Mycobacterium tuberculosis, Mycobacterium bovis, Rv3097c lipY PE-PGRS family protein triglycerol acyl hydrolase esterase/lipase
(from Uniprot) Catalyzes the hydrolysis of both intracellular and extracellular triacylglycerol (TAG). In vitro, can also hydrolyze p-nitrophenyl (pNP) esters with various chain lengths, including pNP-acetate (C2), pNP-butyrate (C4), pNP-caproate (C6), pNP-caprylate (C8), pNP-laurate (C12), pNP-myristate (C14), pNP-palmitate (C16) and pNP-stearate (C18). Also hydrolyzes monobutyrin, tributyrin and trioctanoin. Overexpression results in increase of virulence characterized by reduced survival of infected mouse and increased burden of bacilli in the lungs. Hydrolyzes internal or host-derived TAG depending on its localization. Hydrolyzes TAG that accumulates within mycobacterial intracytosolic lipid inclusions (ILI). Probably responsible for the utilization of stored long-chain TAG during the dormancy and reactivation stages of the pathogen. N-terminal also homologous to PE subfamily of glycine-rich proteins. PE proteins cross the unique cell wall that protects the pathogen from eradication by the immune system through the type VII secretion or ESX systems. PE_PGRS domain is processed by PecA an aspartic protease. There are more than 1000 strains. Other Uniprot entries and list of strains can be found with the link: Other strains.
Family : Hormone-sensitive_lipase_like
Block : H
Position in NCBI Life Tree : Mycobacterium tuberculosis
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Terrabacteria group N E > Actinobacteria [phylum] N E > Actinobacteria [class] N E > Corynebacteriales N E > Mycobacteriaceae N E > Mycobacterium N E > Mycobacterium tuberculosis complex N E > Mycobacterium tuberculosis N EMVSYVVALPE VMSAAATDVA SIGSVVATAS QGVAGATTTV LAAAEDEVSA AIAALFSGHG QDYQALSAQL AVFHERFVQA LTGAAKGYAA AELANASLLQ SEFASGIGNG FATIHQEIQR APTALAAGFT QVPPFAAAQA GIFTGTPSGA AGFDIASLWP VKPLLSLSAL ETHFAIPNNP LLALIASDIP PLSWFLGNSP PPLLNSLLGQ TVQYTTYDGM SVVQITPAHP TGEYVVAIHG GAFILPPSIF HWLNYSVTAY QTGATVQVPI YPLVQEGGTA GTVVPAMAGL ISTQIAQHGV SNVSVVGDSA GGNLALAAAQ YMVSQGNPVP SSMVLLSPWL DVGTWQISQA WAGNLAVNDP LVSPLYGSLN GLPPTYVYSG SLDPLAQQAV VLEHTAVVQG APFSFVLAPW QIHDWILLTP WGLLSWPQIN QQLGIAA
| Title : Dissecting the membrane lipid binding properties and lipase activity of Mycobacterium tuberculosis LipY domains - Santucci_2019_Febs.j_286_3164 |
| Author(s) : Santucci P , Smichi N , Diomande S , Poncin I , Point V , Gaussier H , Cavalier JF , Kremer L , Canaan S |
| Ref : Febs J , 286 :3164 , 2019 |
| Abstract : |
| PubMedSearch : Santucci_2019_Febs.j_286_3164 |
| PubMedID: 31034693 |
| Gene_locus related to this paper: myctu-Rv3097c |
| Title : Type VII Secretion Substrates of Pathogenic Mycobacteria Are Processed by a Surface Protease - Burggraaf_2019_mBio_10_e01951 |
| Author(s) : Burggraaf MJ , Speer A , Meijers AS , Ummels R , van der Sar AM , Korotkov KV , Bitter W , Kuijl C |
| Ref : MBio , 10 :e01951 , 2019 |
| Abstract : |
| PubMedSearch : Burggraaf_2019_mBio_10_e01951 |
| PubMedID: 31662454 |
| Gene_locus related to this paper: myctu-Rv3097c |
| Title : Delineating the Physiological Roles of the PE and Catalytic Domains of LipY in Lipid Consumption in Mycobacterium-Infected Foamy Macrophages - Santucci_2018_Infect.Immun_86_e00394 |
| Author(s) : Santucci P , Diomande S , Poncin I , Alibaud L , Viljoen A , Kremer L , de Chastellier C , Canaan S |
| Ref : Infect Immun , 86 : , 2018 |
| Abstract : |
| PubMedSearch : Santucci_2018_Infect.Immun_86_e00394 |
| PubMedID: 29986895 |
| Gene_locus related to this paper: myctu-Rv3097c |
| Title : Modulation of the Activity of Mycobacterium tuberculosis LipY by Its PE Domain - Garrett_2015_PLoS.One_10_e0135447 |
| Author(s) : Garrett CK , Broadwell LJ , Hayne CK , Neher SB |
| Ref : PLoS ONE , 10 :e0135447 , 2015 |
| Abstract : |
| PubMedSearch : Garrett_2015_PLoS.One_10_e0135447 |
| PubMedID: 26270534 |
| Gene_locus related to this paper: myctu-Rv3097c |
| Title : Identification and Characterization of Lipase Activity and Immunogenicity of LipL from Mycobacterium tuberculosis - Cao_2015_PLoS.One_10_e0138151 |
| Author(s) : Cao J , Dang G , Li H , Li T , Yue Z , Li N , Liu Y , Liu S , Chen L |
| Ref : PLoS ONE , 10 :e0138151 , 2015 |
| Abstract : |
| PubMedSearch : Cao_2015_PLoS.One_10_e0138151 |
| PubMedID: 26398213 |
| Gene_locus related to this paper: myctu-Rv1076 , myctu-Rv2485c , myctu-Rv3097c |
| Title : Increased virulence of Mycobacterium tuberculosis H37Rv overexpressing LipY in a murine model - Singh_2014_Tuberculosis.(Edinb)_94_252 |
| Author(s) : Singh VK , Srivastava M , Dasgupta A , Singh MP , Srivastava R , Srivastava BS |
| Ref : Tuberculosis (Edinb) , 94 :252 , 2014 |
| Abstract : |
| PubMedSearch : Singh_2014_Tuberculosis.(Edinb)_94_252 |
| PubMedID: 24631199 |
| Gene_locus related to this paper: myctu-Rv3097c |
| Title : Identification and characterisation of small-molecule inhibitors of Rv3097c-encoded lipase (LipY) of Mycobacterium tuberculosis that selectively inhibit growth of bacilli in hypoxia - Saxena_2013_Int.J.Antimicrob.Agents_42_27 |
| Author(s) : Saxena AK , Roy KK , Singh S , Vishnoi SP , Kumar A , Kashyap VK , Kremer L , Srivastava R , Srivastava BS |
| Ref : Int J Antimicrob Agents , 42 :27 , 2013 |
| Abstract : |
| PubMedSearch : Saxena_2013_Int.J.Antimicrob.Agents_42_27 |
| PubMedID: 23684389 |
| Gene_locus related to this paper: myctu-Rv3097c |
| Title : Conserved Pro-Glu (PE) and Pro-Pro-Glu (PPE) protein domains target LipY lipases of pathogenic mycobacteria to the cell surface via the ESX-5 pathway - Daleke_2011_J.Biol.Chem_286_19024 |
| Author(s) : Daleke MH , Cascioferro A , de Punder K , Ummels R , Abdallah AM , van der Wel N , Peters PJ , Luirink J , Manganelli R , Bitter W |
| Ref : Journal of Biological Chemistry , 286 :19024 , 2011 |
| Abstract : |
| PubMedSearch : Daleke_2011_J.Biol.Chem_286_19024 |
| PubMedID: 21471225 |
| Gene_locus related to this paper: myctu-Rv3097c |
| Title : TubercuList--10 years after - Lew_2011_Tuberculosis_91_1 |
| Author(s) : Lew JM , Kapopoulou A , Jones LM , Cole ST |
| Ref : Tuberculosis (Edinb) , 91 :1 , 2011 |
| Abstract : |
| PubMedSearch : Lew_2011_Tuberculosis_91_1 |
| PubMedID: 20980199 |
| Gene_locus related to this paper: myctu-ephA , myctu-ephB , myctu-LPQP , myctu-Rv0160c , myctu-Rv2485c , myctu-RV2695 , myctu-RV2765 , myctu-RV2800 , myctu-Rv3097c |
| Title : Functional role of the PE domain and immunogenicity of the Mycobacterium tuberculosis triacylglycerol hydrolase LipY - Mishra_2008_Infect.Immun_76_127 |
| Author(s) : Mishra KC , de Chastellier C , Narayana Y , Bifani P , Brown AK , Besra GS , Katoch VM , Joshi B , Balaji KN , Kremer L |
| Ref : Infect Immun , 76 :127 , 2008 |
| Abstract : |
| PubMedSearch : Mishra_2008_Infect.Immun_76_127 |
| PubMedID: 17938218 |
| Gene_locus related to this paper: myctu-Rv3097c |
| Title : A novel lipase belonging to the hormone-sensitive lipase family induced under starvation to utilize stored triacylglycerol in Mycobacterium tuberculosis - Deb_2006_J.Biol.Chem_281_3866 |
| Author(s) : Deb C , Daniel J , Sirakova TD , Abomoelak B , Dubey VS , Kolattukudy PE |
| Ref : Journal of Biological Chemistry , 281 :3866 , 2006 |
| Abstract : |
| PubMedSearch : Deb_2006_J.Biol.Chem_281_3866 |
| PubMedID: 16354661 |
| Gene_locus related to this paper: myctu-Rv3097c |
| Title : Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv - Camus_2002_Microbiology_148_2967 |
| Author(s) : Camus JC , Pryor MJ , Medigue C , Cole ST |
| Ref : Microbiology , 148 :2967 , 2002 |
| Abstract : |
| PubMedSearch : Camus_2002_Microbiology_148_2967 |
| PubMedID: 12368430 |
| Gene_locus related to this paper: myctu-d5yk66 , myctu-ephA , myctu-ephB , myctu-LPQP , myctu-p71654 , myctu-Rv0160c , myctu-RV0774C , myctu-Rv2485c , myctu-RV2672 , myctu-RV2695 , myctu-RV2765 , myctu-RV2800 , myctu-Rv2970c , myctu-Rv3084 , myctu-Rv3097c , myctu-RV3452 |
| Title : An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae - Philipp_1996_Proc.Natl.Acad.Sci.U.S.A_93_3132 |
| Author(s) : Philipp WJ , Poulet S , Eiglmeier K , Pascopella L , Balasubramanian V , Heym B , Bergh S , Bloom BR , Jacobs WR, Jr. , Cole ST |
| Ref : Proc Natl Acad Sci U S A , 93 :3132 , 1996 |
| Abstract : |
| PubMedSearch : Philipp_1996_Proc.Natl.Acad.Sci.U.S.A_93_3132 |
| PubMedID: 8610181 |
| Gene_locus related to this paper: myctu-Rv0217c , myctu-Rv0220 , myctu-Rv1076 , myctu-Rv1399c , myctu-Rv1400c , myctu-Rv1426c , myctu-Rv2045c , myctu-Rv2284 , myctu-Rv2385 , myctu-Rv2485c , myctu-Rv2970c , myctu-Rv3084 , myctu-Rv3097c , myctu-Rv3487c |