Title : The abundance of mRNA transcripts of bacteroidetal polyethylene terephthalate (PET) esterase genes may indicate a role in marine plastic degradation - Zhang_2021_ResearchSquare__ |
Author(s) : Zhang H , Dierkes R , Perez-Garcia P , Weigert S , Sternagel S , Hallam S , Schott T , Juergens K , Vollstedt C , Chibani C , Danso D , Buchholz PCF , Pleiss J , Almeida A , Hocker B , Schmitz R , Chow J , Streit WR |
Ref : ResearchSquare , : , 2021 |
Abstract :
https://www.researchsquare.com/article/rs-567691/v2 Polyethylene terephthalate (PET) is an important synthetic polymer accumulating in nature 2 and recent studies have identified microorganisms capable of degrading PET. While the majority of 3 known PET hydrolases originate from the Actinobacteria and Proteobacteria, here we describe the 4 first functional PET-active enzymes from the Bacteroidetes phylum. Using a PETase-specific 5 Hidden-Markov-Model (HMM)-based search algorithm we identified two promiscuous and cold6 active esterases derived from Aequorivita sp. (PET27) and Chryseobacterium jeonii (PET30) acting 7 on PET foil and powder. Notably, one of the enzymes (PET30) was able to hydrolyze PET at 8 temperatures between 4 - 30 C with a similar turnover rate compared to the well-known Ideonella 9 sakaiensis enzyme (IsPETase). 10 PET27 and PET30 homologues were detected in metagenomes encompassing a wide range 11 of different global climate zones. Additional transcript abundance mapping of marine samples imply 12 that these promiscuous enzymes and source organisms may play a role in the long-term 13 degradation of microplastic particles and fibers. |
PubMedSearch : Zhang_2021_ResearchSquare__ |
PubMedID: |
Gene_locus related to this paper: flutr-f2ie04 , 9flao-a0a0c1f4u8 , 9flao-kjj39608 , 9flao-a0a1m6f5v0 , 9flao-a0a330mq60 |
Zhang H, Dierkes R, Perez-Garcia P, Weigert S, Sternagel S, Hallam S, Schott T, Juergens K, Vollstedt C, Chibani C, Danso D, Buchholz PCF, Pleiss J, Almeida A, Hocker B, Schmitz R, Chow J, Streit WR (2021)
The abundance of mRNA transcripts of bacteroidetal polyethylene terephthalate (PET) esterase genes may indicate a role in marine plastic degradation
ResearchSquare
:
Zhang H, Dierkes R, Perez-Garcia P, Weigert S, Sternagel S, Hallam S, Schott T, Juergens K, Vollstedt C, Chibani C, Danso D, Buchholz PCF, Pleiss J, Almeida A, Hocker B, Schmitz R, Chow J, Streit WR (2021)
ResearchSquare
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