(The family Pha_synthase was split in four families (PHA_synth_I, PHA_synth_II, PHA_synth_III, PhaC_cen_dom ) according to Interpro and Tigrpfam PhaC_cen_dom groups enzyme with the central domain but not the class I,II,III domains). This entry represents the class II subfamily of poly(R)-hydroxyalkanoate synthases, which polymerises hydroxyacyl-CoAs, typically with six to fourteen carbons in the hydroxyacyl backbone into aliphatic esters termed poly(R)-hydroxyalkanoic acids. These polymers accumulate as carbon and energy storage inclusions in many species and can amount to 90 percent of the dry weight of cell. Poly-beta-hydroxybutyrate polymerase (PhaC) N-terminus. This entry represents the central domain of the bacterial poly-beta-hydroxybutyrate polymerase (PhaC). Polyhydroxyalkanoic acids (PHAs) are carbon and energy reserve polymers produced in some bacteria when carbon sources are plentiful and another nutrient, such as nitrogen, phosphate, oxygen, or sulphur, becomes limiting. PHAs composed of monomeric units ranging from 3 to 14 carbons exist in nature. When the carbon source is exhausted, PHA is utilised by the bacterium. PhaC links D-(-)-3-hydroxybutyrl-CoA to an existing PHA molecule by the formation of an ester bond. PHA_synth_I TIGR01838 PHA_synth_II TIGR01839. The nucleophilic residue of the catalytic triad is a cysteine (with exceptions)
Interpro : IPR010941 PhaC_cen_dom Poly-beta-hydroxybutyrate polymerase, central domain , IPR011287 Poly(R)-hydroxyalkanoic acid synthase, class II
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF07167 PhaC_N
Prints : No Print
EC Number : No EC Number
Peptide in | Fasta |
Nucleotide in | Fasta |
Alignment with Multalin | Text only |
Seed alignment with MAFFT | No colour / coloured with Mview |
Alignment with MAFFT | No colour / coloured with Mview |
Dendrogram | The dnd file |
Gene_locus | Name | Species |
---|---|---|
9psed-q2nm22 | Pseudomonas corrugata pha synthase 2 | Pseudomonas corrugata |
9psed-q2nm24 | Pseudomonas corrugata pha synthase 1 | Pseudomonas corrugata |
9psed-q4pkd1 | Pseudomonas sp. LDC-25 polyhydroxyalkanoate polymerase | Pseudomonas sp. LDC-25 |
9psed-q5d4z0 | Pseudomonas sp. LDC-5 pha synthase 1 | Pseudomonas sp. LDC-5 |
9psed-q6ufw1 | Pseudomonas sp. HJ-2 mcl-pha synthase 2 | Pseudomonas sp. HJ-2 |
9psed-q6ufw2 | Pseudomonas sp. HJ-2 mcl-pha synthase 1 | Pseudomonas sp. HJ-2 |
9sphn-q2n9u1 | Erythrobacter litoralis HTCC2594 pha-synthase 1 poly(3-hydroxyalkanoate) polymerase 1 | Erythrobacter litoralis HTCC2594 |
burca-PHAC1 | Burkholderia caryophylli pha synthase 1 | Burkholderia caryophylli |
burca-PHAC2 | Burkholderia caryophylli pha synthase 2 | Burkholderia caryophylli |
burs3-q396v7 | Burkholderia sp. (strain 383) (Burkholderia cepacia (strain ATCC 1776 poly-beta-hydroxybutyrate polymerase | Burkholderia sp. |
comte-q5q137 | Comamonas testosteroni (Pseudomonas testosteroni) Pseudomonas sp. KBOS 0 P 3Y2 KBOS 03 KBOS 17 pha Aeromonas hydrophila synthase II | Comamonas testosteroni |
comte-q5q139 | Comamonas testosteroni (Pseudomonas testosteroni) Pseudomonas sp. 3Y2 KBOS 1 KBOS 03 KBOS 04 3Y2 pha synthase I | Comamonas testosteroni |
cuppj-q46zd1 | Cupriavidus pinatubonensis (strain JMP134 \/ LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) terminal | Ralstonia eutropha |
pse14-q48pi7 | Pseudomonas syringae pv. phaseolicola (strain 1448A \/ Race 6) pha-synthase 1 (EC 2.3.1.-) | Pseudomonas syringae pv. phaseolicola |
pse14-q48pi9 | Pseudomonas syringae, Pseudomonas savastanoi, poly(3-hydroxyalkanoate) polymerase (EC 2.3.1.-) | Pseudomonas syringae pv. phaseolicola |
pseae-PHAC1 | Pseudomonas aeruginosa pha-synthase1 (poly(3-hydroxyalkanoic acid) synthase 1) PA5056 | Pseudomonas aeruginosa |
pseae-PHAC2 | Pseudomonas aeruginosa pha-synthase2 (poly(3-hydroxyalkanoic acid) synthase 2) PA5058 phaC2 | Pseudomonas aeruginosa |
psech-PHAC1 | Pseudomonas chlororaphis (Pseudomonas aureofaciens) pha-synthase 1 | Pseudomonas chlororaphis |
psech-PHAC2 | Pseudomonas chlororaphis (Pseudomonas aureofaciens) pha-synthase 2 | Pseudomonas chlororaphis |
psef5-q4kjk5 | Pseudomonas fluorescens (strain Pf-5 \/ ATCC BAA-477) poly(3-hydroxyalkanoate) polymerase 1 | Pseudomonas fluorescens |
psef5-q4kjk7 | Pseudomonas fluorescens (strain Pf-5 \/ ATCC BAA-477) poly(3-hydroxyalkanoate) polymerase | Pseudomonas fluorescens |
psefl-q6xh00 | Pseudomonas fluorescens phac | Pseudomonas fluorescens |
pseme-PHAC1 | Pseudomonas mendocina, Pseudomonas nitroreducens, Pseudomonas pseudoalcaligenes, P. stutzeri (P. perfectomarina), P. oleovorans, P. sp., pha synthase 1 (type II pha polymerase) | Pseudomonas mendocina |
pseme-PHAC2 | Pseudomonas mendocina, Pseudomonas pseudoalcaligenes, Pseudomonas sp., Pseudomonas oleovorans, pha synthase 2 | Pseudomonas mendocina |
pseol-phaa | Pseudomonas oleovorans (pha synthase 1) (polyhydroxyalkanoic acid synthase 1) | Pseudomonas oleovorans |
pseol-phac | Pseudomonas oleovorans (pha synthase 2) (polyhydroxyalkanoic acid synthase 2) | Pseudomonas oleovorans |
psepf-q3kjb8 | Pseudomonas fluorescens (strain PfO-1) poly(r)-hydroxyalkanoic acid synthase, class II | Pseudomonas fluorescens |
psepf-q3kjc0 | Pseudomonas fluorescens (strain PfO-1) poly(r)-hydroxyalkanoic acid synthase, class II | Pseudomonas fluorescens |
psepu-PHAC1 | Pseudomonas putida (Arthrobacter siderocapsulatus), Pseudomonas sp., pha synthase 1 poly(3-hydroxyalkanoate) polymerase 1 | Pseudomonas putida |
psepu-PHAC2 | Pseudomonas putida (Arthrobacter siderocapsulatus) poly(3-hydroxyalkanoate) synthase 2 | Pseudomonas putida |
psere-PHAC1 | Pseudomonas resinovorans pha synthase 1 | Pseudomonas resinovorans |
psere-PHAC2 | Pseudomonas resinovorans pha synthase 2 | Pseudomonas resinovorans |
psesp-PHAC1 | Pseudomonas sp. 61-3 pha synthase 1 | Pseudomonas sp. 61-3 |
psesp-PHAC2 | Pseudomonas sp. 61-3 pha synthase 2 | Pseudomonas sp. 61-3 |
psest-PHAC2 | Pseudomonas stutzeri (Pseudomonas perfectomarina) pha synthase II | Pseudomonas stutzeri |
psesy-PHAC | Pseudomonas syringae (pv. tomato) poly(3-hydroxyalkanoate) polymerase | Pseudomonas syringae |
rhoco-PHAC | Rhodococcus corallinus\; Rhodococcus aetherivorans I24 pha synthase | Rhodococcus corallinus |
Substrate | Chemical Nomenclature | Proteins |
---|---|---|
3-hydroxybutyrate | bacce-PHAC alceu-phbc psefl-q6xh00 | |
3-hydroxydecanoic-acid | (3S)-3-hydroxydecanoic acid | psere-PHAC1 |
3HB-CoA | S-[2-[3-[[(2R)-4-[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-2-hydroxy-3,3-dimethylbutanoyl]amino]propanoylamino]ethyl] 3-hydroxybutanethioate | ralso-PHBC alceu-phbc chrvi-phbc bacce-PHAC psefl-q6xh00 |
P(3HB-co-3HHx) | alceu-phbc rhoru-PHBCRR rhoco-PHAC 9neis-e1apk1 | |
Polylactic-acid | 2-Hydroxypropanoic acid homopolymer | paeam-PLAA thefu-q6a0i3 rhopa-q6n9m9 alcbs-q0vlq1 9zzzz-A0A0G3FEJ8 9acto-f7ix06 crysp-Q874E9 9bact-a4uz14 9bact-a4uz10 psea2-s6bc01 thefu-q6a0i4 aspng-g9m5r3 psecl-LIP1 psecl-LIP2 psecl-LIP3 9bact-a4uz12 9zzzz-a0a0g3fj39 bacpu-q6rsn0 fusso-cutas |
Title : Polyhydroxyalkanoate synthase (PhaC): The key enzyme for biopolyester synthesis - Neoh_2022_Curr.Res.Biotechnol_4_87 |
Author(s) : Neoh SZ , Chek MF , Tan HT , Linares-Pasten JA , Nandakumar A , Hakoshima T , Sudesh K |
Ref : Current Research in Biotechnology , 4 :87 , 2022 |
Abstract : |
PubMedSearch : Neoh_2022_Curr.Res.Biotechnol_4_87 |
PubMedID: |
Title : Polyester synthases: natural catalysts for plastics - Rehm_2003_Biochem.J_376_15 |
Author(s) : Rehm BH |
Ref : Biochemical Journal , 376 :15 , 2003 |
Abstract : |
PubMedSearch : Rehm_2003_Biochem.J_376_15 |
PubMedID: 12954080 |
Title : PhaC and PhaR are required for polyhydroxyalkanoic acid synthase activity in Bacillus megaterium - McCool_2001_J.Bacteriol_183_4235 |
Author(s) : McCool GJ , Cannon MC |
Ref : Journal of Bacteriology , 183 :4235 , 2001 |
Abstract : |
PubMedSearch : McCool_2001_J.Bacteriol_183_4235 |
PubMedID: 11418564 |
Gene_locus related to this paper: bacme-PHAC |
Title : Cloning, molecular analysis, and expression of the polyhydroxyalkanoic acid synthase (phaC) gene from Chromobacterium violaceum - Kolibachuk_1999_Appl.Environ.Microbiol_65_3561 |
Author(s) : Kolibachuk D , Miller A , Dennis D |
Ref : Applied Environmental Microbiology , 65 :3561 , 1999 |
Abstract : |
PubMedSearch : Kolibachuk_1999_Appl.Environ.Microbiol_65_3561 |
PubMedID: 10427049 |
Gene_locus related to this paper: chrvi-PHAC , vibpa-PHAC |