thefu-q6a0i3
Thermobifida cellulosilytica; Thermomonospora fusca (and strain YX) BTA-hydrolase 2 Cutinase AAZ54921 Tfu_0882 TfCut2 Thc_Cut2 PET hydrolase
Comment
Many mutants of TfCut2 tested for imroved PET hydrolysis. Other strains: Thermobifida cellulosilytica\; Thermomonospora fusca (and strain YX)\; Burkholderia cepacia (Pseudomonas cepacia)
Relationship
Family : Polyesterase-lipase-cutinase
Block : L
Position in NCBI Life Tree : Thermomonospora fusca
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Terrabacteria group N E > Actinobacteria [phylum] N E > Actinobacteria [class] N E > Streptosporangiales N E > Nocardiopsaceae N E > Thermobifida N E > Thermobifida fusca N EMolecular evidence
No mutation
5LUJ - Structural characterization of Thc_Cut2 cutinase from Thermobifida cellulosilytica
5LUK - Structural characterization of Thc_Cut2DM cutinase from Thermobifida cellulosilytica
5LUL - Structural characterization of triple mutant of cutinase2 from Thermobifida cellulosilytica
7QJS - Thermobifida fusca 705 AAZ54920 hydrolase
No kinetic
No disease
Database
Sequence
Peptide
MAVMTPRRER SSLLSRALRF TAAAATALVT AVSLAAPAHA ANPYERGPNP TDALLEARSG PFSVSEERAS RFGADGFGGG TIYYPRENNT YGAVAISPGY TGTQASVAWL GKRIASHGFV VITIDTNTTL DQPDSRARQL NAALDYMIND ASSAVRSRID SSRLAVMGHS MGGGGSLRLA SQRPDLKAAI PLTPWHLNKN WSSVRVPTLI IGADLDTIAP VLTHARPFYN SLPTSISKAY LELDGATHFA PNIPNKIIGK YSVAWLKRFV DNDTRYTQFL CPGPRDGLFG EVEEYRSTCP F
References (25)
| Title : Selective Hydrolysis by Engineered Cutinases: Characterization of Aliphatic-Aromatic Homo and Co-Polyesters by LC and LC-MS methods - Abdelraheem_2025_Chemistry__e202403879 |
| Author(s) : Abdelraheem E , Tseliou V , Desport J , Serizawa M , Schurmann M , Buijsen P , Peters R , Gargano AFG , Mutti FG |
| Ref : Chemistry , :e202403879 , 2025 |
| Abstract : |
| PubMedSearch : Abdelraheem_2025_Chemistry__e202403879 |
| PubMedID: 39936453 |
| Gene_locus related to this paper: 9bact-g9by57 , thefu-q6a0i3 |
| Title : Analysis of the product-spectrum during the biocatalytic hydrolysis of PEF (poly(ethylene furanoate)) with various esterases - Heinks_2025_RSC.Sustain__ |
| Author(s) : Heinks T , Hofmann K , Zimmermann L , Gamm I , Lieb A , Blach L , Wei R , Bornscheuer U.T , Thiele J , Hamel C , von Langermann J |
| Ref : RSC Sustainability , : , 2025 |
| Abstract : |
| PubMedSearch : Heinks_2025_RSC.Sustain__ |
| PubMedID: |
| Gene_locus related to this paper: deiml-e8u721 , 9bact-c3ryl0 , 9firm-PHL7 , 9bact-a0a2h5z9r5 , idesa-peth , 9bact-g9by57 , morsp-lip1 , thefu-q6a0i3 , sacvd-c7mve8 |
| Title : Enabling high-throughput enzyme discovery and engineering with a low-cost, robot-assisted pipeline - Norton-Baker_2024_Sci.Rep_14_14449 |
| Author(s) : Norton-Baker B , Denton MCR , Murphy NP , Fram B , Lim S , Erickson E , Gauthier NP , Beckham GT |
| Ref : Sci Rep , 14 :14449 , 2024 |
| Abstract : |
| PubMedSearch : Norton-Baker_2024_Sci.Rep_14_14449 |
| PubMedID: 38914665 |
| Gene_locus related to this paper: 9actn-a0a1m7II12 , 9firm-PHL7 , 9bact-a0a2h5z9r5 , idesa-peth , 9bact-g9by57 , 9pseu-a0a1i6nu60 , thefu-q6a0i3 , sacvd-c7mve8 |
| Title : Enhancement of PET biodegradation by anchor peptide-cutinase fusion protein - Liu_2022_Enzyme.Microb.Technol_156_110004 |
| Author(s) : Liu Z , Zhang Y , Wu J |
| Ref : Enzyme Microb Technol , 156 :110004 , 2022 |
| Abstract : |
| PubMedSearch : Liu_2022_Enzyme.Microb.Technol_156_110004 |
| PubMedID: 35217214 |
| Gene_locus related to this paper: thefu-q6a0i3 |
| Title : Directional-path modification strategy enhances PET hydrolase catalysis of plastic degradation - Chen_2022_J.Hazard.Mater_433_128816 |
| Author(s) : Chen XQ , Guo ZY , Wang L , Yan ZF , Jin CX , Huang QS , Kong DM , Rao DM , Wu J |
| Ref : J Hazard Mater , 433 :128816 , 2022 |
| Abstract : |
| PubMedSearch : Chen_2022_J.Hazard.Mater_433_128816 |
| PubMedID: 35390614 |
| Gene_locus related to this paper: thefu-q6a0i3 |
| Title : Computational design of a cutinase for plastic biodegradation by mining molecular dynamics simulations trajectories - Li_2022_Comput.Struct.Biotechnol.J_20_459 |
| Author(s) : Li Q , Zheng Y , Su T , Wang Q , Liang Q , Zhang Z , Qi Q , Tian J |
| Ref : Comput Struct Biotechnol J , 20 :459 , 2022 |
| Abstract : |
| PubMedSearch : Li_2022_Comput.Struct.Biotechnol.J_20_459 |
| PubMedID: 35070168 |
| Gene_locus related to this paper: thefu-q6a0i3 |
| Title : Cation-pi and hydrophobic interaction controlled PET recognition in double mutated cutinase - identification of a novel binding subsite for better catalytic activity - James_2022_RSC.Adv_12_20563 |
| Author(s) : James A , De S |
| Ref : RSC Adv , 12 :20563 , 2022 |
| Abstract : |
| PubMedSearch : James_2022_RSC.Adv_12_20563 |
| PubMedID: 35919142 |
| Gene_locus related to this paper: thefu-q6a0i3 |
| Title : Unveiling the Enzymatic Degradation Process of Biobased Thiophene Polyesters - Bertolini_2021_Front.Chem_9_771612 |
| Author(s) : Bertolini FA , Soccio M , Weinberger S , Guidotti G , Gazzano M , Guebitz GM , Lotti N , Pellis A |
| Ref : Front Chem , 9 :771612 , 2021 |
| Abstract : |
| PubMedSearch : Bertolini_2021_Front.Chem_9_771612 |
| PubMedID: 34869219 |
| Gene_locus related to this paper: thefu-q6a0i4 , thefu-q6a0i3 |
| Title : Structure-function analysis of two closely related cutinases from Thermobifida cellulosilytica - Baath_2021_Biotechnol.Bioeng__ |
| Author(s) : Baath JA , Novy V , Carneiro LV , Guebitz GM , Olsson L , Westh P , Ribitsch D |
| Ref : Biotechnol Bioeng , : , 2021 |
| Abstract : |
| PubMedSearch : Baath_2021_Biotechnol.Bioeng__ |
| PubMedID: 34755331 |
| Gene_locus related to this paper: thefu-q6a0i4 , thefu-q6a0i3 |
| Title : Enzymatic degradation of polyethylene terephthalate nanoplastics analyzed in real time by isothermal titration calorimetry - Vogel_2021_Sci.Total.Environ_773_145111 |
| Author(s) : Vogel K , Wei R , Pfaff L , Breite D , Al-Fathi H , Ortmann C , Estrela-Lopis I , Venus T , Schulze A , Harms H , Bornscheuer UT , Maskow T |
| Ref : Sci Total Environ , 773 :145111 , 2021 |
| Abstract : |
| PubMedSearch : Vogel_2021_Sci.Total.Environ_773_145111 |
| PubMedID: 33940717 |
| Gene_locus related to this paper: thefu-q6a0i3 |
| Title : Comparative biochemistry of four polyester (PET) hydrolases - Baath_2020_Chembiochem_22_1627 |
| Author(s) : Baath JA , Borch K , Jensen K , Brask J , Westh P |
| Ref : Chembiochem , 22 :1627 , 2020 |
| Abstract : |
| PubMedSearch : Baath_2020_Chembiochem_22_1627 |
| PubMedID: 33351214 |
| Gene_locus related to this paper: idesa-peth , humin-cut , bacsu-pnbae , thefu-q6a0i4 , thefu-q6a0i3 |
| Title : Efficient Degradation of Poly(ethylene terephthalate) with Thermobifida fusca Cutinase Exhibiting Improved Catalytic Activity Generated using Mutagenesis and Additive-based Approaches - Furukawa_2019_Sci.Rep_9_16038 |
| Author(s) : Furukawa M , Kawakami N , Tomizawa A , Miyamoto K |
| Ref : Sci Rep , 9 :16038 , 2019 |
| Abstract : |
| PubMedSearch : Furukawa_2019_Sci.Rep_9_16038 |
| PubMedID: 31690819 |
| Gene_locus related to this paper: thefu-q6a0i3 |
| Title : Contribution of disulfide bond to the stability of Thermobifida fusca cutinase - Chen_2019_Food.Biosci_27_6 |
| Author(s) : Chen S , Wu Y , Su L , Wu J |
| Ref : Food Biosci , 27 :6 , 2019 |
| Abstract : |
| PubMedSearch : Chen_2019_Food.Biosci_27_6 |
| PubMedID: |
| Gene_locus related to this paper: thefu-q6a0i3 |
| Title : Small cause, large effect: Structural characterization of cutinases from Thermobifida cellulosilytica - Ribitsch_2017_Biotechnol.Bioeng_114_2481 |
| Author(s) : Ribitsch D , Hromic A , Zitzenbacher S , Zartl B , Gamerith C , Pellis A , Jungbauer A , Lyskowski A , Steinkellner G , Gruber K , Tscheliessnig R , Herrero Acero E , Guebitz GM |
| Ref : Biotechnol Bioeng , 114 :2481 , 2017 |
| Abstract : |
| PubMedSearch : Ribitsch_2017_Biotechnol.Bioeng_114_2481 |
| PubMedID: 28671263 |
| Gene_locus related to this paper: thefu-q6a0i4 , thefu-q6a0i3 |
| Title : Degradation of Polyester Polyurethane by Bacterial Polyester Hydrolases - Schmidt_2017_Polymers.(Basel)_9_65 |
| Author(s) : Schmidt J , Wei R , Oeser T , Dedavid e Silva LA , Breite D , Schulze A , Zimmermann W |
| Ref : Polymers (Basel) , 9 :65 , 2017 |
| Abstract : |
| PubMedSearch : Schmidt_2017_Polymers.(Basel)_9_65 |
| PubMedID: 30970745 |
| Gene_locus related to this paper: thecd-d1a9g5 , thecd-d1a2h1 , 9bact-g9by57 , thefu-q6a0i4 , thefu-q6a0i3 |
| Title : Turbidimetric analysis of the enzymatic hydrolysis of polyethylene terephthalate nanoparticles - Wei_2014_J.Mol.Catal.B.Enzym_103_72 |
| Author(s) : Wei R , Oeser O , Barth M , Weigl N , Luebs A , Schulz-Siegmund M , Hacker MC , Zimmermann W |
| Ref : J Mol Catal B Enzym , 103 :72 , 2014 |
| Abstract : |
| PubMedSearch : Wei_2014_J.Mol.Catal.B.Enzym_103_72 |
| PubMedID: |
| Gene_locus related to this paper: thefu-q6a0i3 |
| Title : Novel cutinase from Pseudomonas cepacia NRRL B 2320: Purification, characterization and identification of cutinase encoding genes - Dutta_2013_J.Gen.Appl.Microbiol_59_171 |
| Author(s) : Dutta K , Krishnamoorthy H , Venkata Dasu V |
| Ref : J Gen Appl Microbiol , 59 :171 , 2013 |
| Abstract : |
| PubMedSearch : Dutta_2013_J.Gen.Appl.Microbiol_59_171 |
| PubMedID: 23863287 |
| Gene_locus related to this paper: thefu-q6a0i4 , thefu-q6a0i3 |
| Title : Enzymatic Surface Hydrolysis of PET: Effect of Structural Diversity on Kinetic Properties of Cutinases from Thermobifida. - Herrero-Acero_2011_Macromolecules_44_4632 |
| Author(s) : Herrero Acero E , Ribitsch D , Steinkellner G , Gruber K , Greimel K , Eiteljoerg I , Trotscha E , Wei R , Zimmermann W , Zinn M , Cavaco-Paulo A , Freddi G , Schwab H , Guebitz G |
| Ref : Macromolecules , 44 :4632 , 2011 |
| Abstract : |
| PubMedSearch : Herrero-Acero_2011_Macromolecules_44_4632 |
| PubMedID: |
| Gene_locus related to this paper: bacsu-pnbae , thefu-q6a0i4 , thefu-q6a0i3 |
| Title : Biochemical characterization of the cutinases from Thermobifida fusca - Chen_2010_J.Mol.Catal.B.Enzym_63_121 |
| Author(s) : Chen S , Su L , Billig S , Zimmermann W , Chen J , Wu J |
| Ref : J Mol Catal B Enzym , 63 :121 , 2010 |
| Abstract : |
| PubMedSearch : Chen_2010_J.Mol.Catal.B.Enzym_63_121 |
| PubMedID: |
| Gene_locus related to this paper: thefu-q6a0i4 , thefu-q6a0i3 |
| Title : Recombinant expression of BTA hydrolase in Streptomyces rimosus and catalytic analysis on polyesters by surface plasmon resonance - Sinsereekul_2010_Appl.Microbiol.Biotechnol_86_1775 |
| Author(s) : Sinsereekul N , Wangkam T , Thamchaipenet A , Srikhirin T , Eurwilaichitr L , Champreda V |
| Ref : Applied Microbiology & Biotechnology , 86 :1775 , 2010 |
| Abstract : |
| PubMedSearch : Sinsereekul_2010_Appl.Microbiol.Biotechnol_86_1775 |
| PubMedID: 20174792 |
| Gene_locus related to this paper: thefu-q6a0i3 |
| Title : Identification and characterization of bacterial cutinase - Chen_2008_J.Biol.Chem_283_25854 |
| Author(s) : Chen S , Tong X , Woodard RW , Du G , Wu J , Chen J |
| Ref : Journal of Biological Chemistry , 283 :25854 , 2008 |
| Abstract : |
| PubMedSearch : Chen_2008_J.Biol.Chem_283_25854 |
| PubMedID: 18658138 |
| Gene_locus related to this paper: thefu-q6a0i4 , thefu-q6a0i3 |
| Title : Genome sequence and analysis of the soil cellulolytic actinomycete Thermobifida fusca YX - Lykidis_2007_J.Bacteriol_189_2477 |
| Author(s) : Lykidis A , Mavromatis K , Ivanova N , Anderson I , Land M , DiBartolo G , Martinez M , Lapidus A , Lucas S , Copeland A , Richardson P , Wilson DB , Kyrpides N |
| Ref : Journal of Bacteriology , 189 :2477 , 2007 |
| Abstract : |
| PubMedSearch : Lykidis_2007_J.Bacteriol_189_2477 |
| PubMedID: 17209016 |
| Gene_locus related to this paper: thefu-q6a0i3 , thefy-q47l17 , thefy-q47p38 , thefy-q47pm5 |
| Title : Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca in Escherichia coli - Dresler_2006_Bioprocess.Biosyst.Eng_29_169 |
| Author(s) : Dresler K , van den Heuvel J , Muller RJ , Deckwer WD |
| Ref : Bioprocess Biosyst Eng , 29 :169 , 2006 |
| Abstract : |
| PubMedSearch : Dresler_2006_Bioprocess.Biosyst.Eng_29_169 |
| PubMedID: 16770590 |
| Gene_locus related to this paper: thefu-q6a0i3 |
| Title : Enzymatic degradation of poly(ethylene terephthalate):rapid hydrolyse using a hydrolase from T.fusca - Muller_2005_Macromol.Rapid.Commun_26_1400 |
| Author(s) : R.J. Muller, H. Schrader, J. Profe, K. Dresler, W.D. Deckwer , Muller RJ , Schrader H , Profe J , Dresler K , Deckwer WD |
| Ref : Macromol Rapid Commun , 26 :1400 , 2005 |
| Abstract : |
| PubMedSearch : Muller_2005_Macromol.Rapid.Commun_26_1400 |
| PubMedID: |
| Gene_locus related to this paper: thefu-1831 , thefu-q6a0i3 |