HAA-synthase-thioesterase-RhlA-PhaG

Relationship

Family: HAA-synthase-thioesterase-RhlA-PhaG

Block: X

Parent Family: Abhydrolase_6

Comment

The biosurfactant 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA) is the hydrophobic moiety of rhamnolipids and most often consists of two hydroxy fatty acids linked by an ester bond. rhlA genes encoding the acyltransferase responsible for the synthesis of the lipophilic rhamnolipid precursor 3-(3-hydroxyalkanoyloxy)alkanoic acid (HAA) (found only in Betaproteobacteria and Gammaproteobacteria or by horizontal gene transfer in Actinobacteria). Acyl-carrier protein (ACP)-activated hydroxy fatty acids are the preferred substrate for RhlA. The carbon chain lengths of HAAs determine their abilities to foam and emulsify, and their critical micelle concentration (CMC). Their chain lengths are determined by RhlA structure. The family alos contains phaG genes involved in polyhydroxyalkanoate biosynthesis. 3-hydroxyacyl-acyl carrier protein CoA transferase involved in the synthesis of polyhydroxyalkanoic acid with medium chain length

Database

Interpro : No interpro

PIRSF : No PIRSF

Pdoc : No Pdoc

Pfam : No Pfam

Prints : No Print

EC Number : No EC Number

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
8IK2 RhlA exhibits dual thioesterase and acyltransferase activities during rhamnolipid biosynthesis pseae-rhla

Gene_locus Name Species
sers3-a0a2i5t9g2 Serratia sp. (strain ATCC 39006)3-hydroxyacyl-CoA-acyl-carrier protein transferase RhlA Serratia sp. ATCC 39006
9psed-b7sjg2 Pseudomonas sp. USM 4-55 R-3-hydroxyacyl-ACP:CoA transferase Pseudomonas sp. USM 4-55
burca-PHAG Burkholderia caryophylli hydroxyacyl-acp:coa transacylase phag Burkholderia caryophylli
burcj-b4ehi9 Burkholderia cepacia, Burkholderia cenocepacia, Burkholderia sp., Burkholderia cepacia, Putative (R)-3-hydroxydecanoyl-ACP:CoA transacylase Burkholderia cepacia
burcm-q0b714 Burkholderia ambifaria (strains ATCC BAA-244 \/ AMMD\; MEX-5\; MC40-6\; IOP40-10) (Burkholderia cepacia (strain AMMD)) Alpha\/beta hydrolase fold Burkholderia ambifaria
burgb-c5amf7 Burkholderia glumae (strain BGR1) Rhamnosyltransferase 1, subunit A Burkholderia glumae
burps-q3v7s4 Burkholderia pseudomallei (Pseudomonas pseudomallei), Burkholderia mallei (Pseudomonas mallei) Putative fatty acid biosynthetic protein Burkholderia pseudomallei
burta-q2t424 Burkholderia thailandensis (strain E264 \/ ATCC 700388 \/ DSM 13276 CIP 106301) Rhamnosyltransferase 1, subunit A Burkholderia thailandensis
dicd5-d2c1p1 Dickeya dadantii (strain Ech586) Alpha\/beta hydrolase fold protein Dickeya dadantii
dicdc-c6c8b4 Dickeya dadantii (strain Ech703) Alpha\/beta hydrolase fold protein Dickeya dadantii
dicze-c6ckc2 Dickeya zeae (strain Ech1591) Alpha\/beta hydrolase fold protein Dickeya zeae
halnc-d0kwx9 Halothiobacillus neapolitanus (strain ATCC 23641 \/ c2) (Thiobacillus neapolitanus) 3-hydroxyacyl-CoA-acyl carrier protein transferase Halothiobacillus neapolitanus
panam-d4gk92 Pantoea ananatis (strain LMG 20103) RhlA Pantoea ananatis
pse14-q48es1 Pseudomonas syringae pv. phaseolicola (strain 1448A \/ Race 6) 3-hydroxyacyl-CoA-acyl carrier protein transferase (EC 2.4.1.-) Pseudomonas syringae pv. phaseolicola
pse14-q48hb4 Pseudomonas syringae pv. phaseolicola (strain 1448A \/ Race 6) 3-hydroxyacyl-CoA-acyl carrier protein transferase (EC 2.4.1.-) Pseudomonas syringae pv. phaseolicola
pseae-phag Pseudomonas aeruginosa 3-hydroxyacyl-CoA-acyl carrier protein transferase. Quinolone sensitivity protein phaG qin PA0730 Pseudomonas aeruginosa
pseae-rhla Pseudomonas aeruginosa, Pseudomonas sp., rhamnosyltransferase 1 subunit a (EC 2.4.1.-) RhlA Pseudomonas aeruginosa
psee4-q1i5s9 Pseudomonas entomophila (strain L48) (R)-3-hydroxydecanoyl-ACP:CoA transacylase (3-hydroxyacyl-CoA-acyl carrier protein transferase) Pseudomonas entomophila
psef5-q4kgs6 Pseudomonas fluorescens (strain Pf-5 \/ ATCC BAA-477) 3-hydroxyacyl-CoA-acyl carrier protein transferase Pseudomonas fluorescens
psefl-b1pwe2 Pseudomonas fluorescens 3-hydroxyacyl-CoA:ACP acyl transferase Pseudomonas fluorescens
psefs-c3k3d6 Pseudomonas fluorescens (strain SBW25) Rhamnosyltransferase 1 subunit A Pseudomonas fluorescens
psefs-c3kdh9 Pseudomonas fluorescens (strain SBW25) (R)-3-hydroxydecanoyl-ACP:CoA transacylase Pseudomonas fluorescens
psemy-a4xs03 Pseudomonas mendocina (strain ymp) Alpha\/beta hydrolase fold protein Pseudomonas mendocina
pseol-PHAG Pseudomonas oleovorans transacylase phag Pseudomonas oleovorans
psepf-q3kgm9 Pseudomonas fluorescens (strain PfO-1) alpha\/beta hydrolase fold Pseudomonas fluorescens
psepu-PHAG Pseudomonas putida, Arthrobacter siderocapsulatus, P. nitroreducens, P. pseudoalcaligenes (stutzeri, mendocina perfectomarina) acyl-transferase (phaG) gene, complete cds Pseudomonas putida
psesp-PHAG Pseudomonas sp. 61-3 3-hydroxyacyl-acyl carrier protein coa transferase Pseudomonas sp. 61-3
psesy-PHAG1 Pseudomonas syringae (pv. tomato) 3-hydroxyacyl-CoA-acyl carrier protein transferase Pseudomonas syringae
psesy-PHAG2 Pseudomonas syringae (pv. tomato) 3-hydroxyacyl-CoA-acyl carrier protein transferase Pseudomonas syringae
pseu2-q4zpb0 Pseudomonas syringae pv. syringae (strain B728a) 3-hydroxyacyl-CoA-acyl carrier protein transferase Pseudomonas syringae pv. syringae
pseu2-q4zrr1 Pseudomonas syringae pv. syringae (strain B728a) 3-hydroxyacyl-CoA-acyl carrier protein transferase Pseudomonas syringae pv. syringae
serod-d4e5a8 Serratia odorifera DSM 4582 3-hydroxyacyl-CoA-acyl carrier protein transferase Serratia odorifera DSM 4582
psef1-f6aax1 Pseudomonas fulva (strain 12-X). Alpha\/beta hydrolase fold protein Pseudomonas fulva
9psed-i4n2z6 Pseudomonas sp. M47T1. Rhamnosyltransferase chain A Pseudomonas sp. M47T1
psere-s6agn4 Pseudomonas resinovorans NBRC 106553. (R)-3-hydroxydecanoyl-ACP--CoA transacylase Pseudomonas resinovorans NBRC 106553
psea4-u3aua5 Pseudomonas alcaligenes (strain ATCC 14909 \/ DSM 50342 \/ JCM 20561 \/ NBRC 14159 \/ NCIMB 9945 \/ NCTC 10367 \/ 1577). Putative hydrolase Pseudomonas alcaligenes

Substrate Chemical Nomenclature Proteins
3-Hydroxydecanoyl-3-hydroxydecanoic-acid 3-(3-hydroxydecanoyloxy)decanoic acid pseae-rhla
3-hydroxydecanoic-acid (3S)-3-hydroxydecanoic acid psere-PHAC1

References (10)

Title : RhlA Exhibits Dual Thioesterase and Acyltransferase Activities during Rhamnolipid Biosynthesis - Tang_2023_ACS.Catal_13_5759
Author(s) : Ting Tang T , Fu L , Xie W , Luo Y , Zhang Y , Zhang J , Si T
Ref : ACS Catal , 13 :5759 , 2023
Abstract :
PubMedSearch : Tang_2023_ACS.Catal_13_5759
PubMedID:
Gene_locus related to this paper: pseae-rhla

Title : Exploiting the Natural Diversity of RhlA Acyltransferases for the Synthesis of the Rhamnolipid Precursor 3-(3-Hydroxyalkanoyloxy)Alkanoic Acid - Germer_2020_Appl.Environ.Microbiol_86_e02317
Author(s) : Germer A , Tiso T , Muller C , Behrens B , Vosse C , Scholz K , Froning M , Hayen H , Blank LM
Ref : Applied Environmental Microbiology , 86 : , 2020
Abstract :
PubMedSearch : Germer_2020_Appl.Environ.Microbiol_86_e02317
PubMedID: 31924623
Gene_locus related to this paper: pseae-rhla

Title : Oil degradation and biosurfactant production by the deep sea bacterium Dietzia maris As-13-3 - Wang_2014_Front.Microbiol_5_711
Author(s) : Wang W , Cai B , Shao Z
Ref : Front Microbiol , 5 :711 , 2014
Abstract :
PubMedSearch : Wang_2014_Front.Microbiol_5_711
PubMedID: 25566224
Gene_locus related to this paper: burcj-b4ehi9

Title : RhlA converts beta-hydroxyacyl-acyl carrier protein intermediates in fatty acid synthesis to the beta-hydroxydecanoyl-beta-hydroxydecanoate component of rhamnolipids in Pseudomonas aeruginosa - Zhu_2008_J.Bacteriol_190_3147
Author(s) : Zhu K , Rock CO
Ref : Journal of Bacteriology , 190 :3147 , 2008
Abstract :
PubMedSearch : Zhu_2008_J.Bacteriol_190_3147
PubMedID: 18326581
Gene_locus related to this paper: pseae-rhla

Title : Poly(hydroxyalkanoate) synthase genotype and PHA production of Pseudomonas corrugata and P. mediterranea - Solaiman_2005_J.Ind.Microbiol.Biotechnol_32_75
Author(s) : Solaiman DK , Catara V , Greco S
Ref : J Ind Microbiol Biotechnol , 32 :75 , 2005
Abstract :
PubMedSearch : Solaiman_2005_J.Ind.Microbiol.Biotechnol_32_75
PubMedID: 15726440

Title : Production of rhamnolipids by Pseudomonas chlororaphis, a nonpathogenic bacterium - Gunther_2005_Appl.Environ.Microbiol_71_2288
Author(s) : Gunther NWt , Nunez A , Fett W , Solaiman DK
Ref : Applied Environmental Microbiology , 71 :2288 , 2005
Abstract :
PubMedSearch : Gunther_2005_Appl.Environ.Microbiol_71_2288
PubMedID: 15870313

Title : Biosynthesis of medium-chain-length poly(hydroxyalkanoates) with altered composition by mutant hybrid PHA synthases - Solaiman_2003_J.Ind.Microbiol.Biotechnol_30_322
Author(s) : Solaiman DK
Ref : J Ind Microbiol Biotechnol , 30 :322 , 2003
Abstract :
PubMedSearch : Solaiman_2003_J.Ind.Microbiol.Biotechnol_30_322
PubMedID: 12761653
Gene_locus related to this paper: psere-PHAC1

Title : The Pseudomonas aeruginosa phaG gene product is involved in the synthesis of polyhydroxyalkanoic acid consisting of medium-chain-length constituents from non-related carbon sources - Hoffmann_2000_FEMS.Microbiol.Lett_184_253
Author(s) : Hoffmann N , Steinbuchel A , Rehm BH
Ref : FEMS Microbiology Letters , 184 :253 , 2000
Abstract :
PubMedSearch : Hoffmann_2000_FEMS.Microbiol.Lett_184_253
PubMedID: 10713430
Gene_locus related to this paper: pseae-phag

Title : A new metabolic link between fatty acid de novo synthesis and polyhydroxyalkanoic acid synthesis. The PHAG gene from Pseudomonas putida KT2440 encodes a 3-hydroxyacyl-acyl carrier protein-coenzyme a transferase - Rehm_1998_J.Biol.Chem_273_24044
Author(s) : Rehm BH , Kruger N , Steinbuchel A
Ref : Journal of Biological Chemistry , 273 :24044 , 1998
Abstract :
PubMedSearch : Rehm_1998_J.Biol.Chem_273_24044
PubMedID: 9727022
Gene_locus related to this paper: psepu-PHAG

Title : Isolation, characterization, and expression in Escherichia coli of the Pseudomonas aeruginosa rhlAB genes encoding a rhamnosyltransferase involved in rhamnolipid biosurfactant synthesis - Ochsner_1994_J.Biol.Chem_269_19787
Author(s) : Ochsner UA , Fiechter A , Reiser J
Ref : Journal of Biological Chemistry , 269 :19787 , 1994
Abstract :
PubMedSearch : Ochsner_1994_J.Biol.Chem_269_19787
PubMedID: 8051059
Gene_locus related to this paper: pseae-rhla