Zearalenone-hydrolase
Relationship
Comment
The mycotoxin zearalenone has been contaminating maize and other grains. Zearalenone is a macrolide comprising a fourteen-membered lactone fused to 1,3-dihydroxybenzene, a member of resorcinols; a potent estrogenic metabolite produced by some Giberella species. It has a role as a fungal metabolite and a mycoestrogen. It can be hydrolyzed and inactivated by the lactonase ZHD. Zearalenone differs from other quorum-sensing lactones Fruhauf et al. 2024 describe another family which differs from this previously isolated family. The second family is Zearalenone-hydrolase-fam2 family
Database
Interpro : No interpro
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : No Pfam
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 8JLV | Structure of Exophiala aquamarina CBS 119918. AB hydrolase-1 zearalenone lactone hydrolase | 9euro-a0a072pad1 |
| 6L7M | Characterization and structural analysis of a thermostable zearalenone-degrading enzyme | 9pezi-a0a4q4ube4 |
| 5XWZ | Crystal structure of a lactonase from Cladophialophora bantiana | xylba-a0a0d2h023 |
| 5XO6 | Crystal structure of a novel ZEN lactonase mutant S105A from Rhinocladiella mackenzie (fungus) | 9euro-a0a0d2ilk1 |
| 5XO7 | Crystal structure of a novel ZEN lactonase mutant S105A from Rhinocladiella mackenzie (fungus) in complex with alpha-ZOL | 9euro-a0a0d2ilk1 |
| 5XO8 | Crystal structure of a novzl ZEN lactonase mutant S105A from Rhinocladiella mackenzie (fungus) in complex with Zearalenone | 9euro-a0a0d2ilk1 |
| 5Z5J | Crystal structure of a novel ZEN lactonase double mutant S105A\/Y160A from Rhinocladiella mackenzie (fungus) | 9euro-a0a0d2ilk1 |
| 5Z7J | Crystal structure of a novel ZEN lactonase double mutant S105A\/Y160A from Rhinocladiella mackenzie (fungus) in complex with alpha-ZOL | 9euro-a0a0d2ilk1 |
| 5Z97 | Crystal structure of a novel ZEN lactonase double mutant S105A\/Y160A from Rhinocladiella mackenzie (fungus) in complex with ZEN | 9euro-a0a0d2ilk1 |
| 5C7Y | Clonostachys rosea (fungus) ZHD-Intermediate complex after ZHD crystal soaking in ZEN for 9min | biooc-ZHD101 |
| 5C81 | Clonostachys rosea (fungus) ZHD-Intermediate complex after ZHD crystal soaking in ZEN for 12min | biooc-ZHD101 |
| 5C8Z | Clonostachys rosea (fungus) ZHD-ZGR complex after ZHD crystal soaking in ZEN for 30min || Clonostachys rosea (fungus) Lactonohydrolase zearalenone hydrolase ZHD-ZGR complex after ZHD crystal soaking in ZEN (mycoestrogen-detoxifying lactonase ZEN lactonase) for 30min | biooc-ZHD101 |
| 5C8X | Clonostachys rosea (fungus) ZHD-Intermediate complex after ZHD crystal soaking in ZEN for 20min | biooc-ZHD101 |
| 5XMW | Clonostachys rosea (fungus) Lactonohydrolase zearalenone hydrolase Selenomethionine-derivated ZHD | biooc-ZHD101 |
| 5IE4 | Crystal structure of a Clonostachys rosea (fungus) lactonase mutant in complex with substrate a | biooc-ZHD101 |
| 5IE5 | Crystal structure of a Clonostachys rosea (fungus) lactonase double mutant in complex with substrate a | biooc-ZHD101 |
| 5IE6 | Crystal structure of a Clonostachys rosea (fungus) lactonase mutant in complex with substrate b | biooc-ZHD101 |
| 5IE7 | Crystal structure of a Clonostachys rosea lactonase double mutant in complex with substrate b | biooc-ZHD101 |
| 3WZL | Crystal structure of the mycoestrogen-detoxifying lactonase ZHD (ZEN lactonase) from Clonostachys rosea | biooc-ZHD101 |
| 3WZM | Crystal structure of the mycoestrogen-detoxifying lactonase ZHD (ZEN lactonase mutant complex) from Clonostachys rosea | biooc-ZHD101 |
| 6JQZ | Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/H242A complex with ZEN from Clonostachys rosea | biooc-ZHD101 |
| 6JR2 | Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/H242A complex with alpha-ZOL from Clonostachys rosea | biooc-ZHD101 |
| 6JR5 | Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/H242A complex with beta-ZOL from Clonostachys rosea | biooc-ZHD101 |
| 6JR9 | Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/W183F complex with ZEN from Clonostachys rosea | biooc-ZHD101 |
| 6JRA | Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/W183F complex with alpha-ZOL from Clonostachys rosea | biooc-ZHD101 |
| 6JRB | Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD\/W183F complex with beta-ZOL from Clonostachys rosea | biooc-ZHD101 |
| 6JRC | Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD complex with alpha-ZOL from Clonostachys rosea | biooc-ZHD101 |
| 6JRD | Crystal structure of the mycoestrogen-detoxifying lactonase ZHD. ZHD complex with beta-ZOL from Clonostachys rosea | biooc-ZHD101 |
| Gene_locus | Name | Species |
|---|---|---|
| 9euro-a0a0d2ilk1 |  Rhinocladiella mackenziei CBS 650.93 Z518_04590 Zhd518 Mycoestrogen-Detoxifying Lactonase |
Rhinocladiella mackenziei CBS 650.93 |
| xylba-a0a0d2h023 | Cladophialophora bantiana zearalenone hydrolase CbZHD |
Cladophialophora bantiana CBS 173.52 |
| biooc-ZHD101 | Bionectria ochroleuca (Gliocladium roseum), Clonostachys rosea f. rosea, zearalenone hydrolase ZHD101 ZEN-JJM |
Bionectria ochroleuca |
| 9pezi-a0a439d8r8 | Xylaria grammica. AB hydrolase-1 domain-containing protein | Xylaria grammica |
| 9pezi-a0a4z0yns1 | Xylaria hypoxylon. AB hydrolase-1 domain-containing protein | Xylaria hypoxylon |
| 9pezi-a0a553hs45 | Xylaria flabelliformis. AB hydrolase-1 domain-containing protein | Xylaria flabelliformis |
| 9euro-a0a072pad1 | Exophiala aquamarina CBS 119918. AB hydrolase-1 domain-containing protein |
Exophiala aquamarina CBS 119918 |
| eutla-m7tnt1 | Eutypa lata (strain UCR-EL1) (Grapevine dieback disease fungus) (Eutypa armeniacae). Putative alpha beta hydrolase fold protein | Eutypa lata |
| 9pezi-a0a4q4tp46 | Monosporascus sp. GIB2. AB hydrolase-1 domain-containing protein | Monosporascus sp. GIB2 |
| 9euro-a0a0d2e8j6 | Phialophora americana. ZHD607 lactone hydrolase | Phialophora americana |
| 9pezi-a0a4q4x369 | Monosporascus sp. CRB-9-2. AB hydrolase-1 domain-containing protein | Monosporascus sp. CRB-9-2 |
| 9euro-a0a0d2edi3 | Exophiala xenobiotica. AB hydrolase-1 domain-containing protein | Exophiala xenobiotica |
| 9pezi-a0a4v1x8l5 | Monosporascus ibericus. AB hydrolase-1 domain-containing protein | Monosporascus ibericus |
| 9pezi-a0a4q4ube4 | Monosporascus sp. MG133 thermostable zearalenone-degrading enzyme |
Monosporascus sp. MG133 |
| 9euro-w9x961 | Cladophialophora psammophila CBS 110553. AB hydrolase-1 domain-containing protein | Cladophialophora psammophila CBS 110553 |
| 9euro-a0a0d2b6n4 | Exophiala spinifera. AB hydrolase-1 domain-containing protein | Exophiala spinifera |
| 9euro-a0a178z8l1 | Fonsecaea erecta. AB hydrolase-1 domain-containing protein | Fonsecaea erecta |
| 9euro-w9vhd2 | Cladophialophora psammophila CBS 110553. AB hydrolase-1 domain-containing protein | Cladophialophora psammophila CBS 110553 |
| eutla-m7tmb1 | Eutypa lata (strain UCR-EL1) (Grapevine dieback disease fungus) (Eutypa armeniacae). Putative alpha beta hydrolase fold protein | Eutypa lata |
| exome-a0a0d2agi6 | Exophiala mesophila (Black yeast). AB hydrolase-1 domain-containing protein | Exophiala mesophila |
| rosne-a0a1w2tnw3 | Rosellinia necatrix (White root-rot fungus). Putative zearalenone lactonohydrolase | Rosellinia necatrix |
| 9euro-a0a0d2jr16 | Fonsecaea multimorphosa CBS 102226. AB hydrolase-1 domain-containing protein | Fonsecaea multimorphosa CBS 102226 |
| 9euro-a0a0d2h1w2 | Fonsecaea pedrosoi CBS 271.37. Unplaced genomic scaffold supercont1.5, whole genome shotgun sequence | Fonsecaea pedrosoi CBS 271.37 |
| 9pezi-a0a507bfx0 | Phialemoniopsis curvata. AB hydrolase-1 domain-containing protein | Phialemoniopsis curvata |
| 9pezi-a0a1y2ekn6 | Pseudomassariella vexata. Zearalenone hydrolase | Pseudomassariella vexata |
| 9euro-a0a0n0nqn9 | Phialophora attinorum. AB hydrolase-1 domain-containing protein | Phialophora attinorum |
| 9euro-a0a0n0nrd5 | Phialophora attinorum. AB hydrolase-1 domain-containing protein | Phialophora attinorum |
| 9helo-a0a2j6rsu8 | Hyaloscypha variabilis F. Alpha\/beta-hydrolase | Hyaloscypha variabilis F |
| 9euro-a0a0d1w8k5 | Exophiala sideris. alpha\/beta hydrolase (c-term PKS_ER domain)excluded | Exophiala sideris |
| 9helo-a0a2j6s3x0 | Hyaloscypha variabilis F. Alpha\/beta-hydrolase | Hyaloscypha variabilis F |
| 9helo-a0a1l7xua8 | Phialocephala subalpina. AB hydrolase-1 domain-containing protein | Phialocephala subalpina |
| 9helo-a0a370tt42 | Venustampulla echinocandica. AB hydrolase-1 domain-containing protein | Venustampulla echinocandica |
| 9helo-a0a2j6q8q8 | Pezoloma ericae. Alpha\/beta-hydrolase | Pezoloma ericae |
| 9helo-a0a194x7c3 | Phialocephala scopiformis. Alpha\/beta-hydrolase | Phialocephala scopiformis |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| Zearalenone | (2E,11S)-15,17-dihydroxy-11-methyl-12-oxabicyclo[12.4.0]octadeca-1(14),2,15,17-tetraene-7,13-dione | biooc-ZHD101 9baci-QBK11187 xylba-a0a0d2h023 9actn-ZenA 9noca-a0aa46n777 neucr-B15B24.080 9euro-a0a0d2ilk1 |
| Beta-zearalenol | (2E,7S,11S)-7,15,17-trihydroxy-11-methyl-12-oxabicyclo[12.4.0]octadeca-1(18),2,14,16-tetraen-13-one | biooc-ZHD101 |
| Alpha-zearalenol | (2E,7R,11S)-7,15,17-trihydroxy-11-methyl-12-oxabicyclo[12.4.0]octadeca-1(14),2,15,17-tetraen-13-one | biooc-ZHD101 |
| Inhibitor | Chemical Nomenclature | Proteins |
|---|---|---|
| ZGR | 2,4-dihydroxy-6-[(E,10S)-10-hydroxy-6-oxoundec-1-enyl]benzoic acid | biooc-ZHD101 9actn-ZenA 9noca-a0aa46n777 |
| ZFR | (E,10S)-1-(3,5-dihydroxyphenyl)-10-hydroxyundec-1-en-6-one | biooc-ZHD101 |
References (18)
| Title : Degradation mechanism for Zearalenone ring-cleavage by Zearalenone hydrolase RmZHD: A QM\/MM study - Zhou_2020_Sci.Total.Environ_709_135897 |
| Author(s) : Zhou J , Zhu L , Chen J , Wang W , Zhang R , Li Y , Zhang Q |
| Ref : Sci Total Environ , 709 :135897 , 2020 |
| Abstract : |
| PubMedSearch : Zhou_2020_Sci.Total.Environ_709_135897 |
| PubMedID: 31887512 |
| Gene_locus related to this paper: 9euro-a0a0d2ilk1 |
| Title : The Trp183 is essential in lactonohydrolase ZHD detoxifying zearalenone and zearalenols - Zhou_2020_Biochem.Biophys.Res.Commun_522_986 |
| Author(s) : Zhou H , Li L , Zhan B , Wang S , Li J , Hu XJ |
| Ref : Biochemical & Biophysical Research Communications , 522 :986 , 2020 |
| Abstract : |
| PubMedSearch : Zhou_2020_Biochem.Biophys.Res.Commun_522_986 |
| PubMedID: 31810602 |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Theoretical Study on Zearalenol Compounds Binding with Wild Type Zearalenone Hydrolase and V153H Mutant - Liu_2018_Int.J.Mol.Sci_19_ |
| Author(s) : Liu Y , Wan Y , Zhu J , Yu Z , Tian X , Han J , Zhang Z , Han W |
| Ref : Int J Mol Sci , 19 : , 2018 |
| Abstract : |
| PubMedSearch : Liu_2018_Int.J.Mol.Sci_19_ |
| PubMedID: 30231501 |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Esterase activity inspired selection and characterization of zearalenone degrading bacteria Bacillus pumilus ES-21 - Wang_2017_Food.Control_77_57 |
| Author(s) : Wang G , Yu M , Dong F , Shi J , Xu J |
| Ref : Food Control , 77 :57 , 2017 |
| Abstract : |
| PubMedSearch : Wang_2017_Food.Control_77_57 |
| PubMedID: |
| Title : Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana - Hui_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_515 |
| Author(s) : Hui R , Hu X , Liu W , Zheng Y , Chen Y , Guo RT , Jin J , Chen CC |
| Ref : Acta Crystallographica F Struct Biol Commun , 73 :515 , 2017 |
| Abstract : |
| PubMedSearch : Hui_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_515 |
| PubMedID: 28876230 |
| Gene_locus related to this paper: xylba-a0a0d2h023 |
| Title : The structure of a complex of the lactonohydrolase zearalenone hydrolase with the hydrolysis product of zearalenone at 1.60 A resolution - Qi_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_376 |
| Author(s) : Qi Q , Yang WJ , Zhou HJ , Ming DM , Sun KL , Xu TY , Hu XJ , Lv H |
| Ref : Acta Crystallographica F Struct Biol Commun , 73 :376 , 2017 |
| Abstract : |
| PubMedSearch : Qi_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_376 |
| PubMedID: 28695844 |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Isolation and characterisation of enzymatic zearalenone hydrolysis reaction products - Vekiru_2016_World.Mycotoxin.J_9_353 |
| Author(s) : Vekiru E , Fruhauf S , Hametner C , Schatzmayr G , Krska R , Moll WD , Schuhmacher R |
| Ref : World Mycotoxin Journal , 9 :353 , 2016 |
| Abstract : |
| PubMedSearch : Vekiru_2016_World.Mycotoxin.J_9_353 |
| PubMedID: |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : High-level expression of a ZEN-detoxifying gene by codon optimization and biobrick in Pichia pastoris - Xiang_2016_Microbiol.Res_193_48 |
| Author(s) : Xiang L , Wang Q , Zhou Y , Yin L , Zhang G , Ma Y |
| Ref : Microbiol Res , 193 :48 , 2016 |
| Abstract : |
| PubMedSearch : Xiang_2016_Microbiol.Res_193_48 |
| PubMedID: 27825486 |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea - Peng_2014_RSC.Adv_4_62321 |
| Author(s) : Peng W , Ko TP , Yang YY , Zheng YY , Chen CC , Zhu Z , Huang CH , Zeng YF , Huang JW , Wang AHJ , Liu JR , Guo RT |
| Ref : RSC Advances , 4 :62321 , 2014 |
| Abstract : |
| PubMedSearch : Peng_2014_RSC.Adv_4_62321 |
| PubMedID: |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Zearalenone lactonohydrolase activity in Hypocreales and its evolutionary relationships within the epoxide hydrolase subset of a\/b-hydrolases - Popiel_2014_BMC.Microbiol_14_82 |
| Author(s) : Popiel D , Koczyk G , Dawidziuk A , Gromadzka K , Blaszczyk L , Chelkowski J |
| Ref : BMC Microbiol , 14 :82 , 2014 |
| Abstract : |
| PubMedSearch : Popiel_2014_BMC.Microbiol_14_82 |
| PubMedID: 24708405 |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Zearalenone detoxification by zearalenone hydrolase is important for the antagonistic ability of Clonostachys rosea against mycotoxigenic Fusarium graminearum - Kosawang_2014_Fungal.Biol_118_364 |
| Author(s) : Kosawang C , Karlsson M , Velez H , Rasmussen PH , Collinge DB , Jensen B , Jensen DF |
| Ref : Fungal Biol , 118 :364 , 2014 |
| Abstract : |
| PubMedSearch : Kosawang_2014_Fungal.Biol_118_364 |
| PubMedID: 24742831 |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Reduced contamination by the Fusarium mycotoxin zearalenone in maize kernels through genetic modification with a detoxification gene - Igawa_2007_Appl.Environ.Microbiol_73_1622 |
| Author(s) : Igawa T , Takahashi-Ando N , Ochiai N , Ohsato S , Shimizu T , Kudo T , Yamaguchi I , Kimura M |
| Ref : Applied Environmental Microbiology , 73 :1622 , 2007 |
| Abstract : |
| PubMedSearch : Igawa_2007_Appl.Environ.Microbiol_73_1622 |
| PubMedID: 17209063 |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Two different polyketide synthase genes are required for synthesis of zearalenone in Gibberella zeae - Kim_2005_Mol.Microbiol_58_1102 |
| Author(s) : Kim YT , Lee YR , Jin J , Han KH , Kim H , Kim JC , Lee T , Yun SH , Lee YW |
| Ref : Molecular Microbiology , 58 :1102 , 2005 |
| Abstract : |
| PubMedSearch : Kim_2005_Mol.Microbiol_58_1102 |
| PubMedID: 16262793 |
| Gene_locus related to this paper: gibze-q2vlj3 |
| Title : A model transgenic cereal plant with detoxification activity for the estrogenic mycotoxin zearalenone - Higa-Nishiyama_2005_Transgenic.Res_14_713 |
| Author(s) : Higa-Nishiyama A , Takahashi-Ando N , Shimizu T , Kudo T , Yamaguchi I , Kimura M |
| Ref : Transgenic Res , 14 :713 , 2005 |
| Abstract : |
| PubMedSearch : Higa-Nishiyama_2005_Transgenic.Res_14_713 |
| PubMedID: 16245162 |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Efficient decontamination of zearalenone, the mycotoxin of cereal pathogen, by transgenic yeasts through the expression of a synthetic lactonohydrolase gene - Takahashi-Ando_2005_Appl.Microbiol.Biotechnol_67_838 |
| Author(s) : Takahashi-Ando N , Tokai T , Hamamoto H , Yamaguchi I , Kimura M |
| Ref : Applied Microbiology & Biotechnology , 67 :838 , 2005 |
| Abstract : |
| PubMedSearch : Takahashi-Ando_2005_Appl.Microbiol.Biotechnol_67_838 |
| PubMedID: 15630583 |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Metabolism of zearalenone by genetically modified organisms expressing the detoxification gene from Clonostachys rosea - Takahashi-Ando_2004_Appl.Environ.Microbiol_70_3239 |
| Author(s) : Takahashi-Ando N , Ohsato S , Shibata T , Hamamoto H , Yamaguchi I , Kimura M |
| Ref : Applied Environmental Microbiology , 70 :3239 , 2004 |
| Abstract : |
| PubMedSearch : Takahashi-Ando_2004_Appl.Environ.Microbiol_70_3239 |
| PubMedID: 15184117 |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Expression in cereal plants of genes that inactivate Fusarium mycotoxins - Higa_2003_Biosci.Biotechnol.Biochem_67_914 |
| Author(s) : Higa A , Kimura M , Mimori K , Ochiai-Fukuda T , Tokai T , Takahashi-Ando N , Nishiuchi T , Igawa T , Fujimura M , Hamamoto H , Usami R , Yamaguchi I |
| Ref : Biosci Biotechnol Biochem , 67 :914 , 2003 |
| Abstract : |
| PubMedSearch : Higa_2003_Biosci.Biotechnol.Biochem_67_914 |
| PubMedID: 12784641 |
| Gene_locus related to this paper: biooc-ZHD101 |
| Title : Purification and characterization of a lactonase from Erwinia cypripedii 314B that hydrolyzes ( S)-5-oxo-2-tetrahydrofurancarboxylic acid - Mochizuki_2003_Arch.Microbiol_180_490 |
| Author(s) : Mochizuki K |
| Ref : Arch Microbiol , 180 :490 , 2003 |
| Abstract : |
| PubMedSearch : Mochizuki_2003_Arch.Microbiol_180_490 |
| PubMedID: 14576976 |
| Gene_locus related to this paper: biooc-ZHD101 |