Mendoza J

References (4)

Title : Functional Annotation of ABHD14B, an Orphan Serine Hydrolase Enzyme - Rajendran_2020_Biochemistry_59_183
Author(s) : Rajendran A , Vaidya K , Mendoza J , Bridwell-Rabb J , Kamat SS
Ref : Biochemistry , 59 :183 , 2020
Abstract : The metabolic serine hydrolase family is, arguably, one of the largest functional enzyme classes in mammals, including humans, comprising 1-2% of the total proteome. This enzyme family uses a conserved nucleophilic serine residue in the active site to perform diverse hydrolytic reactions and consists of proteases, lipases, esterases, amidases, and transacylases, which are prototypical members of this family. In humans, this enzyme family consists of >250, of which approximately 40% members remain unannotated, in terms of both their endogenous substrates and the biological pathways that they regulate. The enzyme ABHD14B, an outlying member of this family, is also known as CCG1/TAFII250-interacting factor B, as it was found to be associated with transcription initiation factor TFIID. The crystal structure of human ABHD14B was determined more than a decade ago; however, its endogenous substrates remain elusive. In this paper, we annotate ABHD14B as a lysine deacetylase (KDAC), showing this enzyme's ability to transfer an acetyl group from a post-translationally acetylated lysine to coenzyme A (CoA), to yield acetyl-CoA, while regenerating the free amine of protein lysine residues. We validate these findings by in vitro biochemical assays using recombinantly purified human ABHD14B in conjunction with cellular studies in a mammalian cell line by knocking down ABHD14B and by identification of a putative substrate binding site. Finally, we report the development and characterization of a much-needed, exquisitely selective ABHD14B antibody, and using it, we map the cellular and tissue distribution of ABHD14B and prospective metabolic pathways that this enzyme might biologically regulate.
ESTHER : Rajendran_2020_Biochemistry_59_183
PubMedSearch : Rajendran_2020_Biochemistry_59_183
PubMedID: 31478652
Gene_locus related to this paper: human-CIB

Title : Caligus rogercresseyi acetylcholinesterase types and variants: a potential marker for organophosphate resistance - Agusti-Ridaura_2018_Parasit.Vectors_11_570
Author(s) : Agusti-Ridaura C , Dondrup M , Horsberg TE , Leong JS , Koop BF , Bravo S , Mendoza J , Kaur K
Ref : Parasit Vectors , 11 :570 , 2018
Abstract : BACKGROUND: Control of the sea louse Caligus rogercresseyi in the Chilean salmonid industry is reliant on chemical treatments. Azamethiphos was introduced in 2013, although other organophosphates were previously used. In 2014, reduced sensitivity to azamethiphos was detected in the Los Lagos Region using bioassays. The main target of organophosphates is the enzyme acetylcholinesterase (AChE). Mutations in the AChE gene are the main cause of organophosphate resistance in arthropods, including other sea lice. In the present study, we aimed to characterize C. rogercresseyi AChE(s) gene(s) and to study the association between AChE variants and azamethiphos resistance in this sea louse species. METHODS: Samples of adult male and female C. rogercresseyi were collected in the Los Lagos Region in 2014. Twenty-four hour exposure bioassays with azamethiphos were performed to select sensitive and resistant lice. The full-length cDNA coding sequences encoding for two AChEs in C. rogercresseyi were molecularly characterized. One of the AChE genes was screened by direct sequencing in the azamethiphos-selected lice to search for variants. An additional louse sampling was performed before and after an azamethiphos treatment in the field in 2017 to validate the findings. RESULTS: The molecular analysis revealed two putative AChEs in C. rogercresseyi. In silico analysis and 3D modelling of the protein sequences identified both of them as invertebrate AChE type 1; they were named C. rogercresseyi AChE1a and 1b. AChE1a had the characteristics of the main synaptic AChE, while AChE1b lacked some of the important amino acids of a typical AChE. A missense change found in the main synaptic AChE (1a), F318F/V (F290 in Torpedo californica), was associated with survival of C. rogercresseyi at high azamethiphos concentrations (bioassays and field treatment). The amino acid change was located in the acyl pocket of the active-site gorge of the protein. CONCLUSIONS: The present study demonstrates the presence of two types of AChE1 genes in C. rogercresseyi. Although enzymatic assays are needed, AChE1a is most probably the main synaptic AChE. The function of AChE1b is unknown, but evidence points to a scavenger role. The AChE1a F/V318 variant is most probably involved in organophosphate resistance, and can be a good marker for resistance monitoring.
ESTHER : Agusti-Ridaura_2018_Parasit.Vectors_11_570
PubMedSearch : Agusti-Ridaura_2018_Parasit.Vectors_11_570
PubMedID: 30376873
Gene_locus related to this paper: calro-ACHE1A , calro-ACHE1B

Title : Rev-Erbalpha modulates retinal visual processing and behavioral responses to light - Ait-Hmyed Hakkari_2016_FASEB.J_30_3690
Author(s) : Ait-Hmyed Hakkari O , Acar N , Savier E , Spinnhirny P , Bennis M , Felder-Schmittbuhl MP , Mendoza J , Hicks D
Ref : FASEB Journal , 30 :3690 , 2016
Abstract : The circadian clock is thought to adjust retinal sensitivity to ambient light levels, yet the involvement of specific clock genes is poorly understood. We explored the potential role of the nuclear receptor subfamily 1, group D, member 1 (REV-ERBalpha; or NR1D1) in this respect. In light-evoked behavioral tests, compared with wild-type littermates, Rev-Erbalpha(-/-) mice showed enhanced negative masking at low light levels (0.1 lx). Rev-Erbalpha(-/-) mouse retinas displayed significantly higher numbers of intrinsically photosensitive retinal ganglion cells (ipRGCs; 62% more compared with wild-type) and more intense melanopsin immunostaining of individual ipRGCs. In agreement with a pivotal role for melanopsin, negative masking at low light intensities was abolished in Rev-Erbalpha(-/-) Opn4(-/-) (melanopsin gene) double-null mice. Rev-Erbalpha(-/-) mice showed shortened latencies of both a and b electroretinogram waves, modified scotopic and photopic b-wave and scotopic threshold responses, and increased pupillary constriction, all of which suggested increased light sensitivity. However, wild-type and Rev-Erbalpha(-/-) mice displayed no detectable differences by in vivo fundus imaging, retinal histology, or expression of cell type-specific markers for major retinal cell populations. We conclude that REV-ERBalpha plays a major role in retinal information processing, and we speculate that REV-ERBalpha and melanopsin set sensitivity levels of the rod-mediated ipRGC pathway to coordinate activity with ambient light.
ESTHER : Ait-Hmyed Hakkari_2016_FASEB.J_30_3690
PubMedSearch : Ait-Hmyed Hakkari_2016_FASEB.J_30_3690
PubMedID: 27440795

Title : A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses - Hall_2005_Science_307_82
Author(s) : Hall N , Karras M , Raine JD , Carlton JM , Kooij TW , Berriman M , Florens L , Janssen CS , Pain A , Christophides GK , James K , Rutherford K , Harris B , Harris D , Churcher C , Quail MA , Ormond D , Doggett J , Trueman HE , Mendoza J , Bidwell SL , Rajandream MA , Carucci DJ , Yates JR, 3rd , Kafatos FC , Janse CJ , Barrell B , Turner CM , Waters AP , Sinden RE
Ref : Science , 307 :82 , 2005
Abstract : Plasmodium berghei and Plasmodium chabaudi are widely used model malaria species. Comparison of their genomes, integrated with proteomic and microarray data, with the genomes of Plasmodium falciparum and Plasmodium yoelii revealed a conserved core of 4500 Plasmodium genes in the central regions of the 14 chromosomes and highlighted genes evolving rapidly because of stage-specific selective pressures. Four strategies for gene expression are apparent during the parasites' life cycle: (i) housekeeping; (ii) host-related; (iii) strategy-specific related to invasion, asexual replication, and sexual development; and (iv) stage-specific. We observed posttranscriptional gene silencing through translational repression of messenger RNA during sexual development, and a 47-base 3' untranslated region motif is implicated in this process.
ESTHER : Hall_2005_Science_307_82
PubMedSearch : Hall_2005_Science_307_82
PubMedID: 15637271
Gene_locus related to this paper: plaba-q4ymx5 , plaba-q4ysr8 , plaba-q4ytp7 , plaba-q4yy11 , plaba-q4z0q9 , plaba-q4z5y0 , plaba-q4z5z8 , plaba-q4z215 , plach-q4x817 , plach-q4xb56 , plach-q4xbi1 , plach-q4xd64 , plach-q4xfc7 , plach-q4xm16 , plach-q4xmx8 , plach-q4xmy0 , plach-q4xsf9 , plach-q4xsg4 , plach-q4xsw6 , plach-q4xvc8 , plach-q4xxw0 , plach-q4xxy1 , plach-q4y0k9 , plach-q4y5u9 , plach-q4y6j0 , plach-q4y638 , plach-q4y740 , playo-PY05572 , playo-q7rq09