Gene_locus Report for: alceu-lipas Alcaligenes eutrophus, Cupriavidus necator (Ralstonia eutropha) gene for lipase
Comment
Other strains: Cupriavidus necator (Alcaligenes eutrophus) (Ralstonia eutropha); (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) Species new name Ralstonia eutropha sequence also named ORF8 Relationship
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) cellular organisms: N E > Bacteria: N E > Proteobacteria: N E > Betaproteobacteria: N E > Burkholderiales: N E > Burkholderiaceae: N E > Cupriavidus: N E > Cupriavidus necator: N E 5_AlphaBeta_hydrolase :
cupnh-q0jzs8 Ralstonia eutropha Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) putative uncharacterized protein h16_b1964 ,
cupnh-q0k226 Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) hydrolase of the alpha/beta superfamily .
6_AlphaBeta_hydrolase :
cuppj-q46mp5 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cuppj-q46pw1 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cuppj-q46rf2 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cuppj-q46t91 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) hydrolase, alpha/beta fold family ,
cuppj-q46va8 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cuppj-q471f8 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cuppj-q472i5 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cuppj-q472p0 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cuppj-q473f7 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cuppj-q473g8 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold:thioesterase ,
cupnh-q0k2b3 Ralstonia eutropha (Cupriavidus necator strain ATCC 17699 / H16 / DSM 428 / Stanier 337) AlphaBeta_hydrolase ,
cupnh-q0k9g4 Ralstonia eutropha Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) predicted hydrolase or acyltransferase (EC 3.-.-.-) ,
cupnh-q0k189 Ralstonia eutropha (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) putative uncharacterized protein h16_b1447 ,
cupnh-q0k399 Ralstonia eutropha (Cupriavidus necator strain ATCC 17699 / H16 / DSM 428 / Stanier 337) AlphaBeta_hydrolase superfamily ,
cupnh-q0kbr4 Ralstonia eutropha (Cupriavidus necator strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (EC 3.1.1.-) ,
cupnh-q0kbs3 Ralstonia eutropha (Cupriavidus necator strain ATCC 17699 / H16 / DSM 428 / Stanier 337) predicted hydrolase or acyltransferase (EC 3.-.-.-) ,
cupnh-q0kcd2 Ralstonia eutropha (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337 )) ,
cupnh-q0kci6 Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337 predicted hydrolase or acyltransferase (EC 3.-.-.-) .
A85-EsteraseD-FGH :
cuppj-q46u29 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) carboxylesterase (EC 3.1.1.1) .
abh_upf0017 :
cuppj-q475k8 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold .
abh_upf00227 :
cuppj-q46xb2 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) hypothetical protein .
AcoC_BiotinLipoyl-ABH :
cuppj-q46pn7 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold:biotin/lipoyl attachment ,
cupnh-acoc Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337 ) (fast-migrating protein) (fmp) dihydrolipoamide acetyltransferase DHLTA .
Atu1826-like :
cupnh-q0k038 Ralstonia eutropha Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) putative hydrolase alpha/beta fold .
CarbLipBact_2 :
cuppj-q46s74 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold .
Carbon-carbon_bond_hydrolase :
cuppj-q46p99 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cuppj-q471q3 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cupnh-q0k3m7 Ralstonia eutropha (Cupriavidus necator strain ATCC 17699 / H16 / DSM 428 / Stanier 337) 2,6-dioxo-6-phenylhexa-3-enoate hydrolase (EC 3.7.1.8) ,
cupnh-q0k199 Ralstonia eutropha (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) (3.-.-.-) .
Carboxymethylbutenolide_lactonase :
alceu-catD1 Ralstonia eutropha; Ralstonia sp.; Cupriavidus necator; Cupriavidus sp., 3-oxoadipate enol-lactone hydrolase (catD1) gene (EC 3.1.1.24) ,
alceu-catD2 Ralstonia eutropha; Cupriavidus necator; Ralstonia sp.; Cupriavidus sp., 3-oxoadipate enol-lactone hydrolase (catD2) gene (EC 3.1.1.24) ,
cuppj-q46r64 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) carboxymuconolactone decarboxylase ,
cuppj-q470x6 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase ,
cuppj-q477d6 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cupnh-q0jyv4 Ralstonia eutropha (Cupriavidus necator); Ralstonia sp. Transcriptional regulator AraC familydecarboxylase (EC 4.1.1.44) ,
cupnh-q0kfc2 Ralstonia eutropha (Cupriavidus necator strain ATCC 17699 / H16 / DSM 428 / Stanier 337)AlphaBeta_hydrolase superfamily) .
Carboxypeptidase_S10 :
cuppj-q470r0 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) putative carboxypeptidase .
Cocaine_esterase :
cuppj-q470m2 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) peptidase s15 .
Dienelactone_hydrolase :
alceu-CBNC Alcaligenes eutrophus Cupriavidus necator (Ralstonia eutropha), Pseudomonas chlororaphis (P. fluorescens) Achromobacter xylosoxidans, Comamonas acidovorans, P. aeruginosa, Bordetella petrii Delftia acidovorans (Ps. acidovorans) (Comamonas acidovorans), Bordetella sp., Ps. nitroreducens, Alcaligenes xylosoxydans xylosoxydans (Achromobacter xylosoxidans) dienelactone hydrolase (EC 3.1.1.45) tfdE tcbE ,
alceu-tfe1 Alcaligenes eutrophus, Cupriavidus necator (Ralstonia eutropha) Dienelactonehydrolase (dlh I) ,
alceu-tfe2 Alcaligenes eutrophus, Cupriavidus necator (Ralstonia eutropha) Dienelactone hydrolase (dlh II) ,
cuppj-q46me8 Cupriavidus pinatubonensis (Alcaligenes eutrophus) (Ralstonia eutropha) carboxymethylenebutenolidase (EC 3.1.1.45) ,
cuppj-q46mk8 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) carboxymethylenebutenolidase (EC 3.1.1.45) ,
cuppj-q46p20 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) carboxymethylenebutenolidase (EC 3.1.1.45) ,
cuppj-q46pl2 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) carboxymethylenebutenolidase (EC 3.1.1.45) ,
cuppj-q46we3 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) carboxymethylenebutenolidase (EC 3.1.1.45) ,
cuppj-q474c9 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) twin-arginine translocation pathway signal (EC 3.1.1.45) ,
cuppj-q477h9 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) dienelactone hydrolase .
Duf_1100-S :
cuppj-q46mv6 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) acetyl xylan esterase .
Epoxide_hydrolase :
cuppj-q46s00 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) twin-arginine translocation pathway signal ,
cuppj-q471z7 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cuppj-q472r0 Ralstonia eutropha (Cupriavidus necator, Alcaligenes eutrophus) lysophospholipase (EC 3.1.1.5) alpha/beta hydrolase fold:thioesterase ,
cupnh-q0k7y4 Ralstonia eutropha (Cupriavidus necator strain ATCC 17699 / H16 / DSM 428 / Stanier 337) Putative peptidase, S33 family .
Esterase_phb :
cuppj-q46qx3 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) esterase, phb depolymerase ,
cuppj-q46v50 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) esterase, phb depolymerase .
Haloacetate_dehalogenase :
cuppj-q476y6 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold .
Haloperoxidase :
cuppj-q46qm9 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cupnh-q0jzu9 Ralstonia eutropha (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) putative non-heme chloroperoxidase (EC 1.11.1.10) ,
cupnh-q0k1a8 Ralstonia eutropha (Cupriavidus necator ((strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) non-heme haloperoxidase (EC 1.11.1.-) ,
cupnh-q0k1c5 Ralstonia eutropha (Cupriavidus necator ((strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) non-heme chloroperoxidase (EC 1.11.1.10) ,
cupnh-q0k9l1 Ralstonia eutropha (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) non-heme haloperoxidase .
Homoserine_transacetylase :
cuppj-metx Ralstonia eutropha, Alcaligenes eutrophus, Cupriavidus necator, Cupriavidus taiwanensis, Ralstonia taiwanensis, homoserine o-acetyltransferase (EC 2.3.1.31) .
Hormone-sensitive_lipase_like :
cuppj-q46mt4 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) esterase/lipase/thioesterase ,
cuppj-q46na0 Cupriavidus pinatubonensis (Alcaligenes eutrophus) (Ralstonia eutropha), Geobacter sp., Dickeya dadantii, esterase/lipase/thioesterase ,
cuppj-q46px7 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) esterase/lipase/thioesterase ,
cuppj-q46t45 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) esterase/lipase/thioesterase ,
cuppj-q46vy3 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) lipolytic enzyme ,
cuppj-q471n0 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) lipolytic enzyme ,
cuppj-q471z4 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) lipolytic enzyme ,
cupnh-q0k3j4 Cupriavidus necator (Ralstonia eutropha) Esterase/lipase .
Hydrolase_RBBP9_YdeN :
cuppj-q46zd6 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) hypothetical protein ,
cuppj-q46zu8 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) hypothetical protein ,
cuppj-q471g3 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) hypothetical protein .
LYsophospholipase_carboxylesterase :
cuppj-q46s40 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) phospholipase/carboxylesterase ,
cuppj-q46t84 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) carboxylesterase (EC 3.1.1.1) ,
cuppj-q46tk7 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) phospholipase/carboxylesterase .
Monoglyceridelipase_lysophospholip :
cuppj-q46u16 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cuppj-q46vv2 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold .
NFM-deformylase :
cuppj-q46un9 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold ,
cupnh-q0k320 Ralstonia eutropha (Cupriavidus necator) (strain ATCC 17699 / H16 / DSM 428 / Stanier 337), Cupriavidus sp., Ralstonia sp hydrolase ,
cupnh-q0kd51 Ralstonia eutropha (cupriavidus necator), Ralstonia sp., Cupriavidus sp. predicted hydrolase .
PGAP1 :
cuppj-q46qh0 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) hypothetical protein ,
cuppj-q473w2 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) putative lipase transmembrane protein .
PHA_depolymerase_arom :
cuppj-q46zd3 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) poly(3-hydroxyalkanoate) depolymerase .
PHA_synth_I :
alceu-phbc ,
cuppj-q472g6 Cupriavidus pinatubonensis (Alcaligenes eutrophus) (Ralstonia eutropha) poly(r)-hydroxyalkanoic acid synthase, class i .
PHA_synth_II :
cuppj-q46zd1 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) terminal .
PHB_depolymerase_PhaZ :
cuppj-q46s33 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) polyhydroxyalkanoate depolymerase ,
cuppj-q473k5 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) polyhydroxyalkanoate depolymerase .
Proline_iminopeptidase :
cuppj-q46vi4 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) peptidase s33, proline iminopeptidase 1 (EC 3.4.11.5) .
Prolyl_oligopeptidase_S9 :
cuppj-q477a5 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) conserved hypothetical signal peptide protein .
Tannase :
cuppj-q46nh7 Ralstonia eutropha (Alcaligenes eutrophus) tannase and feruloyl esterase ,
cuppj-q46rl3 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) feruloyl esterase (EC 3.1.1.73) ,
cuppj-q471q7 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) tannase and feruloyl esterase .
Xaa-Pro-like_dom :
cuppj-q46na4 Cupriavidus pinatubonensis (Alcaligenes eutrophus) (Ralstonia eutropha); Cupriavidus basilensis; Variovorax sp. hypothetical protein ,
cuppj-q46qt0 Cupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) alpha/beta hydrolase fold Warning: This entry is a compilation of different species or line or strain with more than 90% amino acide identity. You can
retrieve all strain data
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.) Alcaligenes eutrophus:
N ,
E .
Cupriavidus necator:
N ,
E .
Ralstonia eutropha H16:
N ,
E .
Cupriavidus necator N-1:
N ,
E .
Molecular evidence
Database
No mutation No structure No kinetic No Substrate No inhibitor
Sequence
Graphical view for this peptide sequence: alceu-lipas Colored MSA for Hormone-sensitive_lipase_like (raw)
MSRCGRRAELLHAAGTGSGSASFPMSAAPSSSPDAPATPGLPSSPLDPQV
AALLELIARAKRPPIHAMEPEDAKIAYEKSAPILDINPPPVYMAEDLLAP
ARDGHAIPLRLYTPREASWTEPLPLLVYFHGGGFTVGSVDSHDPLCRLLC
GQADCMVLSVDYRLGPQWRFPTAANDAFDVLHWVFAEAGRLGADPARIAV
GGDSAGGTLAAACAVEARNAGLAPVLQLLIYPGTCARQDTPSHRALADGY
LLTADMIRWFFAQYLDQEASRDDWRFAPLDGGGAGAEVRGTCPAWIAVAG
YDPLHDEGVAYAEKLRAAGVAATLADYPGMIHDFFKLGRFVPAVAQAHAE
AVAALRAAFGTPHN
Legend
This sequence has been compared to family alignement (MSA) red => minority aminoacid blue => majority aminoacid color intensity => conservation rate title => sequence position(MSA position)aminoacid rate Catalytic site Catalytic site in the MSA
M S R C G R R A E L L H A A G T G S G S A S F P M S A A P S S S P D A P A T P G L P S S P L D P Q V A A L L E L I A R A K R P P I H A M E P E D A K I A Y E K S A P I L D I N P P P V Y M A E D L L A P A R D G H A I P L R L Y T P R E A S W T E P L P L L V Y F H G G G F T V G S V D S H D P L C R L L C G Q A D C M V L S V D Y R L G P Q W R F P T A A N D A F D V L H W V F A E A G R L G A D P A R I A V G G D S A G G T L A A A C A V E A R N A G L A P V L Q L L I Y P G T C A R Q D T P S H R A L A D G Y L L T A D M I R W F F A Q Y L D Q E A S R D D W R F A P L D G G G A G A E V R G T C P A W I A V A G Y D P L H D E G V A Y A E K L R A A G V A A T L A D Y P G M I H D F F K L G R F V P A V A Q A H A E A V A A L R A A F G T P H N
References
Title: Genome sequence of the bioplastic-producing Knallgas bacterium Ralstonia eutropha H16Pohlmann A , Fricke WF , Reinecke F , Kusian B , Liesegang H , Cramm R , Eitinger T , Ewering C , Potter M and Bowien B <6 more author(s)> Pohlmann A , Fricke WF , Reinecke F , Kusian B , Liesegang H , Cramm R , Eitinger T , Ewering C , Potter M , Schwartz E , Strittmatter A , Voss I , Gottschalk G , Steinbuchel A , Friedrich B , Bowien B (- 6) Ref: Nat Biotechnol, 24 :1257, 2006 : PubMed Abstract ESTHER: Pohlmann_2006_Nat.Biotechnol_24_1257 PubMedSearch: Pohlmann 2006 Nat.Biotechnol 24 1257 PubMedID: 16964242 Gene_locus related to this paper: alceu-catD1 ,
alceu-catD2 ,
alceu-lipas ,
alceu-q4w8c9 ,
cupne-q7wt49 ,
cupnh-q0jy77 ,
cupnh-q0k0b3 ,
cupnh-q0k0b5 ,
cupnh-q0k0g2 ,
cupnh-q0k0l5 ,
cupnh-q0k0q7 ,
cupnh-q0k2d0 ,
cupnh-q0k4q3 ,
cupnh-q0k4r4 ,
cupnh-q0k9j6 ,
cupnh-q0ka76 ,
cupnh-q0kab1 ,
cupnh-q0kat1 ,
cupnh-q0kcu9 ,
cupnh-q0kd98 ,
cupnh-q0kdv2 ,
cupnh-q0ke65 ,
cuppj-metx ,
cuppj-q472r0 ,
cuptr-b2agb4 ,
cuptr-b3r9z0 ,
cuptr-b3r543 ,
cupnh-acoc ,
cupnh-q0jyv4 ,
cupnh-q0jzs8 ,
cupnh-q0jzu9 ,
cupnh-q0k1a8 ,
cupnh-q0k1c5 ,
cupnh-q0k2b3 ,
cupnh-q0k3m7 ,
cupnh-q0k7y4 ,
cupnh-q0k9g4 ,
cupnh-q0k9l1 ,
cupnh-q0k038 ,
cupnh-q0k189 ,
cupnh-q0k199 ,
cupnh-q0k226 ,
cupnh-q0k320 ,
cupnh-q0k399 ,
cupnh-q0kbr4 ,
cupnh-q0kbs3 ,
cupnh-q0kcd2 ,
cupnh-q0kci6 ,
cupnh-q0kd51 ,
cupnh-q0kfc2 ,
ralpi-u3qr80 ,
cupnn-g0ewh7 ,
cupnh-hboh ,
cupnh-q0kdw6 Abstract
The H(2)-oxidizing lithoautotrophic bacterium Ralstonia eutropha H16 is a metabolically versatile organism capable of subsisting, in the absence of organic growth substrates, on H(2) and CO(2) as its sole sources of energy and carbon. R. eutropha H16 first attracted biotechnological interest nearly 50 years ago with the realization that the organism's ability to produce and store large amounts of poly[R-(-)-3-hydroxybutyrate] and other polyesters could be harnessed to make biodegradable plastics. Here we report the complete genome sequence of the two chromosomes of R. eutropha H16. Together, chromosome 1 (4,052,032 base pairs (bp)) and chromosome 2 (2,912,490 bp) encode 6,116 putative genes. Analysis of the genome sequence offers the genetic basis for exploiting the biotechnological potential of this organism and provides insights into its remarkable metabolic versatility.
Title: Metabolic pathway for biosynthesis of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from 4-hydroxybutyrate by Alcaligenes eutrophusValentin HE , Zwingmann G , Schonebaum A , Steinbuchel A Ref: European Journal of Biochemistry, 227 :43, 1995 : PubMed Abstract ESTHER: Valentin_1995_Eur.J.Biochem_227_43 PubMedSearch: Valentin 1995 Eur.J.Biochem 227 43 PubMedID: 7851418 Gene_locus related to this paper: alceu-lipas Abstract
Various aerobic Gram-negative bacteria have been examined for their ability to use 4-hydroxybutyrate and 1,4-butanediol as carbon source for growth. Alcaligenes eutrophus strains H16, HF39, PHB-4 and Pseudomonas denitrificans 'Morris' were not able to grow with 1,4-butanediol or 4-hydroxybutyrate. From A. eutrophus HF39 spontaneous primary mutants (e.g. SK4040) were isolated which grew on 4-hydroxybutyrate with doubling times of approximately 3 h. Tn5::mob mutagenesis of mutant SK4040 led to the isolation of two phenotypically different classes of secondary mutants which were affected in the utilization of 4-hydroxybutyrate. Mutants exhibiting the phenotype 4-hydroxybutyrate-negative did not grow with 4-hydroxybutyrate, and mutants exhibiting the phenotype 4-hydroxybutyrate-leaky grew at a significantly lower rate with 4-hydroxybutyrate. Hybridization experiments led to the identification of a 10-kbp genomic EcoRI fragment of A. eutrophus SK4040, which was altered in mutants with the phenotype 4-hydroxybutyrate-negative, and of two 1-kbp and 4.5-kbp genomic EcoRI fragments, which were altered in mutants with the phenotype 4-hydroxybutyrate-leaky. This 10-kbp EcoRI fragment was cloned from A. eutrophus SK4040, and conjugative transfer of a pVDZ'2 hybrid plasmid to A. eutrophus H16 conferred the ability to grow with 4-hydroxybutyrate to the wild type. DNA-sequence analysis of this fragment, enzymic analysis of the wild type and of mutants of A. eutrophus as well as of recombinant strains of Escherichia coli led to the identification of a structural gene encoding for a 4-hydroxybutyrate dehydrogenase which was affected by transposon mutagenesis in five of six available 4-hydroxybutyrate-negative mutants. Enzymic studies also provided evidence for the presence of an active succinate-semialdehyde dehydrogenase in 4-hydroxybutyrate-grown cells. This indicated that degradation of 4-hydroxybutyrate occurs via succinate semialdehyde and succinate and that the latter is degraded by the citric acid cycle. NMR studies of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) accumulated from 4-hydroxy [1-13C]butyrate or 4-hydroxy[2-13C]butyrate as substrate gave no evidence for a direct conversion of 4-hydroxybutyrate into 3-hydroxybutyrate and therefore supported the results of enzymic analysis.
Other Papers
Alcaligenes eutrophus, Cupriavidus necator (Ralstonia eutropha) (polyhydroxyalkanoic acid synthase)