Family: AHL-acylase

Block: X

Parent Family: Abhydrolase_6


Bacterial AHL-acylases (acyl homoserine lactonase) (AiiO, AidH) from genus Ochrobactrum and related bacteria are different from previously described AHL-acylases such as PvdQ, QuiP (P.aeriginosa) HacA HacB (P.syringea which belong to Ntn-hydrolase superfamily Czajkowski et al.) and different from AHL-lactonase of B. thuringensis and A. tumefasciens (which are metallo-beta lactamases). AHL-acylase inactivates N-acyl homoserine lactone (AHL) quorum sensing signal molecules (lactonases are also inactivating enzymes). AHL-acylases could be useful for the control of development of infections caused by pathogenic bacteria and their persistence in respective hosts. The three fungi sequences are genes without real introns (horizontal transfer?). There are other N-acyl homoserine lactonase (AiiA) not alpha/beta hydroalses but Metallo-B-lactamases


Interpro : No interpro


Pdoc : No Pdoc

Pfam : PF12697 Abhydrolase_6

Prints : No Print

EC Number : No EC Number


Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
4G5X Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum 9rhiz-d2j2t6
4G8B Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum S102G mutant complexed with N-hexanoyl homoserine lactone 9rhiz-d2j2t6
4G8C Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum E219G mutant complexed with N-hexanoyl homoserine 9rhiz-d2j2t6
4G8D Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum S102G mutant 9rhiz-d2j2t6
4G9E Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum complexed with N-butanoyl homoserine 9rhiz-d2j2t6
4G9G Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum E219G mutant 9rhiz-d2j2t6

Gene_locus Name Species
9hyph-a0a256fxx7 Brucella thiophenivorans Alpha\/beta hydrolase Brucella thiophenivorans
9hyph-a0a2r3sty9 Pseudochrobactrum asaccharolyticum esterase pae07 Pseudochrobactrum asaccharolyticum
9brad-a3wre8 Nitrobacter sp. Nb-311A Alpha\/beta hydrolase fold protein Nitrobacter sp. Nb-311A
9rhiz-c4wkm9 Ochrobactrum intermedium LMG 3301 Abhydrolase domain-containing protein 8 Ochrobactrum intermedium LMG 3301
9rhiz-c8sqp9 Mesorhizobium opportunistum WSM2075 Alpha\/beta hydrolase fold protein Mesorhizobium opportunistum WSM2075
9rhiz-d2j2t6 Ochrobactrum sp. T63 AidH acyl homoserine lactonase Ochrobactrum sp. T63
9rhiz-d8x182 Ochrobactrum sp. A44 Alpha\/beta hydrolase fold protein Ochrobactrum sp. A44
agrvs-b9k312 Agrobacterium vitis (strain S4 \/ ATCC BAA-846) (Rhizobium vitis (strain S4)) Alpha\/beta hydrolase protein Agrobacterium vitis
mago7-a4rdm3 Magnaporthe oryzae (strain 70-15 \/ FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) Alpha\/beta hydrolase Magnaporthe oryzae
nithx-q1qq48 Nitrobacter hamburgensis (strain X14 \/ DSM 10229) alpha\/beta hydrolase fold Nitrobacter hamburgensis
nitwn-q3svd2 Nitrobacter winogradskyi (strain Nb-255 \/ ATCC 25391) alpha\/beta hydrolase fold Nitrobacter winogradskyi
ocha4-a6wx49 Ochrobactrum anthropi\; Ochrobactrum sp. AiiO-AIO6 N-acyl homoserine lactonase Ochrobactrum anthropi
olico-b6jdg5 Oligotropha carboxidovorans (strain ATCC 49405 \/ DSM 1227 \/ OM5) Alpha\/beta hydrolase fold protein Oligotropha carboxidovorans
parl1-a7hv92 Parvibaculum lavamentivorans (strain DS-1 \/ DSM 13023 \/ NCIMB 13966) Alpha\/beta hydrolase fold Parvibaculum lavamentivorans
pedhd-c6y3z2 Pedobacter heparinus (strain ATCC 13125 \/ DSM 2366 \/ NCIB 9290) Alpha\/beta hydrolase fold protein Pedobacter heparinus
penmq-b6qcs8 Penicillium marneffei (strain ATCC 18224 \/ CBS 334.59 \/ QM 7333) Valacyclovir hydrolase, putative Penicillium marneffei
phano-q0upq4 Phaeosphaeria nodorum (Septoria nodorum) putative uncharacterized protein Phaeosphaeria nodorum
pholu-PLU2437 Photorhabdus luminescens (subsp. laumondii) Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 \/ 3105-77) (Xenorhabdus luminescens (strain 2)) hydrolase Photorhabdus luminescens
rhoe4-c0ztk5 Rhodococcus erythropolis (strain PR4 \/ NBRC 100887) Putative hydrolase Rhodococcus erythropolis
rhoer-c3jn30 Rhodococcus erythropolis SK121 Hydrolase, alpha\/beta fold family Rhodococcus erythropolis SK121
rhop2-q2j0u2 Rhodopseudomonas palustris (strain HaA2) alpha\/beta hydrolase Rhodopseudomonas palustris
sacd2-q21ez0 Saccharophagus degradans (strain 2-40 \/ ATCC 43961 \/ DSM 17024) Hydrolases or acyltransferases (Alpha\/beta hydrolase superfamily)-like Saccharophagus degradans
talsn-b8ly90 Talaromyces stipitatus (strain ATCC 10500 \/ CBS 375.48 \/ QM 6759 \/ NRRL 1006) (Penicillium stipitatum) Alpha\/beta hydrolase, putative Talaromyces stipitatus
xenbs-d3v3f7 Xenorhabdus bovienii (strains SS-2004\; SS-2004) Putative Epoxide hydrolase Xenorhabdus bovienii

Substrate Chemical Nomenclature Proteins
N-Ac-DL-MetOMe methyl 2-acetamido-4-methylsulfanylbutanoate 9hyph-a0a256fxx7
(R,S)-DMPM methyl 2-(2,6-dimethylanilino)propanoate burce-lipaa    9hyph-a0a2r3sty9
C6-HSL N-[(3S)-2-oxooxolan-3-yl]hexanamide 9rhiz-d2j2t6    9bact-6EB3
3-oxo-C12-HSL 3-oxo-N-[(3S)-2-oxooxolan-3-yl]dodecanamide

Inhibitor Chemical Nomenclature Proteins
N-Hexanoyl-L-Homoserine (2S)-2-(hexanoylamino)-4-hydroxybutanoic acid 9rhiz-d2j2t6
N-butanoyl-L-homoserine (2S)-2-(butanoylamino)-4-hydroxybutanoic acid 9rhiz-d2j2t6

References (4)

Title : High-resolution structures of AidH complexes provide insights into a novel catalytic mechanism for N-acyl homoserine lactonase - Gao_2013_Acta.Crystallogr.D.Biol.Crystallogr_69_82
Author(s) : Gao A , Mei GY , Liu S , Wang P , Tang Q , Liu YP , Wen H , An XM , Zhang LQ , Yan XX , Liang DC
Ref : Acta Crystallographica D Biol Crystallogr , 69 :82 , 2013
Abstract :
PubMedSearch : Gao_2013_Acta.Crystallogr.D.Biol.Crystallogr_69_82
PubMedID: 23275166
Gene_locus related to this paper: 9rhiz-d2j2t6

Title : Inactivation of AHLs by Ochrobactrum sp. A44 depends on the activity of a novel class of AHL acylase - Czajkowski_2011_Environ.Microbiol.Rep_3_59
Author(s) : Czajkowski R , Krzyzanowska D , Karczewska J , Atkinson S , Przysowa J , Lojkowska E , Williams P , Jafra S
Ref : Environ Microbiol Rep , 3 :59 , 2011
Abstract :
PubMedSearch : Czajkowski_2011_Environ.Microbiol.Rep_3_59
PubMedID: 23761232
Gene_locus related to this paper: 9rhiz-d8x182

Title : AidH, an alpha\/beta-hydrolase fold family member from an Ochrobactrum sp. strain, is a novel N-acylhomoserine lactonase - Mei_2010_Appl.Environ.Microbiol_76_4933
Author(s) : Mei GY , Yan XX , Turak A , Luo ZQ , Zhang LQ
Ref : Applied Environmental Microbiology , 76 :4933 , 2010
Abstract :
PubMedSearch : Mei_2010_Appl.Environ.Microbiol_76_4933
PubMedID: 20525860
Gene_locus related to this paper: 9rhiz-d2j2t6

Title : Quenching of acyl-homoserine lactone-dependent quorum sensing by enzymatic disruption of signal molecules. - Czajkowski_2009_Acta.Biochim.Pol_56_1
Author(s) : Czajkowski R , Jafra S
Ref : Acta Biochim Pol , 56 :1 , 2009
Abstract :
PubMedSearch : Czajkowski_2009_Acta.Biochim.Pol_56_1
PubMedID: 19287806