Qi_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_376

Reference

Title : The structure of a complex of the lactonohydrolase zearalenone hydrolase with the hydrolysis product of zearalenone at 1.60 A resolution - Qi_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_376
Author(s) : Qi Q , Yang WJ , Zhou HJ , Ming DM , Sun KL , Xu TY , Hu XJ , Lv H
Ref : Acta Crystallographica F Struct Biol Commun , 73 :376 , 2017
Abstract :

Zearalenone hydrolase (ZHD) is an alpha/beta-hydrolase that detoxifies and degrades the lactone zearalenone (ZEN), a naturally occurring oestrogenic mycotoxin that contaminates crops. Several apoenzyme and enzyme-substrate complex structures have been reported in the resolution range 2.4-2.6 A. However, the properties and mechanism of this enzyme are not yet fully understood. Here, a 1.60 A resolution structure of a ZHD-product complex is reported which was determined from a C-terminally His6-tagged ZHD crystal soaked with 2 mM ZEN for 30 min. It shows that after the lactone-bond cleavage, the phenol-ring region moves closer to residues Leu132, Tyr187 and Pro188, while the lactone-ring region barely moves. Comparisons of the ZHD-substrate and ZHD-product structures show that the hydrophilic interactions change, especially Trp183 N1, which shifts from contacting O2 to O12', suggesting that Trp183 is responsible for the unidirectional translational movement of the phenol ring. This structure provides information on the final stage of the catalytic mechanism of zearalenone hydrolysis.

PubMedSearch : Qi_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_376
PubMedID: 28695844
Gene_locus related to this paper: biooc-ZHD101

Related information

Inhibitor ZFR
Substrate Zearalenone
Gene_locus biooc-ZHD101
Family Zearalenone-hydrolase
Structure 5C8Z    5XMW

Citations formats

Qi Q, Yang WJ, Zhou HJ, Ming DM, Sun KL, Xu TY, Hu XJ, Lv H (2017)
The structure of a complex of the lactonohydrolase zearalenone hydrolase with the hydrolysis product of zearalenone at 1.60 A resolution
Acta Crystallographica F Struct Biol Commun 73 :376

Qi Q, Yang WJ, Zhou HJ, Ming DM, Sun KL, Xu TY, Hu XJ, Lv H (2017)
Acta Crystallographica F Struct Biol Commun 73 :376