Patil S

References (10)

Title : Barbaloin's Chemical Intervention in Aluminum Chloride Induced Cognitive Deficits and Changes in Rats through Modulation of Oxidative Stress, Cytokines, and BDNF Expression - Kazmi_2024_ACS.Omega_9_6976
Author(s) : Kazmi I , Afzal M , Imam F , Alzarea SI , Patil S , Mhaiskar A , Shah U , Almalki WH
Ref : ACS Omega , 9 :6976 , 2024
Abstract : Alzheimer's disease (AD) is a long-term neurodegenerative condition characterized by impaired cognitive functions, particularly in the domains of learning and memory. Finding promising options for AD can be successful with a medication repurposing strategy. The goal of the research was to examine the neuroprotective characteristics of barbaloin in aluminum chloride (AlCl(3))-induced cognitive deficits and changes in rats through modulation of oxidative stress, cytokines, and brain-derived neurotrophic factor (BDNF) expression. Thirty male Wistar rats were subjected to AlCl(3) at a dosage of 100 mg/kg via the per oral route (p.o.), which induced cognitive decline. Morris water maze (MWM) is used to assess behavioral metrics. Assays for catalase (CAT), malondialdehyde (MDA), reduced glutathione (GSH), acetylcholinesterase (AChE), choline-acetyltransferase (ChAT), interleukins-1beta (IL-1beta), superoxide dismutase (SOD), tumor necrosis factor-alpha (TNF-alpha), nuclear factor kappa-B (NF-kappaB), interleukins-6 (IL-6), BDNF, and neurotransmitter levels [dopamine (DA), acetylcholine (Ach), and gamma-aminobutyric acid (GABA)] were performed. Results: The transfer latency time was notably decreased, and substantial modifications in the concentrations of GSH, MDA, CAT, SOD, AChE, ChAT and observed modulations in the formation of interleukins-6 (IL-6), TNF-alpha, IL-1beta, BDNF, and NF-kappaB were also evidenced after the treatment of rats with barbaloin in comparison to AlCl(3)-induced control groups. Significant alterations in neurotransmitter levels (DA, Ach, and GABA) were also seen in barbaloin-treated groups in comparison to AlCl(3)-induced groups. The current investigation has provided evidence that the administration of barbaloin yielded notable enhancements in cognitive function in rats through the inhibition of MDA, enhancing endogenous antioxidant enzymes, reduction of cytokine levels, and enhancement of neurotransmitter contents in the brain. These effects were observed in comparison to a control group treated with AlCl(3) and can be attributable to barbaloin's strong anti-inflammatory and antioxidant properties, and metal chelating properties may contribute to its neuroprotective effects. Barbaloin may also promote neuronal survival and enhance learning and memory by upregulating the expression of BDNF.
ESTHER : Kazmi_2024_ACS.Omega_9_6976
PubMedSearch : Kazmi_2024_ACS.Omega_9_6976
PubMedID: 38371830

Title : Neuroprotective Effect of Barbaloin on Streptozotocin-Induced Cognitive Dysfunction in Rats via Inhibiting Cholinergic and Neuroinflammatory Cytokines Pathway-TNF-alpha\/IL-1beta\/IL-6\/NF-B - Omer_2023_ACS.Omega_8_8110
Author(s) : Omer AB , Afzal O , Altamimi ASA , Patil S , AlGhamdi SA , Alghamdi AM , Alzarea SI , Almalki WH , Kazmi I
Ref : ACS Omega , 8 :8110 , 2023
Abstract : Streptozotocin (STZ) impairs memory in rats through altering the central nervous systems (CNS) as a result of impaired cholinergic dysfunction, oxidative stress, persistent hyperglycemia, and alterations in the glucagon-like peptide (GLP). In this model cholinergic agonist, antioxidant and antihyperglycemic treatment has been shown to have positive effects. Barbaloin has a variety of pharmacological effects. However, there is no evidence on how barbaloin improves memory dysfunction caused by STZ. Thus, we examined its effectiveness against cognitive damage caused by STZ at a dose of 60 mg/kg i.p. in Wistar rats. Blood glucose levels (BGL) and body weight (BW) were assessed. To assess learning and memory skills, the Y-maze test and Morris water maze (MWM) test were utilized. Superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and glutathione (GSH) as oxidative stress markers were regulated to reverse the cognitive deterioration, and choline-acetyltransferase (ChAT) and acetyl-cholinesterase (AChE) as indicators of cholinergic dysfunction, nuclear factor kappa-B (NF-kappaB), IL-1beta (interleukin-1beta), IL-6, and tumor necrosis factor-alpha (TNF-alpha) contents were used. Barbaloin treatment thereby significantly decreased the BW and learning and memory capacities, resulting in substantial behavioral improvement in the Y-maze and MWM test. BGL, SOD, CAT, MDA, GSH, AChE, ChAT, NF-kappaB, IL-6, TNF-alpha, and IL-1beta levels were also altered. In conclusion, the findings revealed that barbaloin had a protective impact against cognitive dysfunction caused by STZ.
ESTHER : Omer_2023_ACS.Omega_8_8110
PubMedSearch : Omer_2023_ACS.Omega_8_8110
PubMedID: 36872976

Title : Multidimensional Studies of Pancratium parvum Dalzell Against Acetylcholinesterase: A Potential Enzyme for Alzheimer's Management - Patil_2020_J.Am.Coll.Nutr__1
Author(s) : Patil DN , Yadav SR , Patil S , Bapat VA , Jadhav JP
Ref : J Am Coll Nutr , :1 , 2020
Abstract : Objective: Pancratium L. (Amaryllidaceae J.St. Hil.) is a monocot genus with bulbous habitat and about 20 species worldwide have significant medicinal properties. The present envision aims to investigate the potential ability of Pancratium species for acetylcholinesterase (AChE) inhibition as a remedy for Alzheimer disease (AD). Different Pancratium species were screened for the inhibition of AChE enzyme from various localities across India. Prominent species was further studied for anti-inflammatory, antioxidant, metal chelating and UHPLC-QTOF-MS analysis.Methods: Nine different species collected across India were examined for AChE inhibition and for binding affinity studies using Surface Plasmon Resonance (SPR). Highest inhibition species was subjected to Response Surface Methodology (RSM) to accomplish the effective conditions for maximum extraction of phytomolecules in accordance with the inhibition of the AChE. Further, extract under optimized conditions were used to study anti-inflammatory, antioxidant, metal chelating and UHPLC-QTOF-MS analysis for tentative identification of phytomolecules.Results: Amongst different species collected, P. parvum Dalzell exhibited maximum inhibition 93.30 +/- 1.71% with promising IC50 20 +/- 0.22 microg/ml value. In addition, binding affinity toward AChE and beta plaques using SPR technique showed a higher binding response toward the enzyme. RSM study resulted that water extracts at 50 degrees C and 5.46 hours heating executed maximum inhibition. Other studies showed prominent anti-inflammatory and metal chelating ability with low antioxidant property.Conclusion: By using UHPLC-QTOF-MS compounds were tentatively identified for the concerned activities mentioned above. This work reports for accounting the detailed study of P. parvum and which can be further entailed for the treatment of various neurological disorders.
ESTHER : Patil_2020_J.Am.Coll.Nutr__1
PubMedSearch : Patil_2020_J.Am.Coll.Nutr__1
PubMedID: 31951787

Title : Protective effect of berberine, an isoquinoline alkaloid ameliorates ethanol-induced oxidative stress and memory dysfunction in rats - Patil_2015_Pharmacol.Biochem.Behav_136_13
Author(s) : Patil S , Tawari S , Mundhada D , Nadeem S
Ref : Pharmacol Biochem Behav , 136 :13 , 2015
Abstract : Memory impairment induced by ethanol in rats is a consequence of changes in the CNS that are secondary to impaired oxidative stress and cholinergic dysfunction. Treatment with antioxidants and cholinergic agonists are reported to produce beneficial effects in this model. Berberine, an isoquinoline alkaloid is reported to exhibit antioxidant effect and cholinesterase (ChE) inhibitor activity. However, no report is available on the influence of berberine on ethanol-induced memory impairment. Therefore, we tested its influence against cognitive dysfunction in ethanol-induced rats using Morris water maze paradigm. Lipid peroxidation and glutathione levels as parameter of oxidative stress and cholinesterase (ChE) activity as a marker of cholinergic function were assessed in the cerebral cortex and hippocampus. Forty five days after ethanol treated rats showed a severe deficit in learning and memory associated with increased lipid peroxidation, decreased glutathione, and elevated ChE activity. In contrast, chronic treatment with berberine (25-100mg/kg, p.o., once a day for 45days) improved cognitive performance, and lowered oxidative stress and ChE activity in ethanol treated rats. In another set of experiments, berberine (100mg/kg) treatment during training trials also improved learning and memory, and lowered oxidative stress and ChE activity. Chronic treatment (45days) with vitamin C, and donepezil during training trials also improved ethanol-induced memory impairment and reduced oxidative stress and/or cholinesterase activity. In conclusion, the present study demonstrates that treatment with berberine prevents the changes in oxidative stress and ChE activity, and consequently memory impairment in ethanol treated rats.
ESTHER : Patil_2015_Pharmacol.Biochem.Behav_136_13
PubMedSearch : Patil_2015_Pharmacol.Biochem.Behav_136_13
PubMedID: 26159088

Title : Comparative Study to Predict Dipeptidyl Peptidase IV Inhibitory Activity of beta-Amino Amide Scaffold - Patil_2015_Indian.J.Pharm.Sci_77_142
Author(s) : Patil S , Sharma R , Abhishek A
Ref : Indian J Pharm Sci , 77 :142 , 2015
Abstract : Comparative study was performed on 34 beta-amino amide derivatives as dipeptidyl peptidase IV inhibitors in order to determine their structural requirement to enhance the antidiabetic activities. Hologram quantitative structure activity relationships models utilized specialized fragment fingerprints (hologram length 353) which showed good predictivity with cross-validated q(2) and conventional r(2) values of 0.971 and 0.971, respectively. Models were validated and optimized by a test set of eight compounds and gave satisfactory predictive ability. Hologram quantitative structure activity relationships maps were helpful in prediction of the structural features of the ligands to account for the activity in terms of positively and negatively contributing towards activity. The information obtained from maps could be effectively use as a guiding tool for further structure modifications and synthesis of new potent antidiabetic agents.
ESTHER : Patil_2015_Indian.J.Pharm.Sci_77_142
PubMedSearch : Patil_2015_Indian.J.Pharm.Sci_77_142
PubMedID: 26009645

Title : Evolution of enzyme catalysts caged in biomimetic gel-shell beads - Fischlechner_2014_Nat.Chem_6_791
Author(s) : Fischlechner M , Schaerli Y , Mohamed MF , Patil S , Abell C , Hollfelder F
Ref : Nat Chem , 6 :791 , 2014
Abstract : Natural evolution relies on the improvement of biological entities by rounds of diversification and selection. In the laboratory, directed evolution has emerged as a powerful tool for the development of new and improved biomolecules, but it is limited by the enormous workload and cost of screening sufficiently large combinatorial libraries. Here we describe the production of gel-shell beads (GSBs) with the help of a microfluidic device. These hydrogel beads are surrounded with a polyelectrolyte shell that encloses an enzyme, its encoding DNA and the fluorescent reaction product. Active clones in these man-made compartments can be identified readily by fluorescence-activated sorting at rates >10(7) GSBs per hour. We use this system to perform the directed evolution of a phosphotriesterase (a bioremediation catalyst) caged in GSBs and isolate a 20-fold faster mutant in less than one hour. We thus establish a practically undemanding method for ultrahigh-throughput screening that results in functional hybrid composites endowed with evolvable protein components.
ESTHER : Fischlechner_2014_Nat.Chem_6_791
PubMedSearch : Fischlechner_2014_Nat.Chem_6_791
PubMedID: 25143214

Title : Protection of cholinergic and antioxidant system contributes to the effect of berberine ameliorating memory dysfunction in rat model of streptozotocin-induced diabetes - Bhutada_2011_Behav.Brain.Res_220_30
Author(s) : Bhutada P , Mundhada Y , Bansod K , Tawari S , Patil S , Dixit P , Umathe S , Mundhada D
Ref : Behavioural Brain Research , 220 :30 , 2011
Abstract : Memory impairment induced by streptozotocin in rats is a consequence of changes in CNS that are secondary to chronic hyperglycemia, impaired oxidative stress, cholinergic dysfunction, and changes in glucagon-like peptide (GLP). Treatment with antihyperglycemics, antioxidants, and cholinergic agonists are reported to produce beneficial effect in this model. Berberine, an isoquinoline alkaloid is reported to exhibit anti-diabetic and antioxidant effect, acetylcholinesterase (AChE) inhibitor, and increases GLP release. However, no report is available on influence of berberine on streptozotocin-induced memory impairment. Therefore, we tested its influence against cognitive dysfunction in streptozotocin-induced diabetic rats using Morris water maze paradigm. Lipid peroxidation and glutathione levels as parameters of oxidative stress and choline esterase (ChE) activity as marker of cholinergic function were assessed in the cerebral cortex and hippocampus. Thirty days after diabetes induction rats showed a severe deficit in learning and memory associated with increased lipid peroxidation, decreased reduced glutathione, and elevated ChE activity. In contrast, chronic treatment with berberine (25-100mg/kg, p.o., twice daily, 30 days) improved cognitive performance, lowered hyperglycemia, oxidative stress, and ChE activity in diabetic rats. In another set of experiment, berberine (100mg/kg) treatment during training trials also improved learning and memory, lowered hyperglycemia, oxidative stress, and ChE activity. Chronic treatment (30 days) with vitamin C or metformin, and donepezil during training trials also improved diabetes-induced memory impairment and reduced oxidative stress and/or choline esterase activity. In conclusion, the present study demonstrates treatment with berberine prevents the changes in oxidative stress and ChE activity, and consequently memory impairment in diabetic rats.
ESTHER : Bhutada_2011_Behav.Brain.Res_220_30
PubMedSearch : Bhutada_2011_Behav.Brain.Res_220_30
PubMedID: 21262264

Title : Lipase of Aspergillus niger NCIM 1207: A Potential Biocatalyst for Synthesis of Isoamyl Acetate - Mhetras_2010_Indian.J.Microbiol_50_432
Author(s) : Mhetras N , Patil S , Gokhale D
Ref : Indian J Microbiol , 50 :432 , 2010
Abstract : Commercial lipase preparations and mycelium bound lipase from Aspergillus niger NCIM 1207 were used for esterification of acetic acid with isoamyl alcohol to obtain isoamyl acetate. The esterification reaction was carried out at 30 degreesC in n-hexane with shaking at 120 rpm. Initial reaction rates, conversion efficiency and isoamyl acetate concentration obtained using Novozyme 435 were the highest. Mycelium bound lipase of A. niger NCIM 1207 produced maximal isoamyl acetate formation at an alcohol/acid ratio of 1.6. Acetic acid at higher concentrations than required for the critical alcohol/acid ratio lower than 1.3 and higher than 1.6 resulted in decreased yields of isoamyl acetate probably owing to lowering of micro-aqueous environmental pH around the enzyme leading to inhibition of enzyme activity. Mycelium bound A. niger lipase produced 80 g/l of isoamyl acetate within 96 h even though extremely less amount of enzyme activity was used for esterification. The presence of sodium sulphate during esterification reaction at higher substrate concentration resulted in increased conversion efficiency when we used mycelium bound enzyme preparations of A. niger NCIM 1207. This could be due to removal of excess water released during esterification reaction by sodium sulphate. High ester concentration (286.5 g/l) and conversion (73.5%) were obtained within 24 h using Novozyme 435 under these conditions.
ESTHER : Mhetras_2010_Indian.J.Microbiol_50_432
PubMedSearch : Mhetras_2010_Indian.J.Microbiol_50_432
PubMedID: 22282611

Title : Evolutionary and biomedical insights from the rhesus macaque genome - Gibbs_2007_Science_316_222
Author(s) : Gibbs RA , Rogers J , Katze MG , Bumgarner R , Weinstock GM , Mardis ER , Remington KA , Strausberg RL , Venter JC , Wilson RK , Batzer MA , Bustamante CD , Eichler EE , Hahn MW , Hardison RC , Makova KD , Miller W , Milosavljevic A , Palermo RE , Siepel A , Sikela JM , Attaway T , Bell S , Bernard KE , Buhay CJ , Chandrabose MN , Dao M , Davis C , Delehaunty KD , Ding Y , Dinh HH , Dugan-Rocha S , Fulton LA , Gabisi RA , Garner TT , Godfrey J , Hawes AC , Hernandez J , Hines S , Holder M , Hume J , Jhangiani SN , Joshi V , Khan ZM , Kirkness EF , Cree A , Fowler RG , Lee S , Lewis LR , Li Z , Liu YS , Moore SM , Muzny D , Nazareth LV , Ngo DN , Okwuonu GO , Pai G , Parker D , Paul HA , Pfannkoch C , Pohl CS , Rogers YH , Ruiz SJ , Sabo A , Santibanez J , Schneider BW , Smith SM , Sodergren E , Svatek AF , Utterback TR , Vattathil S , Warren W , White CS , Chinwalla AT , Feng Y , Halpern AL , Hillier LW , Huang X , Minx P , Nelson JO , Pepin KH , Qin X , Sutton GG , Venter E , Walenz BP , Wallis JW , Worley KC , Yang SP , Jones SM , Marra MA , Rocchi M , Schein JE , Baertsch R , Clarke L , Csuros M , Glasscock J , Harris RA , Havlak P , Jackson AR , Jiang H , Liu Y , Messina DN , Shen Y , Song HX , Wylie T , Zhang L , Birney E , Han K , Konkel MK , Lee J , Smit AF , Ullmer B , Wang H , Xing J , Burhans R , Cheng Z , Karro JE , Ma J , Raney B , She X , Cox MJ , Demuth JP , Dumas LJ , Han SG , Hopkins J , Karimpour-Fard A , Kim YH , Pollack JR , Vinar T , Addo-Quaye C , Degenhardt J , Denby A , Hubisz MJ , Indap A , Kosiol C , Lahn BT , Lawson HA , Marklein A , Nielsen R , Vallender EJ , Clark AG , Ferguson B , Hernandez RD , Hirani K , Kehrer-Sawatzki H , Kolb J , Patil S , Pu LL , Ren Y , Smith DG , Wheeler DA , Schenck I , Ball EV , Chen R , Cooper DN , Giardine B , Hsu F , Kent WJ , Lesk A , Nelson DL , O'Brien W E , Prufer K , Stenson PD , Wallace JC , Ke H , Liu XM , Wang P , Xiang AP , Yang F , Barber GP , Haussler D , Karolchik D , Kern AD , Kuhn RM , Smith KE , Zwieg AS
Ref : Science , 316 :222 , 2007
Abstract : The rhesus macaque (Macaca mulatta) is an abundant primate species that diverged from the ancestors of Homo sapiens about 25 million years ago. Because they are genetically and physiologically similar to humans, rhesus monkeys are the most widely used nonhuman primate in basic and applied biomedical research. We determined the genome sequence of an Indian-origin Macaca mulatta female and compared the data with chimpanzees and humans to reveal the structure of ancestral primate genomes and to identify evidence for positive selection and lineage-specific expansions and contractions of gene families. A comparison of sequences from individual animals was used to investigate their underlying genetic diversity. The complete description of the macaque genome blueprint enhances the utility of this animal model for biomedical research and improves our understanding of the basic biology of the species.
ESTHER : Gibbs_2007_Science_316_222
PubMedSearch : Gibbs_2007_Science_316_222
PubMedID: 17431167
Gene_locus related to this paper: macmu-3neur , macmu-ACHE , macmu-BCHE , macmu-f6rul6 , macmu-f6sz31 , macmu-f6the6 , macmu-f6unj2 , macmu-f6wtx1 , macmu-f6zkq5 , macmu-f7aa58 , macmu-f7ai42 , macmu-f7aim4 , macmu-f7buk8 , macmu-f7cfi8 , macmu-f7cnr2 , macmu-f7cu68 , macmu-f7flv1 , macmu-f7ggk1 , macmu-f7hir7 , macmu-g7n054 , macmu-KANSL3 , macmu-TEX30 , macmu-Y4neur , macmu-g7n4x3 , macmu-i2cy02 , macmu-f7ba84 , macmu-CES2 , macmu-h9er02 , macmu-a0a1d5rbr3 , macmu-a0a1d5q4k5 , macmu-g7mxj6 , macmu-f7dn71 , macmu-f7hkw9 , macmu-f7hm08 , macmu-g7mke4 , macmu-a0a1d5rh04 , macmu-h9fud6 , macmu-f6qwx1 , macmu-f7h4t2 , macmu-h9zaw9 , macmu-f7h550 , macmu-a0a1d5q9w1 , macmu-f7gkb9 , macmu-f7hp78 , macmu-a0a1d5qvu5

Title : Tannin acyl hydrolase (EC activity of Aspergillus, Penicillium, Fusarium and Trichoderma - Bajpai_1996_World.J.Microbiol.Biotechnol_12_217
Author(s) : Bajpai B , Patil S
Ref : World J Microbiol Biotechnol , 12 :217 , 1996
Abstract : A spectrophotometric method to determine gallic acid, residual gallotannin and tannin acyl hydrolase (EC activity during microbial hydrolysis of pentagalloyl glucose is described. The following equations have been developed to estimate gallotannin and gallic acid in the incubation medium by absorbance measurements at two different wavelengths: concentration of gallotannin (microg ml(-1))=34.41 (A293.8)-6.98 (A254.6); concentration of gallic acid (microg ml(-1))=21.77 (A254.6)-17.17 (A293.8). As compared to Aspergillus and Penicillium, the fungal genera extensively studied for the production of this enzyme, Fusarium solanii and Trichoderma viride exhibited higher enzyme activity showing approximately 88 and 84 mole percent conversion respectively after a 24 h incubation period.
ESTHER : Bajpai_1996_World.J.Microbiol.Biotechnol_12_217
PubMedSearch : Bajpai_1996_World.J.Microbiol.Biotechnol_12_217
PubMedID: 24415228