Philipp_1996_Proc.Natl.Acad.Sci.U.S.A_93_3132

Reference

Title : An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae - Philipp_1996_Proc.Natl.Acad.Sci.U.S.A_93_3132
Author(s) : Philipp WJ , Poulet S , Eiglmeier K , Pascopella L , Balasubramanian V , Heym B , Bergh S , Bloom BR , Jacobs WR, Jr. , Cole ST
Ref : Proc Natl Acad Sci U S A , 93 :3132 , 1996
Abstract :

An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage sites for Asn I and Dra I to be positioned on the 4.4-Mb circular chromosome, while, in parallel, clones from two cosmid libraries were ordered into contigs by means of fingerprinting and hybridization mapping. The resultant contig map was readily correlated with the physical map of the genome via the landmarked restriction sites. Over 165 genes and markers were localized on the integrated map, thus enabling comparisons with the leprosy bacillus, Mycobacterium leprae, to be undertaken. Mycobacterial genomes appear to have evolved as mosaic structures since extended segments with conserved gene order and organization are interspersed with different flanking regions. Repetitive sequences and insertion elements are highly abundant in M. tuberculosis, but the distribution of IS6110 is apparently nonrandom.

PubMedSearch : Philipp_1996_Proc.Natl.Acad.Sci.U.S.A_93_3132
PubMedID: 8610181
Gene_locus related to this paper: myctu-Rv0217c , myctu-Rv0220 , myctu-Rv1076 , myctu-Rv1399c , myctu-Rv1400c , myctu-Rv1426c , myctu-Rv2045c , myctu-Rv2284 , myctu-Rv2385 , myctu-Rv2485c , myctu-Rv2970c , myctu-Rv3084 , myctu-Rv3097c , myctu-Rv3487c

Related information

Gene_locus myctu-Rv0217c    myctu-Rv0220    myctu-Rv1076    myctu-Rv1399c    myctu-Rv1400c    myctu-Rv1426c    myctu-Rv2045c    myctu-Rv2284    myctu-Rv2385    myctu-Rv2485c    myctu-Rv2970c    myctu-Rv3084    myctu-Rv3097c    myctu-Rv3487c
Gene_locus_frgt myctu-Rv0217c    myctu-Rv0220    myctu-Rv1076    myctu-Rv1399c    myctu-Rv1400c    myctu-Rv1426c    myctu-Rv2045c    myctu-Rv2284    myctu-Rv2385    myctu-Rv2485c    myctu-Rv2970c    myctu-Rv3084    myctu-Rv3097c    myctu-Rv3487c    myctu-Rv1104

Citations formats

Philipp WJ, Poulet S, Eiglmeier K, Pascopella L, Balasubramanian V, Heym B, Bergh S, Bloom BR, Jacobs WR, Jr., Cole ST (1996)
An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae
Proc Natl Acad Sci U S A 93 :3132

Philipp WJ, Poulet S, Eiglmeier K, Pascopella L, Balasubramanian V, Heym B, Bergh S, Bloom BR, Jacobs WR, Jr., Cole ST (1996)
Proc Natl Acad Sci U S A 93 :3132