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Substrate Report for: Fluorescein-di(diethylphosphate)

A fluorogenic substrate for detection of organophosphatase activity


General
Type Fluorescent Probe, Benzofuran, Organophosphate, Flu-OP
Chemical_Nomenclature
Canonical SMILES CCOP(=O)(OCC)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OP(=O)(OCC)OCC)C5=CC=CC=C5C(=O)O3
InChI InChI=1S/C28H30O11P2/c1-5-32-40(30,33-6-2)38-19-13-15-23-25(17-19)36-26-18-20(39-41(31,34-7-3)35-8-4)14-16-24(26)28(23)22-12-10-9-11-21(22)27(29)37-28/h9-18H,5-8H2,1-4H3
InChIKey XTQFRHYOPAKUAK-UHFFFAOYSA-N
Other name(s) FDDEP ; Tetraethyl (3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl) bis(phosphate) ; (6'-diethoxyphosphoryloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) diethyl phosphate
________________________________________________________________________________________________
MW|604.5
Formula|C28H30O11P2
CAS_number|
PubChem|11467555
UniChem|XTQFRHYOPAKUAK-UHFFFAOYSA-N
IUPHAR|
Wikipedia|

Target
Families | Fluorescein-di(diethylphosphate) ligand of proteins in family: Dienelactone_hydrolase
Protein | 9gamm-a0a346myw6, 9gamm-m3DLH

References:
Search PubMed for references concerning: Fluorescein-di(diethylphosphate)
    Title: Ultrahigh-throughput directed evolution of a metal-free alpha/beta-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase
    Schnettler-Fernandez D, Klein OJ, Kaminski TS, Colin PY, Hollfelder F
    Ref: Biorxiv, :, 2022 : PubMed

            

    Title: Evolution of enzyme catalysts caged in biomimetic gel-shell beads
    Fischlechner M, Schaerli Y, Mohamed MF, Patil S, Abell C, Hollfelder F
    Ref: Nat Chem, 6:791, 2014 : PubMed

            

    Title: A fluorogenic substrate for detection of organophosphatase activity
    Soukharev S, Hammond DJ
    Ref: Analytical Biochemistry, 327:140, 2004 : PubMed