PHAZ7_phb_depolymerase
Relationship
Comment
Thermoalkalophilic hydrolase of Paucimonas and Shewanella species with high specificity for amorphous polyesters of short chain-length hydroxyalkanoic acids. An unusual substrate specificity for amorphous poly((R)-3-hydroxyalkanoates PHA and no homologies to any other PHB depolymerase. Formerly included in Lipase_2. Family IX was introduced by Handrick et al. (Arpigny_Jaeger Family IX). Paper: Arpigny and Jaeger (1999).
Database
Interpro : No interpro
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : No Pfam
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 2VTV | PhaZ7 depolymerase from Paucimonas lemoignei | psele-PHAZ7 |
| 2X5X | The crystal structure of PhaZ7 depolymerase from Paucimonas lemoignei at atomic (1.2 Angstrom) PhaZ7-SO2 complex | psele-PHAZ7 |
| 2X76 | The crystal structure of PhaZ7 depolymerase from Paucimonas lemoignei at atomic (1.2 Angstrom) resolution free PhaZ7 | psele-PHAZ7 |
| 4BRS | Structure of wild type PhaZ7 PHB depolymerase | psele-PHAZ7 |
| 4BTV | Structure of PhaZ7 PHB depolymerase in complex with 3HB trimer | psele-PHAZ7 |
| 4BVJ | Structure of Y105A mutant of PhaZ7 PHB depolymerase | psele-PHAZ7 |
| 4BVK | Structure of Y190E mutant of PhaZ7 PHB depolymerase | psele-PHAZ7 |
| 4BVL | Structure of 202-208 deletion mutant of PhaZ7 PHB depolymerase | psele-PHAZ7 |
| 4BYM | Structure of PhaZ7 PHB depolymerase Y105E mutant | psele-PHAZ7 |
| 5MLX | Open loop conformation of PhaZ7 Y105E mutant | psele-PHAZ7 |
| 5MLY | Closed loop conformation of PhaZ7 Y105E mutant | psele-PHAZ7 |
| Gene_locus | Name | Species |
|---|---|---|
| 9alte-a0a1i6i343 | Marinobacter daqiaonensis. Lipase (Class 2) | Marinobacter daqiaonensis |
| 9burk-a0a3a3g7n8 | Noviherbaspirillum sp.K1S02-23 . Lipase | Noviherbaspirillum sp. |
| 9burk-a0a3a3g7x6 | Noviherbaspirillum sp. Uncharacterized protein | Noviherbaspirillum sp. |
| 9burk-a0a3d6am77 | Oxalobacteraceae bacterium\; Noviherbaspirillum sp. K1S02-23. Uncharacterized protein | Oxalobacteraceae bacterium |
| 9delt-a0a2n2m1c4 | Deltaproteobacteria bacterium HGW-Deltaproteobacteria-1. Lipase | Deltaproteobacteria bacterium HGW-Deltaproteobacteria-1 |
| 9delt-a0a2w5tt48 | Archangium gephyra. Lipase | Archangium gephyra |
| 9fung-a0a261y1n4 | Bifiguratus adelaidae. Uncharacterized protein | Bifiguratus adelaidae |
| 9gamm-a0a0a0ez29 | Lysobacter daejeonensis GH1-9. Uncharacterized protein | Lysobacter daejeonensis GH1-9 |
| 9gamm-a0a0q7q3s1 | Lysobacter sp. Root494. Uncharacterized protein | Lysobacter sp. Root494 |
| 9gamm-a0a0q8dgr0 | Lysobacter sp. (Root604\; cf310). Uncharacterized protein | Lysobacter sp. Root604 |
| 9gamm-a0a0q8jfi3 | Lysobacter sp. Root690 Uncharacterized protein | Lysobacter sp. Root690 |
| 9gamm-a0a0q9eyk2 | Lysobacter sp. Uncharacterized protein | Lysobacter sp. |
| 9gamm-a0a0r0bs06 | Stenotrophomonas koreensis. Uncharacterized protein | Stenotrophomonas koreensis |
| 9gamm-a0a0r0cdn9 | Stenotrophomonas humi Uncharacterized protein | Stenotrophomonas humi |
| 9gamm-a0a0r0csd0 | Stenotrophomonas terrae\; Stenotrophomonas sp. Uncharacterized protein | Stenotrophomonas terrae |
| 9gamm-a0a0r0d0z9 | Pseudoxanthomonas dokdonensis. Uncharacterized protein | Pseudoxanthomonas dokdonensis |
| 9gamm-a0a0r0d468 | Stenotrophomonas ginsengisoli. Uncharacterized protein | Stenotrophomonas ginsengisoli |
| 9gamm-a0a0s2fcm5 | Lysobacter antibioticus PHB depolymerase PhaZ7 | Lysobacter antibioticus |
| 9gamm-a0a0s2g6c6 | Lysobacter gummosus. PHB depolymerase PhaZ7 | Lysobacter gummosus |
| 9gamm-a0a125u084 | Lysobacter capsici Uncharacterized protein | Lysobacter capsici |
| 9gamm-a0a126nk65 | Stenotrophomonas sp.\; Stenotrophomonas terrae Uncharacterized protein | Stenotrophomonas sp. KCTC 12332 |
| 9gamm-a0a1h3nn18 | Lysobacter sp. yr284 Uncharacterized protein | Lysobacter sp. yr284 |
| 9gamm-a0a1i4blf4 | Lysobacter sp. Uncharacterized protein | Lysobacter sp. |
| 9gamm-a0a1s9d3f3 | Chromatiales bacterium USCg_Taylor. Uncharacterized protein | Chromatiales bacterium USCg_Taylor |
| 9gamm-a0a1t4r8a4 | Lysobacter spongiicola DSM 21749. Lipase (Class 2) | Lysobacter spongiicola DSM 21749 |
| 9gamm-a0a291a2f2 | Lysobacter capsici\; Lysobacter sp.. Lipase | Lysobacter capsici |
| 9gamm-a0a2w6u1w4 | Stenotrophomonas sp Uncharacterized protein | Stenotrophomonas sp. |
| 9gamm-a0a371k2s7 | Lysobacter sp. zong2l5 Uncharacterized protein | Lysobacter sp. zong2l5 |
| lysen-a0a0s2dfs0 | Lysobacter enzymogenes\; Lysobacter sp. yr284. Uncharacterized protein | Lysobacter enzymogenes |
| lysen-a0a1h2zs61 | Lysobacter enzymogenes Uncharacterized protein | Lysobacter enzymogenes |
| psele-PHAZ7 |  Pseudomonas lemoignei PHB depolymerase PhaZ7 |
Pseudomonas lemoignei |
| rhofe-a0a1t1ayn0 | Rhodoferax fermentans. Uncharacterized protein | Rhodoferax fermentans |
| shehh-b0tls4 | Shewanella halifaxensis (strain HAW-EB4) Lipase class 2 | Shewanella halifaxensis |
| shesh-a8fz61 | Shewanella sediminis (strain HAW-EB3) Putative uncharacterized protein | Shewanella sediminis |
| Gene_locus_frgt | Name | Species |
|---|---|---|
| 9delt-a0a1v6a8g7 | Deltaproteobacteria bacterium ADurb.Bin151 Uncharacterized protein || Deltaproteobacteria bacterium ADurb.Bin151. Uncharacterized protein | Deltaproteobacteria bacterium ADurb.Bin151 |
| 9delt-a0a2d9tb81 | Sandaracinus sp Uncharacterized protein || Sandaracinus sp. Uncharacterized protein | Sandaracinus sp. |
| 9delt-a0a2n2fs65 | Deltaproteobacteria bacterium HGW-Deltaproteobacteria-5 Uncharacterized protein || Deltaproteobacteria bacterium HGW-Deltaproteobacteria-5. Lipase | Deltaproteobacteria bacterium HGW-Deltaproteobacteria-5 |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| P(3HO) | psefl-phaz pseac-q6ufw4 | |
| PHB | poly(3-hydroxybutyrate) | psesp-PHBDP1 aspfu-q4w9v8 crysp-Q874E9 alceu-phbc |
| RB3 | (3R)-3-[(3R)-3-[(3R)-3-hydroxybutanoyl]oxybutanoyl]oxybutanoic acid | psele-PHAZ7 penfu-PHAZ aspfu-q4w9v8 |
References (4)
| Title : The structure of PhaZ7 at atomic (1.2 A) resolution reveals details of the active site and suggests a substrate-binding mode - Wakadkar_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_648 |
| Author(s) : Wakadkar S , Hermawan S , Jendrossek D , Papageorgiou AC |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 66 :648 , 2010 |
| Abstract : |
| PubMedSearch : Wakadkar_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_648 |
| PubMedID: 20516591 |
| Gene_locus related to this paper: psele-PHAZ7 |
| Title : Structural basis of poly(3-hydroxybutyrate) hydrolysis by PhaZ7 depolymerase from Paucimonas lemoignei - Papageorgiou_2008_J.Mol.Biol_382_1184 |
| Author(s) : Papageorgiou AC , Hermawan S , Singh CB , Jendrossek D |
| Ref : Journal of Molecular Biology , 382 :1184 , 2008 |
| Abstract : |
| PubMedSearch : Papageorgiou_2008_J.Mol.Biol_382_1184 |
| PubMedID: 18706425 |
| Gene_locus related to this paper: psele-PHAZ7 |
| Title : Crystallization and preliminary X-ray analysis of a novel thermoalkalophilic poly(3-hydroxybutyrate) depolymerase (PhaZ7) from Paucimonas lemoignei - Kapetaniou_2005_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_61_479 |
| Author(s) : Kapetaniou EG , Braaz R , Jendrossek D , Papageorgiou AC |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 61 :479 , 2005 |
| Abstract : |
| PubMedSearch : Kapetaniou_2005_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_61_479 |
| PubMedID: 16511073 |
| Gene_locus related to this paper: psele-PHAZ7 |
| Title : A new type of thermoalkalophilic hydrolase of Paucimonas lemoignei with high specificity for amorphous polyesters of short chain-length hydroxyalkanoic acids - Handrick_2001_J.Biol.Chem_276_36215 |
| Author(s) : Handrick R , Reinhardt S , Focarete ML , Scandola M , Adamus G , Kowalczuk M , Jendrossek D |
| Ref : Journal of Biological Chemistry , 276 :36215 , 2001 |
| Abstract : |
| PubMedSearch : Handrick_2001_J.Biol.Chem_276_36215 |
| PubMedID: 11457823 |
| Gene_locus related to this paper: psele-PHAZ7 |