Zearalenone-hydrolase

Relationship

Family: Zearalenone-hydrolase

Block: X

Parent Family: No family

Comment

The mycotoxin zearalenone has been contaminating maize and other grains. Zearalenone is a macrolide comprising a fourteen-membered lactone fused to 1,3-dihydroxybenzene, a member of resorcinols; a potent estrogenic metabolite produced by some Giberella species. It has a role as a fungal metabolite and a mycoestrogen. It can be hydrolyzed and inactivated by the lactonase ZHD. Zearalenone differs from other quorum-sensing lactones Fruhauf et al. 2024 describe another family which differs from this previously isolated family. The second family is Zearalenone-hydrolase-fam2 family

Database

Interpro : No interpro

PIRSF : No PIRSF

Pdoc : No Pdoc

Pfam : No Pfam

Prints : No Print

EC Number : No EC Number

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
8JLV Structure of Exophiala aquamarina CBS 119918. AB hydrolase-1 zearalenone lactone hydrolase 9euro-a0a072pad1
6L7M Characterization and structural analysis of a thermostable zearalenone-degrading enzyme 9pezi-a0a4q4ube4
5XWZ Crystal structure of a lactonase from Cladophialophora bantiana xylba-a0a0d2h023
5XO6 Crystal structure of a novel ZEN lactonase mutant S105A from Rhinocladiella mackenzie (fungus) 9euro-a0a0d2ilk1
5XO7 Crystal structure of a novel ZEN lactonase mutant S105A from Rhinocladiella mackenzie (fungus) in complex with alpha-ZOL 9euro-a0a0d2ilk1
5XO8 Crystal structure of a novzl ZEN lactonase mutant S105A from Rhinocladiella mackenzie (fungus) in complex with Zearalenone 9euro-a0a0d2ilk1
5Z5J Crystal structure of a novel ZEN lactonase double mutant S105A\/Y160A from Rhinocladiella mackenzie (fungus) 9euro-a0a0d2ilk1
5Z7J Crystal structure of a novel ZEN lactonase double mutant S105A\/Y160A from Rhinocladiella mackenzie (fungus) in complex with alpha-ZOL 9euro-a0a0d2ilk1
5Z97 Crystal structure of a novel ZEN lactonase double mutant S105A\/Y160A from Rhinocladiella mackenzie (fungus) in complex with ZEN 9euro-a0a0d2ilk1
5C7Y Clonostachys rosea (fungus) ZHD-Intermediate complex after ZHD crystal soaking in ZEN for 9min biooc-ZHD101
5C81 Clonostachys rosea (fungus) ZHD-Intermediate complex after ZHD crystal soaking in ZEN for 12min biooc-ZHD101
5C8Z Clonostachys rosea (fungus) ZHD-ZGR complex after ZHD crystal soaking in ZEN for 30min || Clonostachys rosea (fungus) Lactonohydrolase zearalenone hydrolase ZHD-ZGR complex after ZHD crystal soaking in ZEN (mycoestrogen-detoxifying lactonase ZEN lactonase) for 30min biooc-ZHD101
5C8X Clonostachys rosea (fungus) ZHD-Intermediate complex after ZHD crystal soaking in ZEN for 20min biooc-ZHD101
5XMW Clonostachys rosea (fungus) Lactonohydrolase zearalenone hydrolase Selenomethionine-derivated ZHD biooc-ZHD101
5IE4 Crystal structure of a Clonostachys rosea (fungus) lactonase mutant in complex with substrate a biooc-ZHD101
5IE5 Crystal structure of a Clonostachys rosea (fungus) lactonase double mutant in complex with substrate a biooc-ZHD101
5IE6 Crystal structure of a Clonostachys rosea (fungus) lactonase mutant in complex with substrate b biooc-ZHD101
5IE7 Crystal structure of a Clonostachys rosea lactonase double mutant in complex with substrate b biooc-ZHD101
3WZL Crystal structure of the mycoestrogen-detoxifying lactonase ZHD (ZEN lactonase) from Clonostachys rosea biooc-ZHD101
3WZM Crystal structure of the mycoestrogen-detoxifying lactonase ZHD (ZEN lactonase mutant complex) from Clonostachys rosea biooc-ZHD101
6JQZ Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/H242A complex with ZEN from Clonostachys rosea biooc-ZHD101
6JR2 Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/H242A complex with alpha-ZOL from Clonostachys rosea biooc-ZHD101
6JR5 Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/H242A complex with beta-ZOL from Clonostachys rosea biooc-ZHD101
6JR9 Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/W183F complex with ZEN from Clonostachys rosea biooc-ZHD101
6JRA Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/W183F complex with alpha-ZOL from Clonostachys rosea biooc-ZHD101
6JRB Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/W183F complex with beta-ZOL from Clonostachys rosea biooc-ZHD101
6JRC Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD complex with alpha-ZOL from Clonostachys rosea biooc-ZHD101
6JRD Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD complex with beta-ZOL from Clonostachys rosea biooc-ZHD101

Gene_locus Name Species
9euro-a0a0d2ilk1 Rhinocladiella mackenziei CBS 650.93 Z518_04590 Zhd518 Mycoestrogen-Detoxifying Lactonase Rhinocladiella mackenziei CBS 650.93
xylba-a0a0d2h023 Cladophialophora bantiana zearalenone hydrolase CbZHD Cladophialophora bantiana CBS 173.52
biooc-ZHD101 Bionectria ochroleuca (Gliocladium roseum), Clonostachys rosea f. rosea, zearalenone hydrolase ZHD101 ZEN-JJM Bionectria ochroleuca
9pezi-a0a439d8r8 Xylaria grammica. AB hydrolase-1 domain-containing protein Xylaria grammica
9pezi-a0a4z0yns1 Xylaria hypoxylon. AB hydrolase-1 domain-containing protein Xylaria hypoxylon
9pezi-a0a553hs45 Xylaria flabelliformis. AB hydrolase-1 domain-containing protein Xylaria flabelliformis
9euro-a0a072pad1 Exophiala aquamarina CBS 119918. AB hydrolase-1 domain-containing protein Exophiala aquamarina CBS 119918
eutla-m7tnt1 Eutypa lata (strain UCR-EL1) (Grapevine dieback disease fungus) (Eutypa armeniacae). Putative alpha beta hydrolase fold protein Eutypa lata
9pezi-a0a4q4tp46 Monosporascus sp. GIB2. AB hydrolase-1 domain-containing protein Monosporascus sp. GIB2
9euro-a0a0d2e8j6 Phialophora americana. ZHD607 lactone hydrolase Phialophora americana
9pezi-a0a4q4x369 Monosporascus sp. CRB-9-2. AB hydrolase-1 domain-containing protein Monosporascus sp. CRB-9-2
9euro-a0a0d2edi3 Exophiala xenobiotica. AB hydrolase-1 domain-containing protein Exophiala xenobiotica
9pezi-a0a4v1x8l5 Monosporascus ibericus. AB hydrolase-1 domain-containing protein Monosporascus ibericus
9pezi-a0a4q4ube4 Monosporascus sp. MG133 thermostable zearalenone-degrading enzyme Monosporascus sp. MG133
9euro-w9x961 Cladophialophora psammophila CBS 110553. AB hydrolase-1 domain-containing protein Cladophialophora psammophila CBS 110553
9euro-a0a0d2b6n4 Exophiala spinifera. AB hydrolase-1 domain-containing protein Exophiala spinifera
9euro-a0a178z8l1 Fonsecaea erecta. AB hydrolase-1 domain-containing protein Fonsecaea erecta
9euro-w9vhd2 Cladophialophora psammophila CBS 110553. AB hydrolase-1 domain-containing protein Cladophialophora psammophila CBS 110553
eutla-m7tmb1 Eutypa lata (strain UCR-EL1) (Grapevine dieback disease fungus) (Eutypa armeniacae). Putative alpha beta hydrolase fold protein Eutypa lata
exome-a0a0d2agi6 Exophiala mesophila (Black yeast). AB hydrolase-1 domain-containing protein Exophiala mesophila
rosne-a0a1w2tnw3 Rosellinia necatrix (White root-rot fungus). Putative zearalenone lactonohydrolase Rosellinia necatrix
9euro-a0a0d2jr16 Fonsecaea multimorphosa CBS 102226. AB hydrolase-1 domain-containing protein Fonsecaea multimorphosa CBS 102226
9euro-a0a0d2h1w2 Fonsecaea pedrosoi CBS 271.37. Unplaced genomic scaffold supercont1.5, whole genome shotgun sequence Fonsecaea pedrosoi CBS 271.37
9pezi-a0a507bfx0 Phialemoniopsis curvata. AB hydrolase-1 domain-containing protein Phialemoniopsis curvata
9pezi-a0a1y2ekn6 Pseudomassariella vexata. Zearalenone hydrolase Pseudomassariella vexata
9euro-a0a0n0nqn9 Phialophora attinorum. AB hydrolase-1 domain-containing protein Phialophora attinorum
9euro-a0a0n0nrd5 Phialophora attinorum. AB hydrolase-1 domain-containing protein Phialophora attinorum
9helo-a0a2j6rsu8 Hyaloscypha variabilis F. Alpha\/beta-hydrolase Hyaloscypha variabilis F
9euro-a0a0d1w8k5 Exophiala sideris. alpha\/beta hydrolase (c-term PKS_ER domain)excluded Exophiala sideris
9helo-a0a2j6s3x0 Hyaloscypha variabilis F. Alpha\/beta-hydrolase Hyaloscypha variabilis F
9helo-a0a1l7xua8 Phialocephala subalpina. AB hydrolase-1 domain-containing protein Phialocephala subalpina
9helo-a0a370tt42 Venustampulla echinocandica. AB hydrolase-1 domain-containing protein Venustampulla echinocandica
9helo-a0a2j6q8q8 Pezoloma ericae. Alpha\/beta-hydrolase Pezoloma ericae
9helo-a0a194x7c3 Phialocephala scopiformis. Alpha\/beta-hydrolase Phialocephala scopiformis

Substrate Chemical Nomenclature Proteins
Zearalenone (2E,11S)-15,17-dihydroxy-11-methyl-12-oxabicyclo[12.4.0]octadeca-1(14),2,15,17-tetraene-7,13-dione biooc-ZHD101    9baci-QBK11187    xylba-a0a0d2h023    9actn-ZenA    9noca-a0aa46n777    neucr-B15B24.080    9euro-a0a0d2ilk1
Beta-zearalenol (2E,7S,11S)-7,15,17-trihydroxy-11-methyl-12-oxabicyclo[12.4.0]octadeca-1(18),2,14,16-tetraen-13-one biooc-ZHD101
Alpha-zearalenol (2E,7R,11S)-7,15,17-trihydroxy-11-methyl-12-oxabicyclo[12.4.0]octadeca-1(14),2,15,17-tetraen-13-one biooc-ZHD101

Inhibitor Chemical Nomenclature Proteins
ZGR 2,4-dihydroxy-6-[(E,10S)-10-hydroxy-6-oxoundec-1-enyl]benzoic acid biooc-ZHD101    9actn-ZenA    9noca-a0aa46n777
ZFR (E,10S)-1-(3,5-dihydroxyphenyl)-10-hydroxyundec-1-en-6-one biooc-ZHD101

References (18)

Title : Degradation mechanism for Zearalenone ring-cleavage by Zearalenone hydrolase RmZHD: A QM\/MM study - Zhou_2020_Sci.Total.Environ_709_135897
Author(s) : Zhou J , Zhu L , Chen J , Wang W , Zhang R , Li Y , Zhang Q
Ref : Sci Total Environ , 709 :135897 , 2020
Abstract :
PubMedSearch : Zhou_2020_Sci.Total.Environ_709_135897
PubMedID: 31887512
Gene_locus related to this paper: 9euro-a0a0d2ilk1

Title : The Trp183 is essential in lactonohydrolase ZHD detoxifying zearalenone and zearalenols - Zhou_2020_Biochem.Biophys.Res.Commun_522_986
Author(s) : Zhou H , Li L , Zhan B , Wang S , Li J , Hu XJ
Ref : Biochemical & Biophysical Research Communications , 522 :986 , 2020
Abstract :
PubMedSearch : Zhou_2020_Biochem.Biophys.Res.Commun_522_986
PubMedID: 31810602
Gene_locus related to this paper: biooc-ZHD101

Title : Theoretical Study on Zearalenol Compounds Binding with Wild Type Zearalenone Hydrolase and V153H Mutant - Liu_2018_Int.J.Mol.Sci_19_
Author(s) : Liu Y , Wan Y , Zhu J , Yu Z , Tian X , Han J , Zhang Z , Han W
Ref : Int J Mol Sci , 19 : , 2018
Abstract :
PubMedSearch : Liu_2018_Int.J.Mol.Sci_19_
PubMedID: 30231501
Gene_locus related to this paper: biooc-ZHD101

Title : Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana - Hui_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_515
Author(s) : Hui R , Hu X , Liu W , Zheng Y , Chen Y , Guo RT , Jin J , Chen CC
Ref : Acta Crystallographica F Struct Biol Commun , 73 :515 , 2017
Abstract :
PubMedSearch : Hui_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_515
PubMedID: 28876230
Gene_locus related to this paper: xylba-a0a0d2h023

Title : Esterase activity inspired selection and characterization of zearalenone degrading bacteria Bacillus pumilus ES-21 - Wang_2017_Food.Control_77_57
Author(s) : Wang G , Yu M , Dong F , Shi J , Xu J
Ref : Food Control , 77 :57 , 2017
Abstract :
PubMedSearch : Wang_2017_Food.Control_77_57
PubMedID:

Title : The structure of a complex of the lactonohydrolase zearalenone hydrolase with the hydrolysis product of zearalenone at 1.60 A resolution - Qi_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_376
Author(s) : Qi Q , Yang WJ , Zhou HJ , Ming DM , Sun KL , Xu TY , Hu XJ , Lv H
Ref : Acta Crystallographica F Struct Biol Commun , 73 :376 , 2017
Abstract :
PubMedSearch : Qi_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_376
PubMedID: 28695844
Gene_locus related to this paper: biooc-ZHD101

Title : Isolation and characterisation of enzymatic zearalenone hydrolysis reaction products - Vekiru_2016_World.Mycotoxin.J_9_353
Author(s) : Vekiru E , Fruhauf S , Hametner C , Schatzmayr G , Krska R , Moll WD , Schuhmacher R
Ref : World Mycotoxin Journal , 9 :353 , 2016
Abstract :
PubMedSearch : Vekiru_2016_World.Mycotoxin.J_9_353
PubMedID:
Gene_locus related to this paper: biooc-ZHD101

Title : High-level expression of a ZEN-detoxifying gene by codon optimization and biobrick in Pichia pastoris - Xiang_2016_Microbiol.Res_193_48
Author(s) : Xiang L , Wang Q , Zhou Y , Yin L , Zhang G , Ma Y
Ref : Microbiol Res , 193 :48 , 2016
Abstract :
PubMedSearch : Xiang_2016_Microbiol.Res_193_48
PubMedID: 27825486
Gene_locus related to this paper: biooc-ZHD101

Title : Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea - Peng_2014_RSC.Adv_4_62321
Author(s) : Peng W , Ko TP , Yang YY , Zheng YY , Chen CC , Zhu Z , Huang CH , Zeng YF , Huang JW , Wang AHJ , Liu JR , Guo RT
Ref : RSC Advances , 4 :62321 , 2014
Abstract :
PubMedSearch : Peng_2014_RSC.Adv_4_62321
PubMedID:
Gene_locus related to this paper: biooc-ZHD101

Title : Zearalenone lactonohydrolase activity in Hypocreales and its evolutionary relationships within the epoxide hydrolase subset of a\/b-hydrolases - Popiel_2014_BMC.Microbiol_14_82
Author(s) : Popiel D , Koczyk G , Dawidziuk A , Gromadzka K , Blaszczyk L , Chelkowski J
Ref : BMC Microbiol , 14 :82 , 2014
Abstract :
PubMedSearch : Popiel_2014_BMC.Microbiol_14_82
PubMedID: 24708405
Gene_locus related to this paper: biooc-ZHD101

Title : Zearalenone detoxification by zearalenone hydrolase is important for the antagonistic ability of Clonostachys rosea against mycotoxigenic Fusarium graminearum - Kosawang_2014_Fungal.Biol_118_364
Author(s) : Kosawang C , Karlsson M , Velez H , Rasmussen PH , Collinge DB , Jensen B , Jensen DF
Ref : Fungal Biol , 118 :364 , 2014
Abstract :
PubMedSearch : Kosawang_2014_Fungal.Biol_118_364
PubMedID: 24742831
Gene_locus related to this paper: biooc-ZHD101

Title : Reduced contamination by the Fusarium mycotoxin zearalenone in maize kernels through genetic modification with a detoxification gene - Igawa_2007_Appl.Environ.Microbiol_73_1622
Author(s) : Igawa T , Takahashi-Ando N , Ochiai N , Ohsato S , Shimizu T , Kudo T , Yamaguchi I , Kimura M
Ref : Applied Environmental Microbiology , 73 :1622 , 2007
Abstract :
PubMedSearch : Igawa_2007_Appl.Environ.Microbiol_73_1622
PubMedID: 17209063
Gene_locus related to this paper: biooc-ZHD101

Title : Two different polyketide synthase genes are required for synthesis of zearalenone in Gibberella zeae - Kim_2005_Mol.Microbiol_58_1102
Author(s) : Kim YT , Lee YR , Jin J , Han KH , Kim H , Kim JC , Lee T , Yun SH , Lee YW
Ref : Molecular Microbiology , 58 :1102 , 2005
Abstract :
PubMedSearch : Kim_2005_Mol.Microbiol_58_1102
PubMedID: 16262793
Gene_locus related to this paper: gibze-q2vlj3

Title : A model transgenic cereal plant with detoxification activity for the estrogenic mycotoxin zearalenone - Higa-Nishiyama_2005_Transgenic.Res_14_713
Author(s) : Higa-Nishiyama A , Takahashi-Ando N , Shimizu T , Kudo T , Yamaguchi I , Kimura M
Ref : Transgenic Res , 14 :713 , 2005
Abstract :
PubMedSearch : Higa-Nishiyama_2005_Transgenic.Res_14_713
PubMedID: 16245162
Gene_locus related to this paper: biooc-ZHD101

Title : Efficient decontamination of zearalenone, the mycotoxin of cereal pathogen, by transgenic yeasts through the expression of a synthetic lactonohydrolase gene - Takahashi-Ando_2005_Appl.Microbiol.Biotechnol_67_838
Author(s) : Takahashi-Ando N , Tokai T , Hamamoto H , Yamaguchi I , Kimura M
Ref : Applied Microbiology & Biotechnology , 67 :838 , 2005
Abstract :
PubMedSearch : Takahashi-Ando_2005_Appl.Microbiol.Biotechnol_67_838
PubMedID: 15630583
Gene_locus related to this paper: biooc-ZHD101

Title : Metabolism of zearalenone by genetically modified organisms expressing the detoxification gene from Clonostachys rosea - Takahashi-Ando_2004_Appl.Environ.Microbiol_70_3239
Author(s) : Takahashi-Ando N , Ohsato S , Shibata T , Hamamoto H , Yamaguchi I , Kimura M
Ref : Applied Environmental Microbiology , 70 :3239 , 2004
Abstract :
PubMedSearch : Takahashi-Ando_2004_Appl.Environ.Microbiol_70_3239
PubMedID: 15184117
Gene_locus related to this paper: biooc-ZHD101

Title : Expression in cereal plants of genes that inactivate Fusarium mycotoxins - Higa_2003_Biosci.Biotechnol.Biochem_67_914
Author(s) : Higa A , Kimura M , Mimori K , Ochiai-Fukuda T , Tokai T , Takahashi-Ando N , Nishiuchi T , Igawa T , Fujimura M , Hamamoto H , Usami R , Yamaguchi I
Ref : Biosci Biotechnol Biochem , 67 :914 , 2003
Abstract :
PubMedSearch : Higa_2003_Biosci.Biotechnol.Biochem_67_914
PubMedID: 12784641
Gene_locus related to this paper: biooc-ZHD101

Title : Purification and characterization of a lactonase from Erwinia cypripedii 314B that hydrolyzes ( S)-5-oxo-2-tetrahydrofurancarboxylic acid - Mochizuki_2003_Arch.Microbiol_180_490
Author(s) : Mochizuki K
Ref : Arch Microbiol , 180 :490 , 2003
Abstract :
PubMedSearch : Mochizuki_2003_Arch.Microbiol_180_490
PubMedID: 14576976
Gene_locus related to this paper: biooc-ZHD101