AHL-acylase
Relationship
Comment
Bacterial AHL-acylases (acyl homoserine lactonase) (AiiO, AidH) from genus Ochrobactrum and related bacteria are different from previously described AHL-acylases such as PvdQ, QuiP (P.aeriginosa) HacA HacB (P.syringea which belong to Ntn-hydrolase superfamily Czajkowski et al. 2009, Czajkowski et al. 2011) and different from AHL-lactonase of B. thuringensis and A. tumefasciens (which are metallo-beta lactamases). AHL-acylase inactivates N-acyl homoserine lactone (AHL) quorum sensing signal molecules (lactonases are also inactivating enzymes). AHL-acylases could be useful for the control of development of infections caused by pathogenic bacteria and their persistence in respective hosts. The three fungi sequences are genes without real introns (horizontal transfer?). There are other N-acyl homoserine lactonase (AiiA) not alpha/beta hydroalses but Metallo-B-lactamases
Database
Interpro : No interpro
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF12697 Abhydrolase_6
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 4G5X | Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum | 9rhiz-d2j2t6 |
| 4G8B | Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum S102G mutant complexed with N-hexanoyl homoserine lactone | 9rhiz-d2j2t6 |
| 4G8C | Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum E219G mutant complexed with N-hexanoyl homoserine | 9rhiz-d2j2t6 |
| 4G8D | Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum S102G mutant | 9rhiz-d2j2t6 |
| 4G9E | Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum complexed with N-butanoyl homoserine | 9rhiz-d2j2t6 |
| 4G9G | Crystal structure of N-acyl homoserine lactonase AidH from Ochrobactrum E219G mutant | 9rhiz-d2j2t6 |
| Gene_locus | Name | Species |
|---|---|---|
| 9hyph-a0a256fxx7 | Brucella thiophenivorans Alpha\/beta hydrolase | Brucella thiophenivorans |
| 9hyph-a0a2r3sty9 | Pseudochrobactrum asaccharolyticum esterase pae07 | Pseudochrobactrum asaccharolyticum |
| 9brad-a3wre8 | Nitrobacter sp. Nb-311A Alpha\/beta hydrolase fold protein | Nitrobacter sp. Nb-311A |
| 9rhiz-c4wkm9 | Ochrobactrum intermedium LMG 3301 Abhydrolase domain-containing protein 8 | Ochrobactrum intermedium LMG 3301 |
| 9rhiz-c8sqp9 | Mesorhizobium opportunistum WSM2075 Alpha\/beta hydrolase fold protein | Mesorhizobium opportunistum WSM2075 |
| 9rhiz-d2j2t6 |  Ochrobactrum sp. T63 AidH acyl homoserine lactonase |
Ochrobactrum sp. T63 |
| 9rhiz-d8x182 | Ochrobactrum sp. A44 Alpha\/beta hydrolase fold protein | Ochrobactrum sp. A44 |
| agrvs-b9k312 | Agrobacterium vitis (strain S4 \/ ATCC BAA-846) (Rhizobium vitis (strain S4)) Alpha\/beta hydrolase protein | Agrobacterium vitis |
| mago7-a4rdm3 | Magnaporthe oryzae (strain 70-15 \/ FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) Alpha\/beta hydrolase | Magnaporthe oryzae |
| nithx-q1qq48 | Nitrobacter hamburgensis (strain X14 \/ DSM 10229) alpha\/beta hydrolase fold | Nitrobacter hamburgensis |
| nitwn-q3svd2 | Nitrobacter winogradskyi (strain Nb-255 \/ ATCC 25391) alpha\/beta hydrolase fold | Nitrobacter winogradskyi |
| ocha4-a6wx49 | Ochrobactrum anthropi\; Ochrobactrum sp. AiiO-AIO6 N-acyl homoserine lactonase | Ochrobactrum anthropi |
| olico-b6jdg5 | Oligotropha carboxidovorans (strain ATCC 49405 \/ DSM 1227 \/ OM5) Alpha\/beta hydrolase fold protein | Oligotropha carboxidovorans |
| parl1-a7hv92 | Parvibaculum lavamentivorans (strain DS-1 \/ DSM 13023 \/ NCIMB 13966) Alpha\/beta hydrolase fold | Parvibaculum lavamentivorans |
| pedhd-c6y3z2 | Pedobacter heparinus (strain ATCC 13125 \/ DSM 2366 \/ NCIB 9290) Alpha\/beta hydrolase fold protein | Pedobacter heparinus |
| penmq-b6qcs8 | Penicillium marneffei (strain ATCC 18224 \/ CBS 334.59 \/ QM 7333) Valacyclovir hydrolase, putative | Penicillium marneffei |
| phano-q0upq4 | Phaeosphaeria nodorum (Septoria nodorum) putative uncharacterized protein | Phaeosphaeria nodorum |
| pholu-PLU2437 | Photorhabdus luminescens (subsp. laumondii) Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 \/ 3105-77) (Xenorhabdus luminescens (strain 2)) hydrolase | Photorhabdus luminescens |
| rhoe4-c0ztk5 | Rhodococcus erythropolis (strain PR4 \/ NBRC 100887) Putative hydrolase | Rhodococcus erythropolis |
| rhoer-c3jn30 | Rhodococcus erythropolis SK121 Hydrolase, alpha\/beta fold family | Rhodococcus erythropolis SK121 |
| rhop2-q2j0u2 | Rhodopseudomonas palustris (strain HaA2) alpha\/beta hydrolase | Rhodopseudomonas palustris |
| sacd2-q21ez0 | Saccharophagus degradans (strain 2-40 \/ ATCC 43961 \/ DSM 17024) Hydrolases or acyltransferases (Alpha\/beta hydrolase superfamily)-like | Saccharophagus degradans |
| talsn-b8ly90 | Talaromyces stipitatus (strain ATCC 10500 \/ CBS 375.48 \/ QM 6759 \/ NRRL 1006) (Penicillium stipitatum) Alpha\/beta hydrolase, putative | Talaromyces stipitatus |
| xenbs-d3v3f7 | Xenorhabdus bovienii (strains SS-2004\; SS-2004) Putative Epoxide hydrolase | Xenorhabdus bovienii |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| N-Ac-DL-MetOMe | methyl 2-acetamido-4-methylsulfanylbutanoate | 9hyph-a0a256fxx7 |
| (R,S)-DMPM | methyl 2-(2,6-dimethylanilino)propanoate | burce-lipaa 9hyph-a0a2r3sty9 |
| C6-HSL | N-[(3S)-2-oxooxolan-3-yl]hexanamide | 9rhiz-d2j2t6 9bact-6EB3 |
| 3-oxo-C12-HSL | 3-oxo-N-[(3S)-2-oxooxolan-3-yl]dodecanamide |
| Inhibitor | Chemical Nomenclature | Proteins |
|---|---|---|
| N-Hexanoyl-L-Homoserine | (2S)-2-(hexanoylamino)-4-hydroxybutanoic acid | 9rhiz-d2j2t6 |
| N-butanoyl-L-homoserine | (2S)-2-(butanoylamino)-4-hydroxybutanoic acid | 9rhiz-d2j2t6 |
References (4)
| Title : High-resolution structures of AidH complexes provide insights into a novel catalytic mechanism for N-acyl homoserine lactonase - Gao_2013_Acta.Crystallogr.D.Biol.Crystallogr_69_82 |
| Author(s) : Gao A , Mei GY , Liu S , Wang P , Tang Q , Liu YP , Wen H , An XM , Zhang LQ , Yan XX , Liang DC |
| Ref : Acta Crystallographica D Biol Crystallogr , 69 :82 , 2013 |
| Abstract : |
| PubMedSearch : Gao_2013_Acta.Crystallogr.D.Biol.Crystallogr_69_82 |
| PubMedID: 23275166 |
| Gene_locus related to this paper: 9rhiz-d2j2t6 |
| Title : Inactivation of AHLs by Ochrobactrum sp. A44 depends on the activity of a novel class of AHL acylase - Czajkowski_2011_Environ.Microbiol.Rep_3_59 |
| Author(s) : Czajkowski R , Krzyzanowska D , Karczewska J , Atkinson S , Przysowa J , Lojkowska E , Williams P , Jafra S |
| Ref : Environ Microbiol Rep , 3 :59 , 2011 |
| Abstract : |
| PubMedSearch : Czajkowski_2011_Environ.Microbiol.Rep_3_59 |
| PubMedID: 23761232 |
| Gene_locus related to this paper: 9rhiz-d8x182 |
| Title : AidH, an alpha\/beta-hydrolase fold family member from an Ochrobactrum sp. strain, is a novel N-acylhomoserine lactonase - Mei_2010_Appl.Environ.Microbiol_76_4933 |
| Author(s) : Mei GY , Yan XX , Turak A , Luo ZQ , Zhang LQ |
| Ref : Applied Environmental Microbiology , 76 :4933 , 2010 |
| Abstract : |
| PubMedSearch : Mei_2010_Appl.Environ.Microbiol_76_4933 |
| PubMedID: 20525860 |
| Gene_locus related to this paper: 9rhiz-d2j2t6 |
| Title : Quenching of acyl-homoserine lactone-dependent quorum sensing by enzymatic disruption of signal molecules. - Czajkowski_2009_Acta.Biochim.Pol_56_1 |
| Author(s) : Czajkowski R , Jafra S |
| Ref : Acta Biochim Pol , 56 :1 , 2009 |
| Abstract : |
| PubMedSearch : Czajkowski_2009_Acta.Biochim.Pol_56_1 |
| PubMedID: 19287806 |