alceu-phbc
Alcaligenes eutrophus, Cupriavidus necator (Ralstonia eutropha) (polyhydroxyalkanoic acid synthase) phaC
Comment
catalytic triad C319, D480, and H508 || (freom uniprot) Forms a biodegradable plastic that is degraded naturally and completely by bacteria into carbon dioxide and water. Utilized in the medical industry. Plates made from PHA-based plastics can be left in place to help heal fractured bones. After the bone has healed, the plastic slowly breaks down in the body. Utilized by Imperial Chemical Industries (ICI) to produce a PHB-PHV (poly-B-valerate) copolymer sold under the trade name 'Biopol'. Biopol is used as packaging material. The PHB-PHV copolymer consists of approximately 20\% PHV and 80\% PHB. It can be synthesized by incorporating glucose and valeric acid into the medium. PHB-PHV is stronger and more flexible than regular PHB. Under industrial conditions, 80\% or higher of the cell dry weight of A.eutrophus usually consists of the PHB-PHV copolymer
Relationship
Family : PHA_synth_I
Block : X
Position in NCBI Life Tree : Ralstonia eutropha
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Proteobacteria N E > Betaproteobacteria N E > Burkholderiales N E > Burkholderiaceae N E > Cupriavidus N E > Cupriavidus necator N EMolecular evidence
Sequence
Peptide
MATGKGAAAS TQEGKSQPFK VTPGPFDPAT WLEWSRQWQG TEGNGHAAAS GIPGLDALAG VKIAPAQLGD IQQRYMKDFS ALWQAMAEGK AEATGPLHDR RFAGDAWRTN LPYRFAAAFY LLNARALTEL ADAVEADAKT RQRIRFAISQ WVDAMSPANF LATNPEAQRL LIESGGESLR AGVRNMMEDL TRGKISQTDE SAFEVGRNVA VTEGAVVFEN EYFQLLQYKP LTDKVHARPL LMVPPCINKY YILDLQPESS LVRHVVEQGH TVFLVSWRNP DASMAGSTWD DYIEHAAIRA IEVARDISGQ DKINVLGFCV GGTIVSTALA VLAARGEHPA ASVTLLTTLL DFADTGILDV FVDEGHVQLR EATLGGGAGA PCALLRGLEL ANTFSFLRPN DLVWNYVVDN YLKGNTPVPF DLLFWNGDAT NLPGPWYCWY LRHTYLQNEL KVPGKLTVCG VPVDLASIDV PTYIYGSRED HIVPWTAAYA STALLANKLR FVLGASGHIA GVINPPAKNK RSHWTNDALP ESPQQWLAGA IEHHGSWWPD WTAWLAGQAG AKRAAPANYG NARYRAIEPA PGRYVKAKA
References (10)
| Title : Structure and function of the N-terminal domain of Ralstonia eutropha polyhydroxyalkanoate synthase, and the proposed structure and mechanisms of the whole enzyme - Kim_2017_Biotechnol.J_12_1 |
| Author(s) : Kim YJ , Choi SY , Kim J , Jin KS , Lee SY , Kim KJ |
| Ref : Biotechnol J , 12 : , 2017 |
| Abstract : |
| PubMedSearch : Kim_2017_Biotechnol.J_12_1 |
| PubMedID: 27808475 |
| Gene_locus related to this paper: alceu-phbc |
| Title : Crystal structure of Ralstonia eutropha polyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms - Kim_2017_Biotechnol.J_12_2 |
| Author(s) : Kim J , Kim YJ , Choi SY , Lee SY , Kim KJ |
| Ref : Biotechnol J , 12 : , 2017 |
| Abstract : |
| PubMedSearch : Kim_2017_Biotechnol.J_12_2 |
| PubMedID: 27808482 |
| Gene_locus related to this paper: alceu-phbc |
| Title : Structure of the Catalytic Domain of the Class I Polyhydroxybutyrate Synthase from Cupriavidus necator - Wittenborn_2016_J.Biol.Chem_291_25264 |
| Author(s) : Wittenborn EC , Jost M , Wei Y , Stubbe J , Drennan CL |
| Ref : Journal of Biological Chemistry , 291 :25264 , 2016 |
| Abstract : |
| PubMedSearch : Wittenborn_2016_J.Biol.Chem_291_25264 |
| PubMedID: 27742839 |
| Gene_locus related to this paper: alceu-phbc |
| Title : Production of poly(3-hydroxypropionate) and poly(3-hydroxybutyrate-co-3-hydroxypropionate) from glucose by engineering Escherichia coli - Meng_2015_Metab.Eng_29_189 |
| Author(s) : Meng DC , Wang Y , Wu LP , Shen R , Chen JC , Wu Q , Chen GQ |
| Ref : Metab Eng , 29 :189 , 2015 |
| Abstract : |
| PubMedSearch : Meng_2015_Metab.Eng_29_189 |
| PubMedID: 25842374 |
| Gene_locus related to this paper: alceu-phbc |
| Title : Compositional regulation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by replacement of granule-associated protein in Ralstonia eutropha - Kawashima_2015_Microb.Cell.Fact_14_187 |
| Author(s) : Kawashima Y , Orita I , Nakamura S , Fukui T |
| Ref : Microb Cell Fact , 14 :187 , 2015 |
| Abstract : |
| PubMedSearch : Kawashima_2015_Microb.Cell.Fact_14_187 |
| PubMedID: 26597300 |
| Gene_locus related to this paper: alceu-phbc |
| Title : [Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose-utilizing strain of hydrogen-oxidizing bacteria, Ralstonia eutropha B8562] - Volova_2005_Mikrobiologiia_74_788 |
| Author(s) : Volova TG , Kozhevnikov IV , Dolgopolova Iu B , Trusova M , Kalacheva GS , Aref'eva Iu V |
| Ref : Mikrobiologiia , 74 :788 , 2005 |
| Abstract : |
| PubMedSearch : Volova_2005_Mikrobiologiia_74_788 |
| PubMedID: 16400989 |
| Gene_locus related to this paper: alceu-phbc |
| Title : In vitro polymerization and copolymerization of 3-hydroxypropionyl-CoA with the PHB synthase from Ralstonia eutropha - Song_2000_Biomacromolecules_1_433 |
| Author(s) : Song JJ , Zhang S , Lenz RW , Goodwin S |
| Ref : Biomacromolecules , 1 :433 , 2000 |
| Abstract : |
| PubMedSearch : Song_2000_Biomacromolecules_1_433 |
| PubMedID: 11710134 |
| Gene_locus related to this paper: alceu-phbc |
| Title : Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria - Steinbuchel_1992_FEMS.Microbiol.Rev_9_217 |
| Author(s) : Steinbuchel A , Hustede E , Liebergesell M , Pieper U , Timm A , Valentin H |
| Ref : FEMS Microbiology Reviews , 9 :217 , 1992 |
| Abstract : |
| PubMedSearch : Steinbuchel_1992_FEMS.Microbiol.Rev_9_217 |
| PubMedID: 1476773 |
| Gene_locus related to this paper: alceu-phbc , chrvi-phbc , pseol-phab , rhosh-PHAC |
| Title : Molecular analysis of the Alcaligenes eutrophus poly(3-hydroxybutyrate) biosynthetic operon: identification of the N terminus of poly(3-hydroxybutyrate) synthase and identification of the promoter - Schubert_1991_J.Bacteriol_173_168 |
| Author(s) : Schubert P , Kruger N , Steinbuchel A |
| Ref : Journal of Bacteriology , 173 :168 , 1991 |
| Abstract : |
| PubMedSearch : Schubert_1991_J.Bacteriol_173_168 |
| PubMedID: 1987116 |
| Gene_locus related to this paper: alceu-phbc |
| Title : Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC) - Peoples_1989_J.Biol.Chem_264_15298 |
| Author(s) : Peoples OP , Sinskey AJ |
| Ref : Journal of Biological Chemistry , 264 :15298 , 1989 |
| Abstract : |
| PubMedSearch : Peoples_1989_J.Biol.Chem_264_15298 |
| PubMedID: 2670936 |
| Gene_locus related to this paper: alceu-phbc |