Velan_1996_FEBS.Lett_395_22

Reference

Title : Structural modifications of the omega loop in human acetylcholinesterase - Velan_1996_FEBS.Lett_395_22
Author(s) : Velan B , Barak D , Ariel N , Leitner M , Bino T , Ordentlich A , Shafferman A
Ref : FEBS Letters , 395 :22 , 1996
Abstract : Conformational mobility of the surface omega loop (Cys-69-Cys-96) in human acetylcholinesterase (HuAChE) was recently implicated in substrate accessibility to the active center and in the mechanism of allosteric modulation of enzymatic activity. We therefore generated and kinetically evaluated the following modifications or replacements in HuAChE: (a) residues at the loop ends, (b) residues involved in putative hydrogen-bond interactions within the loop and between the loop and the protein core, (c) ChEs conserved proline residues within the loop and (d) a deletion of a conserved segment of 5 residues. All the residue replacements, including those of the prolines, had either limited or no effect on enzyme reactivity. These results suggest that unlike the case of lipase, the omega loop in the HuAChE is not involved in large lid-like displacements. In cases where modifications of the loop sequence had some effect on reactivity, the effects could be attributed to an altered position of residue Trp-86 supporting the proposed coupling between the structure of the omega loop and the positioning of the Trp-86 indole moiety, in catalytic activity and in allosterism.
ESTHER : Velan_1996_FEBS.Lett_395_22
PubMedSearch : Velan_1996_FEBS.Lett_395_22
PubMedID: 8849682

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Citations formats

Velan B, Barak D, Ariel N, Leitner M, Bino T, Ordentlich A, Shafferman A (1996)
Structural modifications of the omega loop in human acetylcholinesterase
FEBS Letters 395 :22

Velan B, Barak D, Ariel N, Leitner M, Bino T, Ordentlich A, Shafferman A (1996)
FEBS Letters 395 :22