Abubucker S

References (3)

Title : Genome of the human hookworm Necator americanus - Tang_2014_Nat.Genet_46_261
Author(s) : Tang YT , Gao X , Rosa BA , Abubucker S , Hallsworth-Pepin K , Martin J , Tyagi R , Heizer E , Zhang X , Bhonagiri-Palsikar V , Minx P , Warren WC , Wang Q , Zhan B , Hotez PJ , Sternberg PW , Dougall A , Gaze ST , Mulvenna J , Sotillo J , Ranganathan S , Rabelo EM , Wilson RK , Felgner PL , Bethony J , Hawdon JM , Gasser RB , Loukas A , Mitreva M
Ref : Nat Genet , 46 :261 , 2014
Abstract : The hookworm Necator americanus is the predominant soil-transmitted human parasite. Adult worms feed on blood in the small intestine, causing iron-deficiency anemia, malnutrition, growth and development stunting in children, and severe morbidity and mortality during pregnancy in women. We report sequencing and assembly of the N. americanus genome (244 Mb, 19,151 genes). Characterization of this first hookworm genome sequence identified genes orchestrating the hookworm's invasion of the human host, genes involved in blood feeding and development, and genes encoding proteins that represent new potential drug targets against hookworms. N. americanus has undergone a considerable and unique expansion of immunomodulator proteins, some of which we highlight as potential treatments against inflammatory diseases. We also used a protein microarray to demonstrate a postgenomic application of the hookworm genome sequence. This genome provides an invaluable resource to boost ongoing efforts toward fundamental and applied postgenomic research, including the development of new methods to control hookworm and human immunological diseases.
ESTHER : Tang_2014_Nat.Genet_46_261
PubMedSearch : Tang_2014_Nat.Genet_46_261
PubMedID: 24441737
Gene_locus related to this paper: necam-w2tsu7

Title : The draft genome of the parasitic nematode Trichinella spiralis - Mitreva_2011_Nat.Genet_43_228
Author(s) : Mitreva M , Jasmer DP , Zarlenga DS , Wang Z , Abubucker S , Martin J , Taylor CM , Yin Y , Fulton L , Minx P , Yang SP , Warren WC , Fulton RS , Bhonagiri V , Zhang X , Hallsworth-Pepin K , Clifton SW , McCarter JP , Appleton J , Mardis ER , Wilson RK
Ref : Nat Genet , 43 :228 , 2011
Abstract : Genome evolution studies for the phylum Nematoda have been limited by focusing on comparisons involving Caenorhabditis elegans. We report a draft genome sequence of Trichinella spiralis, a food-borne zoonotic parasite, which is the most common cause of human trichinellosis. This parasitic nematode is an extant member of a clade that diverged early in the evolution of the phylum, enabling identification of archetypical genes and molecular signatures exclusive to nematodes. We sequenced the 64-Mb nuclear genome, which is estimated to contain 15,808 protein-coding genes, at approximately 35-fold coverage using whole-genome shotgun and hierarchal map-assisted sequencing. Comparative genome analyses support intrachromosomal rearrangements across the phylum, disproportionate numbers of protein family deaths over births in parasitic compared to a non-parasitic nematode and a preponderance of gene-loss and -gain events in nematodes relative to Drosophila melanogaster. This genome sequence and the identified pan-phylum characteristics will contribute to genome evolution studies of Nematoda as well as strategies to combat global parasites of humans, food animals and crops.
ESTHER : Mitreva_2011_Nat.Genet_43_228
PubMedSearch : Mitreva_2011_Nat.Genet_43_228
PubMedID: 21336279
Gene_locus related to this paper: trisp-ACHE1 , trisp-e5ryh1 , trisp-e5s2p1 , trisp-e5s3s1 , trisp-e5s5l6 , trisp-e5s7y8 , trisp-e5s8m6 , trisp-e5s9j3 , trisp-e5s254 , trisp-e5s773 , trisp-e5sav1 , trisp-e5sbp4 , trisp-e5sgg4 , trisp-e5sgu8 , trisp-e5snw0 , trisp-e5sr61 , trisp-e5ss42 , trisp-e5sgh2 , 9bila-a0a0v0tgw4.1 , 9bila-a0a0v0tws5

Title : Transcripts analysis of the entomopathogenic nematode Steinernema carpocapsae induced in vitro with insect haemolymph - Hao_2010_Mol.Biochem.Parasitol_169_79
Author(s) : Hao YJ , Montiel R , Abubucker S , Mitreva M , Simoes N
Ref : Molecular & Biochemical Parasitology , 169 :79 , 2010
Abstract : Steinernema carpocapsae is an insect parasitic nematode widely used in pest control programs. The efficacy of this nematode in controlling insects has been found to be related to the pathogenicity of the infective stage. In order to study the parasitic mechanisms exhibited by this parasite, a cDNA library of the induced S. carpocapsae parasitic phase was generated. A total of 2500 clones were sequenced and 2180 high-quality ESTs were obtained from this library. Cluster analysis generated a total of 1592 unique sequences including 1393 singletons. About 63% of the unique sequences had significant hits (e<=1e-05) to the non-redundant protein database. The remaining sequences most likely represent putative novel protein coding genes. Comparative analysis identified 377 homologs in C. elegans, 431 in C. briggsae and 75 in other nematodes. Classification of the predicted proteins revealed involvement in diverse cellular, metabolic and extracellular functions. One hundred and nineteen clusters were predicted to encode putative secreted proteins such as proteases, proteases inhibitors, lectins, saposin-like proteins, acetylcholinesterase, anti-oxidants, and heat-shock proteins, which can possibly have host interactions. This dataset provides a basis for genomic studies towards a better understanding of the events that occur in the parasitic process of this entomopathogenic nematode, including invasion of the insect haemocoelium, adaptations to insect innate immunity and stress responses, and production of virulence factors. The identification of key genes in the parasitic process provides useful tools for the improvement of S. carpocapsae as a biological agent.
ESTHER : Hao_2010_Mol.Biochem.Parasitol_169_79
PubMedSearch : Hao_2010_Mol.Biochem.Parasitol_169_79
PubMedID: 19836423