Fricke_2008_J.Bacteriol_190_6779

Reference

Title : Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5 - Fricke_2008_J.Bacteriol_190_6779
Author(s) : Fricke WF , Wright MS , Lindell AH , Harkins DM , Baker-Austin C , Ravel J , Stepanauskas R
Ref : Journal of Bacteriology , 190 :6779 , 2008
Abstract :

The increasing occurrence of multidrug-resistant pathogens of clinical and agricultural importance is a global public health concern. While antimicrobial use in human and veterinary medicine is known to contribute to the dissemination of antimicrobial resistance, the impact of microbial communities and mobile resistance genes from the environment in this process is not well understood. Isolated from an industrially polluted aquatic environment, Escherichia coli SMS-3-5 is resistant to a record number of antimicrobial compounds from all major classes, including two front-line fluoroquinolones (ciprofloxacin and moxifloxacin), and in many cases at record-high concentrations. To gain insights into antimicrobial resistance in environmental bacterial populations, the genome of E. coli SMS-3-5 was sequenced and compared to the genome sequences of other E. coli strains. In addition, selected genetic loci from E. coli SMS-3-5 predicted to be involved in antimicrobial resistance were phenotypically characterized. Using recombinant vector clones from shotgun sequencing libraries, resistance to tetracycline, streptomycin, and sulfonamide/trimethoprim was assigned to a single mosaic region on a 130-kb plasmid (pSMS35_130). The remaining plasmid backbone showed similarity to virulence plasmids from avian-pathogenic E. coli (APEC) strains. Individual resistance gene cassettes from pSMS35_130 are conserved among resistant bacterial isolates from multiple phylogenetic and geographic sources. Resistance to quinolones was assigned to several chromosomal loci, mostly encoding transport systems that are also present in susceptible E. coli isolates. Antimicrobial resistance in E. coli SMS-3-5 is therefore dependent both on determinants acquired from a mobile gene pool that is likely available to clinical and agricultural pathogens, as well, and on specifically adapted multidrug efflux systems. The association of antimicrobial resistance with APEC virulence genes on pSMS35_130 highlights the risk of promoting the spread of virulence through the extensive use of antibiotics.

PubMedSearch : Fricke_2008_J.Bacteriol_190_6779
PubMedID: 18708504
Gene_locus related to this paper: ecoli-Aes , ecoli-rutD , ecoli-bioh , ecoli-C0410 , ecoli-dlhh , ecoli-entf , ecoli-fes , ecoli-mhpc , ecoli-pldb , ecoli-ptrb , ecoli-estX , ecoli-yafa , ecoli-yaim , ecoli-ybff , ecoli-ycfp , ecoli-ycjy , ecoli-yeiG , ecoli-YFBB , ecoli-yghX , ecoli-yhet , ecoli-yjfp , ecoli-YNBC , ecoli-ypfh , ecoli-yqia , ecoli-YfhR , ecosm-b1lhu2

Related information

Gene_locus ecoli-Aes    ecoli-rutD    ecoli-bioh    ecoli-C0410    ecoli-dlhh    ecoli-entf    ecoli-fes    ecoli-mhpc    ecoli-pldb    ecoli-ptrb    ecoli-estX    ecoli-yafa    ecoli-yaim    ecoli-ybff    ecoli-ycfp    ecoli-ycjy    ecoli-yeiG    ecoli-YFBB    ecoli-yghX    ecoli-yhet    ecoli-yjfp    ecoli-YNBC    ecoli-ypfh    ecoli-yqia    ecoli-YfhR    ecosm-b1lhu2

Citations formats

Fricke WF, Wright MS, Lindell AH, Harkins DM, Baker-Austin C, Ravel J, Stepanauskas R (2008)
Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5
Journal of Bacteriology 190 :6779

Fricke WF, Wright MS, Lindell AH, Harkins DM, Baker-Austin C, Ravel J, Stepanauskas R (2008)
Journal of Bacteriology 190 :6779