Steryl_acetyl_hydrolase

Relationship

Family: Steryl_acetyl_hydrolase

Block: H

Parent Family: No family

Comment

Sterol homeostasis in eukaryotic cells relies on the reciprocal interconversion of free sterols and steryl esters. In Saccharomyces cerevisiae (Baker's yeast) sterol acetylation requires the acetyltransferase Atf2, whereas deacetylation requires Say1, a membrane-anchored deacetylase with a putative active site in the ER lumen. Lack of Say1 results in the secretion of acetylated sterols into the culture medium, indicating that the substrate specificity of Say1 determines whether acetylated sterols are secreted from the cells or whether they are deacetylated and retained. In S. cerevisiae cells lacking Say1 or Atf2 are sensitive against the plant-derived allylbenzene eugenol and both Say1 and Atf2 affect pregnenolone toxicity, indicating that lipid acetylation acts as a detoxification pathway (old DUF2424)

Database

Interpro : IPR019436 Steryl_acetyl_hydrolase Say1-like

PIRSF : No PIRSF

Pdoc : No Pdoc

Pfam : PF10340 Say1_Mug180

Prints : No Print

EC Number : No EC Number

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Gene_locus Name Species
9viru-a4l1y2 Gryllus bimaculatus nudivirus Putative uncharacterized protein Gryllus bimaculatus nudivirus
artoc-c5fwe0 Arthroderma otae (strain CBS 113480) (Microsporum canis) Alpha\/beta hydrolase fold protein Arthroderma otae
ashgo-q75a71 Ashbya gossypii (strain ATCC 10895 \/ CBS 109.51 \/ FGSC 9923 \/ NRRY-1056) (Yeast) (Eremothecium gossypii) ADR047Wp Ashbya gossypii
botfb-a6rm08 Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) Predicted protein Botryotinia fuckeliana
canal-q59l46 Candida albicans (Yeast) Potential esterase\/lipase Candida albicans
canal-q59nx4 Candida albicans (Yeast) Potential esterase\/lipase Candida albicans
canal-q59nx8 Candida albicans (Yeast) Potential esterase\/lipase Candida albicans
canal-q5a2u7 Candida albicans (Yeast) Potential esterase\/lipase Candida albicans
canal-q5ak09 Candida albicans (Yeast) Potential esterase\/lipase Candida albicans
canal-q5ak96 Candida albicans (Yeast) Potential esterase\/lipase Candida albicans
canaw-c4ykb1 Candida albicans (strain WO-1) (Yeast) Putative uncharacterized protein Candida albicans
canaw-c4yrn6 Candida albicans (strain WO-1) (Yeast) Putative uncharacterized protein Candida albicans
canaw-c4yrn9 Candida albicans (strain WO-1) (Yeast) Putative uncharacterized protein Candida albicans
canaw-c4yrr3 Candida albicans (Yeast) Putative uncharacterized protein Candida albicans
canaw-c4yrv3 Candida albicans (strain WO-1) (Yeast) Putative uncharacterized protein Candida albicans
canaw-c4ys26 Candida albicans (strain WO-1) (Yeast) Putative uncharacterized protein Candida albicans
candc-b9wa64 Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Esterase\/lipase, putative Candida dubliniensis
candc-b9wc93 Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Hydrolase, putative Candida dubliniensis
candc-b9whs3 Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Esterase\/lipase, putative Candida dubliniensis
candc-b9whs6 Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Esterase\/lipase, putative Candida dubliniensis
candc-b9whv2 Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Esterase\/lipase, putative Candida dubliniensis
candc-b9wi60 Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Esterase\/lipase, putative Candida dubliniensis
canga-q6ftv9 Candida glabrata (Yeast) (Torulopsis glabrata) Similar to uniprot|P53324 Saccharomyces cerevisiae YGR263c Candida glabrata
cantt-c5m5e8 Candida tropicalis (strain ATCC MYA-3404 \/ T1) (Yeast) Putative uncharacterized protein Candida tropicalis
cantt-c5mh80 Candida tropicalis (strain ATCC MYA-3404 \/ T1) (Yeast) Putative uncharacterized protein Candida tropicalis
cantt-c5mh89 Candida tropicalis (strain ATCC MYA-3404 \/ T1) (Yeast) Putative uncharacterized protein Candida tropicalis
cantt-c5mhh0 Candida tropicalis (strain ATCC MYA-3404 \/ T1) (Yeast) Putative uncharacterized protein Candida tropicalis
clal4-c4xy03 Clavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae) Putative uncharacterized protein Clavispora lusitaniae
clal4-c4yba6 Clavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae) Putative uncharacterized protein Clavispora lusitaniae
clal4-c4yba7 Clavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae) Putative uncharacterized protein Clavispora lusitaniae
copc7-a8nkc7 Coprinopsis cinerea (strain Okayama-7 \/ 130 \/ FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata) Putative uncharacterized protein Coprinopsis cinerea
debha-b5rue8 Debaryomyces hansenii (Yeast) (Torulaspora hansenii) DEHA2F11968p Debaryomyces hansenii
debha-b5rue9 Debaryomyces hansenii (Yeast) (Torulaspora hansenii) DEHA2F11990p Debaryomyces hansenii
fusox-w9ibd7 Fusarium oxysporum Uncharacterized protein Fusarium oxysporum FOSC 3-a
klula-q6cm49 Kluyveromyces lactis (Yeast) (Candida sphaerica) KLLA0E23013p Kluyveromyces lactis
lactc-c5dif7 Lachancea thermotolerans (strain CBS 6340) (Yeast) (Kluyveromyces thermotolerans) KLTH0E12166p Lachancea thermotolerans
lodel-a5duy7 Lodderomyces elongisporus (Yeast) (Saccharomyces elongisporus) Putative uncharacterized protein Lodderomyces elongisporus
lodel-a5e2s1 Lodderomyces elongisporus (Yeast) (Saccharomyces elongisporus) Putative uncharacterized protein Lodderomyces elongisporus
lodel-a5e2t8 Lodderomyces elongisporus (Yeast) (Saccharomyces elongisporus) Putative uncharacterized protein Lodderomyces elongisporus
lodel-a5e2v2 Lodderomyces elongisporus (Yeast) (Saccharomyces elongisporus) Putative uncharacterized protein Lodderomyces elongisporus
macph-k2ryd3 Macrophomina phaseolina (strain MS6) (Charcoal rot fungus) Carboxylesterase type B Macrophomina phaseolina
neucr-a7uwy7 Neurospora crassa (strain ATCC 24698 \/ 74-OR23-1A \/ CBS 708.71 \/ DSM 1257 \/ FGSC 987) hypothetical protein Neurospora crassa
penmq-b6qb64 Penicillium marneffei (strain ATCC 18224 \/ CBS 334.59 \/ QM 7333) Putative uncharacterized protein Penicillium marneffei
picgu-a5dc73 Pichia guilliermondii (Yeast) (Candida guilliermondii) Putative uncharacterized protein Pichia guilliermondii
picgu-a5dc74 Pichia guilliermondii (Yeast) (Candida guilliermondii) Putative uncharacterized protein Pichia guilliermondii
picgu-a5dc75 Pichia guilliermondii (Yeast) (Candida guilliermondii) Putative uncharacterized protein Pichia guilliermondii
picgu-a5dnr3 Pichia guilliermondii (Yeast) (Candida guilliermondii) Putative uncharacterized protein Pichia guilliermondii
picgu-a5drl3 Pichia guilliermondii (Yeast) (Candida guilliermondii) Putative uncharacterized protein Pichia guilliermondii
picst-a3ln06 Pichia stipitis (Yeast) Arylacetamide deacetylase Pichia stipitis
picst-a3lvi5 Pichia stipitis (Yeast) Hypothetical membrane protein Pichia stipitis
picst-a3lvu9 Pichia stipitis (Yeast) Predicted protein Pichia stipitis
podan-b2avm3 Podospora anserina Predicted CDS Pa_7_2450 Podospora anserina
pyrtr-b2w5w8 Pyrenophora tritici-repentis (strain Pt-1C-BFP) (Wheat tan spot fungus) (Drechslera tritici-repentis) Alpha\/beta hydrolase fold domain containing protein Pyrenophora tritici-repentis
pyrtr-b2wia5 Pyrenophora tritici-repentis (strain Pt-1C-BFP) (Wheat tan spot fungus) (Drechslera tritici-repentis) Putative uncharacterized protein Pyrenophora tritici-repentis
pyrtt-e3rgg7 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus)(Drechslera teres f. teres) Putative uncharacterized protein Pyrenophora teres f. teres
pyrtt-e3sa06 Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein Pyrenophora teres f. teres
schpo-SPBPB2B2.02 Schizosaccharomyces pombe (Fission yeast) and (strain ATCC 38366 \/ 972) Putative steryl acetyl hydrolase mug180 Meiotically up-regulated gene 180 protein Schizosaccharomyces pombe
scls1-a7el43 Sclerotinia sclerotiorum (strain ATCC 18683 \/ 1980 \/ Ss-1) (White mold) (Whetzelinia sclerotiorum) Predicted protein Sclerotinia sclerotiorum
talsn-b8m0h7 Talaromyces stipitatus (strain ATCC 10500 \/ CBS 375.48 \/ QM 6759 \/ NRRL 1006) (Penicillium stipitatum) Putative uncharacterized protein Talaromyces stipitatus
tubmm-d5glm4 Tuber melanosporum (strain Mel28) (Perigord black truffle) GSTUM_00010271001 Tuber melanosporum
vanpo-a7tnv7 Vanderwaltozyma polyspora (strain ATCC 22028 \/ DSM 70294) (Kluyveromyces polysporus) Putative uncharacterized protein Vanderwaltozyma polyspora
yarli-q6c493 Yarrowia lipolytica (Candida lipolytica) similar to wi|ncu07890.1 neurospora crassa ncu07890.1 Yarrowia lipolytica
yeast-SAY1 Saccharomyces cerevisiae (Baker's yeast) hypothetical 48.5da prot YG5J chromosome VII Saccharomyces cerevisiae
zygrc-c5dvl2 Zygosaccharomyces rouxii (strain ATCC 2623 \/ CBS 732 \/ IFO 1130 \/ NBR1623 \/ NCYC 568) (Candida mogii) ZYRO0D07590p Zygosaccharomyces rouxii

Gene_locus_frgt Name Species
monpe-e2l6w1 Moniliophthora perniciosa (strain FA553 \/ isolate CP02)(Witches'-broom disease fungus) (Marasmius perniciosus) Putative uncharacterized protein Moniliophthora perniciosa

Substrate Chemical Nomenclature Proteins
Cholesterol-acetate [(3S)-10,13-dimethyl-17-(6-methylheptan-2-yl)-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-yl] acetate

References (1)

Title : An acetylation\/deacetylation cycle controls the export of sterols and steroids from S. cerevisiae - Tiwari_2007_EMBO.J_26_5109
Author(s) : Tiwari R , Koffel R , Schneiter R
Ref : EMBO Journal , 26 :5109 , 2007
Abstract :
PubMedSearch : Tiwari_2007_EMBO.J_26_5109
PubMedID: 18034159
Gene_locus related to this paper: yeast-SAY1