Kawakami K

References (12)

Title : Effects of Smart Drugs on Cholinergic System and Non-Neuronal Acetylcholine in the Mouse Hippocampus: Histopathological Approach - Satoh_2022_J.Clin.Med_11_
Author(s) : Satoh R , Kawakami K , Nakadate K
Ref : J Clin Med , 11 : , 2022
Abstract : In recent years, people in the United States and other countries have been using smart drugs, called nootropic or cognitive enhancers, to improve concentration and memory learning skills. However, these drugs were originally prescribed for attention-deficit hyperactivity disorder and dementia, and their efficacy in healthy people has not yet been established. We focused on acetylcholine in the hippocampus, which is responsible for memory learning, and elucidate the long-term effects of smart drugs on the neural circuits. Smart drugs were administered orally in normal young mice for seven weeks. The hippocampus was sectioned and compared histologically by hematoxylin and eosin (HE) staining, immunohistochemistry for acetylcholine, and immunoelectron microscopy. There were no significant changes in acetylcholinesterase staining. However, in HE, we found perivascular edema, and choline acetyltransferase staining showed increased staining throughout the hippocampus and new signal induction in the perivascular area in the CA3, especially in the aniracetam and alpha-glyceryl phosphoryl choline group. Additionally, new muscarinic acetylcholine receptor signals were observed in the CA1 due to smart drug intake, suggesting that vasodilation might cause neuronal activation by increasing the influx of nutrients and oxygen. Moreover, these results suggest a possible new mechanism of acetylcholine-mediated neural circuit activation by smart drug intake.
ESTHER : Satoh_2022_J.Clin.Med_11_
PubMedSearch : Satoh_2022_J.Clin.Med_11_
PubMedID: 35743382

Title : Monolayer platform using human biopsy-derived duodenal organoids for pharmaceutical research - Yamashita_2021_Mol.Ther.Methods.Clin.Dev_22_263
Author(s) : Yamashita T , Inui T , Yokota J , Kawakami K , Morinaga G , Takatani M , Hirayama D , Nomoto R , Ito K , Cui Y , Ruez S , Harada K , Kishimoto W , Nakase H , Mizuguchi H
Ref : Mol Ther Methods Clin Dev , 22 :263 , 2021
Abstract : The human small intestine is the key organ for absorption, metabolism, and excretion of orally administered drugs. To preclinically predict these reactions in drug discovery research, a cell model that can precisely recapitulate the in vivo human intestinal monolayer is desired. In this study, we developed a monolayer platform using human biopsy-derived duodenal organoids for application to pharmacokinetic studies. The human duodenal organoid-derived monolayer was prepared by a simple method in 3-8 days. It consisted of polarized absorptive cells and had tight junctions. It showed much higher cytochrome P450 (CYP)3A4 and carboxylesterase (CES)2 activities than did the existing models (Caco-2 cells). It also showed efflux activity of P-glycoprotein (P-gp) and inducibility of CYP3A4. Finally, its gene expression profile was closer to the adult human duodenum, compared to the profile of Caco-2 cells. Based on these findings, this monolayer assay system using biopsy-derived human intestinal organoids is likely to be widely adopted.
ESTHER : Yamashita_2021_Mol.Ther.Methods.Clin.Dev_22_263
PubMedSearch : Yamashita_2021_Mol.Ther.Methods.Clin.Dev_22_263
PubMedID: 34485610

Title : Prescription of Antidementia Drugs and Antipsychotics for Elderly Patients in Japan: A Descriptive Study Using Pharmacy Prescription Data - Tsukada_2018_J.Geriatr.Psychiatry.Neurol__891988718785775
Author(s) : Tsukada T , Sato I , Matsuoka N , Imai T , Doi Y , Arai M , Fujii Y , Matsunaga T , Kawakami K
Ref : J Geriatr Psychiatry Neurol , :891988718785775 , 2018
Abstract : BACKGROUND: Antipsychotics are commonly used for managing behavioral and psychological symptoms of dementia among elderly patients with dementia receiving antidementia drugs (ADDs). However, the use of antipsychotics among these patients has not been investigated since 3 ADDs were approved in 2011 in Japan. METHOD: We conducted a descriptive study using pharmacy prescription data and identified patients aged >/=65 years who were newly prescribed donepezil, memantine, rivastigmine, and galantamine between January 1, 2012, and September 30, 2014. We determined the proportion of antipsychotic prescription and the factors affecting antipsychotic prescription using multivariable Cox proportional hazard models. RESULT: Of 13 876 patients, 1705 were memantine users, and the proportion of antipsychotic prescription among them was the highest (11.1%). Adjusted hazard ratios for donepezil, rivastigmine, and galantamine were 0.66, 0.56, and 0.66, respectively, relative to that for memantine. CONCLUSION: Compared to other ADD users, new memantine users were most likely to be prescribed antipsychotics.
ESTHER : Tsukada_2018_J.Geriatr.Psychiatry.Neurol__891988718785775
PubMedSearch : Tsukada_2018_J.Geriatr.Psychiatry.Neurol__891988718785775
PubMedID: 30016896

Title : Macrocarpal C isolated from Eucalyptus globulus inhibits dipeptidyl peptidase 4 in an aggregated form - Kato_2018_J.Enzyme.Inhib.Med.Chem_33_106
Author(s) : Kato E , Kawakami K , Kawabata J
Ref : J Enzyme Inhib Med Chem , 33 :106 , 2018
Abstract : Dipeptidyl peptidase 4 (DPP-4) inhibitors are used for the treatment of type-2 diabetes mellitus. Various synthetic inhibitors have been developed to date, and plants containing natural DPP-4 inhibitors have also been identified. Here, 13 plant samples were tested for their DPP-4 inhibitory activity. Macrocarpals A-C were isolated from Eucalyptus globulus through activity-guided fractionation and shown to be DPP-4 inhibitors. Of these, macrocarpal C showed the highest inhibitory activity, demonstrating an inhibition curve characterised by a pronounced increase in activity within a narrow concentration range. Evaluation of macrocarpal C solution by turbidity, nuclear magnetic resonance spectroscopy and mass spectrometry indicated its aggregation, which may explain the characteristics of the inhibition curve. These findings will be valuable for further study of potential small molecule DPP-4 inhibitors.
ESTHER : Kato_2018_J.Enzyme.Inhib.Med.Chem_33_106
PubMedSearch : Kato_2018_J.Enzyme.Inhib.Med.Chem_33_106
PubMedID: 29148282

Title : Enzymatic production of ferulic acid from defatted rice bran by using a combination of bacterial enzymes - Uraji_2013_Appl.Biochem.Biotechnol_171_1085
Author(s) : Uraji M , Kimura M , Inoue Y , Kawakami K , Kumagai Y , Harazono K , Hatanaka T
Ref : Appl Biochem Biotechnol , 171 :1085 , 2013
Abstract : Ferulic acid (FA), which is present in the cell walls of some plants, is best known for its antioxidant property. By combining a commercial enzyme that shows FA esterase activity with several Streptomyces carbohydrate-hydrolyzing enzymes, we succeeded in enhancing the enzymatic production of FA from defatted rice bran. In particular, the combination of three xylanases, an alpha-L-arabinofuranosidase, and an acetyl xylan esterase from Streptomyces spp. produced the highest increase in the amount of released FAs among all the enzymes in the Streptomyces enzymes library. This enzyme combination also had an effect on FA production from other biomasses, such as raw rice bran, wheat bran, and corncob.
ESTHER : Uraji_2013_Appl.Biochem.Biotechnol_171_1085
PubMedSearch : Uraji_2013_Appl.Biochem.Biotechnol_171_1085
PubMedID: 23512135

Title : Significant changes in the transesterification activity of free and mesoporous-immobilized Rhizopus oryzae lipase in ionic liquids - Shakeri_2010_J.Biotechnol_145_281
Author(s) : Shakeri M , Kawakami K
Ref : J Biotechnol , 145 :281 , 2010
Abstract : We examined the activity of free Rhizopus oryzae lipase (ROL) and ROL immobilized on mesoporous materials in transesterification reactions in various dialkylimidazolium-cation based ionic liquids. For free ROL, the highest activity (0.39 U/mg protein) was obtained in [OMIm][PF(6)] followed by that (0.28 U/mg protein) in [BMIm][PF(6)]. Specific activities of ROL immobilized on mesocellular foam (MCF) were only 0.47 and 0.43 U/mg protein in [OMIm][PF(6)] and [BMIm][PF(6)], respectively. However, the specific activities of ROL immobilized on octadecyl functionalized MCF (C(18)-MCF) increased significantly to 15.64 and 14.84 U/mg protein in [OMIm][PF(6)] and [BMIm][PF(6)], respectively. Consequently, ROL immobilized on C(18)-MCF is a promising biocatalyst for biotransformation reactions in ionic liquids.
ESTHER : Shakeri_2010_J.Biotechnol_145_281
PubMedSearch : Shakeri_2010_J.Biotechnol_145_281
PubMedID: 19948194

Title : Immobilization of Pseudomonas cepacia lipase onto electrospun polyacrylonitrile fibers through physical adsorption and application to transesterification in nonaqueous solvent - Sakai_2010_Biotechnol.Lett_32_1059
Author(s) : Sakai S , Liu Y , Yamaguchi T , Watanabe R , Kawabe M , Kawakami K
Ref : Biotechnol Lett , 32 :1059 , 2010
Abstract : The lipase of Pseudomonas cepacia was immobilized onto electrospun polyacrylonitrile (PAN) fibers and used for the conversion of (S)-glycidol with vinyl n-butyrate to glycidyl n-butyrate in isooctane. The rate of reaction with the adsorbed lipase was 23-fold higher than the initial material. After 10 recyclings, the initial reaction rate was 80% of the original rate. This system of enzyme immobilization is therefore suitable for carrying out transesterification reactions in nonaqueous solvents.
ESTHER : Sakai_2010_Biotechnol.Lett_32_1059
PubMedSearch : Sakai_2010_Biotechnol.Lett_32_1059
PubMedID: 20424890

Title : Production of butyl-biodiesel using lipase physically-adsorbed onto electrospun polyacrylonitrile fibers - Sakai_2010_Bioresour.Technol_101_7344
Author(s) : Sakai S , Liu Y , Yamaguchi T , Watanabe R , Kawabe M , Kawakami K
Ref : Bioresour Technol , 101 :7344 , 2010
Abstract : Butyl-biodiesel production using electrospun polyacrylonitrile fibers with Pseudomonas cepacia lipase immobilized through physical adsorption was studied. About 80% conversion to butyl-biodiesel was achieved after 24h by suspending the catalyst at 2.4 mg/mL in a mixture of rapeseed oil and n-butanol at a molar ratio of 1:3, containing water at 8000 ppm at 40 degrees C. A further 24h of operation resulted in 94% conversion. The initial reaction rate detected for this process was 65-fold faster than those detected for Novozym 435 on a total catalyst mass basis. The immobilized lipase continued to work as a catalyst for 27 d, within a 15% reduction in conversion yield at the outlet of the reactor compared with the average value detected during the first 3d of operation in a continuous butyl-biodiesel production system.
ESTHER : Sakai_2010_Bioresour.Technol_101_7344
PubMedSearch : Sakai_2010_Bioresour.Technol_101_7344
PubMedID: 20493682

Title : Transesterification by lipase entrapped in electrospun poly(vinyl alcohol) fibers and its application to a flow-through reactor - Sakai_2008_J.Biosci.Bioeng_105_687
Author(s) : Sakai S , Antoku K , Yamaguchi T , Kawakami K
Ref : J Biosci Bioeng , 105 :687 , 2008
Abstract : We entrapped lipase in electrospun poly(vinyl alcohol) fibers of approximately 1 mum in diameter and evaluated the transesterification activity by converting (s)-glycidol to glycidyl n-butyrate with vinyl n-butyrate. The initial transesterification rate of the entrapped lipase was 5.2-fold faster than that of non-treated lipase. The fibrous membrane could be used as a component of a flow-through reactor for continuous transesterification.
ESTHER : Sakai_2008_J.Biosci.Bioeng_105_687
PubMedSearch : Sakai_2008_J.Biosci.Bioeng_105_687
PubMedID: 18640613

Title : Development of a silica monolith microbioreactor entrapping highly activated lipase and an experiment toward integration with chromatographic separation of chiral esters - Kawakami_2007_J.Sep.Sci_30_3077
Author(s) : Kawakami K , Abe D , Urakawa T , Kawashima A , Oda Y , Takahashi R , Sakai S
Ref : J Sep Sci , 30 :3077 , 2007
Abstract : Microbioreactors are effective for high-throughput production of expensive products from small amounts of substrates. Lipases are versatile enzymes for chiral syntheses, and are highly activated when immobilized in alkyl-substituted silicates by the sol-gel method. For practical application of sol-gel immobilized lipases to a flow system, a microbioreactor loaded with a macroporous silica monolith is well suited, because it can be easily integrated with a chromatographic separator for optical resolution. We attempted to develop a microbioreactor containing a silica monolith-immobilized lipase. A nonshrinkable silica monolith was first formed from a 4:1 mixture of methyltrimethoxysilane (MTMS) and tetramethoxysilane (TMOS). It was then coated with silica precipitates entrapping lipase, derived from a 4:1 mixture of n-butyltrimethoxysilane (BTMS) and TMOS. As a result, monolith treated with the BTMS-based silicate entrapping lipase exhibited approximately ten times higher activity than nontreated monolith-immobilized lipase derived from the MTMS-based silicate, in transesterification between glycidol and vinyl n-butyrate in isooctane. A commercially available chiral column was connected in series to the monolith microbioreactor, and a pulse of substrate solution was supplied at the inlet of the reactor. Successful resolution of the racemic ester produced was achieved in the chromatographic column.
ESTHER : Kawakami_2007_J.Sep.Sci_30_3077
PubMedSearch : Kawakami_2007_J.Sep.Sci_30_3077
PubMedID: 17924370

Title : The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) - Gerhard_2004_Genome.Res_14_2121
Author(s) : Gerhard DS , Wagner L , Feingold EA , Shenmen CM , Grouse LH , Schuler G , Klein SL , Old S , Rasooly R , Good P , Guyer M , Peck AM , Derge JG , Lipman D , Collins FS , Jang W , Sherry S , Feolo M , Misquitta L , Lee E , Rotmistrovsky K , Greenhut SF , Schaefer CF , Buetow K , Bonner TI , Haussler D , Kent J , Kiekhaus M , Furey T , Brent M , Prange C , Schreiber K , Shapiro N , Bhat NK , Hopkins RF , Hsie F , Driscoll T , Soares MB , Casavant TL , Scheetz TE , Brown-stein MJ , Usdin TB , Toshiyuki S , Carninci P , Piao Y , Dudekula DB , Ko MS , Kawakami K , Suzuki Y , Sugano S , Gruber CE , Smith MR , Simmons B , Moore T , Waterman R , Johnson SL , Ruan Y , Wei CL , Mathavan S , Gunaratne PH , Wu J , Garcia AM , Hulyk SW , Fuh E , Yuan Y , Sneed A , Kowis C , Hodgson A , Muzny DM , McPherson J , Gibbs RA , Fahey J , Helton E , Ketteman M , Madan A , Rodrigues S , Sanchez A , Whiting M , Madari A , Young AC , Wetherby KD , Granite SJ , Kwong PN , Brinkley CP , Pearson RL , Bouffard GG , Blakesly RW , Green ED , Dickson MC , Rodriguez AC , Grimwood J , Schmutz J , Myers RM , Butterfield YS , Griffith M , Griffith OL , Krzywinski MI , Liao N , Morin R , Palmquist D , Petrescu AS , Skalska U , Smailus DE , Stott JM , Schnerch A , Schein JE , Jones SJ , Holt RA , Baross A , Marra MA , Clifton S , Makowski KA , Bosak S , Malek J
Ref : Genome Res , 14 :2121 , 2004
Abstract : The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline.
ESTHER : Gerhard_2004_Genome.Res_14_2121
PubMedSearch : Gerhard_2004_Genome.Res_14_2121
PubMedID: 15489334
Gene_locus related to this paper: human-AFMID , human-CES4A , human-CES5A , human-NOTUM , human-SERAC1 , human-SERHL2 , human-TMEM53 , mouse-acot1 , mouse-adcl4 , mouse-Ces2f , mouse-Ces4a , mouse-notum , mouse-q6wqj1 , mouse-Q9DAI6 , mouse-rbbp9 , mouse-SERHL , mouse-srac1 , mouse-tmm53 , rat-abhd6 , rat-abhda , rat-abhea , rat-abheb , rat-Ldah , rat-cd029 , rat-estd , rat-Kansl3 , rat-nceh1 , ratno-acph , ratno-CMBL , mouse-b2rwd2 , rat-b5den3 , rat-ab17c

Title : Insertional mutagenesis and rapid cloning of essential genes in zebrafish - Gaiano_1996_Nature_383_829
Author(s) : Gaiano N , Amsterdam A , Kawakami K , Allende M , Becker T , Hopkins N
Ref : Nature , 383 :829 , 1996
Abstract : Large-scale chemical mutagenesis screens in zebrafish have led to the isolation of thousands of lethal mutations in genes that are essential for embryonic development. However, the cloning of these mutated genes is difficult at present as it requires positional cloning methods. In Drosophila, chemical mutagenesis screens were complemented with P-element insertional mutagenesis which facilitated the cloning of many genes that had been identified by chemical lesions. To facilitate the cloning of vertebrate genes that are important during embryogenesis, we have developed an insertional mutagenesis strategy in zebrafish using a retroviral vector. Here, in a pilot screen of 217 proviral insertions, we obtained three insertional mutants with embryonic lethal phenotypes, and identified two of the disrupted genes. One of these, no arches, is essential for normal pharyngeal arch development, and is homologous to the recently characterized Drosophila zinc-finger gene, clipper, which encodes a novel type of ribonuclease. As it is easy to generate tens to hundreds of thousands of proviral transgenes in zebrafish, it should now be possible to use this screening method to mutate and then rapidly clone a large number of genes affecting vertebrate developmental and cellular processes.
ESTHER : Gaiano_1996_Nature_383_829
PubMedSearch : Gaiano_1996_Nature_383_829
PubMedID: 8893009