Lin_2024_Commun.Biol_7_781

Reference

Title : The global distribution of the macrolide esterase EstX from the alpha\/beta hydrolase superfamily - Lin_2024_Commun.Biol_7_781
Author(s) : Lin J , Lv H , Wang T , Tao H , Zhong Y , Zhou Y , Tang Y , Xie F , Zhuang G , Xu C , Chu Y , Wang X , Yang Y , Song T
Ref : Commun Biol , 7 :781 , 2024
Abstract :

Macrolide antibiotics, pivotal in clinical therapeutics, are confronting resistance challenges mediated by enzymes like macrolide esterases, which are classified into Ere-type and the less studied Est-type. In this study, we provide the biochemical confirmation of EstX, an Est-type macrolide esterase that initially identified as unknown protein in the 1980s. EstX is capable of hydrolyzing four 16-membered ring macrolides, encompassing both veterinary (tylosin, tidipirosin, and tilmicosin) and human-use (leucomycin A5) antibiotics. It uses typical catalytic triad (Asp233-His261-Ser102) from alpha/beta hydrolase superfamily for ester bond hydrolysis. Further genomic context analysis suggests that the dissemination of estX is likely facilitated by mobile genetic elements such as integrons and transposons. The global distribution study indicates that bacteria harboring the estX gene, predominantly pathogenic species like Escherichia coli, Salmonella enterica, and Klebsiella pneumoniae, are prevalent in 74 countries across 6 continents. Additionally, the emergence timeline of the estX gene suggests its proliferation may be linked to the overuse of macrolide antibiotics. The widespread prevalence and dissemination of Est-type macrolide esterase highlight an urgent need for enhanced monitoring and in-depth research, underlining its significance as an escalating public health issue.

PubMedSearch : Lin_2024_Commun.Biol_7_781
PubMedID: 38944651
Gene_locus related to this paper: ecoli-estX

Related information

Citations formats

Lin J, Lv H, Wang T, Tao H, Zhong Y, Zhou Y, Tang Y, Xie F, Zhuang G, Xu C, Chu Y, Wang X, Yang Y, Song T (2024)
The global distribution of the macrolide esterase EstX from the alpha\/beta hydrolase superfamily
Commun Biol 7 :781

Lin J, Lv H, Wang T, Tao H, Zhong Y, Zhou Y, Tang Y, Xie F, Zhuang G, Xu C, Chu Y, Wang X, Yang Y, Song T (2024)
Commun Biol 7 :781