NDRG2 is a member of the N-myc downstream regulated gene (NDRG) family, implicated in cell growth and differentiation. Investigation of NDRG2 molecular interactions by yeast two-hybrid screening identified prenylated Rab acceptor-1 (PRA1), involved in vesicle trafficking and protein transport, as binding partner. Binding of NDRG2 (and NDRG1-4) with PRA1 in vitro was confirmed by GST pull-down assay and immunoprecipitation, and colocalization was verified by confocal microscopy in HCT116 cells. Intracellular coexpression showed that NDRG2 and PRA1 synergistically downregulate T-cell factor (TCF) promoter activity and GSK3beta phosphorylation. Results suggest that NDRG2 and PRA1 might act synergistically to prevent signaling of TCF/beta-catenin.
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Kim JT, Kim JW, Kang YH, Kim KD, Lee SJ, Choi SC, Kim KS, Chae SK, Lim JS, Lee HG (2012) NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/beta-catenin signaling. FEBS Letters586: 3962-8
Kim JT, Kim JW, Kang YH, Kim KD, Lee SJ, Choi SC, Kim KS, Chae SK, Lim JS, Lee HG (2012) FEBS Letters586: 3962-8
