Leopold_2009_Proc.Natl.Acad.Sci.U.S.A_106_8713

Reference

Title : A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis - Leopold_2009_Proc.Natl.Acad.Sci.U.S.A_106_8713
Author(s) : Leopold SR , Magrini V , Holt NJ , Shaikh N , Mardis ER , Cagno J , Ogura Y , Iguchi A , Hayashi T , Mellmann A , Karch H , Besser TE , Sawyer SA , Whittam TS , Tarr PI
Ref : Proc Natl Acad Sci U S A , 106 :8713 , 2009
Abstract :

Single nucleotide polymorphisms (SNPs) in stable genome regions provide durable measurements of species evolution. We systematically identified each SNP in concatenations of all backbone ORFs in 7 newly or previously sequenced evolutionarily instructive pathogenic Escherichia coli O157:H7, O157:H(-), and O55:H7. The 1,113 synonymous SNPs demonstrate emergence of the largest cluster of this pathogen only in the last millennium. Unexpectedly, shared SNPs within circumscribed clusters of organisms suggest severely restricted survival and limited effective population sizes of pathogenic O157:H7, tenuous survival of these organisms in nature, source-sink evolutionary dynamics, or, possibly, a limited number of mutations that confer selective advantage. A single large segment spanning the rfb-gnd gene cluster is the only backbone region convincingly acquired by recombination as O157 emerged from O55. This concatenomic analysis also supports using SNPs to differentiate closely related pathogens for infection control and forensic purposes. However, constrained radiations raise the possibility of making false associations between isolates.

PubMedSearch : Leopold_2009_Proc.Natl.Acad.Sci.U.S.A_106_8713
PubMedID: 19439656
Gene_locus related to this paper: ecoli-Aes , ecoli-rutD , ecoli-bioh , ecoli-dlhh , ecoli-entf , ecoli-fes , ecoli-mhpc , ecoli-pldb , ecoli-ptrb , ecoli-yafa , ecoli-yaim , ecoli-ybff , ecoli-ycfp , ecoli-ycjy , ecoli-yeiG , ecoli-YFBB , ecoli-yghX , ecoli-yhet , ecoli-yjfp , ecoli-YNBC , ecoli-ypfh , ecoli-yqia , ecoli-YfhR , neucr-q7sha3

Related information

Gene_locus ecoli-Aes    ecoli-rutD    ecoli-bioh    ecoli-dlhh    ecoli-entf    ecoli-fes    ecoli-mhpc    ecoli-pldb    ecoli-ptrb    ecoli-yafa    ecoli-yaim    ecoli-ybff    ecoli-ycfp    ecoli-ycjy    ecoli-yeiG    ecoli-YFBB    ecoli-yghX    ecoli-yhet    ecoli-yjfp    ecoli-YNBC    ecoli-ypfh    ecoli-yqia    ecoli-YfhR    neucr-q7sha3

Citations formats

Leopold SR, Magrini V, Holt NJ, Shaikh N, Mardis ER, Cagno J, Ogura Y, Iguchi A, Hayashi T, Mellmann A, Karch H, Besser TE, Sawyer SA, Whittam TS, Tarr PI (2009)
A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis
Proc Natl Acad Sci U S A 106 :8713

Leopold SR, Magrini V, Holt NJ, Shaikh N, Mardis ER, Cagno J, Ogura Y, Iguchi A, Hayashi T, Mellmann A, Karch H, Besser TE, Sawyer SA, Whittam TS, Tarr PI (2009)
Proc Natl Acad Sci U S A 106 :8713