Nishi S

References (21)

Title : Clinical value of serum cholinesterase levels in Nephrotic syndrome: an observational study - Goto_2022_BMC.Nephrol_23_128
Author(s) : Goto K , Kono K , Fujii H , Goto S , Nishi S
Ref : BMC Nephrol , 23 :128 , 2022
Abstract : BACKGROUND: Nephrotic syndrome (NS) results in massive proteinuria and hypoalbuminemia, which are responsible for a compensatory increase in protein synthesis in the liver. Serum cholinesterase (ChE) also increases in NS. However, its clinical value is not fully elucidated. METHODS: In this study, 184 patients with NS who underwent kidney biopsy were included. The patients were divided into two groups according to serum ChE levels, as follows: hypercholinesterasemia (HC) and non-hypercholinesterasemia (NHC) groups. The clinical factors were compared between the two groups. RESULTS: The HC group had significantly more severe proteinuria and higher prevalence of high selective proteinuria than the NHC group. Furthermore, the prevalence of minimal change nephrotic syndrome (MCNS) was significantly higher in the HC group than that in the NHC group. Multivariate analysis revealed that the severity of proteinuria and MCNS were significantly associated with HC. CONCLUSION: In this study, HC in NS was associated with the severity of proteinuria and MCNS, and could help clinicians predict the histological diagnosis of NS.
ESTHER : Goto_2022_BMC.Nephrol_23_128
PubMedSearch : Goto_2022_BMC.Nephrol_23_128
PubMedID: 35366840

Title : Complete genome sequence and expression profile of the commercial lytic enzyme producer Lysobacter enzymogenes M497-1 - Takami_2017_DNA.Res_24_169
Author(s) : Takami H , Toyoda A , Uchiyama I , Itoh T , Takaki Y , Arai W , Nishi S , Kawai M , Shin-Ya K , Ikeda H
Ref : DNA Research , 24 :169 , 2017
Abstract : Lysobacter enzymogenes M497-1 is a producer of commercialized achromopeptidase and is expected to harbour genes encoding various other antimicrobial enzymes. Here, we present the complete sequence of the genome of M497-1 and the expression profiles of the genes for various antimicrobial enzymes. Of the 117 peptidase-encoding genes found in the 6.1-Mb genome of M497-1, 15 genes (aside from the gene encoding the achromopeptidase) were expressed at a level higher than that of the average ribosomal protein genes in the 24-h culture. Thus, the strain was found more valuable than hitherto considered. In addition, M497-1 harbours 98 genes involved in the biosynthesis of various natural products, 16 of which are M497-1-specific across 4 Lysobacter species. A gene cluster starting at LEN_2603 through LEN_2673 among the 98 genes closely resembled the lysobactin biosynthesis gene cluster of Lysobacter sp. ATCC 53042. It is likely that M497-1 may produce lysobactin or related antibacterial compounds. Furthermore, comparative genomic analysis of M497-1 and four other Lysobacter species revealed that their core genome structure comprises 3,737 orthologous groups. Our findings are expected to advance further biotechnological application of Lysobacter spp. as a promising source of natural bioactive compounds.
ESTHER : Takami_2017_DNA.Res_24_169
PubMedSearch : Takami_2017_DNA.Res_24_169
PubMedID: 28065880
Gene_locus related to this paper: lysen-a0a1j1ebl3 , lysen-a0a1j1e5b0 , lysen-a0a0s2dfs0

Title : Draft Genome Sequence of Novosphingobium sp. Strain MBES04, Isolated from Sunken Wood from Suruga Bay, Japan - Ohta_2015_Genome.Announc_3_e01373
Author(s) : Ohta Y , Nishi S , Kobayashi K , Tsubouchi T , Iida K , Tanizaki A , Kurosawa K , Adachi A , Nishihara M , Sato R , Hasegawa R , Hatada Y
Ref : Genome Announc , 3 : , 2015
Abstract : This report describes the draft genome sequence of Novosphingobium sp. strain MBES04, isolated from sunken wood from Suruga Bay, Japan, which is capable of degrading a wide range of lignin-related aromatic monomers. The draft genome sequence contains 5,361,448 bp, with a G+C content of 65.4%.
ESTHER : Ohta_2015_Genome.Announc_3_e01373
PubMedSearch : Ohta_2015_Genome.Announc_3_e01373
PubMedID: 25593249
Gene_locus related to this paper: 9sphn-a0a0s6wud3 , 9sphn-a0a0s6wy59 , 9sphn-a0a0s6wym3 , 9sphn-a0a0s6wzq4 , 9sphn-a0a0s6x1d8 , 9sphn-a0a0s6x4h8

Title : High frequency of phylogenetically diverse reductive dehalogenase-homologous genes in deep subseafloor sedimentary metagenomes - Kawai_2014_Front.Microbiol_5_80
Author(s) : Kawai M , Futagami T , Toyoda A , Takaki Y , Nishi S , Hori S , Arai W , Tsubouchi T , Morono Y , Uchiyama I , Ito T , Fujiyama A , Inagaki F , Takami H
Ref : Front Microbiol , 5 :80 , 2014
Abstract : Marine subsurface sediments on the Pacific margin harbor diverse microbial communities even at depths of several hundreds meters below the seafloor (mbsf) or more. Previous PCR-based molecular analysis showed the presence of diverse reductive dehalogenase gene (rdhA) homologs in marine subsurface sediment, suggesting that anaerobic respiration of organohalides is one of the possible energy-yielding pathways in the organic-rich sedimentary habitat. However, primer-independent molecular characterization of rdhA has remained to be demonstrated. Here, we studied the diversity and frequency of rdhA homologs by metagenomic analysis of five different depth horizons (0.8, 5.1, 18.6, 48.5, and 107.0 mbsf) at Site C9001 off the Shimokita Peninsula of Japan. From all metagenomic pools, remarkably diverse rdhA-homologous sequences, some of which are affiliated with novel clusters, were observed with high frequency. As a comparison, we also examined frequency of dissimilatory sulfite reductase genes (dsrAB), key functional genes for microbial sulfate reduction. The dsrAB were also widely observed in the metagenomic pools whereas the frequency of dsrAB genes was generally smaller than that of rdhA-homologous genes. The phylogenetic composition of rdhA-homologous genes was similar among the five depth horizons. Our metagenomic data revealed that subseafloor rdhA homologs are more diverse than previously identified from PCR-based molecular studies. Spatial distribution of similar rdhA homologs across wide depositional ages indicates that the heterotrophic metabolic processes mediated by the genes can be ecologically important, functioning in the organic-rich subseafloor sedimentary biosphere.
ESTHER : Kawai_2014_Front.Microbiol_5_80
PubMedSearch : Kawai_2014_Front.Microbiol_5_80
PubMedID: 24624126
Gene_locus related to this paper: 9zzzz-x0wci8 , 9zzzz-x1k9j7 , 9zzzz-x0se68 , 9zzzz-x0tpm6 , 9zzzz-x0tv89 , 9zzzz-x0ws69 , 9zzzz-x1tg33 , 9zzzz-x1anx0 , 9zzzz-x1m8t8 , 9zzzz-x1a5i7 , 9zzzz-x1k2t2 , 9zzzz-x0uzq5

Title : Draft Genome Sequence of the Dimorphic Prosthecate Bacterium Brevundimonas abyssalis TAR-001T - Tsubouchi_2013_Genome.Announc_1_e00826
Author(s) : Tsubouchi T , Nishi S , Usui K , Shimane Y , Takaki Y , Maruyama T , Hatada Y
Ref : Genome Announc , 1 :e00826 , 2013
Abstract : We report the 3.0-Mb draft genome sequence of Brevundimonas abyssalis strain TAR-001(T), isolated from deep-sea floor sediment. The draft genome sequence of strain TAR-001(T) consists of 2,979,700 bp in 128 contigs, with a G+C content of 68.2%, 2,946 potential coding sequences (CDS), 3 rRNAs, and 41 tRNAs.
ESTHER : Tsubouchi_2013_Genome.Announc_1_e00826
PubMedSearch : Tsubouchi_2013_Genome.Announc_1_e00826
PubMedID: 24136847
Gene_locus related to this paper: 9caul-u2zck3 , 9caul-u2zbn9 , 9caul-u3ap46 , 9caul-u3auy7

Title : A deeply branching thermophilic bacterium with an ancient acetyl-CoA pathway dominates a subsurface ecosystem - Takami_2012_PLoS.One_7_e30559
Author(s) : Takami H , Noguchi H , Takaki Y , Uchiyama I , Toyoda A , Nishi S , Chee GJ , Arai W , Nunoura T , Itoh T , Hattori M , Takai K
Ref : PLoS ONE , 7 :e30559 , 2012
Abstract : A nearly complete genome sequence of Candidatus 'Acetothermum autotrophicum', a presently uncultivated bacterium in candidate division OP1, was revealed by metagenomic analysis of a subsurface thermophilic microbial mat community. Phylogenetic analysis based on the concatenated sequences of proteins common among 367 prokaryotes suggests that Ca. 'A. autotrophicum' is one of the earliest diverging bacterial lineages. It possesses a folate-dependent Wood-Ljungdahl (acetyl-CoA) pathway of CO(2) fixation, is predicted to have an acetogenic lifestyle, and possesses the newly discovered archaeal-autotrophic type of bifunctional fructose 1,6-bisphosphate aldolase/phosphatase. A phylogenetic analysis of the core gene cluster of the acethyl-CoA pathway, shared by acetogens, methanogens, some sulfur- and iron-reducers and dechlorinators, supports the hypothesis that the core gene cluster of Ca. 'A. autotrophicum' is a particularly ancient bacterial pathway. The habitat, physiology and phylogenetic position of Ca. 'A. autotrophicum' support the view that the first bacterial and archaeal lineages were H(2)-dependent acetogens and methanogenes living in hydrothermal environments.
ESTHER : Takami_2012_PLoS.One_7_e30559
PubMedSearch : Takami_2012_PLoS.One_7_e30559
PubMedID: 22303444
Gene_locus related to this paper: 9bact-h5srl9 , 9chlr-h5sfm8

Title : Insights into the evolution of Archaea and eukaryotic protein modifier systems revealed by the genome of a novel archaeal group - Nunoura_2011_Nucleic.Acids.Res_39_3204
Author(s) : Nunoura T , Takaki Y , Kakuta J , Nishi S , Sugahara J , Kazama H , Chee GJ , Hattori M , Kanai A , Atomi H , Takai K , Takami H
Ref : Nucleic Acids Research , 39 :3204 , 2011
Abstract : The domain Archaea has historically been divided into two phyla, the Crenarchaeota and Euryarchaeota. Although regarded as members of the Crenarchaeota based on small subunit rRNA phylogeny, environmental genomics and efforts for cultivation have recently revealed two novel phyla/divisions in the Archaea; the 'Thaumarchaeota' and 'Korarchaeota'. Here, we show the genome sequence of Candidatus 'Caldiarchaeum subterraneum' that represents an uncultivated crenarchaeotic group. A composite genome was reconstructed from a metagenomic library previously prepared from a microbial mat at a geothermal water stream of a sub-surface gold mine. The genome was found to be clearly distinct from those of the known phyla/divisions, Crenarchaeota (hyperthermophiles), Euryarchaeota, Thaumarchaeota and Korarchaeota. The unique traits suggest that this crenarchaeotic group can be considered as a novel archaeal phylum/division. Moreover, C. subterraneum harbors an ubiquitin-like protein modifier system consisting of Ub, E1, E2 and small Zn RING finger family protein with structural motifs specific to eukaryotic system proteins, a system clearly distinct from the prokaryote-type system recently identified in Haloferax and Mycobacterium. The presence of such a eukaryote-type system is unprecedented in prokaryotes, and indicates that a prototype of the eukaryotic protein modifier system is present in the Archaea.
ESTHER : Nunoura_2011_Nucleic.Acids.Res_39_3204
PubMedSearch : Nunoura_2011_Nucleic.Acids.Res_39_3204
PubMedID: 21169198
Gene_locus related to this paper: 9arch-e6n6u9

Title : Reduced genome of the thioautotrophic intracellular symbiont in a deep-sea clam, Calyptogena okutanii - Kuwahara_2007_Curr.Biol_17_881
Author(s) : Kuwahara H , Yoshida T , Takaki Y , Shimamura S , Nishi S , Harada M , Matsuyama K , Takishita K , Kawato M , Uematsu K , Fujiwara Y , Sato T , Kato C , Kitagawa M , Kato I , Maruyama T
Ref : Current Biology , 17 :881 , 2007
Abstract : Although dense animal communities at hydrothermal vents and cold seeps rely on symbioses with chemoautotrophic bacteria [1, 2], knowledge of the mechanisms underlying these chemosynthetic symbioses is still fragmentary because of the difficulty in culturing the symbionts and the hosts in the laboratory. Deep-sea Calyptogena clams harbor thioautotrophic bacterial symbionts in their gill epithelial cells [1, 2]. They have vestigial digestive tracts and nutritionally depend on their symbionts [3], which are vertically transmitted via eggs [4]. To clarify the symbionts' metabolic roles in the symbiosis and adaptations to intracellular conditions, we present the complete genome sequence of the symbiont of Calyptogena okutanii. The genome is a circular chromosome of 1,022,154 bp with 31.6% guanine + cytosine (G + C) content, and is the smallest reported genome in autotrophic bacteria. It encodes 939 protein-coding genes, including those for thioautotrophy and for the syntheses of almost all amino acids and various cofactors. However, transporters for these substances to the host cell are apparently absent. Genes that are unnecessary for an intracellular lifestyle, as well as some essential genes (e.g., ftsZ for cytokinesis), appear to have been lost from the symbiont genome. Reductive evolution of the genome might be ongoing in the vertically transmitted Calyptogena symbionts.
ESTHER : Kuwahara_2007_Curr.Biol_17_881
PubMedSearch : Kuwahara_2007_Curr.Biol_17_881
PubMedID: 17493812
Gene_locus related to this paper: vesoh-a5cvz2

Title : Genetic and functional properties of uncultivated thermophilic crenarchaeotes from a subsurface gold mine as revealed by analysis of genome fragments - Nunoura_2005_Environ.Microbiol_7_1967
Author(s) : Nunoura T , Hirayama H , Takami H , Oida H , Nishi S , Shimamura S , Suzuki Y , Inagaki F , Takai K , Nealson KH , Horikoshi K
Ref : Environ Microbiol , 7 :1967 , 2005
Abstract : Within a phylum Crenarchaeota, only some members of the hyperthermophilic class Thermoprotei, have been cultivated and characterized. In this study, we have constructed a metagenomic library from a microbial mat formation in a subsurface hot water stream of the Hishikari gold mine, Japan, and sequenced genome fragments of two different phylogroups of uncultivated thermophilic Crenarchaeota: (i) hot water crenarchaeotic group (HWCG) I (41.2 kb), and (ii) HWCG III (49.3 kb). The genome fragment of HWCG I contained a 16S rRNA gene, two tRNA genes and 35 genes encoding proteins but no 23S rRNA gene. Among the genes encoding proteins, several genes for putative aerobic-type carbon monoxide dehydrogenase represented a potential clue with regard to the yet unknown metabolism of HWCG I Archaea. The genome fragment of HWCG III contained a 16S/23S rRNA operon and 44 genes encoding proteins. In the 23S rRNA gene, we detected a homing-endonuclease encoding a group I intron similar to those detected in hyperthermophilic Crenarchaeota and Bacteria, as well as eukaryotic organelles. The reconstructed phylogenetic tree based on the 23S rRNA gene sequence reinforced the intermediate phylogenetic affiliation of HWCG III bridging the hyperthermophilic and non-thermophilic uncultivated Crenarchaeota.
ESTHER : Nunoura_2005_Environ.Microbiol_7_1967
PubMedSearch : Nunoura_2005_Environ.Microbiol_7_1967
PubMedID: 16309394
Gene_locus related to this paper: 9bact-h5srl9 , 9chlr-h5sfm8 , 9arch-e6n6u9

Title : Genomic characterization of thermophilic Geobacillus species isolated from the deepest sea mud of the Mariana Trench - Takami_2004_Extremophiles_8_351
Author(s) : Takami H , Nishi S , Lu J , Shimamura S , Takaki Y
Ref : Extremophiles , 8 :351 , 2004
Abstract : The thermophilic strains HTA426 and HTA462 isolated from the Mariana Trench were identified as Geobacillus kaustophilus and G. stearothermophilus, respectively, based on physiologic and phylogenetic analyses using 16S rDNA sequences and DNA-DNA relatedness. The genome size of HTA426 and HTA462 was estimated at 3.23-3.49 Mb and 3.7-4.49 Mb, respectively. The nucleotide sequences of three independent lambda-phage inserts of G. stearothermophilus HTA462 have been determined. The organization of protein coding sequences (CDSs) in the two lambda-phage inserts was found to differ from that in the contigs corresponding to each lambda insert assembled by the shotgun clones of the G. kaustophilus HTA426 genome, although the CDS organization in another lambda insert is identical to that in the HTA426 genome.
ESTHER : Takami_2004_Extremophiles_8_351
PubMedSearch : Takami_2004_Extremophiles_8_351
PubMedID: 15168170
Gene_locus related to this paper: bac25-mglp

Title : Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus - Takami_2004_Nucleic.Acids.Res_32_6292
Author(s) : Takami H , Takaki Y , Chee GJ , Nishi S , Shimamura S , Suzuki H , Matsui S , Uchiyama I
Ref : Nucleic Acids Research , 32 :6292 , 2004
Abstract : We present herein the first complete genome sequence of a thermophilic Bacillus-related species, Geobacillus kaustophilus HTA426, which is composed of a 3.54 Mb chromosome and a 47.9 kb plasmid, along with a comparative analysis with five other mesophilic bacillar genomes. Upon orthologous grouping of the six bacillar sequenced genomes, it was found that 1257 common orthologous groups composed of 1308 genes (37%) are shared by all the bacilli, whereas 839 genes (24%) in the G.kaustophilus genome were found to be unique to that species. We were able to find the first prokaryotic sperm protamine P1 homolog, polyamine synthase, polyamine ABC transporter and RNA methylase in the 839 unique genes; these may contribute to thermophily by stabilizing the nucleic acids. Contrasting results were obtained from the principal component analysis (PCA) of the amino acid composition and synonymous codon usage for highlighting the thermophilic signature of the G.kaustophilus genome. Only in the PCA of the amino acid composition were the Bacillus-related species located near, but were distinguishable from, the borderline distinguishing thermophiles from mesophiles on the second principal axis. Further analysis revealed some asymmetric amino acid substitutions between the thermophiles and the mesophiles, which are possibly associated with the thermoadaptation of the organism.
ESTHER : Takami_2004_Nucleic.Acids.Res_32_6292
PubMedSearch : Takami_2004_Nucleic.Acids.Res_32_6292
PubMedID: 15576355
Gene_locus related to this paper: bac25-mglp , geoka-q5kva3 , geoka-q5kvf2 , geoka-q5kvx7 , geoka-q5kvz7 , geoka-q5kzv8 , geoka-q5l1a6 , geoka-q5l1d4 , geoka-q5l1n0 , geoka-q5l1u3 , geoka-q5l3h0 , geotn-a4isp0 , geoka-g5eba9

Title : A non-adrenergic, non-cholinergic slow inhibitory post-synaptic potential in neurones of the guinea-pig submucous plexus - Mihara_1987_J.Physiol_390_357
Author(s) : Mihara S , Nishi S , North RA , Surprenant A
Ref : Journal of Physiology , 390 :357 , 1987
Abstract : 1. Intracellular recordings were made from neurones in the submucous plexus of guinea-pig ileum and caecum. The responses to electrical stimulation of fibre strands entering the nodes of the plexus were studied. 2. Stimuli comprising trains of pulses (20 Hz, 1-5 s) produced nicotinic excitatory post-synaptic potentials (fast e.p.s.p.s), an adrenergic inhibitory post-synaptic potential (i.p.s.p.), a slow excitatory post-synaptic potential (slow e.p.s.p.) and a fourth, hitherto unnoticed, slow hyperpolarization which followed the slow e.p.s.p. All these responses were abolished by tetrodotoxin or solutions containing a low calcium concentration. 3. The slow hyperpolarization (slow i.p.s.p.) was examined in the presence of blockers of the nicotinic and adrenergic responses, and in conditions in which the slow e.p.s.p. was prevented by desensitizing concentrations of substance P or vasoactive intestinal polypeptide. The slow i.p.s.p. was unaffected by prazosin (0.1-1 microM), propranolol (0.1-1 microM), atropine (1 microM) or naloxone (1 microM). 4. The amplitude and duration of the slow i.p.s.p. increased with increasing numbers of stimulus pulses; it had an amplitude of 17 mV and a duration of 70 s when evoked by a stimulus of 20 Hz for 3 s. 5. The slow i.p.s.p. was associated with a decrease in the input resistance of the cell. It reversed polarity at -90 mV in 4.7 mM-potassium and the extrapolated reversal potential in 0.47 mM-potassium was -145 mV; these findings indicate that the slow i.p.s.p. results from an increase in membrane potassium conductance. 6. The slow i.p.s.p. could still be recorded from submucous plexus neurones in segments of ileum which had been extrinsically denervated 6-11 days previously.(ABSTRACT TRUNCATED AT 250 WORDS)
ESTHER : Mihara_1987_J.Physiol_390_357
PubMedSearch : Mihara_1987_J.Physiol_390_357
PubMedID: 2450995

Title : Electrophysiology, acetylcholine and acetylcholinesterase of immature spinal ganglia of the rabbit--an experimental study and a review -
Author(s) : Karczmar AG , Nishi S , Minota S , Kindel G
Ref : General Pharmacology , 11 :127 , 1980
PubMedID: 7364196

Title : An analysis of the effect of angiotensin II on mammalian ganglion cells -
Author(s) : Dun NJ , Nishi S , Karczmar AG
Ref : Journal of Pharmacology & Experimental Therapeutics , 204 :669 , 1978
PubMedID: 633071

Title : Alteration in nicotinic and muscarinic responses of rabbit superior cervical ganglion cells after chronic preganglionic denervation -
Author(s) : Dun N , Nishi S , Karczmar AG
Ref : Neuropharmacology , 15 :211 , 1976
PubMedID: 934431

Title : Electrical properties of the membrane of denervated mammalian sympathetic ganglion cells -
Author(s) : Dun N , Nishi S , Karczmar AG
Ref : Neuropharmacology , 15 :219 , 1976
PubMedID: 934432

Title : Primary afferent neurones: the ionic mechanism of GABA-mediated depolarization -
Author(s) : Nishi S , Minota S , Karczmar AG
Ref : Neuropharmacology , 13 :215 , 1974
PubMedID: 4546218

Title : Intracellular recording from the myenteric plexus of the guinea-pig ileum - Nishi_1973_J.Physiol_231_471
Author(s) : Nishi S , North RA
Ref : Journal of Physiology , 231 :471 , 1973
Abstract : 1. Ganglion cells of the myenteric plexus of the guinea-pig ileum have been studied with intracellular micro-electrodes.2. Three types of cell were distinguished. Type 1 cells had a high resistance (58 MOmega) and had properties similar to guinea-pig sympathetic ganglion cells. Type 2 cells were also excitable but had a lower resistance (21 MOmega) and showed accommodation to depolarizing current pulses. Type 3 cells were inexcitable.3. Point stimulation within 150 mum excited neurones either antidromically or orthodromically, sometimes both.4. Antidromic responses had a small all-or-nothing component which was subthreshold for the soma spike. Two or more such components sometimes occurred, and were probably due to stimulation of two or more cell processes.5. Excitatory post-synaptic potentials (e.p.s.p.s) were blocked by hexamethonium (400 muM). They progressively declined in amplitude when elicited at frequencies of 0.05 Hz or more, and this is discussed in relation to studies on acetylcholine (ACh) output.6. Many cells often showed a slow after-hyperpolarization following a direct or antidromic spike. Its mechanism and significance are discussed.7. Spontaneous e.p.s.p.s and spikes were occasionally seen.8. Intracellular injection of a fluorescent dye reveals that the neurones have one to seven processes, which usually arise from the poles of the oval soma.
ESTHER : Nishi_1973_J.Physiol_231_471
PubMedSearch : Nishi_1973_J.Physiol_231_471
PubMedID: 4783093

Title : Presynaptic action of noradrenaline in the myenteric plexus -
Author(s) : Nishi S , North RA
Ref : Journal of Physiology , 231 :29P , 1973
PubMedID: 4715358

Title : The types and sites of cholinergic receptors -
Author(s) : Karczmar AG , Nishi S
Ref : Advances in Cytopharmacology , 1 :301 , 1971
PubMedID: 4356764

Title : Release of acetylcholine from sympathetic preganglionic nerve terminale -
Author(s) : Nishi S , Soeda H , Koketsu K
Ref : Journal of Neurophysiology , 30 :114 , 1967