HAA-synthase-thioesterase-RhlA-PhaG
Relationship
Comment
The biosurfactant 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA) is the hydrophobic moiety of rhamnolipids and most often consists of two hydroxy fatty acids linked by an ester bond. rhlA genes encoding the acyltransferase responsible for the synthesis of the lipophilic rhamnolipid precursor 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA) (found only in Betaproteobacteria and Gammaproteobacteria or by horizontal gene transfer in Actinobacteria). Acyl-carrier protein (ACP)-activated hydroxy fatty acids are the preferred substrate for RhlA. The carbon chain lengths of HAAs determine their abilities to foam and emulsify, and their critical micelle concentration (CMC). Their chain lengths are determined by RhlA structure. The family alos contains phaG genes involved in polyhydroxyalkanoate biosynthesis. 3-hydroxyacyl-acyl carrier protein CoA transferase involved in the synthesis of polyhydroxyalkanoic acid with medium chain length
Database
Interpro : No interpro
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : No Pfam
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 8IK2 | RhlA exhibits dual thioesterase and acyltransferase activities during rhamnolipid biosynthesis | pseae-rhla |
| Gene_locus | Name | Species |
|---|---|---|
| 9psed-b7sjg2 | Pseudomonas sp. USM 4-55 R-3-hydroxyacyl-ACP:CoA transferase | Pseudomonas sp. USM 4-55 |
| 9psed-i4n2z6 | Pseudomonas sp. M47T1. Rhamnosyltransferase chain A | Pseudomonas sp. M47T1 |
| burca-PHAG | Burkholderia caryophylli hydroxyacyl-acp:coa transacylase phag | Burkholderia caryophylli |
| burcj-b4ehi9 | Burkholderia cepacia, Burkholderia cenocepacia, Burkholderia sp., Burkholderia cepacia, Putative (R)-3-hydroxydecanoyl-ACP:CoA transacylase | Burkholderia cepacia |
| burcm-q0b714 | Burkholderia ambifaria (strains ATCC BAA-244 \/ AMMD\; MEX-5\; MC40-6\; IOP40-10) (Burkholderia cepacia (strain AMMD)) Alpha\/beta hydrolase fold | Burkholderia ambifaria |
| burgb-c5amf7 | Burkholderia glumae (strain BGR1) Rhamnosyltransferase 1, subunit A | Burkholderia glumae |
| burps-q3v7s4 | Burkholderia pseudomallei (Pseudomonas pseudomallei), Burkholderia mallei (Pseudomonas mallei) Putative fatty acid biosynthetic protein | Burkholderia pseudomallei |
| burta-q2t424 | Burkholderia thailandensis (strain E264 \/ ATCC 700388 \/ DSM 13276 CIP 106301) Rhamnosyltransferase 1, subunit A | Burkholderia thailandensis |
| dicd5-d2c1p1 | Dickeya dadantii (strain Ech586) Alpha\/beta hydrolase fold protein | Dickeya dadantii |
| dicdc-c6c8b4 | Dickeya dadantii (strain Ech703) Alpha\/beta hydrolase fold protein | Dickeya dadantii |
| dicze-c6ckc2 | Dickeya zeae (strain Ech1591) Alpha\/beta hydrolase fold protein | Dickeya zeae |
| halnc-d0kwx9 | Halothiobacillus neapolitanus (strain ATCC 23641 \/ c2) (Thiobacillus neapolitanus) 3-hydroxyacyl-CoA-acyl carrier protein transferase | Halothiobacillus neapolitanus |
| panam-d4gk92 | Pantoea ananatis (strain LMG 20103) RhlA | Pantoea ananatis |
| pse14-q48es1 | Pseudomonas syringae pv. phaseolicola (strain 1448A \/ Race 6) 3-hydroxyacyl-CoA-acyl carrier protein transferase (EC 2.4.1.-) | Pseudomonas syringae pv. phaseolicola |
| pse14-q48hb4 | Pseudomonas syringae pv. phaseolicola (strain 1448A \/ Race 6) 3-hydroxyacyl-CoA-acyl carrier protein transferase (EC 2.4.1.-) | Pseudomonas syringae pv. phaseolicola |
| psea4-u3aua5 | Pseudomonas alcaligenes (strain ATCC 14909 \/ DSM 50342 \/ JCM 20561 \/ NBRC 14159 \/ NCIMB 9945 \/ NCTC 10367 \/ 1577). Putative hydrolase | Pseudomonas alcaligenes |
| pseae-phag | Pseudomonas aeruginosa 3-hydroxyacyl-CoA-acyl carrier protein transferase. Quinolone sensitivity protein phaG qin PA0730 | Pseudomonas aeruginosa |
| pseae-rhla |  Pseudomonas aeruginosa, Pseudomonas sp., rhamnosyltransferase 1 subunit a (EC 2.4.1.-) RhlA |
Pseudomonas aeruginosa |
| psee4-q1i5s9 | Pseudomonas entomophila (strain L48) (R)-3-hydroxydecanoyl-ACP:CoA transacylase (3-hydroxyacyl-CoA-acyl carrier protein transferase) | Pseudomonas entomophila |
| psef1-f6aax1 | Pseudomonas fulva (strain 12-X). Alpha\/beta hydrolase fold protein | Pseudomonas fulva |
| psef5-q4kgs6 | Pseudomonas fluorescens (strain Pf-5 \/ ATCC BAA-477) 3-hydroxyacyl-CoA-acyl carrier protein transferase | Pseudomonas fluorescens |
| psefl-b1pwe2 | Pseudomonas fluorescens 3-hydroxyacyl-CoA:ACP acyl transferase | Pseudomonas fluorescens |
| psefs-c3k3d6 | Pseudomonas fluorescens (strain SBW25) Rhamnosyltransferase 1 subunit A | Pseudomonas fluorescens |
| psefs-c3kdh9 | Pseudomonas fluorescens (strain SBW25) (R)-3-hydroxydecanoyl-ACP:CoA transacylase | Pseudomonas fluorescens |
| psemy-a4xs03 | Pseudomonas mendocina (strain ymp) Alpha\/beta hydrolase fold protein | Pseudomonas mendocina |
| pseol-PHAG | Pseudomonas oleovorans transacylase phag | Pseudomonas oleovorans |
| psepf-q3kgm9 | Pseudomonas fluorescens (strain PfO-1) alpha\/beta hydrolase fold | Pseudomonas fluorescens |
| psepu-PHAG | Pseudomonas putida, Arthrobacter siderocapsulatus, P. nitroreducens, P. pseudoalcaligenes (stutzeri, mendocina perfectomarina) acyl-transferase (phaG) gene, complete cds | Pseudomonas putida |
| psere-s6agn4 | Pseudomonas resinovorans NBRC 106553. (R)-3-hydroxydecanoyl-ACP--CoA transacylase | Pseudomonas resinovorans NBRC 106553 |
| psesp-PHAG | Pseudomonas sp. 61-3 3-hydroxyacyl-acyl carrier protein coa transferase | Pseudomonas sp. 61-3 |
| psesy-PHAG1 | Pseudomonas syringae (pv. tomato) 3-hydroxyacyl-CoA-acyl carrier protein transferase | Pseudomonas syringae |
| psesy-PHAG2 | Pseudomonas syringae (pv. tomato) 3-hydroxyacyl-CoA-acyl carrier protein transferase | Pseudomonas syringae |
| pseu2-q4zpb0 | Pseudomonas syringae pv. syringae (strain B728a) 3-hydroxyacyl-CoA-acyl carrier protein transferase | Pseudomonas syringae pv. syringae |
| pseu2-q4zrr1 | Pseudomonas syringae pv. syringae (strain B728a) 3-hydroxyacyl-CoA-acyl carrier protein transferase | Pseudomonas syringae pv. syringae |
| serod-d4e5a8 | Serratia odorifera DSM 4582 3-hydroxyacyl-CoA-acyl carrier protein transferase | Serratia odorifera DSM 4582 |
| sers3-a0a2i5t9g2 | Serratia sp. (strain ATCC 39006)3-hydroxyacyl-CoA-acyl-carrier protein transferase RhlA | Serratia sp. ATCC 39006 |
| Substrate | Chemical Nomenclature | Proteins |
|---|---|---|
| 3-Hydroxydecanoyl-3-hydroxydecanoic-acid | 3-(3-hydroxydecanoyloxy)decanoic acid | pseae-rhla |
| 3-hydroxydecanoic-acid | (3S)-3-hydroxydecanoic acid | psere-PHAC1 |
References (10)
| Title : RhlA Exhibits Dual Thioesterase and Acyltransferase Activities during Rhamnolipid Biosynthesis - Tang_2023_ACS.Catal_13_5759 |
| Author(s) : Ting Tang T , Fu L , Xie W , Luo Y , Zhang Y , Zhang J , Si T |
| Ref : ACS Catal , 13 :5759 , 2023 |
| Abstract : |
| PubMedSearch : Tang_2023_ACS.Catal_13_5759 |
| PubMedID: |
| Gene_locus related to this paper: pseae-rhla |
| Title : Exploiting the Natural Diversity of RhlA Acyltransferases for the Synthesis of the Rhamnolipid Precursor 3-(3-Hydroxyalkanoyloxy)Alkanoic Acid - Germer_2020_Appl.Environ.Microbiol_86_e02317 |
| Author(s) : Germer A , Tiso T , Muller C , Behrens B , Vosse C , Scholz K , Froning M , Hayen H , Blank LM |
| Ref : Applied Environmental Microbiology , 86 : , 2020 |
| Abstract : |
| PubMedSearch : Germer_2020_Appl.Environ.Microbiol_86_e02317 |
| PubMedID: 31924623 |
| Gene_locus related to this paper: pseae-rhla |
| Title : Oil degradation and biosurfactant production by the deep sea bacterium Dietzia maris As-13-3 - Wang_2014_Front.Microbiol_5_711 |
| Author(s) : Wang W , Cai B , Shao Z |
| Ref : Front Microbiol , 5 :711 , 2014 |
| Abstract : |
| PubMedSearch : Wang_2014_Front.Microbiol_5_711 |
| PubMedID: 25566224 |
| Gene_locus related to this paper: burcj-b4ehi9 |
| Title : RhlA converts beta-hydroxyacyl-acyl carrier protein intermediates in fatty acid synthesis to the beta-hydroxydecanoyl-beta-hydroxydecanoate component of rhamnolipids in Pseudomonas aeruginosa - Zhu_2008_J.Bacteriol_190_3147 |
| Author(s) : Zhu K , Rock CO |
| Ref : Journal of Bacteriology , 190 :3147 , 2008 |
| Abstract : |
| PubMedSearch : Zhu_2008_J.Bacteriol_190_3147 |
| PubMedID: 18326581 |
| Gene_locus related to this paper: pseae-rhla |
| Title : Poly(hydroxyalkanoate) synthase genotype and PHA production of Pseudomonas corrugata and P. mediterranea - Solaiman_2005_J.Ind.Microbiol.Biotechnol_32_75 |
| Author(s) : Solaiman DK , Catara V , Greco S |
| Ref : J Ind Microbiol Biotechnol , 32 :75 , 2005 |
| Abstract : |
| PubMedSearch : Solaiman_2005_J.Ind.Microbiol.Biotechnol_32_75 |
| PubMedID: 15726440 |
| Title : Production of rhamnolipids by Pseudomonas chlororaphis, a nonpathogenic bacterium - Gunther_2005_Appl.Environ.Microbiol_71_2288 |
| Author(s) : Gunther NWt , Nunez A , Fett W , Solaiman DK |
| Ref : Applied Environmental Microbiology , 71 :2288 , 2005 |
| Abstract : |
| PubMedSearch : Gunther_2005_Appl.Environ.Microbiol_71_2288 |
| PubMedID: 15870313 |
| Title : Biosynthesis of medium-chain-length poly(hydroxyalkanoates) with altered composition by mutant hybrid PHA synthases - Solaiman_2003_J.Ind.Microbiol.Biotechnol_30_322 |
| Author(s) : Solaiman DK |
| Ref : J Ind Microbiol Biotechnol , 30 :322 , 2003 |
| Abstract : |
| PubMedSearch : Solaiman_2003_J.Ind.Microbiol.Biotechnol_30_322 |
| PubMedID: 12761653 |
| Gene_locus related to this paper: psere-PHAC1 |
| Title : The Pseudomonas aeruginosa phaG gene product is involved in the synthesis of polyhydroxyalkanoic acid consisting of medium-chain-length constituents from non-related carbon sources - Hoffmann_2000_FEMS.Microbiol.Lett_184_253 |
| Author(s) : Hoffmann N , Steinbuchel A , Rehm BH |
| Ref : FEMS Microbiology Letters , 184 :253 , 2000 |
| Abstract : |
| PubMedSearch : Hoffmann_2000_FEMS.Microbiol.Lett_184_253 |
| PubMedID: 10713430 |
| Gene_locus related to this paper: pseae-phag |
| Title : A new metabolic link between fatty acid de novo synthesis and polyhydroxyalkanoic acid synthesis. The PHAG gene from Pseudomonas putida KT2440 encodes a 3-hydroxyacyl-acyl carrier protein-coenzyme a transferase - Rehm_1998_J.Biol.Chem_273_24044 |
| Author(s) : Rehm BH , Kruger N , Steinbuchel A |
| Ref : Journal of Biological Chemistry , 273 :24044 , 1998 |
| Abstract : |
| PubMedSearch : Rehm_1998_J.Biol.Chem_273_24044 |
| PubMedID: 9727022 |
| Gene_locus related to this paper: psepu-PHAG |
| Title : Isolation, characterization, and expression in Escherichia coli of the Pseudomonas aeruginosa rhlAB genes encoding a rhamnosyltransferase involved in rhamnolipid biosurfactant synthesis - Ochsner_1994_J.Biol.Chem_269_19787 |
| Author(s) : Ochsner UA , Fiechter A , Reiser J |
| Ref : Journal of Biological Chemistry , 269 :19787 , 1994 |
| Abstract : |
| PubMedSearch : Ochsner_1994_J.Biol.Chem_269_19787 |
| PubMedID: 8051059 |
| Gene_locus related to this paper: pseae-rhla |