| Title : Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel - Koudelakova_2013_Angew.Chem.Int.Ed.Engl_52_1959 |
| Author(s) : Koudelakova T , Chaloupkova R , Brezovsky J , Prokop Z , Sebestova E , Hesseler M , Khabiri M , Plevaka M , Kulik D , Kuta Smatanova I , Rezacova P , Ettrich R , Bornscheuer UT , Damborsky J |
| Ref : Angew Chem Int Ed Engl , 52 :1959 , 2013 |
|
Abstract :
Mutations targeting as few as four residues lining the access tunnel extended the half-life of an enzyme in 40% dimethyl sulfoxide from minutes to weeks and increased its melting temperature by 190C. Protein crystallography and molecular dynamics revealed that the tunnel residue packing is a key determinant of protein stability and the active-site accessibility for cosolvent molecules (red dots). |
| PubMedSearch : Koudelakova_2013_Angew.Chem.Int.Ed.Engl_52_1959 |
| PubMedID: 23303607 |
| Gene_locus related to this paper: rhoso-halo1 |
| Inhibitor | Benzoic-acid |
| Gene_locus | rhoso-halo1 |
| Family | Haloalkane_dehalogenase-HLD2 |
| Structure | 4F5Z 4F60 |
Koudelakova T, Chaloupkova R, Brezovsky J, Prokop Z, Sebestova E, Hesseler M, Khabiri M, Plevaka M, Kulik D, Kuta Smatanova I, Rezacova P, Ettrich R, Bornscheuer UT, Damborsky J (2013)
Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel
Angew Chem Int Ed Engl
52 :1959
Koudelakova T, Chaloupkova R, Brezovsky J, Prokop Z, Sebestova E, Hesseler M, Khabiri M, Plevaka M, Kulik D, Kuta Smatanova I, Rezacova P, Ettrich R, Bornscheuer UT, Damborsky J (2013)
Angew Chem Int Ed Engl
52 :1959