Steryl_acetyl_hydrolase
Relationship
Comment
Sterol homeostasis in eukaryotic cells relies on the reciprocal interconversion of free sterols and steryl esters. In Saccharomyces cerevisiae (Baker's yeast) sterol acetylation requires the acetyltransferase Atf2, whereas deacetylation requires Say1, a membrane-anchored deacetylase with a putative active site in the ER lumen. Lack of Say1 results in the secretion of acetylated sterols into the culture medium, indicating that the substrate specificity of Say1 determines whether acetylated sterols are secreted from the cells or whether they are deacetylated and retained. In S. cerevisiae cells lacking Say1 or Atf2 are sensitive against the plant-derived allylbenzene eugenol and both Say1 and Atf2 affect pregnenolone toxicity, indicating that lipid acetylation acts as a detoxification pathway (old DUF2424)
Database
Interpro : IPR019436 Steryl_acetyl_hydrolase Say1-like
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF10340 Say1_Mug180
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Gene_locus | Name | Species |
|---|---|---|
| 9viru-a4l1y2 | Gryllus bimaculatus nudivirus Putative uncharacterized protein | Gryllus bimaculatus nudivirus |
| artoc-c5fwe0 | Arthroderma otae (strain CBS 113480) (Microsporum canis) Alpha\/beta hydrolase fold protein | Arthroderma otae |
| ashgo-q75a71 | Ashbya gossypii (strain ATCC 10895 \/ CBS 109.51 \/ FGSC 9923 \/ NRRY-1056) (Yeast) (Eremothecium gossypii) ADR047Wp | Ashbya gossypii |
| botfb-a6rm08 | Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) Predicted protein | Botryotinia fuckeliana |
| canal-q5a2u7 | Candida albicans (Yeast) Potential esterase\/lipase | Candida albicans |
| canal-q5ak09 | Candida albicans (Yeast) Potential esterase\/lipase | Candida albicans |
| canal-q5ak96 | Candida albicans (Yeast) Potential esterase\/lipase | Candida albicans |
| canal-q59l46 | Candida albicans (Yeast) Potential esterase\/lipase | Candida albicans |
| canal-q59nx4 | Candida albicans (Yeast) Potential esterase\/lipase | Candida albicans |
| canal-q59nx8 | Candida albicans (Yeast) Potential esterase\/lipase | Candida albicans |
| canaw-c4ykb1 | Candida albicans (strain WO-1) (Yeast) Putative uncharacterized protein | Candida albicans |
| canaw-c4yrn6 | Candida albicans (strain WO-1) (Yeast) Putative uncharacterized protein | Candida albicans |
| canaw-c4yrn9 | Candida albicans (strain WO-1) (Yeast) Putative uncharacterized protein | Candida albicans |
| canaw-c4yrr3 | Candida albicans (Yeast) Putative uncharacterized protein | Candida albicans |
| canaw-c4yrv3 | Candida albicans (strain WO-1) (Yeast) Putative uncharacterized protein | Candida albicans |
| canaw-c4ys26 | Candida albicans (strain WO-1) (Yeast) Putative uncharacterized protein | Candida albicans |
| candc-b9wa64 | Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Esterase\/lipase, putative | Candida dubliniensis |
| candc-b9wc93 | Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Hydrolase, putative | Candida dubliniensis |
| candc-b9whs3 | Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Esterase\/lipase, putative | Candida dubliniensis |
| candc-b9whs6 | Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Esterase\/lipase, putative | Candida dubliniensis |
| candc-b9whv2 | Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Esterase\/lipase, putative | Candida dubliniensis |
| candc-b9wi60 | Candida dubliniensis (strain CD36 \/ CBS 7987 \/ NCPF 3949 \/ NRRY-17841) (Yeast) Esterase\/lipase, putative | Candida dubliniensis |
| canga-q6ftv9 | Candida glabrata (Yeast) (Torulopsis glabrata) Similar to uniprot|P53324 Saccharomyces cerevisiae YGR263c | Candida glabrata |
| cantt-c5m5e8 | Candida tropicalis (strain ATCC MYA-3404 \/ T1) (Yeast) Putative uncharacterized protein | Candida tropicalis |
| cantt-c5mh80 | Candida tropicalis (strain ATCC MYA-3404 \/ T1) (Yeast) Putative uncharacterized protein | Candida tropicalis |
| cantt-c5mh89 | Candida tropicalis (strain ATCC MYA-3404 \/ T1) (Yeast) Putative uncharacterized protein | Candida tropicalis |
| cantt-c5mhh0 | Candida tropicalis (strain ATCC MYA-3404 \/ T1) (Yeast) Putative uncharacterized protein | Candida tropicalis |
| clal4-c4xy03 | Clavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae) Putative uncharacterized protein | Clavispora lusitaniae |
| clal4-c4yba6 | Clavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae) Putative uncharacterized protein | Clavispora lusitaniae |
| clal4-c4yba7 | Clavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae) Putative uncharacterized protein | Clavispora lusitaniae |
| copc7-a8nkc7 | Coprinopsis cinerea (strain Okayama-7 \/ 130 \/ FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata) Putative uncharacterized protein | Coprinopsis cinerea |
| debha-b5rue8 | Debaryomyces hansenii (Yeast) (Torulaspora hansenii) DEHA2F11968p | Debaryomyces hansenii |
| debha-b5rue9 | Debaryomyces hansenii (Yeast) (Torulaspora hansenii) DEHA2F11990p | Debaryomyces hansenii |
| klula-q6cm49 | Kluyveromyces lactis (Yeast) (Candida sphaerica) KLLA0E23013p | Kluyveromyces lactis |
| lactc-c5dif7 | Lachancea thermotolerans (strain CBS 6340) (Yeast) (Kluyveromyces thermotolerans) KLTH0E12166p | Lachancea thermotolerans |
| lodel-a5duy7 | Lodderomyces elongisporus (Yeast) (Saccharomyces elongisporus) Putative uncharacterized protein | Lodderomyces elongisporus |
| lodel-a5e2s1 | Lodderomyces elongisporus (Yeast) (Saccharomyces elongisporus) Putative uncharacterized protein | Lodderomyces elongisporus |
| lodel-a5e2t8 | Lodderomyces elongisporus (Yeast) (Saccharomyces elongisporus) Putative uncharacterized protein | Lodderomyces elongisporus |
| lodel-a5e2v2 | Lodderomyces elongisporus (Yeast) (Saccharomyces elongisporus) Putative uncharacterized protein | Lodderomyces elongisporus |
| neucr-a7uwy7 | Neurospora crassa (strain ATCC 24698 \/ 74-OR23-1A \/ CBS 708.71 \/ DSM 1257 \/ FGSC 987) hypothetical protein | Neurospora crassa |
| penmq-b6qb64 | Penicillium marneffei (strain ATCC 18224 \/ CBS 334.59 \/ QM 7333) Putative uncharacterized protein | Penicillium marneffei |
| picgu-a5dc73 | Pichia guilliermondii (Yeast) (Candida guilliermondii) Putative uncharacterized protein | Pichia guilliermondii |
| picgu-a5dc74 | Pichia guilliermondii (Yeast) (Candida guilliermondii) Putative uncharacterized protein | Pichia guilliermondii |
| picgu-a5dc75 | Pichia guilliermondii (Yeast) (Candida guilliermondii) Putative uncharacterized protein | Pichia guilliermondii |
| picgu-a5dnr3 | Pichia guilliermondii (Yeast) (Candida guilliermondii) Putative uncharacterized protein | Pichia guilliermondii |
| picgu-a5drl3 | Pichia guilliermondii (Yeast) (Candida guilliermondii) Putative uncharacterized protein | Pichia guilliermondii |
| picst-a3ln06 | Pichia stipitis (Yeast) Arylacetamide deacetylase | Pichia stipitis |
| picst-a3lvi5 | Pichia stipitis (Yeast) Hypothetical membrane protein | Pichia stipitis |
| picst-a3lvu9 | Pichia stipitis (Yeast) Predicted protein | Pichia stipitis |
| podan-b2avm3 | Podospora anserina Predicted CDS Pa_7_2450 | Podospora anserina |
| pyrtr-b2w5w8 | Pyrenophora tritici-repentis (strain Pt-1C-BFP) (Wheat tan spot fungus) (Drechslera tritici-repentis) Alpha\/beta hydrolase fold domain containing protein | Pyrenophora tritici-repentis |
| pyrtr-b2wia5 | Pyrenophora tritici-repentis (strain Pt-1C-BFP) (Wheat tan spot fungus) (Drechslera tritici-repentis) Putative uncharacterized protein | Pyrenophora tritici-repentis |
| pyrtt-e3rgg7 | Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus)(Drechslera teres f. teres) Putative uncharacterized protein | Pyrenophora teres f. teres |
| pyrtt-e3sa06 | Pyrenophora teres f. teres (strain 0-1) (Barley net blotch fungus) (Drechslera teres f. teres) Putative uncharacterized protein | Pyrenophora teres f. teres |
| schpo-SPBPB2B2.02 | Schizosaccharomyces pombe (Fission yeast) and (strain ATCC 38366 \/ 972) Putative steryl acetyl hydrolase mug180 Meiotically up-regulated gene 180 protein | Schizosaccharomyces pombe |
| scls1-a7el43 | Sclerotinia sclerotiorum (strain ATCC 18683 \/ 1980 \/ Ss-1) (White mold) (Whetzelinia sclerotiorum) Predicted protein | Sclerotinia sclerotiorum |
| talsn-b8m0h7 | Talaromyces stipitatus (strain ATCC 10500 \/ CBS 375.48 \/ QM 6759 \/ NRRL 1006) (Penicillium stipitatum) Putative uncharacterized protein | Talaromyces stipitatus |
| tubmm-d5glm4 | Tuber melanosporum (strain Mel28) (Perigord black truffle) GSTUM_00010271001 | Tuber melanosporum |
| vanpo-a7tnv7 | Vanderwaltozyma polyspora (strain ATCC 22028 \/ DSM 70294) (Kluyveromyces polysporus) Putative uncharacterized protein | Vanderwaltozyma polyspora |
| yarli-q6c493 | Yarrowia lipolytica (Candida lipolytica) similar to wi|ncu07890.1 neurospora crassa ncu07890.1 | Yarrowia lipolytica |
| yeast-SAY1 | Saccharomyces cerevisiae (Baker's yeast) hypothetical 48.5da prot YG5J chromosome VII | Saccharomyces cerevisiae |
| zygrc-c5dvl2 | Zygosaccharomyces rouxii (strain ATCC 2623 \/ CBS 732 \/ IFO 1130 \/ NBR1623 \/ NCYC 568) (Candida mogii) ZYRO0D07590p | Zygosaccharomyces rouxii |
| macph-k2ryd3 | Macrophomina phaseolina (strain MS6) (Charcoal rot fungus) Carboxylesterase type B | Macrophomina phaseolina |
| fusox-w9ibd7 | Fusarium oxysporum Uncharacterized protein | Fusarium oxysporum FOSC 3-a |
| Gene_locus_frgt | Name | Species |
|---|---|---|
| monpe-e2l6w1 | Moniliophthora perniciosa (strain FA553 \/ isolate CP02)(Witches'-broom disease fungus) (Marasmius perniciosus) Putative uncharacterized protein | Moniliophthora perniciosa |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| Cholesterol-acetate | [(3S)-10,13-dimethyl-17-(6-methylheptan-2-yl)-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-yl] acetate |
References (1)
| Title : An acetylation\/deacetylation cycle controls the export of sterols and steroids from S. cerevisiae - Tiwari_2007_EMBO.J_26_5109 |
| Author(s) : Tiwari R , Koffel R , Schneiter R |
| Ref : EMBO Journal , 26 :5109 , 2007 |
| Abstract : |
| PubMedSearch : Tiwari_2007_EMBO.J_26_5109 |
| PubMedID: 18034159 |
| Gene_locus related to this paper: yeast-SAY1 |