Kawai_2014_Front.Microbiol_5_80

Reference

Title : High frequency of phylogenetically diverse reductive dehalogenase-homologous genes in deep subseafloor sedimentary metagenomes - Kawai_2014_Front.Microbiol_5_80
Author(s) : Kawai M , Futagami T , Toyoda A , Takaki Y , Nishi S , Hori S , Arai W , Tsubouchi T , Morono Y , Uchiyama I , Ito T , Fujiyama A , Inagaki F , Takami H
Ref : Front Microbiol , 5 :80 , 2014
Abstract :

Marine subsurface sediments on the Pacific margin harbor diverse microbial communities even at depths of several hundreds meters below the seafloor (mbsf) or more. Previous PCR-based molecular analysis showed the presence of diverse reductive dehalogenase gene (rdhA) homologs in marine subsurface sediment, suggesting that anaerobic respiration of organohalides is one of the possible energy-yielding pathways in the organic-rich sedimentary habitat. However, primer-independent molecular characterization of rdhA has remained to be demonstrated. Here, we studied the diversity and frequency of rdhA homologs by metagenomic analysis of five different depth horizons (0.8, 5.1, 18.6, 48.5, and 107.0 mbsf) at Site C9001 off the Shimokita Peninsula of Japan. From all metagenomic pools, remarkably diverse rdhA-homologous sequences, some of which are affiliated with novel clusters, were observed with high frequency. As a comparison, we also examined frequency of dissimilatory sulfite reductase genes (dsrAB), key functional genes for microbial sulfate reduction. The dsrAB were also widely observed in the metagenomic pools whereas the frequency of dsrAB genes was generally smaller than that of rdhA-homologous genes. The phylogenetic composition of rdhA-homologous genes was similar among the five depth horizons. Our metagenomic data revealed that subseafloor rdhA homologs are more diverse than previously identified from PCR-based molecular studies. Spatial distribution of similar rdhA homologs across wide depositional ages indicates that the heterotrophic metabolic processes mediated by the genes can be ecologically important, functioning in the organic-rich subseafloor sedimentary biosphere.

PubMedSearch : Kawai_2014_Front.Microbiol_5_80
PubMedID: 24624126
Gene_locus related to this paper: 9zzzz-x0wci8 , 9zzzz-x1k9j7 , 9zzzz-x0se68 , 9zzzz-x0tpm6 , 9zzzz-x0tv89 , 9zzzz-x0ws69 , 9zzzz-x1tg33 , 9zzzz-x1anx0 , 9zzzz-x1m8t8 , 9zzzz-x1a5i7 , 9zzzz-x1k2t2 , 9zzzz-x0uzq5

Related information

Gene_locus 9zzzz-x0wci8    9zzzz-x1k9j7    9zzzz-x0se68    9zzzz-x0tpm6    9zzzz-x0tv89    9zzzz-x0ws69    9zzzz-x1tg33    9zzzz-x1anx0    9zzzz-x1m8t8    9zzzz-x1a5i7    9zzzz-x1k2t2    9zzzz-x0uzq5
Gene_locus_frgt 9zzzz-x0wci8    9zzzz-x1k9j7    9zzzz-x0se68    9zzzz-x0tpm6    9zzzz-x0tv89    9zzzz-x0ws69    9zzzz-x1tg33    9zzzz-x1anx0    9zzzz-x1m8t8    9zzzz-x1a5i7    9zzzz-x1k2t2    9zzzz-x0uzq5    9zzzz-x0t7r8    9zzzz-x1l3v4    9zzzz-x1bgp3    9zzzz-x0rhn1    9zzzz-x1rwb3    9zzzz-x1a128    9zzzz-x1bwr3    9zzzz-x0uih1    9zzzz-x0ver2    9zzzz-x1b9j0    9zzzz-x1v656    9zzzz-x0rwx8    9zzzz-x1dvw4    9zzzz-x0wvp0    9zzzz-x1ad31    9zzzz-x1c425    9zzzz-x0yhm4    9zzzz-x1e7g2    9zzzz-x1h410    9zzzz-x1pvn8    9zzzz-x1d8r1    9zzzz-x1vzn0    9zzzz-x1q4q7    9zzzz-x1ic82    9zzzz-x1cmp9    9zzzz-x1peq7    9zzzz-x1c9l8    9zzzz-x1a444    9zzzz-x1b956    9zzzz-x1sbt3    9zzzz-x0v2j7    9zzzz-x0yb30    9zzzz-x0zlq5    9zzzz-x0rle7    9zzzz-x0u6y5    9zzzz-x0vt21    9zzzz-x1czm7    9zzzz-x1ez80    9zzzz-x1qce0

Citations formats

Kawai M, Futagami T, Toyoda A, Takaki Y, Nishi S, Hori S, Arai W, Tsubouchi T, Morono Y, Uchiyama I, Ito T, Fujiyama A, Inagaki F, Takami H (2014)
High frequency of phylogenetically diverse reductive dehalogenase-homologous genes in deep subseafloor sedimentary metagenomes
Front Microbiol 5 :80

Kawai M, Futagami T, Toyoda A, Takaki Y, Nishi S, Hori S, Arai W, Tsubouchi T, Morono Y, Uchiyama I, Ito T, Fujiyama A, Inagaki F, Takami H (2014)
Front Microbiol 5 :80