Kasai S

References (11)

Title : Frequencies and distribution of kdr and Ace alleles that cause insecticide resistance in house flies in the United States - Mertz_2023_Pestic.Biochem.Physiol_194_105497
Author(s) : Mertz RW , Dressel AE , Fisher CR , Moon RD , Donahue WA , Kasai S , Scott JG
Ref : Pestic Biochem Physiol , 194 :105497 , 2023
Abstract : House flies (Musca domestica L) are nuisances and vectors of pathogens between and among humans and livestock. Population suppression has been accomplished for decades with pyrethroids and acetylcholinesterase (AChE) inhibitors, but recurrent selection has led to increased frequency of alleles conferring resistance to those two classes of active ingredients (Geden et al., 2021). A common mechanism of resistance to both classes involves an altered target site (mutations in Voltage gated sodium channel (Vgsc) for pyrethroids or in Ace for AChE inhibitors). As part of ongoing efforts to understand the origin, spread and evolution of insecticide resistance alleles in house fly populations, we sampled flies in 11 different US states, sequenced, and then estimated frequencies of the Vgsc and Ace alleles. There was substantial variation in frequencies of the four common knockdown resistance alleles (kdr (L1014F), kdr-his (L1014H), super-kdr (M918T + L10414F) and 1B (T929I + L1014F) across the sampled states. The kdr allele was found in all 11 states and was the most common allele in four of them. The super-kdr allele was detected in only six collections, with the highest frequencies found in the north, northeast and central United States. The kdr-his allele was the most common allele in PA, NC, TN and TX. In addition, a novel super-kdr-like mutation in mutually exclusive exon 17a was found. The overall frequencies of the different Ace alleles, which we name based on the amino acid present at the mutation sites (V260L, A316S, G342A/V and F407Y), varied considerably between states. Five Ace alleles were identified: VAGF, VAVY, VAGY, VAAY and VSAY. Generally, the VSAY allele was the most common in the populations sampled. The susceptible allele (VAGF) was found in all populations, ranging in frequency from 3% (KS) to 41% (GA). Comparisons of these resistance allele frequencies with those previously found suggests a dynamic interaction between the different alleles, in terms of levels of resistance they confer and likely fitness costs they impose in the absence of insecticides.
ESTHER : Mertz_2023_Pestic.Biochem.Physiol_194_105497
PubMedSearch : Mertz_2023_Pestic.Biochem.Physiol_194_105497
PubMedID: 37532356
Gene_locus related to this paper: musdo-ACHE

Title : Common substitution mutation F348Y of acetylcholinesterase gene contributes to organophosphate and carbamate resistance in Cimex lectularius and C. hemipterus - Komagata_2021_Insect.Biochem.Mol.Biol__103637
Author(s) : Komagata O , Kasai S , Itokawa K , Minagawa K , Kazuma T , Mizutani K , Muto A , Tanikawa T , Adachi M , Komatsu N , Tomita T
Ref : Insect Biochemistry & Molecular Biology , :103637 , 2021
Abstract : Bed bug control highly depends on insecticides with a limited number of modes of action, especially since the global prevalence of pyrethroid resistance. De facto insecticide options against bed bugs in Japan are acetylcholinesterase inhibitors (AChEis) that consist of organophosphates and carbamates. However, the status of AChEi resistance and the mechanisms involved have not been ascertained. An amino acid substitution mutation, F348Y (or F331Y in standard numbering), occurring at an acyl-binding site of the paralogous AChE gene (p-Ace), was identified among AChEi-resistant colonies of both common and tropical bed bugs (Cimex lectularius and C. hemipterus, respectively). This mutation was genetically associated with propoxur and fenitrothion resistance in F348Y-segregating colonies of C. hemipterus. Inhibition of heterologously expressed C. lectularius p-Ace with insecticides revealed that the sensitivities of F348Y-carrying AChE decreased by orders of 10- to more than 100-fold for diazoxon, carbaryl, fenitroxon, paraoxon, chlorpyrifos-methyl, malaoxon, azamethiphos, methyl-paraoxon, and propoxur. In contrast, the mutant AChE showed a slightly decreased degree of sensitivity for dichlorvos and almost unchanged sensitivity for metoxadiazone. Further studies are needed to ascertain whether the practical efficacies of dichlorvos and metoxadiazone are ensured against F348Y-carrying bed bugs and whether other resistance mechanisms are involved.
ESTHER : Komagata_2021_Insect.Biochem.Mol.Biol__103637
PubMedSearch : Komagata_2021_Insect.Biochem.Mol.Biol__103637
PubMedID: 34454015
Gene_locus related to this paper: cimle-ACHE1

Title : Genome of the house fly, Musca domestica L., a global vector of diseases with adaptations to a septic environment - Scott_2014_Genome.Biol_15_466
Author(s) : Scott JG , Warren WC , Beukeboom LW , Bopp D , Clark AG , Giers SD , Hediger M , Jones AK , Kasai S , Leichter CA , Li M , Meisel RP , Minx P , Murphy TD , Nelson DR , Reid WR , Rinkevich FD , Robertson HM , Sackton TB , Sattelle DB , Thibaud-Nissen F , Tomlinson C , van de Zande L , Walden KK , Wilson RK , Liu N
Ref : Genome Biol , 15 :466 , 2014
Abstract : BACKGROUND: Adult house flies, Musca domestica L., are mechanical vectors of more than 100 devastating diseases that have severe consequences for human and animal health. House fly larvae play a vital role as decomposers of animal wastes, and thus live in intimate association with many animal pathogens. RESULTS: We have sequenced and analyzed the genome of the house fly using DNA from female flies. The sequenced genome is 691 Mb. Compared with Drosophila melanogaster, the genome contains a rich resource of shared and novel protein coding genes, a significantly higher amount of repetitive elements, and substantial increases in copy number and diversity of both the recognition and effector components of the immune system, consistent with life in a pathogen-rich environment. There are 146 P450 genes, plus 11 pseudogenes, in M. domestica, representing a significant increase relative to D. melanogaster and suggesting the presence of enhanced detoxification in house flies. Relative to D. melanogaster, M. domestica has also evolved an expanded repertoire of chemoreceptors and odorant binding proteins, many associated with gustation. CONCLUSIONS: This represents the first genome sequence of an insect that lives in intimate association with abundant animal pathogens. The house fly genome provides a rich resource for enabling work on innovative methods of insect control, for understanding the mechanisms of insecticide resistance, genetic adaptation to high pathogen loads, and for exploring the basic biology of this important pest. The genome of this species will also serve as a close out-group to Drosophila in comparative genomic studies.
ESTHER : Scott_2014_Genome.Biol_15_466
PubMedSearch : Scott_2014_Genome.Biol_15_466
PubMedID: 25315136
Gene_locus related to this paper: musdo-a0a1i8n2v5 , musdo-a0a1i8n5k8

Title : Overexpression of cytochrome P450 genes in pyrethroid-resistant Culex quinquefasciatus - Komagata_2010_Insect.Biochem.Mol.Biol_40_146
Author(s) : Komagata O , Kasai S , Tomita T
Ref : Insect Biochemistry & Molecular Biology , 40 :146 , 2010
Abstract : JPal-per strain of Culex quinquefasciatus exhibits extremely high resistance against pyrethroids in larvae, though the resistance is greatly lower in adults. Increased microsome monooxygenase metabolism is one of the major factors of the larval resistance in this strain. We cloned 46 novel cytochrome P450 cDNAs from JPal-per strain. An oligonucleotide microarray was designed for the novel 46 genes plus 16 previously reported P450 genes along with other non-P450 gene probes. Of these, five P450 genes were upregulated (>2.5-fold) in JPal-per larvae as compared with a susceptible strain. The expression ratios for the highest three among the five P450 genes screened in the microarray analysis, CYP9M10, CYP4H34 and CYP6Z10, were further validated by qPCR as 264-, 8.3-, and 3.9-fold, respectively. In JPal-per, the transcription levels of CYP9M10 and CYP4H34 showed a similar stage-dependent pattern as a high expression level during the larvfrom Ogasawara Islands in Japanal stage dramatically decreases in the adult stage. This larval specific overexpression manner of the two genes was consistent with the characteristic of stage-dependent resistance of JPal-per strain previously reported, suggesting that the two P450s, CYP9M10 and CYP4H34, are involved in pyrethroid detoxification in JPal-per strain.
ESTHER : Komagata_2010_Insect.Biochem.Mol.Biol_40_146
PubMedSearch : Komagata_2010_Insect.Biochem.Mol.Biol_40_146
PubMedID: 20080182
Gene_locus related to this paper: culqu-ACHE2

Title : PCR-based identification of Culex pipiens complex collected in Japan - Kasai_2008_Jpn.J.Infect.Dis_61_184
Author(s) : Kasai S , Komagata O , Tomita T , Sawabe K , Tsuda Y , Kurahashi H , Ishikawa T , Motoki M , Takahashi T , Tanikawa T , Yoshida M , Shinjo G , Hashimoto T , Higa Y , Kobayashi M
Ref : Jpn J Infect Dis , 61 :184 , 2008
Abstract : The Culex pipiens complex consists of vector mosquitoes that transmit important human pathogens. In this study we established a simplified method to distinguish three members of the Cx. pipiens complex, Cx. p. pallens Coquillet, Cx. p. form molestus Forskal, and Cx. quinquefasciatus Say, collected in Japan. Sequence analysis of the Drosophila Ace-orthologous acetylcholinesterase (Ace) gene (668 to 680 bp) revealed that a single polymorphic region characterizes each species. Based on this region, specific primers that distinguish Cx. p. form molestus (ACEpip2) and Cx. p. pallens (ACEpall2) were newly designed. Polymerase chain reactions were performed with the genomic DNA of Culex mosquitoes as the template, and these primers clearly distinguished two Culex spp. The accuracy of the designed primers was evaluated with 38 colonies of mosquito samples collected from 9 prefectures of Japan. The testing revealed that the distribution of anautogenous Cx. p. pipiens has not been confirmed in Japan. It also revealed that the male of Cx. p. pallens possesses an Ace gene haplotype that is highly similar to the sequence of Cx. quinquefasciatus. This improved method allows the evaluation of vector competence of Cx. p.molestus, which is the suspected vector of West Nile virus.
ESTHER : Kasai_2008_Jpn.J.Infect.Dis_61_184
PubMedSearch : Kasai_2008_Jpn.J.Infect.Dis_61_184
PubMedID: 18503166

Title : Antidiabetic and hypolipidemic effects of a novel dual peroxisome proliferator-activated receptor (PPAR) alpha\/gamma agonist, E3030, in db\/db mice and beagle dogs - Kasai_2008_J.Pharmacol.Sci_108_40
Author(s) : Kasai S , Inoue T , Yoshitomi H , Hihara T , Matsuura F , Harada H , Shinoda M , Tanaka I
Ref : J Pharmacol Sci , 108 :40 , 2008
Abstract : We investigated the antidiabetic effects of E3030, which is a potent dual activator of peroxisome proliferator-activated receptor (PPAR) alpha and PPARgamma, in an animal model of diabetes, C57BL/KsJ-db/db mice (db/db mice), and the lipidemic effects of E3030 in beagle dogs, whose PPARalpha and PPARgamma transactivation responses to E3030 were similar to those of humans. E3030 activated human PPARalpha, mouse PPARalpha, dog PPARalpha, human PPARgamma, mouse PPARgamma, and dog PPARgamma with EC(50) values of 65, 920, 87, 34, 73, and 34 nM, respectively, in the chimeric GAL4-PPAR receptor transactivation reporter assay. In db/db mice orally administered E3030 decreased blood glucose, triglyceride (TG), non-esterified fatty acids (NEFA), and insulin levels and increased blood adiponectin levels during a 14-day experimental period. Significant effects on blood glucose and adiponectin levels were observed at a dose of 3 mg/kg or greater. Furthermore, significant effects on blood TG, NEFA, and insulin levels were observed at doses of 1 mg/kg or more. An oral glucose tolerance test (OGTT) performed on Day 15 showed that E3030 at 3 mg/kg improved glucose tolerance in this model. Fourteen days of oral treatment with E3030 at a dose of 0.03 mg/kg or greater showed remarkable TG- and non high-density lipoprotein (non-HDL) cholesterol-lowering effects in beagle dogs. These results were similar to those observed for the PPARalpha agonist fenofibrate. E3030 also reduced apo C-III levels on Days 7 and 14, and elevated lipoprotein lipase (LPL) levels on Day 15. These results indicate that the TG- and non-HDL cholesterol-lowering actions of E3030 involve combined effects on reduction of apo C-III and elevation of LPL, resulting in increased lipolysis. The experimental results in animals suggest that E3030 has potential for use in the treatment of various aspects of metabolic dysfunction in type 2 diabetes, including dyslipidemia, hyperglycemia, hyperinsulinemia, and impaired glucose disposal.
ESTHER : Kasai_2008_J.Pharmacol.Sci_108_40
PubMedSearch : Kasai_2008_J.Pharmacol.Sci_108_40
PubMedID: 18776709

Title : Lateral transfer of the lux gene cluster - Kasai_2007_J.Biochem_141_231
Author(s) : Kasai S , Okada K , Hoshino A , Iida T , Honda T
Ref : J Biochem , 141 :231 , 2007
Abstract : The lux operon is an uncommon gene cluster. To find the pathway through which the operon has been transferred, we sequenced the operon and both flanking regions in four typical luminous species. In Vibrio cholerae NCIMB 41, a five-gene cluster, most genes of which were highly similar to orthologues present in Gram-positive bacteria, along with the lux operon, is inserted between VC1560 and VC1563, on chromosome 1. Because this entire five-gene cluster is present in Photorhabdus luminescens TT01, about 1.5 Mbp upstream of the operon, we deduced that the operon and the gene cluster were transferred from V. cholerae to an ancestor of Pr. luminescens. Because in both V. fischeri and Shewanella hanedai, luxR and luxI were found just upstream of the operon, we concluded that the operon was transferred from either species to the other. Because most of the genes flanking the operon were highly similar to orthologues present on chromosome 2 of vibrios, we speculated that the operon of most species is located on this chromosome. The undigested genomic DNAs of five luminous species were analysed by pulsed-field gel electrophoresis and Southern hybridization. In all the species except V. cholerae, the operons are located on chromosome 2.
ESTHER : Kasai_2007_J.Biochem_141_231
PubMedSearch : Kasai_2007_J.Biochem_141_231
PubMedID: 17169972
Gene_locus related to this paper: phopo-luxd , sheha-a0zsg8 , sheha-LUXD

Title : Molecular characterization of two acetylcholinesterase cDNAs in Pediculus human lice - Lee_2007_J.Med.Entomol_44_72
Author(s) : Lee SW , Kasai S , Komagata O , Kobayashi M , Agui N , Kono Y , Tomita T
Ref : Journal of Medical Entomology , 44 :72 , 2007
Abstract : Two cDNA sequences encoding Drosophila Ace-orthologous and -paralogous acetylcholinesterase precursors (AO- and AP-AChE precursors, respectively), were identified from the body louse, Pediculus humanus humanus L. In vitro inhibition studies with an insecticide-susceptible body louse strain exhibited a simplex inhibitory response of AChE. The I50 values of fenitroxon and carbaryl were estimated to be 2.2 and 1.9 microM for the susceptible lice, respectively. The mRNA level of AP-AChE gene was 3.1- and 9.3-fold higher than that of AO-AChE gene in the abdomen and the combined parts of the head and thorax, respectively, suggesting, due to its abundance, the potential significance of the AP-AChE isoform in Pediculus human lice in association with the efficacy of AChE-targeting pediculicides.
ESTHER : Lee_2007_J.Med.Entomol_44_72
PubMedSearch : Lee_2007_J.Med.Entomol_44_72
PubMedID: 17294923
Gene_locus related to this paper: pedhb-ACHE1 , pedhb-ACHE2 , pedhc-ACHE1 , pedhc-ACHE2

Title : Freshwater bioluminescence in Vibrio albensis (Vibrio cholerae biovar albensis) NCIMB 41 is caused by a two-nucleotide deletion in luxO - Kasai_2006_J.Biochem_139_471
Author(s) : Kasai S
Ref : J Biochem , 139 :471 , 2006
Abstract : We previously proposed that the function of the lux operon is to produce a halotolerant flavodoxin, FP390 or P-flavin binding protein, and not to produce light. A crucial basis of this hypothesis is that almost all species of luminous bacteria emit light in culture media containing over 2% NaCl. However, Vibrio albensis (Vibrio cholerae biovar albensis) NCIMB 41 emits light in freshwater and this appears to be in direct conflict with our hypothesis. To determine why this exceptional freshwater bioluminescence is emitted, we studied the lux operon and the regulatory system of the operon in this strain, and found that expression of the operon is regulated by a system involving a derivative of 4,5-dihydroxy 2,3-pentanedione, DPD, as an inducer, and the repressor gene for the lux operon, luxO, is damaged by deletion of two nucleotides. Furthermore, to study the effect of damage to the luxO gene, pUC18 derivatives containing the damaged and repaired luxO sequences were prepared. Cells transfected with the damaged luxO sequence emitted light like the parental strain, whereas ones transfected with the repaired one did so only sparingly. Here we show that the light emission in freshwater by this strain is not in conflict with our hypothesis.
ESTHER : Kasai_2006_J.Biochem_139_471
PubMedSearch : Kasai_2006_J.Biochem_139_471
PubMedID: 16567412
Gene_locus related to this paper: vibcl-q5ek34

Title : An amino acid substitution attributable to insecticide-insensitivity of acetylcholinesterase in a Japanese encephalitis vector mosquito, Culex tritaeniorhynchus - Nabeshima_2004_Biochem.Biophys.Res.Commun_313_794
Author(s) : Nabeshima T , Mori A , Kozaki T , Iwata Y , Hidoh O , Harada S , Kasai S , Severson DW , Kono Y , Tomita T
Ref : Biochemical & Biophysical Research Communications , 313 :794 , 2004
Abstract : A cDNA sequence encoding a Drosophila Ace-paralogous acetylcholinesterase (AChE) precursor of 701 amino acid residues was identified as the second AChE gene (Ace2) transcript from Culex tritaeniorhynchus. The Ace2 gene is tightly linked to organophosphorus insecticide (OP)-insensitivity of AChE on chromosome 2. The cDNA sequences were compared between an insecticide-susceptible strain and the resistant strain, TYM, that exhibits a 870-fold decrease in fenitroxon-sensitivity of AChE. Two amino acid substitutions were present in TYM mosquitoes. One is F455W whose homologous position in Torped AChE (Phe331) is located in the vicinity of the catalytic His in the acyl pocket of the active site gorge. The other substitution is located to a C-terminal Ile697 position that apparently seems to be excluded from the mature protein and is irrelevant to catalytic activity. The F455W replacement in the Ace2 gene is solely responsible for the insecticide-insensitivity of AChE in TYM mosquitoes.
ESTHER : Nabeshima_2004_Biochem.Biophys.Res.Commun_313_794
PubMedSearch : Nabeshima_2004_Biochem.Biophys.Res.Commun_313_794
PubMedID: 14697262
Gene_locus related to this paper: cultr-ACHE1 , cultr-ACHE2

Title : cDNA and deduced proteine sequence of acetylcholinesterase from the diamond back moth Plutella xylostella (L.) (Lepidoptera: Plutellidae) -
Author(s) : Ni XY , Tomita T , Kasai S , Kono Y
Ref : Appl Entomol Zool , 38 :49 , 2003
Gene_locus related to this paper: pluxy-ACHE