Hehlert_2019_J.Lipid.Res_60_1365

Reference

Title : The alpha\/beta-hydrolase domain-containing 4- and 5-related phospholipase Pummelig controls energy storage in Drosophila - Hehlert_2019_J.Lipid.Res_60_1365
Author(s) : Hehlert P , Hofferek V , Heier C , Eichmann TO , Riedel D , Rosenberg J , Takacs A , Nagy HM , Oberer M , Zimmermann R , Kuhnlein RP
Ref : J Lipid Res , 60 :1365 , 2019
Abstract :

Triglycerides (TGs) are the main energy storage form that accommodates changing organismal energy demands. In Drosophila melanogaster, the TG lipase Brummer is centrally important for body fat mobilization. Its gene brummer (bmm) encodes the ortholog of mammalian adipose TG lipase, which becomes activated by alpha/beta-hydrolase domain-containing 5 (ABHD5/CGI-58), one member of the paralogous gene pair, alpha/beta-hydrolase domain-containing 4 (ABHD4) and ABHD5 In Drosophila, the pummelig (puml) gene encodes the single sequence-related protein to mammalian ABHD4/ABHD5 with unknown function. We generated puml deletion mutant flies, that were short-lived as a result of lipid metabolism changes, stored excess body fat at the expense of glycogen, and exhibited ectopic fat storage with altered TG FA profile in the fly kidneys, called Malpighian tubules. TG accumulation in puml mutants was not associated with increased food intake but with elevated lipogenesis; starvation-induced lipid mobilization remained functional. Despite its structural similarity to mammalian ABHD5, Puml did not stimulate TG lipase activity of Bmm in vitro. Rather, Puml acted as a phospholipase that localized on lipid droplets, mitochondria, and peroxisomes. Together, these results show that the ABHD4/5 family member Puml is a versatile phospholipase that regulates Drosophila body fat storage and energy metabolism.

PubMedSearch : Hehlert_2019_J.Lipid.Res_60_1365
PubMedID: 31164391
Gene_locus related to this paper: drome-CG1882

Related information

Gene_locus drome-CG1882
Family drome-CG1882    CGI-58_ABHD5_ABHD4

Citations formats

Hehlert P, Hofferek V, Heier C, Eichmann TO, Riedel D, Rosenberg J, Takacs A, Nagy HM, Oberer M, Zimmermann R, Kuhnlein RP (2019)
The alpha\/beta-hydrolase domain-containing 4- and 5-related phospholipase Pummelig controls energy storage in Drosophila
J Lipid Res 60 :1365

Hehlert P, Hofferek V, Heier C, Eichmann TO, Riedel D, Rosenberg J, Takacs A, Nagy HM, Oberer M, Zimmermann R, Kuhnlein RP (2019)
J Lipid Res 60 :1365