ , Schneiker S , Goesmann A , Krause L , Bekel T , Kaiser O , Linke B , Patschkowski T , Ruckert C , Schmid J , Sidhu VK , Sieber V , Tauch A , Watt SA , Weisshaar B , Becker A , Niehaus K , Puhler A
Ref : J Biotechnol , 134 :33 , 2008
Abstract : The complete genome sequence of the Xanthomonas campestris pv. campestris strain B100 was established. It consisted of a chromosome of 5,079,003bp, with 4471 protein-coding genes and 62 RNA genes. Comparative genomics showed that the genes required for the synthesis of xanthan and xanthan precursors were highly conserved among three sequenced X. campestris pv. campestris genomes, but differed noticeably when compared to the remaining four Xanthomonas genomes available. For the xanthan biosynthesis genes gumB and gumK earlier translational starts were proposed, while gumI and gumL turned out to be unique with no homologues beyond the Xanthomonas genomes sequenced. From the genomic data the biosynthesis pathways for the production of the exopolysaccharide xanthan could be elucidated. The first step of this process is the uptake of sugars serving as carbon and energy sources wherefore genes for 15 carbohydrate import systems could be identified. Metabolic pathways playing a role for xanthan biosynthesis could be deduced from the annotated genome. These reconstructed pathways concerned the storage and metabolization of the imported sugars. The recognized sugar utilization pathways included the Entner-Doudoroff and the pentose phosphate pathway as well as the Embden-Meyerhof pathway (glycolysis). The reconstruction indicated that the nucleotide sugar precursors for xanthan can be converted from intermediates of the pentose phosphate pathway, some of which are also intermediates of glycolysis or the Entner-Doudoroff pathway. Xanthan biosynthesis requires in particular the nucleotide sugars UDP-glucose, UDP-glucuronate, and GDP-mannose, from which xanthan repeat units are built under the control of the gum genes. The updated genome annotation data allowed reconsidering and refining the mechanistic model for xanthan biosynthesis.
ESTHER : Vorholter_2008_J.Biotechnol_134_33
PubMedSearch : Vorholter_2008_J.Biotechnol_134_33
PubMedID: 18304669
Gene_locus related to this paper: xanax-ENTF2 , xanax-GAA , xanax-PTRB , xanax-XAC0515 , xanax-XAC0628 , xanax-XAC0736 , xanax-XAC0753 , xanax-XAC1713 , xanca-acvB , xanca-BIOH , xanca-CATD , xanca-CPO , xanca-estA1 , xanca-impep , xanca-METX , xanca-XCC0080 , xanca-XCC0180 , xanca-XCC0266 , xanca-XCC0843 , xanca-XCC1105 , xanca-XCC1734 , xanca-XCC2285 , xanca-XCC2374 , xanca-XCC2397 , xanca-XCC2405 , xanca-XCC2566 , xanca-XCC2722 , xanca-XCC2817 , xanca-XCC3028 , xanca-XCC3164 , xanca-XCC3219 , xanca-XCC3514 , xanca-XCC3548 , xanca-XCC3555 , xanca-XCC3961 , xanca-XCC3970 , xanca-XCC4016 , xanca-XCC4180 , xanca-XYNB2 , xancb-b0rna3 , xancb-b0rq23

Title : Complete genome sequence of the myxobacterium Sorangium cellulosum - Schneiker_2007_Nat.Biotechnol_25_1281
Author(s) : Schneiker S , Perlova O , Kaiser O , Gerth K , Alici A , Altmeyer MO , Bartels D , Bekel T , Beyer S , Bode E , Bode HB , Bolten CJ , Choudhuri JV , Doss S , Elnakady YA , Frank B , Gaigalat L , Goesmann A , Groeger C , Gross F , Jelsbak L , Kalinowski J , Kegler C , Knauber T , Konietzny S , Kopp M , Krause L , Krug D , Linke B , Mahmud T , Martinez-Arias R , McHardy AC , Merai M , Meyer F , Mormann S , Munoz-Dorado J , Perez J , Pradella S , Rachid S , Raddatz G , Rosenau F , Ruckert C , Sasse F , Scharfe M , Schuster SC , Suen G , Treuner-Lange A , Velicer GJ , Vorholter FJ , Weissman KJ , Welch RD , Wenzel SC , Whitworth DE , Wilhelm S , Wittmann C , Blocker H , Puhler A , Muller R
Ref : Nat Biotechnol , 25 :1281 , 2007
Abstract : The genus Sorangium synthesizes approximately half of the secondary metabolites isolated from myxobacteria, including the anti-cancer metabolite epothilone. We report the complete genome sequence of the model Sorangium strain S. cellulosum So ce56, which produces several natural products and has morphological and physiological properties typical of the genus. The circular genome, comprising 13,033,779 base pairs, is the largest bacterial genome sequenced to date. No global synteny with the genome of Myxococcus xanthus is apparent, revealing an unanticipated level of divergence between these myxobacteria. A large percentage of the genome is devoted to regulation, particularly post-translational phosphorylation, which probably supports the strain's complex, social lifestyle. This regulatory network includes the highest number of eukaryotic protein kinase-like kinases discovered in any organism. Seventeen secondary metabolite loci are encoded in the genome, as well as many enzymes with potential utility in industry.
ESTHER : Schneiker_2007_Nat.Biotechnol_25_1281
PubMedSearch : Schneiker_2007_Nat.Biotechnol_25_1281
PubMedID: 17965706
Gene_locus related to this paper: sorc5-a9en84 , sorc5-a9enf0 , sorc5-a9eu04 , sorc5-a9eur8 , sorc5-a9ev31 , sorc5-a9ewe7 , sorc5-a9f2w6 , sorc5-a9fd82 , sorc5-a9fec8 , sorc5-a9fg26 , sorc5-a9fjh7 , sorc5-a9fpe7 , sorc5-a9fvz2 , sorc5-a9fw17 , sorc5-a9fw70 , sorc5-a9fwe6 , sorc5-a9fya1 , sorc5-a9g9n9 , sorc5-a9g651 , sorc5-a9gj93 , sorc5-a9glc5 , sorc5-a9glv5 , sorc5-a9gqy4 , sorc5-a9grj0 , sorc5-a9grk3 , sorc5-a9grp4 , sorc5-a9grt6 , sorc5-a9guw3 , sorc5-a9gy73 , sorce-q2n3s7 , sorc5-a9fsu5 , sorc5-a9gq11 , sorc5-a9ev65 , sorc5-a9fcj4 , sorc5-a9gut3 , sorc5-a9env4 , sorc5-a9fai0 , sorc5-a9g908 , sorc5-a9gmg6 , sorc5-ce1

Title : Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes - Hain_2006_J.Bacteriol_188_7405
Author(s) : Hain T , Steinweg C , Kuenne CT , Billion A , Ghai R , Chatterjee SS , Domann E , Karst U , Goesmann A , Bekel T , Bartels D , Kaiser O , Meyer F , Puhler A , Weisshaar B , Wehland J , Liang C , Dandekar T , Lampidis R , Kreft J , Goebel W , Chakraborty T
Ref : Journal of Bacteriology , 188 :7405 , 2006
Abstract : We present the complete genome sequence of Listeria welshimeri, a nonpathogenic member of the genus Listeria. Listeria welshimeri harbors a circular chromosome of 2,814,130 bp with 2,780 open reading frames. Comparative genomic analysis of chromosomal regions between L. welshimeri, Listeria innocua, and Listeria monocytogenes shows strong overall conservation of synteny, with the exception of the translocation of an F(o)F(1) ATP synthase. The smaller size of the L. welshimeri genome is the result of deletions in all of the genes involved in virulence and of "fitness" genes required for intracellular survival, transcription factors, and LPXTG- and LRR-containing proteins as well as 55 genes involved in carbohydrate transport and metabolism. In total, 482 genes are absent from L. welshimeri relative to L. monocytogenes. Of these, 249 deletions are commonly absent in both L. welshimeri and L. innocua, suggesting similar genome evolutionary paths from an ancestor. We also identified 311 genes specific to L. welshimeri that are absent in the other two species, indicating gene expansion in L. welshimeri, including horizontal gene transfer. The species L. welshimeri appears to have been derived from early evolutionary events and an ancestor more compact than L. monocytogenes that led to the emergence of nonpathogenic Listeria spp.
ESTHER : Hain_2006_J.Bacteriol_188_7405
PubMedSearch : Hain_2006_J.Bacteriol_188_7405
PubMedID: 16936040
Gene_locus related to this paper: lisin-LIN0976 , lisin-LIN2544 , lismo-LMO2089 , lismo-LMO2452 , lismo-LMO2453 , lismo-metx , lisss-d3urb3 , lisw6-a0agy6 , lisw6-a0ajc8 , lisw6-a0am12

Title : Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72 - Krause_2006_Nat.Biotechnol_24_1385
Author(s) : Krause A , Ramakumar A , Bartels D , Battistoni F , Bekel T , Boch J , Bohm M , Friedrich F , Hurek T , Krause L , Linke B , McHardy AC , Sarkar A , Schneiker S , Syed AA , Thauer R , Vorholter FJ , Weidner S , Puhler A , Reinhold-Hurek B , Kaiser O , Goesmann A
Ref : Nat Biotechnol , 24 :1385 , 2006
Abstract : Azoarcus sp. strain BH72, a mutualistic endophyte of rice and other grasses, is of agrobiotechnological interest because it supplies biologically fixed nitrogen to its host and colonizes plants in remarkably high numbers without eliciting disease symptoms. The complete genome sequence is 4,376,040-bp long and contains 3,992 predicted protein-coding sequences. Genome comparison with the Azoarcus-related soil bacterium strain EbN1 revealed a surprisingly low degree of synteny. Coding sequences involved in the synthesis of surface components potentially important for plant-microbe interactions were more closely related to those of plant-associated bacteria. Strain BH72 appears to be 'disarmed' compared to plant pathogens, having only a few enzymes that degrade plant cell walls; it lacks type III and IV secretion systems, related toxins and an N-acyl homoserine lactones-based communication system. The genome contains remarkably few mobile elements, indicating a low rate of recent gene transfer that is presumably due to adaptation to a stable, low-stress microenvironment.
ESTHER : Krause_2006_Nat.Biotechnol_24_1385
PubMedSearch : Krause_2006_Nat.Biotechnol_24_1385
PubMedID: 17057704
Gene_locus related to this paper: azosb-a1k1f9 , azosb-a1k1w3 , azosb-a1k2q2 , azosb-a1k4p7 , azosb-a1k5d3 , azosb-a1k5m5 , azosb-a1k5s0 , azosb-a1k6q2 , azosb-a1k6w9 , azosb-a1k6y0 , azosb-a1k7c8 , azosb-a1k9j2 , azosb-a1k9v9 , azosb-a1k696 , azosb-a1k988 , azosb-a1kbn4 , azosb-metx , azosb-a1kal1 , azosb-a1kal0 , azosb-a1k3c3 , azosb-hboh , azosb-a1kb81

Title : Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis - Schneiker_2006_Nat.Biotechnol_24_997
Author(s) : Schneiker S , Martins dos Santos VA , Bartels D , Bekel T , Brecht M , Buhrmester J , Chernikova TN , Denaro R , Ferrer M , Gertler C , Goesmann A , Golyshina OV , Kaminski F , Khachane AN , Lang S , Linke B , McHardy AC , Meyer F , Nechitaylo T , Puhler A , Regenhardt D , Rupp O , Sabirova JS , Selbitschka W , Yakimov MM , Timmis KN , Vorholter FJ , Weidner S , Kaiser O , Golyshin PN
Ref : Nat Biotechnol , 24 :997 , 2006
Abstract : Alcanivorax borkumensis is a cosmopolitan marine bacterium that uses oil hydrocarbons as its exclusive source of carbon and energy. Although barely detectable in unpolluted environments, A. borkumensis becomes the dominant microbe in oil-polluted waters. A. borkumensis SK2 has a streamlined genome with a paucity of mobile genetic elements and energy generation-related genes, but with a plethora of genes accounting for its wide hydrocarbon substrate range and efficient oil-degradation capabilities. The genome further specifies systems for scavenging of nutrients, particularly organic and inorganic nitrogen and oligo-elements, biofilm formation at the oil-water interface, biosurfactant production and niche-specific stress responses. The unique combination of these features provides A. borkumensis SK2 with a competitive edge in oil-polluted environments. This genome sequence provides the basis for the future design of strategies to mitigate the ecological damage caused by oil spills.
ESTHER : Schneiker_2006_Nat.Biotechnol_24_997
PubMedSearch : Schneiker_2006_Nat.Biotechnol_24_997
PubMedID: 16878126
Gene_locus related to this paper: alcbo-Q9F9H0 , alcbs-q0vl04 , alcbs-q0vl36 , alcbs-q0vl92 , alcbs-q0vlp5 , alcbs-q0vlq1 , alcbs-q0vlt9 , alcbs-q0vm92 , alcbs-q0vmd2 , alcbs-q0vmd6 , alcbs-q0vmn9 , alcbs-q0vnu3 , alcbs-q0vp43 , alcbs-q0vpa9 , alcbs-q0vpc7 , alcbs-q0vpg7 , alcbs-q0vpn2 , alcbs-q0vps0 , alcbs-q0vq49 , alcbs-q0vsg4 , alcbs-q0vth9 , marav-a1u5n0 , alcbs-q0vp59 , alcbs-q0vlk5

Title : Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence - Thieme_2005_J.Bacteriol_187_7254
Author(s) : Thieme F , Koebnik R , Bekel T , Berger C , Boch J , Buttner D , Caldana C , Gaigalat L , Goesmann A , Kay S , Kirchner O , Lanz C , Linke B , McHardy AC , Meyer F , Mittenhuber G , Nies DH , Niesbach-Klosgen U , Patschkowski T , Ruckert C , Rupp O , Schneiker S , Schuster SC , Vorholter FJ , Weber E , Puhler A , Bonas U , Bartels D , Kaiser O
Ref : Journal of Bacteriology , 187 :7254 , 2005
Abstract : The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically important yield losses. This pathosystem has become a well-established model for studying bacterial infection strategies. Here, we present the whole-genome sequence of the pepper-pathogenic Xanthomonas campestris pv. vesicatoria strain 85-10, which comprises a 5.17-Mb circular chromosome and four plasmids. The genome has a high G+C content (64.75%) and signatures of extensive genome plasticity. Whole-genome comparisons revealed a gene order similar to both Xanthomonas axonopodis pv. citri and Xanthomonas campestris pv. campestris and a structure completely different from Xanthomonas oryzae pv. oryzae. A total of 548 coding sequences (12.2%) are unique to X. campestris pv. vesicatoria. In addition to a type III secretion system, which is essential for pathogenicity, the genome of strain 85-10 encodes all other types of protein secretion systems described so far in gram-negative bacteria. Remarkably, one of the putative type IV secretion systems encoded on the largest plasmid is similar to the Icm/Dot systems of the human pathogens Legionella pneumophila and Coxiella burnetii. Comparisons with other completely sequenced plant pathogens predicted six novel type III effector proteins and several other virulence factors, including adhesins, cell wall-degrading enzymes, and extracellular polysaccharides.
ESTHER : Thieme_2005_J.Bacteriol_187_7254
PubMedSearch : Thieme_2005_J.Bacteriol_187_7254
PubMedID: 16237009
Gene_locus related to this paper: xanac-q8pmm6 , xanax-ACVB , xanax-BIOH , xanax-CATD , xanax-CPO , xanax-DHAA , xanax-ENTF2 , xanax-GAA , xanax-META , xanax-METX , xanax-PCAD , xanax-PHBC , xanax-PTRB , xanax-Q8PMQ8 , xanax-XAC0198 , xanax-XAC0262 , xanax-XAC0319 , xanax-XAC0372 , xanax-XAC0375 , xanax-XAC0515 , xanax-XAC0574 , xanax-XAC0591 , xanax-XAC0619 , xanax-XAC0628 , xanax-XAC0736 , xanax-XAC0753 , xanax-XAC0805 , xanax-XAC0874 , xanax-XAC1200 , xanax-XAC1213 , xanax-XAC1713 , xanax-XAC2532 , xanax-XAC2541 , xanax-XAC2907 , xanax-XAC2981 , xanax-XAC2990 , xanax-XAC3037 , xanax-XAC3053 , xanax-XAC3152 , xanax-XAC3770 , xanax-XAC3999 , xanax-XAC4046 , xanax-XAC4055 , xanax-XAC4106 , xanax-XAC4221 , xanax-XAC4316 , xanax-XYNB , xanc5-q3bqi2 , xanc5-q3bst4 , xanc5-q3btn4 , xanc5-q3bup9 , xanc5-q3byx0 , xanca-estA1 , xanca-impep , xanca-XCC1105 , xanca-XCC2285 , xanca-XCC2566 , xanca-XCC2722 , xanca-XCC3164 , xanca-XCC3555 , xanor-q5h3e8

Title : Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora - Tauch_2005_J.Bacteriol_187_4671
Author(s) : Tauch A , Kaiser O , Hain T , Goesmann A , Weisshaar B , Albersmeier A , Bekel T , Bischoff N , Brune I , Chakraborty T , Kalinowski J , Meyer F , Rupp O , Schneiker S , Viehoever P , Puhler A
Ref : Journal of Bacteriology , 187 :4671 , 2005
Abstract : Corynebacterium jeikeium is a "lipophilic" and multidrug-resistant bacterial species of the human skin flora that has been recognized with increasing frequency as a serious nosocomial pathogen. Here we report the genome sequence of the clinical isolate C. jeikeium K411, which was initially recovered from the axilla of a bone marrow transplant patient. The genome of C. jeikeium K411 consists of a circular chromosome of 2,462,499 bp and the 14,323-bp bacteriocin-producing plasmid pKW4. The chromosome of C. jeikeium K411 contains 2,104 predicted coding sequences, 52% of which were considered to be orthologous with genes in the Corynebacterium glutamicum, Corynebacterium efficiens, and Corynebacterium diphtheriae genomes. These genes apparently represent the chromosomal backbone that is conserved between the four corynebacteria. Among the genes that lack an ortholog in the known corynebacterial genomes, many are located close to transposable elements or revealed an atypical G+C content, indicating that horizontal gene transfer played an important role in the acquisition of genes involved in iron and manganese homeostasis, in multidrug resistance, in bacterium-host interaction, and in virulence. Metabolic analyses of the genome sequence indicated that the "lipophilic" phenotype of C. jeikeium most likely originates from the absence of fatty acid synthase and thus represents a fatty acid auxotrophy. Accordingly, both the complete gene repertoire and the deduced lifestyle of C. jeikeium K411 largely reflect the strict dependence of growth on the presence of exogenous fatty acids. The predicted virulence factors of C. jeikeium K411 are apparently involved in ensuring the availability of exogenous fatty acids by damaging the host tissue.
ESTHER : Tauch_2005_J.Bacteriol_187_4671
PubMedSearch : Tauch_2005_J.Bacteriol_187_4671
PubMedID: 15968079
Gene_locus related to this paper: corjk-q4jsq8 , corjk-q4jst9 , corjk-q4jt28 , corjk-q4jt69 , corjk-q4jta2 , corjk-q4jth4 , corjk-q4jti5 , corjk-q4ju26 , corjk-q4jug4 , corjk-q4juz4 , corjk-q4jv41 , corjk-q4jvr2 , corjk-q4jwi5 , corjk-q4jwq5 , corjk-q4jwu9 , corjk-q4jx57 , corjk-q4jx58 , corjk-q4jxf4 , corjk-q4jxg1 , corjk-q4jxi0 , corjk-q4jxk5 , corjk-q4jxw6 , corjk-q4jxx7 , corjk-q4jy17 , corjk-q4jy19 , corjk-q4jy21 , corjk-q4jy22 , corjk-q4jyd0 , corjk-q4jyf2