Mochizuki T

References (7)

Title : Omega-3 fatty acid epoxides are autocrine mediators that control the magnitude of IgE-mediated mast cell activation - Shimanaka_2017_Nat.Med_23_1287
Author(s) : Shimanaka Y , Kono N , Taketomi Y , Arita M , Okayama Y , Tanaka Y , Nishito Y , Mochizuki T , Kusuhara H , Adibekian A , Cravatt BF , Murakami M , Arai H
Ref : Nat Med , 23 :1287 , 2017
Abstract : Critical to the function of mast cells in immune responses including allergy is their production of lipid mediators, among which only omega-6 (omega-6) arachidonate-derived eicosanoids have been well characterized. Here, by employing comprehensive lipidomics, we identify omega-3 (omega-3) fatty acid epoxides as new mast cell-derived lipid mediators and show that they are produced by PAF-AH2, an oxidized-phospholipid-selective phospholipase A2. Genetic or pharmacological deletion of PAF-AH2 reduced the steady-state production of omega-3 epoxides, leading to attenuated mast cell activation and anaphylaxis following FcvarepsilonRI cross-linking. Mechanistically, the omega-3 epoxides promote IgE-mediated activation of mast cells by downregulating Srcin1, a Src-inhibitory protein that counteracts FcvarepsilonRI signaling, through a pathway involving PPARg. Thus, the PAF-AH2-omega-3 epoxide-Srcin1 axis presents new potential drug targets for allergic diseases.
ESTHER : Shimanaka_2017_Nat.Med_23_1287
PubMedSearch : Shimanaka_2017_Nat.Med_23_1287
PubMedID: 29035365
Gene_locus related to this paper: human-PAFAH2

Title : Draft Sequencing of the Heterozygous Diploid Genome of Satsuma (Citrus unshiu Marc.) Using a Hybrid Assembly Approach - Shimizu_2017_Front.Genet_8_180
Author(s) : Shimizu T , Tanizawa Y , Mochizuki T , Nagasaki H , Yoshioka T , Toyoda A , Fujiyama A , Kaminuma E , Nakamura Y
Ref : Front Genet , 8 :180 , 2017
Abstract : Satsuma (Citrus unshiu Marc.) is one of the most abundantly produced mandarin varieties of citrus, known for its seedless fruit production and as a breeding parent of citrus. De novo assembly of the heterozygous diploid genome of Satsuma ("Miyagawa Wase") was conducted by a hybrid assembly approach using short-read sequences, three mate-pair libraries, and a long-read sequence of PacBio by the PLATANUS assembler. The assembled sequence, with a total size of 359.7 Mb at the N50 length of 386,404 bp, consisted of 20,876 scaffolds. Pseudomolecules of Satsuma constructed by aligning the scaffolds to three genetic maps showed genome-wide synteny to the genomes of Clementine, pummelo, and sweet orange. Gene prediction by modeling with MAKER-P proposed 29,024 genes and 37,970 mRNA; additionally, gene prediction analysis found candidates for novel genes in several biosynthesis pathways for gibberellin and violaxanthin catabolism. BUSCO scores for the assembled scaffold and predicted transcripts, and another analysis by BAC end sequence mapping indicated the assembled genome consistency was close to those of the haploid Clementine, pummel, and sweet orange genomes. The number of repeat elements and long terminal repeat retrotransposon were comparable to those of the seven citrus genomes; this suggested no significant failure in the assembly at the repeat region. A resequencing application using the assembled sequence confirmed that both kunenbo-A and Satsuma are offsprings of Kishu, and Satsuma is a back-crossed offspring of Kishu. These results illustrated the performance of the hybrid assembly approach and its ability to construct an accurate heterozygous diploid genome.
ESTHER : Shimizu_2017_Front.Genet_8_180
PubMedSearch : Shimizu_2017_Front.Genet_8_180
PubMedID: 29259619
Gene_locus related to this paper: citsi-a0a067e7f4 , 9rosi-v4u6v9 , citsi-a0a067dhb0 , citsi-a0a067f614 , citun-a0a2h5ny34 , citsi-a0a067f6y7 , citcl-v4syg1

Title : Draft Genome Sequence of Weissella oryzae SG25T, Isolated from Fermented Rice Grains - Tanizawa_2014_Genome.Announc_2_e00667
Author(s) : Tanizawa Y , Fujisawa T , Mochizuki T , Kaminuma E , Suzuki Y , Nakamura Y , Tohno M
Ref : Genome Announc , 2 : , 2014
Abstract : Weissella oryzae was originally isolated from fermented rice grains. Here we report the draft genome sequence of the type strain of W. oryzae. This first report on the genomic sequence of this species may help identify the mechanisms underlying bacterial adaptation to the ecological niche of fermented rice grains.
ESTHER : Tanizawa_2014_Genome.Announc_2_e00667
PubMedSearch : Tanizawa_2014_Genome.Announc_2_e00667
PubMedID: 25013139
Gene_locus related to this paper: 9lact-a0a069d1e3

Title : Draft Genome Sequence of Lactobacillus oryzae Strain SG293T - Tanizawa_2014_Genome.Announc_2_e00861
Author(s) : Tanizawa Y , Fujisawa T , Mochizuki T , Kaminuma E , Nakamura Y , Tohno M
Ref : Genome Announc , 2 : , 2014
Abstract : We report the 1.86-Mb draft genome and annotation of Lactobacillus oryzae SG293(T) isolated from fermented rice grains. This genome information may provide further insights into the mechanisms underlying the fermentation of rice grains.
ESTHER : Tanizawa_2014_Genome.Announc_2_e00861
PubMedSearch : Tanizawa_2014_Genome.Announc_2_e00861
PubMedID: 25169865
Gene_locus related to this paper: 9laco-a0a081bkd2 , 9laco-a0a081bga0

Title : The forefront for novel therapeutic agents based on the pathophysiology of lower urinary tract dysfunction: pathophysiology of voiding dysfunction and pharmacological therapy - Takeda_2010_J.Pharmacol.Sci_112_121
Author(s) : Takeda M , Araki I , Mochizuki T , Nakagomi H , Kobayashi H , Sawada N , Zakohji H
Ref : J Pharmacol Sci , 112 :121 , 2010
Abstract : Normal lower urinary tract function consists of voiding and storage. During voiding, the pontine micturition reflex center orders the sacral parasympathetic nucleus to increase parasympathetic activity, resulting in urinary bladder detrusor contraction via activation of post-synaptic muscarinic receptors (M2/3) and in the relaxation of both urethral and prostatic smooth muscle by nitric oxide (NO). In addition, the rhabdosphincter relaxes by inhibition of the pudendal nucleus at the sacral portion. During the storage phase, increase in sympathetic activity relaxes the urinary bladder via activation of post-synaptic beta(3)-receptors and in the contraction of both urethral and prostatic smooth muscles via alpha(1)-adrenoceptor. Many factors influence voiding function, including lower urinary tract disorders (benign prostatic hyperplasia in males, urethral stricture) and neurological disorders (central and peripheral). Theories of pharmacotherapy for voiding dysfunction are 1) increase detrusor contractility and 2) decrease urethral resistance. The former includes agonists for muscarinic receptors and cholinesterase inhibitor; and the latter includes alpha(1)-adrenoceptor antagonists, NO donors, benzodiazepines, baclofen, dantrolene, and boturinum toxin.
ESTHER : Takeda_2010_J.Pharmacol.Sci_112_121
PubMedSearch : Takeda_2010_J.Pharmacol.Sci_112_121
PubMedID: 20134111

Title : In vitro metabolism of ciclesonide in human nasal epithelial cells - Sato_2007_Biopharm.Drug.Dispos_28_43
Author(s) : Sato H , Nave R , Nonaka T , Mochizuki T , Takahama S , Kondo S
Ref : Biopharmaceutics & Drug Disposition , 28 :43 , 2007
Abstract : Ciclesonide, a corticosteroid in development for allergic rhinitis, is converted to the pharmacologically active metabolite, desisobutyryl-ciclesonide (des-CIC), and des-CIC is subsequently esterified with fatty acids. Various experiments were performed to investigate ciclesonide metabolism in human nasal epithelial cells (HNEC). Human nasal epithelial cells were incubated with (a) 0.1 microM ciclesonide for 1 h and medium without ciclesonide for up to 24 h, (b) esterase inhibitors for 0.5 h followed by 5 microM ciclesonide for 6 h, or (c) 1 microM des-CIC for 6 h followed by medium without des-CIC for up to 24 h. Ciclesonide, des-CIC and des-CIC fatty acid conjugate concentrations were determined by high-performance liquid chromatography with tandem mass spectrometry. The amount of ciclesonide in HNEC decreased approximately 93-fold from 0.5 to 24 h. In contrast, des-CIC was present at constant levels throughout the post-treatment period. Furthermore, fatty acid conjugates of des-CIC were retained in HNEC up to 24 h post-treatment. Carboxylesterase and cholinesterase inhibitors decreased ciclesonide metabolism > or =50%. The total amounts of des-CIC fatty acid conjugates decreased and the extracellular amounts of des-CIC increased with time. In conclusion, ciclesonide was rapidly converted to des-CIC by carboxylesterases and cholinesterases, and des-CIC underwent reversible fatty acid conjugation in HNEC.
ESTHER : Sato_2007_Biopharm.Drug.Dispos_28_43
PubMedSearch : Sato_2007_Biopharm.Drug.Dispos_28_43
PubMedID: 17117454

Title : External monitoring of cerebral nicotinic acetylcholine receptors in living mice -
Author(s) : Liu X , Musachio JL , Wagner HN, Jr. , Mochizuki T , Dannals RF , London ED
Ref : Synapse , 27 :378 , 1997
PubMedID: 9372560