6YN2
Name : Crystal structure of Renilla reniformis luciferase variant RLuc8-W121F\/E144Q in complex with a coelenteramide (the postcatalytic enzyme-product complex)
Revelation date : 21-Apr-2021
Family : Haloalkane_dehalogenase-HLD2
Gene_locus : renre-luc
PDB file : ESTHER: header of PDB entry RCSB: Full entry
Comment
Schenkmayerova, A., Damborsky, J., Marek, M. Luciferase from Renilla reniformis (RLuc)<\/A> catalyzes the degradation of coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light (EC 1.13.12.5). This enzyme belongs to the Haloalkane dehalogenase family II with a different catalytic function (EC 3.8.1.5) Reconstruction of an ancestral enzyme shows it has both hydrolase and monooxygenase activities ( Chaloupkova et al.<\/A>) Preprint available of: Engineering Protein Dynamics of Ancestral Luciferase DOI: 10.26434\/chemrxiv.12808295
Ligand : Inhibitor Coelenteramide Ligand in structure: Ligplot
References (2)
| Title : Engineering the protein dynamics of an ancestral luciferase - Schenkmayerova_2021_Nat.Commun_12_3616 |
| Author(s) : Schenkmayerova A , Pinto GP , Toul M , Marek M , Hernychova L , Planas-Iglesias J , Daniel Liskova V , Pluskal D , Vasina M , Emond S , Dorr M , Chaloupkova R , Bednar D , Prokop Z , Hollfelder F , Bornscheuer UT , Damborsky J |
| Ref : Nat Commun , 12 :3616 , 2021 |
| Abstract : |
| PubMedSearch : Schenkmayerova_2021_Nat.Commun_12_3616 |
| PubMedID: 34127663 |
| Gene_locus related to this paper: renre-luc |
| Title : Engineering Protein Dynamics of Ancestral Luciferase - Schenkmayerova_2020_Chemrxiv__ |
| Author(s) : Schenkmayerova A , Pinto GP , Toul M , Marek M , Hernychova L , Planas-Iglesias J , Liskova V , Pluskal D , Vasina M , Emond S , Dorr M , Chaloupkova R , Bednar D , Prokop Z , Hollfelder F , Bornscheuer UT , Damborsky J |
| Ref : Chemrxiv , : , 2020 |
| Abstract : |
| PubMedSearch : Schenkmayerova_2020_Chemrxiv__ |
| PubMedID: |
| Gene_locus related to this paper: renre-luc |
Representative scheme of Prolylcarboxypeptidase structure and an image from PDBsum server
Databases
PDB-Sum : 6YN2 Previously Class, Architecture, Topology and Homologous superfamily - PDB-Sum server
FSSP : 6YN2 Fold classification based on Structure-Structure alignment of Proteins - FSSP server
SCOP : 6YN2 Structural Classification Of Protein - SCOP server
PROTEOPEDIA : 6YN2 3D, interactive encyclopedia of proteins - PROTEOPEDIA server