Winstanley_2009_Genome.Res_19_12

Reference

Title : Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool Epidemic Strain of Pseudomonas aeruginosa - Winstanley_2009_Genome.Res_19_12
Author(s) : Winstanley C , Langille MG , Fothergill JL , Kukavica-Ibrulj I , Paradis-Bleau C , Sanschagrin F , Thomson NR , Winsor GL , Quail MA , Lennard N , Bignell A , Clarke L , Seeger K , Saunders D , Harris D , Parkhill J , Hancock RE , Brinkman FS , Levesque RC
Ref : Genome Res , 19 :12 , 2009
Abstract :

Pseudomonas aeruginosa isolates have a highly conserved core genome representing up to 90% of the total genomic sequence with additional variable accessory genes, many of which are found in genomic islands or islets. The identification of the Liverpool Epidemic Strain (LES) in a children's cystic fibrosis (CF) unit in 1996 and its subsequent observation in several centers in the United Kingdom challenged the previous widespread assumption that CF patients acquire only unique strains of P. aeruginosa from the environment. To learn about the forces that shaped the development of this important epidemic strain, the genome of the earliest archived LES isolate, LESB58, was sequenced. The sequence revealed the presence of many large genomic islands, including five prophage clusters, one defective (pyocin) prophage cluster, and five non-phage islands. To determine the role of these clusters, an unbiased signature tagged mutagenesis study was performed, followed by selection in the chronic rat lung infection model. Forty-seven mutants were identified by sequencing, including mutants in several genes known to be involved in Pseudomonas infection. Furthermore, genes from four prophage clusters and one genomic island were identified and in direct competition studies with the parent isolate; four were demonstrated to strongly impact on competitiveness in the chronic rat lung infection model. This strongly indicates that enhanced in vivo competitiveness is a major driver for maintenance and diversifying selection of these genomic prophage genes.

PubMedSearch : Winstanley_2009_Genome.Res_19_12
PubMedID: 19047519
Gene_locus related to this paper: pseae-clipa , pseae-CPO , pseae-llipa , pseae-metx , pseae-PA0201 , pseae-PA0231 , pseae-PA0308 , pseae-PA0368 , pseae-PA0480 , pseae-PA0502 , pseae-PA0543 , pseae-PA0599 , pseae-PA1166 , pseae-PA1291 , pseae-PA1304 , pseae-PA1510 , pseae-PA1558 , pseae-PA1597 , pseae-PA1907 , pseae-PA2086 , pseae-PA2098 , pseae-PA2302 , pseae-PA2425 , pseae-PA2451 , pseae-PA2540 , pseae-PA2682 , pseae-PA2689 , pseae-PA2745 , pseae-PA2764 , pseae-PA2927 , pseae-PA2934 , pseae-PA2949 , pseae-PA3132 , pseae-PA3301 , pseae-PA3324 , pseae-PA3327 , pseae-PA3429 , pseae-PA3586 , pseae-PA3628 , pseae-PA3695 , pseae-PA3859 , pseae-PA3994 , pseae-PA4152 , pseae-PA4968 , pseae-PA5080 , pseae-PCHC , pseae-PCHF , pseae-PHAC2 , pseae-phaD , pseae-phag , pseae-Q9APW4 , pseae-rhla , pseae-q9i252

Related information

Gene_locus pseae-clipa    pseae-CPO    pseae-llipa    pseae-metx    pseae-PA0201    pseae-PA0231    pseae-PA0308    pseae-PA0368    pseae-PA0480    pseae-PA0502    pseae-PA0543    pseae-PA0599    pseae-PA1166    pseae-PA1291    pseae-PA1304    pseae-PA1510    pseae-PA1558    pseae-PA1597    pseae-PA1907    pseae-PA2086    pseae-PA2098    pseae-PA2302    pseae-PA2425    pseae-PA2451    pseae-PA2540    pseae-PA2682    pseae-PA2689    pseae-PA2745    pseae-PA2764    pseae-PA2927    pseae-PA2934    pseae-PA2949    pseae-PA3132    pseae-PA3301    pseae-PA3324    pseae-PA3327    pseae-PA3429    pseae-PA3586    pseae-PA3628    pseae-PA3695    pseae-PA3859    pseae-PA3994    pseae-PA4152    pseae-PA4968    pseae-PA5080    pseae-PCHC    pseae-PCHF    pseae-PHAC2    pseae-phaD    pseae-phag    pseae-Q9APW4    pseae-rhla    pseae-q9i252

Citations formats

Winstanley C, Langille MG, Fothergill JL, Kukavica-Ibrulj I, Paradis-Bleau C, Sanschagrin F, Thomson NR, Winsor GL, Quail MA, Lennard N, Bignell A, Clarke L, Seeger K, Saunders D, Harris D, Parkhill J, Hancock RE, Brinkman FS, Levesque RC (2009)
Newly introduced genomic prophage islands are critical determinants of in vivo competitiveness in the Liverpool Epidemic Strain of Pseudomonas aeruginosa
Genome Res 19 :12

Winstanley C, Langille MG, Fothergill JL, Kukavica-Ibrulj I, Paradis-Bleau C, Sanschagrin F, Thomson NR, Winsor GL, Quail MA, Lennard N, Bignell A, Clarke L, Seeger K, Saunders D, Harris D, Parkhill J, Hancock RE, Brinkman FS, Levesque RC (2009)
Genome Res 19 :12