Family: PHA_synth_III_C

Block: X

Parent Family: Abhydrolase_6


(The family Pha_synthase was split in four families (PHA_synth_I, PHA_synth_II, PHA_synth_III, PhaC_cen_dom ) according to Interpro and Tigrpfam. PhaC_cen_dom groups enzyme with the central domain but not the class I,II,III domains)This entry represents the PhaC subunit of a heterodimeric form of polyhydroxyalkanoic acid (PHA) synthase. Excepting the PhaC of Bacillus megaterium (which needs PhaR is not an a/b hydrolase), all members require PhaE (IPR010123 PhaE is not an a/b hydrolase) subunit for activity and are designated class III. (PhaC of Bacillus associated to PhaR are designed Class IV PHA_synth_IV but are not separated here) . This enzyme builds ester polymers for carbon and energy storage that accumulate in inclusions, and both this enzyme and the depolymerase associate with the inclusions. Class III enzymes polymerise short-chain-length hydroxyalkanoates. Poly-beta-hydroxybutyrate polymerase (PhaC) N-terminus. This entry represents the central domain of the bacterial poly-beta-hydroxybutyrate polymerase (PhaC). Polyhydroxyalkanoic acids (PHAs) are carbon and energy reserve polymers produced in some bacteria when carbon sources are plentiful and another nutrient, such as nitrogen, phosphate, oxygen, or sulphur, becomes limiting. PHAs composed of monomeric units ranging from 3 to 14 carbons exist in nature. When the carbon source is exhausted, PHA is utilised by the bacterium. PhaC links D-(-)-3-hydroxybutyrl-CoA to an existing PHA molecule by the formation of an ester bond PHA_synth_I TIGR01838 PHA_synth_II TIGR01839. The nucleophilic residue of the catalytic triad is a cysteine (with exceptions)


Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Gene_locus Name Species
desml-phaCDm Desulfococcus multivorans class III PHA synthase-like phaCDm Desulfococcus multivorans
9alte-a6f5f2 Marinobacter algicola DG893 Polyhydroxyalkanoate synthase Marinobacter algicola DG893
9arch-b3t6z4 uncultured marine crenarchaeote HF4000_APKG2O16 Putative alpha\/beta hydrolase fold uncultured marine crenarchaeote HF4000_APKG2O16
9baci-q2b2r4 Bacillus sp. NRRL B-14911 poly(r)-hydroxyalkanoic acid synthase, class III, phac subunit Bacillus sp. NRRL B-14911
9bact-b9xjh1 Pedosphaera parvula Ellin514 Alpha\/beta hydrolase fold protein Pedosphaera parvula Ellin514
9cren-Q8NKN4 uncultured crenarchaeote putative pha synthase uncultured crenarchaeote
9delt-a6ge99 Plesiocystis pacifica SIR-1 Acyl-CoA synthase Plesiocystis pacifica SIR-1
9eury-c1vc42 Halogeometricum borinquense DSM 11551 Poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit Halogeometricum borinquense DSM 11551
9firm-q3gbl4 Syntrophomonas wolfei subsp. wolfei str. Goettingen alpha\/beta hydrolase fold Syntrophomonas wolfei subsp. wolfei str. Goettingen
9gamm-a0y7n9 marine gamma proteobacterium HTCC2143 Probable poly-beta-hydroxybutyrate polymerase marine gamma proteobacterium HTCC2143
9gamm-a4bpm4 Nitrococcus mobilis Nb-231 Poly(3-hydroxybutyric acid) synthase Nitrococcus mobilis Nb-231
9gamm-b4wy97 Alcanivorax sp. DG881 Hydrolase, alpha\/beta fold family protein Alcanivorax sp. DG881
9gamm-b4x1v2 Alcanivorax sp. DG881 Hydrolase, alpha\/beta fold family protein Alcanivorax sp. DG881
9gamm-b5juw6 gamma proteobacterium HTCC5015 Polyhydroxyalkanoate synthase gamma proteobacterium HTCC5015
9gamm-b7ryc6 marine gamma proteobacterium HTCC2148 Hydrolase, alpha\/beta fold family protein marine gamma proteobacterium HTCC2148
9gamm-c0vkq2 Acinetobacter sp. ATCC 27244 Poly(R)-hydroxyalkanoic acid synthase Acinetobacter sp. ATCC 27244
9gamm-c0vnz2 Acinetobacter sp. ATCC 27244 Poly(R)-hydroxyalkanoic acid synthase Acinetobacter sp. ATCC 27244
9gamm-d0bx52 Acinetobacter sp. RUH2624 Poly(3-hydroxyalkanoate) synthetase Acinetobacter sp. RUH2624
9gamm-d0c4a7 Acinetobacter sp.\; Acinetobacter nosocomialis Poly(R)-hydroxyalkanoic acid synthase Acinetobacter sp. RUH2624
9gamm-q2bmp7 Oceanospirillum sp. MED92 poly(3-hydroxyalkanoate) synthase Oceanospirillum sp. MED92
9prot-a4u5l5 Magnetospirillum gryphiswaldense Poly-beta-hydroxybutyrate polymerase Magnetospirillum gryphiswaldense
9prot-a8u0q2 alpha proteobacterium BAL199 Poly-beta-hydroxybutyrate polymerase alpha proteobacterium BAL199
9sphn-q1ndz5 Sphingomonas sp. SKA58 Poly-beta-hydroxybutyrate polymerase Sphingomonas sp. SKA58
aciad-q6fa15 Acinetobacter sp. (strain ADP1) putative poly(r)-hydroxyalkanoic acid synthase (pha) Acinetobacter sp.
acibc-b2huf4 Acinetobacter baumannii\; Azomonas macrocytogenes, Poly(3-hydroxyalkanoate) synthetase class III Acinetobacter baumannii
acibc-b2i0a2 Acinetobacter baumannii (strains ACICU\; AYE\; AB307-0294\; AB0057\; ATCC 19606\; SDF\; ATCC17978\/NCDCKC755) Poly(3-hydroxyalkanoate) synthetase Acinetobacter baumannii
acica-d0s2a4 Acinetobacter calcoaceticus RUH2202 Poly(3-hydroxyalkanoate) synthetase Acinetobacter calcoaceticus RUH2202
acica-d0s3r3 Acinetobacter calcoaceticus RUH2202 Poly(3-hydroxyalkanoate) synthetase Acinetobacter calcoaceticus RUH2202
acicj-a5g0r8 Acidiphilium cryptum (strain JF-5) poly (3-hydroxybutyric acid) synthase Alpha\/beta hydrolase fold protein Acidiphilium cryptum
acijo-d0sek9 Acinetobacter johnsonii SH046 Putative uncharacterized protein Acinetobacter johnsonii SH046
aciju-d0sin4 Acinetobacter junii SH205 Poly(3-hydroxyalkanoate) synthetase Acinetobacter junii SH205
acilw-d0ssw2 Acinetobacter lwoffii SH145 Putative uncharacterized protein Acinetobacter lwoffii SH145
acira-c6rnl3 Acinetobacter radioresistens (strains SK82\; SH164) Poly(3-hydroxyalkanoate) synthetase Acinetobacter radioresistens SK82
alcbs-q0vmd6 Alcanivorax borkumensis (strain SK2 \/ ATCC 700651 \/ DSM 11573) Polyhydroxyalkanoate synthase Alcanivorax borkumensis
alcbs-q0vpn2 Alcanivorax borkumensis (strain SK2 \/ ATCC 700651 \/ DSM 11573) Polyhydroxyalkanoate synthase Alcanivorax borkumensis
alheh-q0a5r2 Alkalilimnicola ehrlichei (strain MLHE-1) Alpha\/beta hydrolase fold protein Alkalilimnicola ehrlichei
aroae-q5nz38 Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)) pha synthase subunit phac Aromatoleum aromaticum EbN1
azos1-d3nx23 Azospirillum sp. (strain B510) Polyhydroxyalkanoate synthase Azospirillum sp.
azovd-c1di03 Azotobacter vinelandii (strain DJ \/ ATCC BAA-1303) Poly(3-hydroxybutyrate) synthase subunit Azotobacter vinelandii
bacce-PHAC Bacillus cereus, Bacillus thuringiensis, Bacillus sp. Bacillus anthracis (strain Ames)pha synthase poly(r)-hydroxyalkanoic acid synthase, class III, phac subunit Bacillus cereus
bacce-q2ebc5 Bacillus cereus subsp. cytotoxis NVH 391-98 poly(r)-hydroxyalkanoic acid synthase, class III, phac subunit Bacillus cereus subsp. cytotoxis NVH 391-98
bacme-PHAC Bacillus megaterium pha synthase phac Bacillus megaterium
braja-BLR2885 Bradyrhizobium japonicum blr2885 protein Bradyrhizobium japonicum
censy-a0rx85 Cenarchaeum symbiosum (strain A) Poly(3-hydroxyalkanoate) synthetase Cenarchaeum symbiosum
chlaa-a9wiz2 Chloroflexus aurantiacus (strain ATCC 29366 \/ DSM 635 \/ J-10-fl\; ATCC 29364 \/ DSM 637 \/ Y-400-fl) Alpha\/beta hydrolase fold-containing protein Chloroflexus aurantiacus
chlad-b8g4g0 Chloroflexus aggregans (strain MD-66 \/ DSM 9485) Alpha\/beta hydrolase fold protein Chloroflexus aggregans
chlfr-PHAC Chlorogloeopsis fritschii phac Pha synthase Chlorogloeopsis fritschii
chrvi-phbc Chromatium vinosum (Allochromatium vinosum) PHA synthase subunit C (polyhydroxyalkanoic acid synthase) Chromatium vinosum
conwi-d3f2q0 Conexibacter woesei (strain DSM 14684 \/ JCM 11494 \/ NBRC 100937 \/ ID131577) Alpha\/beta hydrolase fold protein Conexibacter woesei
ectva-PHAC Ectothiorhodospira vacuolata pha synthase subunit phac Ectothiorhodospira vacuolata
ferpa-d3ryk7 Ferroglobus placidus (strain DSM 10642 \/ AEDII12DO) Alpha\/beta hydrolase fold protein Ferroglobus placidus
geoog-d2set8 Geodermatophilus obscurus (strain ATCC 25078 \/ DSM 43160 \/ JCM 3152 G-20) Alpha\/beta hydrolase fold protein Geodermatophilus obscurus
gluda-a9h1f4 Gluconacetobacter diazotrophicus (strain ATCC 49037 \/ DSM 5601 \/ PAl5) Putative poly-beta-hydroxybutyrate polymerase Gluconacetobacter diazotrophicus
gluda-b5zj06 Gluconacetobacter diazotrophicus (strain ATCC 49037 \/ DSM 5601 \/ PAl5) Alpha\/beta hydrolase fold Gluconacetobacter diazotrophicus
gorb4-d0l956 Gordonia bronchialis (strain ATCC 25592 \/ DSM 43247 \/ JCM 3198 \/ NCT 10667) (Rhodococcus bronchialis) AMP-dependent synthetase and ligase Gordonia bronchialis
grabc-q0bqk9 Granulibacter bethesdensis (strain ATCC BAA-1260 \/ CGDNIH1) Poly-beta-hydroxybutyrate polymerase Granulibacter bethesdensis
hahch-q2sl80 Hahella chejuensis (strain KCTC 2396) poly(3-hydroxyalkanoate) synthetase Hahella chejuensis
halhl-a1wz56 Halorhodospira halophila (strain DSM 244 \/ SL1) (Ectothiorhodospir halophila (strain DSM 244 \/ SL1)) Alpha\/beta hydrolase fold protein Halorhodospira halophila
halma-q5uym0 Haloarcula marismortui (Halobacterium marismortui) Haloarcula hispanica poly(3-hydroxyalkanoate) synthase subunit PhaC Haloarcula marismortui
halme-b3frm5 Halobacterium mediterranei (Haloferax mediterranei) Poly(3-hydroxyalkanoate) polymerase subunit PhaC Halobacterium mediterranei
magsa-q2w930 Magnetospirillum magneticum (strain AMB-1 \/ ATCC 700264) poly-beta-hydroxybutyrate polymerase Magnetospirillum magneticum
magsm-a0l6t8 Magnetococcus sp. (strain MC-1) Poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit Magnetococcus sp. MC-1
marav-a1u795 Marinobacter aquaeolei (strain ATCC 700491 \/ DSM 11845 \/ VT8 (Marinobacter hydrocarbonoclasticus (strain DSM 11845)) Alpha\/beta hydrolase fold protein Marinobacter hydrocarbonoclasticus VT8
metno-b8il10 Methylobacterium nodulans (strain ORS2060 \/ LMG 21967) PHA synthase subunit PhaC Methylobacterium nodulans
mets4-b0upq5 Methylobacterium sp. (strain 4-46) PHA synthase subunit PhaC Methylobacterium sp.
myca9-b1mb08 Mycobacterium abscessus (strain ATCC 19977 \/ DSM 44196) Possible long-chain acyl-CoA synthase Mycobacterium abscessus
myca9-b1mcm4 Mycobacterium abscessus (strain ATCC 19977 \/ DSM 44196) Putative hydrolase Mycobacterium abscessus
mycgi-a4t9x4 Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 \/ PYR-GCK)) AMP-dependent synthetase and ligase Mycobacterium gilvum
mycle-MLC1351.21C Mycobacterium leprae (and strain Br4923) hypothetical 109.2 kda protein Mycobacterium leprae
mycpa-q740g2 Mycobacterium paratuberculosis Mycobacterium avium (strain 104) hypothetical protein Mycobacterium paratuberculosis
mycs2-a0qys3 Mycobacterium smegmatis (strain ATCC 700084 \/ mc(2)155) Acyl-CoA synthase Mycobacterium smegmatis
mycss-q1b7r5 Mycobacterium sp. (strains MCS\; KMS\; JLS) AMP-dependent synthetase and ligase Mycobacterium sp.
myctu-RV1683 Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium marinum, Mycobacterium ulcerans,107.4 kda protein dehydrogenase (EC 6.2.1.-) long-chain Acyl-CoA synthetase RV1683 Mycobacterium tuberculosis
mycvp-a1ta99 Mycobacterium vanbaalenii (strain DSM 7251 \/ PYR-1) AMP-dependent synthetase and ligase Mycobacterium vanbaalenii
nocfa-q5yqw8 Nocardia farcinica putative hydrolase Nocardia farcinica
nocfa-q5yyb5 Nocardia farcinica putative Acyl-CoA synthetase Nocardia farcinica
nocsj-a1snv7 Nocardioides sp. (strain BAA-499 \/ JS614) Alpha\/beta hydrolase fold protein Nocardioides sp.
rhil3-q1m841 Rhizobium leguminosarum bv. viciae (strain 3841) putative polyhydroxyalkanoate synthase subunit c Rhizobium leguminosarum bv. viciae
rhocs-b6ir92 Rhodospirillum centenum (strain ATCC 51521 \/ SW) Polyhydroxyalkanoic acid synthase, putative Rhodospirillum centenum
rhoe4-c0zp80 Rhodococcus erythropolis (strain PR4 \/ NBRC 100887) Putative hydrolase Rhodococcus erythropolis
rhoer-c3jep8 Rhodococcus erythropolis SK121 Hydrolase, alpha\/beta fold family Rhodococcus erythropolis SK121
rhoer-c3jqs1 Rhodococcus erythropolis (strains SK121\; PR4 \/ NBRC 100887) Putative fatty-acid--CoA ligase and AMP-binding enzyme domain protein Rhodococcus erythropolis SK121
rhoob-c1asz4 Rhodococcus opacus (strain B4) Rhodococcus sp. (strain RHA1) Possible long-chain acyl-CoA synthase Putative fatty-acid--CoA ligase Rhodococcus opacus
rhoob-c1ba50 Rhodococcus opacus (strain B4) Putative hydrolase Rhodococcus opacus
rhort-q2rxr5 Rhodospirillum rubrum (strain ATCC 11170 \/ NCIB 8255) alpha\/beta hydrolase fold Rhodospirillum rubrum
rhosr-q0s8f4 Rhodococcus sp. (strain RHA1) Probable poly-beta-hydroxybutyrate polymerase Rhodococcus sp.
rubxd-q1atg5 Rubrobacter xylanophilus (strain DSM 9941 \/ NBRC 16129) Poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit Rubrobacter xylanophilus
strm5-b4smq6 Stenotrophomonas maltophilia (strains R551-3\; K279a)Stenotrophomonas sp. SKA14 Poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit Stenotrophomonas maltophilia
9clot-a0a1e8ew64 Clostridium acetireducens DSM 10703 Poly(3-hydroxyalkanoate) polymerase subunit Clostridium acetireducens DSM 10703
halwc-g0lkv6 Haloquadratum walsbyi (strain DSM 16854 \/ JCM 12705 \/ C23) Poly(3-hydroxyalkanoate) polymerase subunit Haloquadratum walsbyi C23
gloc7-PHAC Gloeothece citriformis (strain PCC 7424) (Cyanothece sp.) Poly(3-hydroxyalkanoate) polymerase subunit Gloeothece citriformis PCC 7424
synsp-PHAC Synechococcus sp. MA19 PHA synthase Synechococcus sp. MA19
synsp-PHBC Synechocystis sp.(strain PCC 6803 \/ Kazusa) poly(3-hydroxyalkanoate) synthase Synechocystis sp.
synww-q0axz5 Syntrophomonas wolfei subsp. wolfei (strain Goettingen) Poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit Syntrophomonas wolfei subsp. wolfei
thecd-d1abk7 Thermomonospora curvata (strain ATCC 19995 \/ DSM 43183 \/ JCM 3096 \/ NCIMB 10081) Alpha\/beta hydrolase fold protein Thermomonospora curvata
thivi-phbc Thiocystis violacea (polyhydroxyalkanoic acid synthase) Thiocystis violacea
xanax-PHBC Xanthomonas axonopodis (pv. citri) Xanthomonas campestris pv. vesicatoria (strain 85-10) poly (3-hydroxybutyric acid) synthase Xanthomonas axonopodis
xanca-PHBC Xanthomonas campestris (pv. campestris)( and strain 8004) poly (3-hydroxybutyric acid) synthase Xanthomonas campestris
xanor-q5h0p6 Xanthomonas oryzae pv. oryzae ( and strain MAFF 311018) poly (3-hydroxybutyric acid) synthase Xanthomonas oryzae pv. oryzae

Substrate Chemical Nomenclature Proteins
3-hydroxybutyrate bacce-PHAC    alceu-phbc    psefl-q6xh00
3HB-CoA S-[2-[3-[[(2R)-4-[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-2-hydroxy-3,3-dimethylbutanoyl]amino]propanoylamino]ethyl] 3-hydroxybutanethioate ralso-PHBC    alceu-phbc    chrvi-phbc    bacce-PHAC    psefl-q6xh00

References (3)

Title : Polyhydroxyalkanoate synthase (PhaC): The key enzyme for biopolyester synthesis - Neoh_2022_Curr.Res.Biotechnol_4_87
Author(s) : Neoh SZ , Chek MF , Tan HT , Linares-Pasten JA , Nandakumar A , Hakoshima T , Sudesh K
Ref : Current Research in Biotechnology , 4 :87 , 2022
Abstract :
PubMedSearch : Neoh_2022_Curr.Res.Biotechnol_4_87

Title : Polyester synthases: natural catalysts for plastics - Rehm_2003_Biochem.J_376_15
Author(s) : Rehm BH
Ref : Biochemical Journal , 376 :15 , 2003
Abstract :
PubMedSearch : Rehm_2003_Biochem.J_376_15
PubMedID: 12954080

Title : PhaC and PhaR are required for polyhydroxyalkanoic acid synthase activity in Bacillus megaterium - McCool_2001_J.Bacteriol_183_4235
Author(s) : McCool GJ , Cannon MC
Ref : Journal of Bacteriology , 183 :4235 , 2001
Abstract :
PubMedSearch : McCool_2001_J.Bacteriol_183_4235
PubMedID: 11418564
Gene_locus related to this paper: bacme-PHAC