Aksoy S

References (5)

Title : Presence of extensive Wolbachia symbiont insertions discovered in the genome of its host Glossina morsitans morsitans - Brelsfoard_2014_PLoS.Negl.Trop.Dis_8_e2728
Author(s) : Brelsfoard C , Tsiamis G , Falchetto M , Gomulski LM , Telleria E , Alam U , Doudoumis V , Scolari F , Benoit JB , Swain M , Takac P , Malacrida AR , Bourtzis K , Aksoy S
Ref : PLoS Negl Trop Dis , 8 :e2728 , 2014
Abstract : Tsetse flies (Glossina spp.) are the cyclical vectors of Trypanosoma spp., which are unicellular parasites responsible for multiple diseases, including nagana in livestock and sleeping sickness in humans in Africa. Glossina species, including Glossina morsitans morsitans (Gmm), for which the Whole Genome Sequence (WGS) is now available, have established symbiotic associations with three endosymbionts: Wigglesworthia glossinidia, Sodalis glossinidius and Wolbachia pipientis (Wolbachia). The presence of Wolbachia in both natural and laboratory populations of Glossina species, including the presence of horizontal gene transfer (HGT) events in a laboratory colony of Gmm, has already been shown. We herein report on the draft genome sequence of the cytoplasmic Wolbachia endosymbiont (cytWol) associated with Gmm. By in silico and molecular and cytogenetic analysis, we discovered and validated the presence of multiple insertions of Wolbachia (chrWol) in the host Gmm genome. We identified at least two large insertions of chrWol, 527,507 and 484,123 bp in size, from Gmm WGS data. Southern hybridizations confirmed the presence of Wolbachia insertions in Gmm genome, and FISH revealed multiple insertions located on the two sex chromosomes (X and Y), as well as on the supernumerary B-chromosomes. We compare the chrWol insertions to the cytWol draft genome in an attempt to clarify the evolutionary history of the HGT events. We discuss our findings in light of the evolution of Wolbachia infections in the tsetse fly and their potential impacts on the control of tsetse populations and trypanosomiasis.
ESTHER : Brelsfoard_2014_PLoS.Negl.Trop.Dis_8_e2728
PubMedSearch : Brelsfoard_2014_PLoS.Negl.Trop.Dis_8_e2728
PubMedID: 24763283
Gene_locus related to this paper: 9rick-a0a059ivx6

Title : An insight into the sialome of Glossina morsitans morsitans - Alves-Silva_2010_BMC.Genomics_11_213
Author(s) : Alves-Silva J , Ribeiro JM , Van Den Abbeele J , Attardo G , Hao Z , Haines LR , Soares MB , Berriman M , Aksoy S , Lehane MJ
Ref : BMC Genomics , 11 :213 , 2010
Abstract : BACKGROUND: Blood feeding evolved independently in worms, arthropods and mammals. Among the adaptations to this peculiar diet, these animals developed an armament of salivary molecules that disarm their host's anti-bleeding defenses (hemostasis), inflammatory and immune reactions. Recent sialotranscriptome analyses (from the Greek sialo = saliva) of blood feeding insects and ticks have revealed that the saliva contains hundreds of polypeptides, many unique to their genus or family. Adult tsetse flies feed exclusively on vertebrate blood and are important vectors of human and animal diseases. Thus far, only limited information exists regarding the Glossina sialome, or any other fly belonging to the Hippoboscidae.
RESULTS: As part of the effort to sequence the genome of Glossina morsitans morsitans, several organ specific, high quality normalized cDNA libraries have been constructed, from which over 20,000 ESTs from an adult salivary gland library were sequenced. These ESTs have been assembled using previously described ESTs from the fat body and midgut libraries of the same fly, thus totaling 62,251 ESTs, which have been assembled into 16,743 clusters (8,506 of which had one or more EST from the salivary gland library). Coding sequences were obtained for 2,509 novel proteins, 1,792 of which had at least one EST expressed in the salivary glands. Despite library normalization, 59 transcripts were overrepresented in the salivary library indicating high levels of expression. This work presents a detailed analysis of the salivary protein families identified. Protein expression was confirmed by 2D gel electrophoresis, enzymatic digestion and mass spectrometry. Concurrently, an initial attempt to determine the immunogenic properties of selected salivary proteins was undertaken.
CONCLUSIONS: The sialome of G. m. morsitans contains over 250 proteins that are possibly associated with blood feeding. This set includes alleles of previously described gene products, reveals new evidence that several salivary proteins are multigenic and identifies at least seven new polypeptide families unique to Glossina. Most of these proteins have no known function and thus, provide a discovery platform for the identification of novel pharmacologically active compounds, innovative vector-based vaccine targets, and immunological markers of vector exposure.
ESTHER : Alves-Silva_2010_BMC.Genomics_11_213
PubMedSearch : Alves-Silva_2010_BMC.Genomics_11_213
PubMedID: 20353571
Gene_locus related to this paper: glomm-d3tlg8 , glomm-d3tm45 , glomm-d3tmt4 , glomm-d3tn19 , glomm-d3tp96 , glomm-d3tpx7 , glomm-d3tqi3 , glomm-d3tru7 , glomm-d3ts61 , glomm-q2pz00 , glomm-d3tpl7 , glomm-d3ts28 , glomm-d3tnu0

Title : Analysis of fat body transcriptome from the adult tsetse fly, Glossina morsitans morsitans - Attardo_2006_Insect.Mol.Biol_15_411
Author(s) : Attardo GM , Strickler-Dinglasan P , Perkin SA , Caler E , Bonaldo MF , Soares MB , El-Sayeed N , Aksoy S
Ref : Insect Molecular Biology , 15 :411 , 2006
Abstract : Tsetse flies (Diptera: Glossinidia) are vectors of pathogenic African trypanosomes. To develop a foundation for tsetse physiology, a normalized expressed sequence tag (EST) library was constructed from fat body tissue of immune-stimulated Glossina morsitans morsitans. Analysis of 20,257 high-quality ESTs yielded 6372 unique genes comprised of 3059 tentative consensus (TC) sequences and 3313 singletons (available at http:\/\/aksoylab.yale.edu). We analysed the putative fat body transcriptome based on homology to other gene products with known functions available in the public domain. In particular, we describe the immune-related products, reproductive function related yolk proteins and milk-gland protein, iron metabolism regulating ferritins and transferrin, and tsetse's major energy source proline biosynthesis. Expression analysis of the three yolk proteins indicates that all are detected in females, while only the yolk protein with similarity to lipases, is expressed in males. Milk gland protein, apparently important for larval nutrition, however, is primarily synthesized by accessory milk gland tissue.
ESTHER : Attardo_2006_Insect.Mol.Biol_15_411
PubMedSearch : Attardo_2006_Insect.Mol.Biol_15_411
PubMedID: 16907828
Gene_locus related to this paper: glomm-q2pz00

Title : Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host - Toh_2006_Genome.Res_16_149
Author(s) : Toh H , Weiss BL , Perkin SA , Yamashita A , Oshima K , Hattori M , Aksoy S
Ref : Genome Res , 16 :149 , 2006
Abstract : Sodalis glossinidius is a maternally transmitted endosymbiont of tsetse flies (Glossina spp.), an insect of medical and veterinary significance. Analysis of the complete sequence of Sodalis' chromosome (4,171,146 bp, encoding 2,432 protein coding sequences) indicates a reduced coding capacity of 51%. Furthermore, the chromosome contains 972 pseudogenes, an inordinately high number compared with that of other bacterial species. A high proportion of these pseudogenes are homologs of known proteins that function either in defense or in the transport and metabolism of carbohydrates and inorganic ions, suggesting Sodalis' degenerative adaptations to the immunity and restricted nutritional status of the host. Sodalis possesses three chromosomal symbiosis regions (SSR): SSR-1, SSR-2, and SSR-3, with gene inventories similar to the Type-III secretion system (TTSS) ysa from Yersinia enterolitica and SPI-1 and SPI-2 from Salmonella, respectively. While core components of the needle structure have been conserved, some of the effectors and regulators typically associated with these systems in pathogenic microbes are modified or eliminated in Sodalis. Analysis of SSR-specific invA transcript abundance in Sodalis during host development indicates that the individual symbiosis regions may exhibit different temporal expression profiles. In addition, the Sodalis chromosome encodes a complete flagella structure, key components of which are expressed in immature host developmental stages. These features may be important for the transmission and establishment of symbiont infections in the intra-uterine progeny. The data suggest that Sodalis represents an evolutionary intermediate transitioning from a free-living to a mutualistic lifestyle.
ESTHER : Toh_2006_Genome.Res_16_149
PubMedSearch : Toh_2006_Genome.Res_16_149
PubMedID: 16365377
Gene_locus related to this paper: sodgm-q2nqh6 , sodgm-q2nqt2 , sodgm-q2nss6 , sodgm-q2nt34 , sodgm-q2nth7 , sodgm-q2nue4 , sodgm-q2nvf1 , sodgm-q2nwd0

Title : Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia - Akman_2002_Nat.Genet_32_402
Author(s) : Akman L , Yamashita A , Watanabe H , Oshima K , Shiba T , Hattori M , Aksoy S
Ref : Nat Genet , 32 :402 , 2002
Abstract : Many insects that rely on a single food source throughout their developmental cycle harbor beneficial microbes that provide nutrients absent from their restricted diet. Tsetse flies, the vectors of African trypanosomes, feed exclusively on blood and rely on one such intracellular microbe for nutritional provisioning and fecundity. As a result of co-evolution with hosts over millions of years, these mutualists have lost the ability to survive outside the sheltered environment of their host insect cells. We present the complete annotated genome of Wigglesworthia glossinidia brevipalpis, which is composed of one chromosome of 697,724 base pairs (bp) and one small plasmid, called pWig1, of 5,200 bp. Genes involved in the biosynthesis of vitamin metabolites, apparently essential for host nutrition and fecundity, have been retained. Unexpectedly, this obligate's genome bears hallmarks of both parasitic and free-living microbes, and the gene encoding the important regulatory protein DnaA is absent.
ESTHER : Akman_2002_Nat.Genet_32_402
PubMedSearch : Akman_2002_Nat.Genet_32_402
PubMedID: 12219091
Gene_locus related to this paper: wigbr-BIOH