Fan Z

References (17)

Title : Insecticide Susceptibility and Detoxification Enzyme Activity of Frankliniella occidentalis under Three Habitat Conditions - Fan_2023_Insects_14_
Author(s) : Fan R , Fan Z , Sun Z , Chen Y , Gui F
Ref : Insects , 14 : , 2023
Abstract : Frankliniella occidentalis is a highly destructive and invasive agricultural pest that has developed resistance to a variety of insecticide classes. Different planting structures and insecticide use frequency can directly affect the resistance development of F. occidentalis. In this study, the susceptibility of three field strains of F. occidentalis, collected over one year (April to November) from three habitat conditions (facility agriculture area, FA; open field crop area, OF; agroforestry intersection area, AI), to spinetoram, spinosad, emamectin benzoate, chlorfenapyr, acetamiprid, and imidacloprid were monitored and compared. At the same time, the detoxification enzyme activity of F. occidentalis in different habitats was determined. The results showed that the susceptibility of the F. occidentalis population in FA was significantly lower than that of populations from OF and AI. Among them, the F. occidentalis population in FA had developed low levels of resistance to spinetoram (RR = 9.18-fold), emamectin benzoate (RR = 5.47-fold), chlorfenapyr (RR = 6.67-fold), and acetamiprid (RR = 7.49-fold), and had developed moderate level resistance to imidacloprid (RR = 11.67-fold), while still being relatively sensitive to spinosad. The population of F. occidentalis from OF had developed low level resistance to spinetoram (RR = 5.24-fold) but was still relatively sensitive to the other five insecticides. The resistance of F. occidentalis from AI to six insecticides was at relatively sensitive levels. The results of the enzyme activities of detoxification enzymes, including carboxylesterase (CarE), glutathione S-transferase (GST), acetylcholinesterase (AChE), and the cytochrome P450 enzyme system (CYP450), revealed that the activities of the FA population of F. occidentalis were significantly higher than those of the other two populations. The change of CarE activity in F. occidentalis was consistent with that of spinetoram resistance, indicating that CarE may be involved in the metabolic resistance of F. occidentalis to spinetoram. Among the three populations, the resistance and detoxification enzyme activities of F. occidentalis of the FA population to six insecticides were higher than those of the other two populations. Our findings, along with other strategies, are expected to help with the resistance management of F. occidentalis in different habitats.
ESTHER : Fan_2023_Insects_14_
PubMedSearch : Fan_2023_Insects_14_
PubMedID: 37504650

Title : Ambient 1,2-propanediol exposure accelerates the degradation of lipids and amino acids in milk via allosteric effects and affects the utilization of nutrients containing amide bond - Fan_2023_Food.Res.Int_170_112965
Author(s) : Fan Z , Jia W
Ref : Food Res Int , 170 :112965 , 2023
Abstract : The scandal of detecting 1, 2-propanediol (PL) in milk brought a crisis to the trust of consumers in the dairy industry, and the potential toxicity of PL has aroused the public concern about dietary exposure. A total of 200 pasteurized milk samples were collected from 15 regions, and the quantity of PL ranged between 0 and 0.31 g kg(-1). Pseudo-targeted quantitative metabolomics integrated with proteomics demonstrated that PL enhanced the reduction of kappa-casein, beta-casein, and 107 substances (41 amines and 66 amides) containing amide bonds. Pathway enrichment and topological analysis indicated that PL induced the metabolism of lipids, amino acids, oligosaccharide nucleotides, and alkaloids by accelerating the rate of nucleophilic reaction, and acetylcholinesterase, sarcosine oxidase, and prolyl 4-hydroxylase were determined as the vital enzymes related to the degradation of above nutrients. The results of molecular simulation calculation illustrated that the number of hydrogen bonds between acetylcholinesterase, sarcosine oxidase, and substrate increased to 2 and 3, respectively, while the position of hydrogen bonds between prolyl 4-hydroxylase and proline was shifted, indicating the change of conformation and the enhancement of hydrogen bond force were essential factors for the up-regulation of enzyme activity. This study first revealed the mechanism of deposition and transformation of PL in milk, which contributed to the knowledge of the quality control of milk and provided vital indicators to evaluate the adverse risks of PL in dairy products.
ESTHER : Fan_2023_Food.Res.Int_170_112965
PubMedSearch : Fan_2023_Food.Res.Int_170_112965
PubMedID: 37316053

Title : A feruloyl esterase\/cellulase integrated biological system for high-efficiency and toxic-chemical free isolation of tobacco based cellulose nanofibers - Zhao_2023_Carbohydr.Polym_313_120885
Author(s) : Zhao M , An X , Fan Z , Nie S , Cheng Z , Cao H , Zhang X , Mian MM , Liu H , Liu L
Ref : Carbohydr Polym , 313 :120885 , 2023
Abstract : Tobacco based cellulose nanofiber (TCNF) is a novel nanocellulose that has recently been used to replace undesirable wood pulp fibers in the preparation of reconstructed tobacco sheets (RTS). However, given the strict requirements for controlling toxic chemical content in tobacco products, there is a global interest in developing a green, efficient, and toxic-chemical free approach to isolate TCNF from tobacco stem as a bioresource. In this study, we propose a creative and environmentally friendly method to efficiently and safely isolate TCNF from tobacco stem pulp, which involves integrated biological pretreatment followed by a facile mechanical defibrillation process. Feruloyl esterase is used to pretreat the stem pulp by disrupting the ether and ester bonds between lignin and polysaccharide carbohydrates within the fiber wall, which effectively facilitates cellulase hydrolysis and swelling of the stem pulp fiber, as well as the following mechanical shearing treatment for TCNF isolation. The results demonstrate that TCNF obtained by the comprehensive feruloyl esterase/cellulase/mechanical process exhibit uniform and well-dispersed nanofiber morphology, higher crystallinity, and stronger mechanical properties than those of the control. The addition of 0.5 % TCNF can replace wood pulp by 18 wt% ~ 25 wt% in the production of RTS samples while maintaining their reasonable strength properties.
ESTHER : Zhao_2023_Carbohydr.Polym_313_120885
PubMedSearch : Zhao_2023_Carbohydr.Polym_313_120885
PubMedID: 37182973

Title : Long Short-Term Memory-Based Multiomics Reveal Lactobacillus casei-Derived Postbiotics Inhibiting Lipids Digestion via Mediating the Upregulation of alpha-Helices in Lipase - Fan_2023_Mol.Nutr.Food.Res__e2300336
Author(s) : Fan Z , Jia W
Ref : Mol Nutr Food Res , :e2300336 , 2023
Abstract : SCOPE: The antiobesity function of probiotics has been declared, while the application in high-risk patients and coding side effect has focused attention to postbiotics. This investigation profiles the mechanism of postbiotics affecting lipid digestion at molecular level, and establishes a momentous foundation for the clinical application of postbiotics in obesity suppression. METHODS AND RESULTS: An operational framework for butter digestion is constructed to collect the digests in the intestine at 0, 40, 80, and 120 min with various postbiotics supplement. A total of 227 lipids and 414 metabolites are detected by pseudo-targeted lipidomics integrated with the long short-term memory-based metabolomics, and the triacylglycerol (TG, from 134.1 to 184.7 mg kg(-1) ) and diacylglycerol (DG, from 4.2 to 8.4 mg kg(-1) ) are identified as significantly different lipids with or without postbiotics supplement. A total of eight substances related to the inhibition of gastric lipase and pancreatic lipase are screened through the molecular simulation computation in silicon and enzymatic reaction kinetics, and thus curtailing the bioaccessibility of lipids. CONCLUSIONS: Lactobacillus casei JCM1134-derived postbiotics propel the structure of lipase to aggregate by increasing the alpha-helix, and thus hampering the digestion of triglycerides through noncompetitive inhibition.
ESTHER : Fan_2023_Mol.Nutr.Food.Res__e2300336
PubMedSearch : Fan_2023_Mol.Nutr.Food.Res__e2300336
PubMedID: 37753826

Title : Long short-term memory based quasi-targeted lipidomics reveals propane-1,2-diol expediting the digestion of lipids via mediating the alpha-helices to a random curl or beta folding of lipase - Fan_2023_Food.Res.Int_173_113411
Author(s) : Fan Z , Jia W
Ref : Food Res Int , 173 :113411 , 2023
Abstract : Scandal of detecting 1,2-propanediol (PD) in milk brought a crisis to the trust of consumers in dairy industry, and investigations focused effect of PD on digestive behavior of milk were still restricted. Long short-term memory amalgamated to quasi-targeted lipidomics was applied to monitor dynamics changes of lipids during digestion and the pseudo-first-order kinetic model elucidated that PD elevated the digestibility of lipid with the degradation rate (S(-1)) ranged from 4440.31 to 5665.59 and mediated the transition of alpha-helices (26.46% to 19.07% of pancreatic lipase and 29.89% to 23.37% of gastric lipase) covering active center in lipase to random curl (48.25% to 51.17% of pancreatic lipase and 41.58% to 44.57% of gastric lipase) and beta folding (9.14% to 4.67% of pancreatic lipase and 6.52% to 10.05% of gastric lipase), ultimately upregulating the lipase activity and further intervening lipid nutrients utilization in milk. This study provided a critical insight about the impact of PD contamination at trace concentrations on the nutritional value of milk fat during digestion.
ESTHER : Fan_2023_Food.Res.Int_173_113411
PubMedSearch : Fan_2023_Food.Res.Int_173_113411
PubMedID: 37803749

Title : Rapid Screening of Lipase Inhibitors in Scutellaria baicalensis by Using Porcine Pancreatic Lipase Immobilized on Magnetic Core-Shell Metal-Organic Frameworks - Xu_2022_Molecules_27_3475
Author(s) : Xu J , Cao P , Fan Z , Luo X , Yang G , Qu T , Gao J
Ref : Molecules , 27 :3475 , 2022
Abstract : As for ligand fishing, the current immobilization approaches have some potential drawbacks such as the small protein loading capacity and difficult recycle process. The core-shell metal-organic frameworks composite (Fe(3)O(4)-COOH@UiO-66-NH(2)), which exhibited both magnetic characteristics and large specific surface area, was herein fabricated and used as magnetic support for the covalent immobilization of porcine pancreatic lipase (PPL). The resultant composite Fe(3)O(4)-COOH@UiO-66-NH(2)@PPL manifested a high loading capacity (247.8 mg/g) and relative activity recovery (101.5%). In addition, PPL exhibited enhanced tolerance to temperature and pH after immobilization. Then, the composite Fe(3)O(4)-COOH@UiO-66-NH(2)@PPL was incubated with the extract of Scutellaria baicalensis to fish out the ligands. Eight lipase inhibitors were obtained and identified by UPLC-Q-TOF-MS/MS. The feasibility of the method was further confirmed through an in vitro inhibitory assay and molecular docking. The proposed ligand fishing technique based on Fe(3)O(4)-COOH@UiO-66-NH(2)@PPL provided a feasible, selective, and effective platform for discovering enzyme inhibitors from natural products.
ESTHER : Xu_2022_Molecules_27_3475
PubMedSearch : Xu_2022_Molecules_27_3475
PubMedID: 35684413

Title : Salivary Abeta(1-42) may be a quick-tested biomarker for clinical use in Alzheimer's disease: a meta-analysis - Fan_2022_J.Neurol__
Author(s) : Fan Z , Li Z , Zhao S , Chen Y , Su Y , Peng G , Luo B
Ref : Journal of Neurology , : , 2022
Abstract : OBJECTIVE: Alzheimer's disease (AD) is the most prevalent form of dementia among the aging population. Cumulative studies aim to find non-invasive biomarkers in the early stages of AD. Saliva can be obtained easily, and salivary biomarkers have been proven effective in detecting neurodegenerative diseases. To find effective biomarkers in saliva and to help the diagnosis of AD, we performed a meta-analysis focusing on the salivary biomarkers (beta-amyloid 1-42 (Abeta(1-42)), total tau (t-tau), phosphorylated tau (p-tau) and acetylcholinesterase (AChE)) in AD. METHODS: We conducted a systematic online search for eligible studies reporting data on salivary biomarkers reflecting Abeta(1-42), t-tau, p-tau, and AChE in AD cohorts versus controls. Biomarkers' performance was assessed in a random-effects meta-analysis with the ratio of mean (RoM). RESULTS: A total of thirteen studies were included in the meta-analysis, of them seven involved salivary Abeta(1-42) (271 AD and 489 controls), five involved salivary t-tau (324 AD and 252 controls), four involved salivary p-tau (130 AD and 161 controls), and three involved salivary AChE (81 AD and 54 controls). AD showed significantly higher salivary Abeta(1-42) levels than control (ROM = 1.90 (95% CI 1.28-2.81, P = 0.001), while AD and control did not differ significantly on salivary t-tau, p-tau and AChE (ROM = 0.94, 95% CI 0.67-1.31, P = 0.72; ROM = 0.91, 95% CI 0.56-1.45, P = 0.68; ROM = 0.83, 95% CI 0.24-2.88, P = 0.77; respectively). CONCLUSION: The pooled results provide evidence that salivary Abeta(1-42) may serve as a sensitive biomarker for AD; nevertheless, larger AD cohorts are required to further confirm the sensitivity and specificity of salivary Abeta(1-42) for AD diagnosis.
ESTHER : Fan_2022_J.Neurol__
PubMedSearch : Fan_2022_J.Neurol__
PubMedID: 36562850

Title : Effects of elevated CO(2) on activities of protective and detoxifying enzymes in Frankliniella occidentalis and Frankliniella intonsa under spinetoram stress1 - Fan_2021_Pest.Manag.Sci__
Author(s) : Fan Z , Qian L , Chen Y , Fan R , He S , Gao Y , Gui F
Ref : Pest Manag Sci , : , 2021
Abstract : BACKGROUND: Elevated CO(2) can directly affect toxicity of insecticides to insects and the physiological response of insects to insecticides. Frankliniella occidentalis and Frankliniella intonsa are highly destructive pests that target horticultural crops. Spinetoram is an effective pesticide against thrips. This study sought to explore the effect of elevated CO(2) on efficacy of spinetoram against F. occidentalis and F. intonsa and effect of the spinetoram on activities of protective and detoxifying enzymes under elevated CO(2) . Notably, these enzymes can be exploited in further studies to develop interventions for thrips resistance management. RESULTS: Toxicity bioassay showed that the LC(50) values of F. occidentalis and F. intonsa exposed to spinetoram at elevated CO(2) (800 microL.L(-1) concentration) for 48 h was 0.08 and 0.006 mg.L(-1) , respectively, they are 0.62 and 0.75 times of those at ambient CO(2) (400 microL.L(-1) concentration). The findings showed that elevated CO(2) decreased activities of the superoxide dismutase and acetylcholinesterase in thrips, while increased the activities of carboxylesterase and glutathione-S-transferase. However, spinetoram increased activities of protective and detoxifying enzymes in both thrips under the two CO(2) levels. It has synergistic effect of elevated CO(2) and spinetoram treatment on the physiological enzyme activity of thrips, and the activities of analyzed enzymes were generally higher in F. occidentalis than those in F. intonsa. CONCLUSION: Elevated CO(2) amplifies the efficacy of spinetoram on thrips, F. intonsa is more susceptibility to spinetoram than F. occidentalis, and the latter showed better adaptation to adverse conditions than the former. This article is protected by copyright. All rights reserved.
ESTHER : Fan_2021_Pest.Manag.Sci__
PubMedSearch : Fan_2021_Pest.Manag.Sci__
PubMedID: 34480397

Title : A chromosome-level genome of the spider Trichonephila antipodiana reveals the genetic basis of its polyphagy and evidence of an ancient whole-genome duplication event - Fan_2021_Gigascience_10_
Author(s) : Fan Z , Yuan T , Liu P , Wang LY , Jin JF , Zhang F , Zhang ZS
Ref : Gigascience , 10 : , 2021
Abstract : BACKGROUND: The spider Trichonephila antipodiana (Araneidae), commonly known as the batik golden web spider, preys on arthropods with body sizes ranging from -2 mm in length to insects larger than itself (>20-50 mm), indicating its polyphagy and strong dietary detoxification abilities. Although it has been reported that an ancient whole-genome duplication event occurred in spiders, lack of a high-quality genome has limited characterization of this event. RESULTS: We present a chromosome-level T. antipodiana genome constructed on the basis of PacBio and Hi-C sequencing. The assembled genome is 2.29 Gb in size with a scaffold N50 of 172.89 Mb. Hi-C scaffolding assigned 98.5% of the bases to 13 pseudo-chromosomes, and BUSCO completeness analysis revealed that the assembly included 94.8% of the complete arthropod universal single-copy orthologs (n = 1,066). Repetitive elements account for 59.21% of the genome. We predicted 19,001 protein-coding genes, of which 96.78% were supported by transcriptome-based evidence and 96.32% matched protein records in the UniProt database. The genome also shows substantial expansions in several detoxification-associated gene families, including cytochrome P450 mono-oxygenases, carboxyl/cholinesterases, glutathione-S-transferases, and ATP-binding cassette transporters, reflecting the possible genomic basis of polyphagy. Further analysis of the T. antipodiana genome architecture reveals an ancient whole-genome duplication event, based on 2 lines of evidence: (i) large-scale duplications from inter-chromosome synteny analysis and (ii) duplicated clusters of Hox genes. CONCLUSIONS: The high-quality T. antipodiana genome represents a valuable resource for spider research and provides insights into this species' adaptation to the environment.
ESTHER : Fan_2021_Gigascience_10_
PubMedSearch : Fan_2021_Gigascience_10_
PubMedID: 33739402

Title : Inhibition of soluble epoxide hydrolase (sEH) protects hippocampal neurons and reduces cognitive decline in type 2 diabetic mice - Wu_2021_Eur.J.Neurosci__
Author(s) : Wu J , Fan Z , Zhao Y , Chen Q , Xiao Q
Ref : European Journal of Neuroscience , : , 2021
Abstract : Diabetes mellitus is a metabolic disorder that can lead to cognitive dysfunction. The hippocampus plays an important role in the cognitive function. Research has identified correlations between hippocampal impairment and diabetes, yet their intermediate remains unclear. Soluble epoxide hydrolase (sEH) is an enzyme that degrades epoxyeicosatrienoic acids (EETs), which have multiple protective effects by suppressing inflammation, apoptosis and oxidative stress. In this study, under diabetic conditions both hippocampal injury, and cognitive decline are accompanied by upregulation of sEH. Moreover, the sEH inhibitor trans-4-[4-(3-adamantan-1-y1-ureido)-cyclohexyloxy]-benzoic acid (t-AUCB) prevents cognitive dysfunction and decreased ROS accumulation and apoptosis in the diabetic hippocampus. t-AUCB treatment restored neuronal synaptic plasticity by restoring the expression of the postsynaptic proteins Postsynaptic density protein-95 (PSD95) and N-methyl-d-aspartate receptor subunit 2B (NR2B), the levels of which were positively correlated with Proline-rich tyrosine kinase 2 (Pyk2) levels under diabetic conditions. Thus, we suggest that hippocampal protection via sEH inhibition might be a potential therapeutic approach to attenuate the progression of cognitive decline in diabetes.
ESTHER : Wu_2021_Eur.J.Neurosci__
PubMedSearch : Wu_2021_Eur.J.Neurosci__
PubMedID: 33595911

Title : Soluble epoxide hydrolase inhibitor protects against blood-brain barrier dysfunction in a mouse model of type 2 diabetes via the AMPK\/HO-1 pathway - Wu_2020_Biochem.Biophys.Res.Commun__
Author(s) : Wu J , Zhao Y , Fan Z , Chen Q , Chen J , Sun Y , Jiang X , Xiao Q
Ref : Biochemical & Biophysical Research Communications , : , 2020
Abstract : Diabetes mellitus is a metabolic disorder that can lead to blood-brain barrier (BBB) disruption and cognitive decline. However, the mechanisms of BBB breakdown in diabetes are still unclear. Soluble epoxide hydrolase (sEH) is an enzyme that degrades epoxyeicosatrienoic acids (EETs), which have multiple protective effects on vascular structure and functions. In the current study, we showed increased vascular permeability of the BBB, which was accompanied by upregulation of sEH and downregulation of 14,15-EET. Moreover, the sEH inhibitor t-AUCB restored diabetic BBB integrity in vivo, and 14,15-EET prevented ROS accumulation and MEC injury in vitro. t-AUCB or 14,15-EET treatment provoked AMPK/HO-1 activation under diabetic conditions in vivo and in vitro. Thus, we suggest that decreased EET degradation by sEH inhibition might be a potential therapeutic approach to attenuate the progression of BBB injury in diabetic mice via AMPK/HO-1 pathway activation.
ESTHER : Wu_2020_Biochem.Biophys.Res.Commun__
PubMedSearch : Wu_2020_Biochem.Biophys.Res.Commun__
PubMedID: 32001002

Title : Therapeutic efficacy and immunoregulatory effect of Qiangji Jianli Capsule for patients with myasthenia gravis: Study protocol for a series of randomized, controlled N-of-1 trials - Weng_2020_Medicine.(Baltimore)_99_e23679
Author(s) : Weng S , Fan Z , Qiu G , Liu F , Huang L , Li J , Jiang X , Song Z , Gao Y , Zhong Z , He L , Kang L , Wu Y , Chen B , Jiang Q
Ref : Medicine (Baltimore) , 99 :e23679 , 2020
Abstract : INTRODUCTION: Myasthenia gravis (MG) is an autoimmune disease in which antibodies directly target components of the neuromuscular junction, causing neuromuscular conduction damage that leads to muscle weakness. The current pharmaceutical treatment for MG is still not ideal to address the problems of disease progression, high recurrence rate, and drug side effects. Clinical observations suggest that traditional Chinese medicine (TCM) can strengthen immunity and improve symptoms of MG patients, delay the progression of the disease, reduce or even prevent the need for immunosuppressive therapy when used in combination with acetylcholinesterase inhibitors or low-dose prednisone, as well as improve the quality of life of patients. The Qiangji Jianli Capsule (QJC) is a combination of medicinal herbs which is used in traditional Chinese medicine. Since MG is a rare disorder, randomized controlled trials comparing large cohorts are difficult to conduct. Therefore, we proposed to aggregate data from a small series of N-of-1 trials to assess the effect of the Chinese medical prescription QJC, which strengthens the spleen and nourishes Qi, as an add-on treatment for MG with spleen and stomach Qi deficiency syndrome. METHODS AND ANALYSIS: Single-center, randomized, double-blind, multiple crossover N-of-1 studies will compare QJC versus placebo in 5 adult MG patients with spleen and stomach Qi deficiency syndrome. Patients will undergo 3 cycles of two 4-week intervention periods. According to the treatment schedule, patients will continue to be treated with pyridine bromide tablets, prednisone acetate, tablets and/or tacrolimus capsules throughout the entire trial. Each period consisting of 4-week oral add-on treatment with QJC will be compared with 4-week add-on treatment with a placebo. The primary endpoints are quantitative myasthenia gravis (QMG) test; measurement of the amount of Treg cells and cytokines such as interferon-gamma (IFN-gamma), interleukin-4 (IL-4), interleukin-17A (IL-17A), and transforming growth factor-beta (TGF-beta); and corticosteroid or immunosuppressive agent dosage. Secondary outcome measures: Clinical: Evaluation of the effect of TCM syndromes; MG-activities of daily living (MG-ADL) scales; adverse events. ETHICS AND DISSEMINATION: This study was approved by The First Affiliated Hospital of Guangzhou University of Chinese Medicine (GZUCM), No. ZYYECK[2019]038. The results will be published in a peer-reviewed publication. Regulatory stakeholders will comment on the suitability of the trial for market authorization and reimbursement purposes. Trial registration: Chinese Clinical Trial Register, ID: ChiCTR2000033516. Registered on 3 June 2020, http://www.chictr.org.cn/showprojen.aspx?proj=54618.
ESTHER : Weng_2020_Medicine.(Baltimore)_99_e23679
PubMedSearch : Weng_2020_Medicine.(Baltimore)_99_e23679
PubMedID: 33371107

Title : Expression of DPP6 in Meckel's cartilage and tooth germs during mouse facial development - Du_2014_Biotech.Histochem_89_14
Author(s) : Du J , Fan Z , Ma X , Wu Y , Liu S , Gao Y , Shen Y , Fan M , Wang S
Ref : Biotech Histochem , 89 :14 , 2014
Abstract : Dipeptidyl peptidase-like protein 6 (DPP6), a member of the dipeptidyl aminopeptidase family, plays distinct roles in brain development, but its expression in embryonic Meckel's cartilage and tooth germs development is unknown. We analyzed the expression pattern of DPP6 in Meckel's cartilage and tooth germs development using in situ hybridization. DPP6 was detected in different patterns in Meckel's cartilage and tooth germs during mouse facial development from 11.5 to 13.5 days post-coitus (dpc) embryos. The expression pattern of DPP6 suggests that it may be involved in mandible and tooth development.
ESTHER : Du_2014_Biotech.Histochem_89_14
PubMedSearch : Du_2014_Biotech.Histochem_89_14
PubMedID: 23750656

Title : Enzyme-catalyzed degradation of biodegradable polymers derived from trimethylene carbonate and glycolide by lipases from Candida antarctica and Hog pancreas - Liu_2012_J.Biomater.Sci.Polym.Ed_23_1355
Author(s) : Liu F , Yang J , Fan Z , Li S , Kasperczyk J , Dobrzynski P
Ref : J Biomater Sci Polym Ed , 23 :1355 , 2012
Abstract : Enzyme-catalyzed degradation of poly(trimethylene carbonate) homo-polymer (PTMC) and poly(trimethylene carbonate-co-glycolide) co-polymer (PTGA) was investigated in the presence of lipases from Candida antarctica and Hog pancreas. Degradation was monitored by gravimetry, size-exclusion chromatography (SEC), nuclear magnetic resonance (NMR), tensiometry and environmental scanning electron microscopy (ESEM). PTMC can be rapidly degraded by Candida antarctica lipase with 98% mass loss after 9 days, while degradation by Hog pancreas lipase leads to 27% mass loss. Introduction of 16% glycolide units in PTMC chains strongly affects the enzymatic degradation. Hog pancreas lipase becomes more effective to PTGA co-polymer with a mass loss of 58% after 9 days, while Candida antarctica lipase seems not able to degrade PTGA. Bimodal molecular weight distributions are observed during enzymatic degradation of both PTMC and PTGA, which can be assigned to the fact that the surface is largely degraded while the internal part remains intact. The composition of the PTGA co-polymer remains constant, and ESEM shows that the polymers are homogeneously eroded during enzymatic degradation. Contact angle measurements confirm the enzymatic degradation mechanism, i.e., enzyme adsorption on the polymer surface followed by enzyme-catalyzed chain cleavage.
ESTHER : Liu_2012_J.Biomater.Sci.Polym.Ed_23_1355
PubMedSearch : Liu_2012_J.Biomater.Sci.Polym.Ed_23_1355
PubMedID: 21722422

Title : Expression of Dpp6 in mouse embryonic craniofacial development - Du_2011_Acta.Histochem_113_636
Author(s) : Du J , Fan Z , Ma X , Gao Y , Wu Y , Liu S , Shen Y , Fan M , Wang S
Ref : Acta Histochemica , 113 :636 , 2011
Abstract : Dipeptidyl-peptidase-like protein 6 (DPP6), a member of the dipeptidyl aminopeptidase family, plays distinct roles in brain development, but its expression in embryonic craniofacial development is unknown. The expression pattern of Dpp6 in the maxillofacial region during mouse embryonic craniofacial development was analyzed by whole-mount in situ hybridization on sections and by real-time PCR analysis. Dpp6 expression was detected during mouse embryonic craniofacial development in embryos 11-13.5 days post-coitum (dpc). Real-time PCR showed high Dpp6 expression present in 11.5-13.5dpc, and this then decreased as development of maxillofacial region progressed. The expression pattern of Dpp6 suggests that Dpp6 may be involved in embryonic craniofacial development.
ESTHER : Du_2011_Acta.Histochem_113_636
PubMedSearch : Du_2011_Acta.Histochem_113_636
PubMedID: 20817268

Title : Associations of PLA2G7 gene polymorphisms with plasma lipoprotein-associated phospholipase A2 activity and coronary heart disease in a Chinese Han population: the Beijing atherosclerosis study - Hou_2009_Hum.Genet_125_11
Author(s) : Hou L , Chen S , Yu H , Lu X , Chen J , Wang L , Huang J , Fan Z , Gu D
Ref : Hum Genet , 125 :11 , 2009
Abstract : The human PLA2G7 gene encodes lipoprotein-associated phospholipase A(2) (Lp-PLA(2)), an emerging risk factor for cardiovascular diseases. In the present study, seven single nucleotide polymorphisms (SNPs) in the PLA2G7 gene were genotyped in 827 patients with coronary heart disease (CHD), of which 512 were patients with myocardial infarction (MI), and 947 age- and gender-matched controls in a Chinese Han population. Plasma Lp-PLA(2) activity was measured in 416 randomly selected controls and 689 randomly selected CHD patients, including 423 MI patients. Lp-PLA(2) activity in CHD and MI cases was significantly higher (233.42+/-57.66 and 234.27+/-59.51 nmol ml(-1) min(-1), respectively) than in controls (211.47+/-58.61 nmol ml(-1) min(-1)). After adjusting for traditional risk factors by logistic regression, the odds ratios for CHD and MI per 1 standard deviation increment of Lp-PLA(2) activity were 1.27 (95% CI, 1.07-1.50) and 1.27 (95% CI, 1.05-1.54), respectively. Both single SNP analysis and haplotype analysis showed that the V279F and I198T polymorphisms were significantly associated with the reduced Lp-PLA(2) activity, but neither was associated with increased CHD risk. Both univariate and multivariate analyses, adjusting effects of conventional factors, indicated that the rs13210554 T allele increased the risk of MI in this Chinese Han population. In summary, an independent association of increased plasma Lp-PLA(2) activity with CHD and MI existed in this Chinese Han Population. Although V279F and I198T mutations significantly decreased the activity of Lp-PLA(2), only the promoter rs13210554 polymorphism was associated with MI. Lp-PLA(2) activity appears to influence the CHD and MI risk in Chinese Han population.
ESTHER : Hou_2009_Hum.Genet_125_11
PubMedSearch : Hou_2009_Hum.Genet_125_11
PubMedID: 19034521
Gene_locus related to this paper: human-PLA2G7

Title : Cloning, sequence analysis and expression of bacterial lipase-coding DNA fragments from environment in Escherichia coli - Fan_2009_Mol.Biol.Rep_36_1515
Author(s) : Fan Z , Yue C , Tang Y , Zhang Y
Ref : Mol Biol Rep , 36 :1515 , 2009
Abstract : Thirteen pairs of primers were designed, synthesized and used to clone the whole coding sequences or mature peptide-coding sequences of lipases. Bacteria producing extracellular lipases were enriched for the extraction of total DNAs. Eight fragments with 500-1,200 bp in length were obtained by using touchdown PCR and sequenced. Five of them were found to be lipase-coding DNAs. One fragment called BL9 that was 95.9% similar to a coding sequence of putative lipase. This lipase contained a Gly-His-Ser-Met-Gly motif which is matched to the consensus Gly-X-Ser-X-Gly conserved among lipolytic enzymes. The BL9 DNA fragment was inserted into the expression vector pET32a(+) of Escherichia coli. A functional product was yielded in the supernatant and produced a hydrolyzed zone on the tributyrin agar.
ESTHER : Fan_2009_Mol.Biol.Rep_36_1515
PubMedSearch : Fan_2009_Mol.Biol.Rep_36_1515
PubMedID: 18773306