Gene_Locus Report

Biblio print

Add to basket

Go to basket

Tree Display

AceDB Schema

XML Display

Feedback

Gene_locus Report for: 9zzzz-AncHLDRLuc

Synthetic construct of the common ancestor of haloalkane dehalogenases and Renilla luciferase

Comment
Luciferase from Renilla reniformis (RLuc) catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) Reconstruction of the ancestral enzyme shows it has both hydrolase and monooxygenase activities ( Chaloupkova et al.)


Relationship
Family|Haloalkane_dehalogenase-HLD2
Block| X
Position in NCBI Life Tree|synthetic construct
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> other sequences: N E > artificial sequences: N E > synthetic construct: N E


Molecular evidence
Database
No mutation
2 structures: 6G75, 6S6E
No kinetic





1 substrate:
Coelenterazine
No inhibitor
2 Structure : 6S6E, 6G75
Sequence
Graphical view for this peptide sequence: 9zzzz-AncHLDRLuc
Colored MSA for Haloalkane_dehalogenase-HLD2 (raw)
ATGDEWWAKCKQVDVLDSEMSYYDSDPGKHKNTVIFLHGNPTSSYLWRNV
IPHVEPLARCLAPDLIGMGKSGKLPNHSYRFVDHYRYLSAWFDSVNLPEK
VTIVCHDWGSGLGFHWCNEHRDRVKGIVHMESVVSPLKGWESFPETARDI
FQALRSEAGEEMVLKKNFFIERLLPSSIMRKLSEEEMDAYREPFVEPGES
RRPTLTWPREIPIKGDGPEDVIEIVKSYNKWLSTSKDIPKLFINADPGFF
SNAIKKVTKNWPNQKTVTVKGLHFLQEDSPEEIGEAIADFLNELTK
Legend This sequence has been compared to family alignement (MSA)
red => minority aminoacid
blue => majority aminoacid
color intensity => conservation rate
title => sequence position(MSA position)aminoacid rate
Catalytic site
Catalytic site in the MSA

ATGDEWWAKCKQVDVLDSEMSYYDSDPGKHKNTVIFLHGNPTSSYLWRNV
IPHVEPLARCLAPDLIGMGKSGKLPNHSYRFVDHYRYLSAWFDSVNLPEK
VTIVCHDWGSGLGFHWCNEHRDRVKGIVHMESVVSPLKGWESFPETARDI
FQALRSEAGEEMVLKKNFFIERLLPSSIMRKLSEEEMDAYREPFVEPGES
RRPTLTWPREIPIKGDGPEDVIEIVKSYNKWLSTSKDIPKLFINADPGFF
SNAIKKVTKNWPNQKTVTVKGLHFLQEDSPEEIGEAIADFLNELTK


Reference
    Title: Light-Emitting Dehalogenases: Reconstruction of Multifunctional Biocatalysts
    Chaloupkova, R, Liskova V, Tool M, Markova K, Sebestova E, Hernychova L, Marek M, Pinto GP, Pluskal D and Damborsky J <2 more author(s)>
    Ref: ACS Catal, 9:4810, 2019 : PubMed

            


Other Papers


Send your questions or comments to :
Mail to: Nicolas Lenfant, Thierry Hotelier, Yves Bourne, Pascale Marchot and Arnaud Chatonnet.
Please cite: Lenfant 2013 Nucleic.Acids.Res. or Marchot Chatonnet 2012 Prot.Pept Lett.
For technical information about these pages see:
ESTHER Home Page and ACEDB Home Page
AcePerl Lincoln Stein Home Page
webmaster

Acknowledgements and disclaimer