9firm-PHL7
Thermoanaerobacterales bacterium Polyester Hydrolase Leipzig 7 (PHL-7) PHL7 PES-H1 PesH1
Comment
PHL7 PES-H1 PESH-2 are very similar proteins PHL7 and PES-H1 are identical and PES-H2 differs in only four residues (A\/E1, L\/F209, D\/N232, and S\/A254), only the L\/F209 is close to the catalytic triad. These proteins are not separated here and considered as mutants of the same entry. See also PHL7-Jemez and FlashPETase || PHL7-WT (GenBank: PQ223703), PHL7-Jemez (GenBank: PQ223704), PHL7-Santa Fe (GenBank: PQ223705), PHL7-Taos (GenBank: PQ223706), PHL7-Tusas (GenBank: PQ223707), PHL7-L93F\/Q95Y (GenBank: PQ223708), PHL7-A1 (GenBank: PQ223709), PHL7-A2 (GenBank: PQ223710), PHL7-A3 (GenBank: PQ223711), PHL7-A4 (GenBank: PQ223712), PHL7-A5 (GenBank: PQ223713), PHL7-A6 (GenBank: PQ223714), PHL7-B1 (GenBank: PQ223715), PHL7-B2 (GenBank: PQ223716), PHL7-B3 (GenBank: PQ223717), PHL7-C1 (GenBank: PQ223718), PHL7-C2 (GenBank: PQ223719), PHL7-C3 (GenBank: PQ223720), PHL7-C4 (GenBank: PQ223721), PHL7-C5 (GenBank: PQ223722), and PHL7-C6 (GenBank: PQ223723). || . Mutations: R1M1 (Q104L R111T T145P E148K G155P T167V L235T F248P)\; R1M2 (Q104L R111T T145P E148K G155P T167V L176N T219I L235T F248P)\; R1M3 (G66T Q104L R111T T145P E148K G155P T167V L176N S212T T219I L235T F248P)\; R1M4 (G66T E68S Q104L H109Y R111T T145P E148K G155P T167V L176N S186A S212T T219I L235T F248P)\; R1M5(V19A G66T E68S I90N Q104L H109Y R111T T145P E148K G155P T167V V172I L176N S186A S212T T219I L235T F248P)\; R1M6 (V19A D36S G66T E68S I90N Q104L H109Y R111T V115T N118D T145P E148K G155P T167V V171I V172I L176N S186A S212T D216N T219I L235T F248P)\; R1M7 (V19A D36S G66T E68S I90N Q104L H109Y R111T N113D V115T N118D T145P E148K G155P T167V V171I V172I L176N S186A D196S S212T D216N T219I D233N L235T F248P)\; R1M8 (S14A V19P D36R G66T E68S I77L I90N L93Y Q104L H109Y R111T N113D V115T N118D S139A T145P E148K G155P T167V V171I V172I L176N S184Q S186A D196S S212T D216N T219I S223A L235T F248P)\; R1M9 (E6Q S14A V19P Q34D D36R F63Y T64L G66T E68S I77L I90N T91S L93Y Q104L H109Y R111T N113D V115T N118D S139A T145P E148K G155P N161D T167V V171I V172I L176N S184Q S186A D196S D198P S208D S212A N213T D216N T219I S223V L235T F248P)\; R2M1 (E68S Q104L H109Y R111T N113D T145P E148K G155P T167V L176N S186A D196S T219I L235T F248P)\; R2M2 (E6Q Q34D D36S E68S T91S Q104L H109Y R111T N113D V115T N118D T145P E148K G155P N161D T167V V171I S186A D196S D198P S208D D216N L235T F248P)\; R4M6(=R2M2-P155G+A186S+D208S+L104Q+Q175E+L210T)\; R4M10 (=R4M6+L93F+Q95Y)\; R4M12 (=R2M2-P155G+A186S+D208S+L104Q+Q175E+L210T+K148E+D233K)
Relationship
Family : Polyesterase-lipase-cutinase
Block : L
Position in NCBI Life Tree : Thermoanaerobacterales bacterium
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Terrabacteria group N E > Firmicutes N E > Clostridia N E > Thermoanaerobacterales N E > unclassified Thermoanaerobacterales N E > Thermoanaerobacterales bacterium N EMolecular evidence
Database
Sequence
Peptide
MRHMSTSIAR VRTRLGALLA GVIVAGSTVI GAAPAGAQEN PYERGPDPTE SSIEAVRGPF AVAQTTVSRL QADGFGGGTI YYPTDTSQGT FGAVAISPGF TAGQESIAWL GPRIASQGFV VITIDTITRL DQPDSRGRQL QAALDHLRTN SVVRNRIDPN RMAVMGHSMG GGGALSAAAN NTSLEAAIPL QGWHTRKNWS SVRTPTLVVG AQLDTIAPVS SHSEAFYNSL PSDLDKAYME LRGASHLVSN TPDTTTAKYS IAWLKRFVDD DLRYEQFLCP APDDFAISEY RSTCPHSGSG GGTTPPPEDD CPWWAWWC
References (20)
| Title : Computational engineering of the polyester hydrolase PHL7 for efficient poly(ethylene terephthalate) degradation in biocatalytic recycling processes - Blazquez-Sanchez_2026_Nat.Commun_17_4370 |
| Author(s) : Blazquez-Sanchez P , Gunkel J , Useini A , Zlobin A , Zakary JD , Scholer A , Graefe N , Engelberger F , Cantanhede F , Frank R , Zhao Z , Zarei A , Butenschon E , Matysik J , Zimmermann W , Strater N , Sonnendecker C , Kunze G |
| Ref : Nat Commun , 17 :4370 , 2026 |
| Abstract : |
| PubMedSearch : Blazquez-Sanchez_2026_Nat.Commun_17_4370 |
| PubMedID: 42140896 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Rapid Identification of Superior Endogenous Signal Peptides for Heterologous Protein Secretion by Corynebacterium glutamicum Through Modular Cloning and Automation - Matamouros_2026_Microb.Biotechnol_19_e70299 |
| Author(s) : Matamouros S , Tenhaef J , Bida A , Noack S , Bott M |
| Ref : Microb Biotechnol , 19 :e70299 , 2026 |
| Abstract : |
| PubMedSearch : Matamouros_2026_Microb.Biotechnol_19_e70299 |
| PubMedID: 41543490 |
| Gene_locus related to this paper: 9firm-PHL7 , 9gamm-Hoce , fusso-cutas |
| Title : Two-step biocatalytic conversion of post-consumer polyethylene terephthalate into value-added products facilitated by genetic and bioprocess engineering - Welsing_2025_Bioresour.Technol_417_131837 |
| Author(s) : Welsing G , Wolter B , Kleinert GEK , Gottsch F , Besenmatter W , Xue R , Mauri A , Steffens D , Kobbing S , Dong W , Jiang M , Bornscheuer UT , Wei R , Tiso T , Blank LM |
| Ref : Bioresour Technol , 417 :131837 , 2025 |
| Abstract : |
| PubMedSearch : Welsing_2025_Bioresour.Technol_417_131837 |
| PubMedID: 39557102 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Cutinases for Biocatalytic Polycarbonate Hydrolysis: Empowering Circular Economy - Schell_2025_ChemSusChem__e202500944 |
| Author(s) : Schell K , Gobbels S , Pastor L , Donets S , Knopp D , Bamberg O , Jager G , Schwaneberg U , Reisky L |
| Ref : ChemSusChem , :e202500944 , 2025 |
| Abstract : |
| PubMedSearch : Schell_2025_ChemSusChem__e202500944 |
| PubMedID: 40460075 |
| Gene_locus related to this paper: 9firm-PHL7 , 9bact-a0a2h5z9r5 , 9bact-g9by57 , thefu-q6a0i4 , sacvd-c7mve8 |
| Title : Trade-Offs between Stability and Activity of Glycosylated and Non-Glycosylated Polyester Hydrolases PHL7 and PHL7mut3 - Fohler_2025_Acs.Es.T.Eng__ |
| Author(s) : Fohler L , Faschingeder F , Leibetseder L , Zhao Z , Useini A , Strater N , Sonnendecker C , Ewing TA , Moers APHA , Werten MWT , van Vliet DM , Julsing MK , Zimmermann W , Striedner G |
| Ref : Acs Es T Eng , : , 2025 |
| Abstract : |
| PubMedSearch : Fohler_2025_Acs.Es.T.Eng__ |
| PubMedID: |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Engineering PHL7 for improved poly(ethylene terephthalate) depolymerization via rational design and directed evolution - Groseclose_2025_Chem.Catal_5_101399 |
| Author(s) : Groseclose TM , Kober E , Clark M , Moore B , Jha RK , Taylor ZK , Lujan LA , Beckham GT , Pickford AR , Dale T , Nguyen HB |
| Ref : Chem Catal , 5 :101399 , 2025 |
| Abstract : |
| PubMedSearch : Groseclose_2025_Chem.Catal_5_101399 |
| PubMedID: 40862219 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Energy-guided accumulated mutation strategy achieves a highly efficient polyethylene terephthalate-degrading enzyme - Wang_2025_Biochem.Eng.J_219_109708 |
| Author(s) : Wang Z , Zhang J , You S , Su R , Qi W |
| Ref : Biochem Eng J , 219 :109708 , 2025 |
| Abstract : |
| PubMedSearch : Wang_2025_Biochem.Eng.J_219_109708 |
| PubMedID: |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Analysis of the product-spectrum during the biocatalytic hydrolysis of PEF (poly(ethylene furanoate)) with various esterases - Heinks_2025_RSC.Sustain__ |
| Author(s) : Heinks T , Hofmann K , Zimmermann L , Gamm I , Lieb A , Blach L , Wei R , Bornscheuer U.T , Thiele J , Hamel C , von Langermann J |
| Ref : RSC Sustainability , : , 2025 |
| Abstract : |
| PubMedSearch : Heinks_2025_RSC.Sustain__ |
| PubMedID: |
| Gene_locus related to this paper: deiml-e8u721 , 9bact-c3ryl0 , 9firm-PHL7 , 9bact-a0a2h5z9r5 , idesa-peth , 9bact-g9by57 , morsp-lip1 , thefu-q6a0i3 , sacvd-c7mve8 |
| Title : Unveiling PET Hydrolase Surface Dynamics through Fluorescence Microscopy - Rennison_2024_Chembiochem__e202300661 |
| Author(s) : Rennison AP , Nousi A , Westh P , Marie R , Moller MS |
| Ref : Chembiochem , :e202300661 , 2024 |
| Abstract : |
| PubMedSearch : Rennison_2024_Chembiochem__e202300661 |
| PubMedID: 38224131 |
| Gene_locus related to this paper: 9firm-PHL7 , 9bact-g9by57 |
| Title : Manufacturing of the highly active thermophile PETases PHL7 and PHL7mut3 using Escherichia coli - Fohler_2024_Microb.Cell.Fact_23_272 |
| Author(s) : Fohler L , Leibetseder L , Cserjan-Puschmann M , Striedner G |
| Ref : Microb Cell Fact , 23 :272 , 2024 |
| Abstract : |
| PubMedSearch : Fohler_2024_Microb.Cell.Fact_23_272 |
| PubMedID: 39390547 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : From Bulk to Binding: Decoding the Entry of PET into Hydrolase Binding Pockets - Jackering_2024_JACS.Au_4_4000 |
| Author(s) : Jackering A , Gottsch F , Schaffler M , Doerr M , Bornscheuer UT , Wei R , Strodel B |
| Ref : JACS Au , 4 :4000 , 2024 |
| Abstract : |
| PubMedSearch : Jackering_2024_JACS.Au_4_4000 |
| PubMedID: 39483243 |
| Gene_locus related to this paper: 9firm-PHL7 , 9bact-g9by57 |
| Title : One-Pot Depolymerization of Mixed Plastics Using a Dual Enzyme System - Wei_2024_ChemSusChem__e202402416 |
| Author(s) : Wei R , Branson Y , Liu J , Schmidt L , Griebel J , Prager A , Stieler L , Breite D , Bayer T , Besenmatter W , Dong W , Garscha U , Bornscheuer UT |
| Ref : ChemSusChem , :e202402416 , 2024 |
| Abstract : |
| PubMedSearch : Wei_2024_ChemSusChem__e202402416 |
| PubMedID: 39714869 |
| Gene_locus related to this paper: 9firm-PHL7 , pyrca-PCEST |
| Title : Activity of an anaerobic Thermoanaerobacterales hydrolase on aliphatic and aromatic polyesters - Siracusa_2024_Front.Bioeng.Biotechnol_12_1520680 |
| Author(s) : Siracusa C , Fohler L , Leibetseder L , Striedner G , Oostenbrink C , Quartinello F , Guebitz GM , Ribitsch D |
| Ref : Front Bioeng Biotechnol , 12 :1520680 , 2024 |
| Abstract : |
| PubMedSearch : Siracusa_2024_Front.Bioeng.Biotechnol_12_1520680 |
| PubMedID: 39898275 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Enabling high-throughput enzyme discovery and engineering with a low-cost, robot-assisted pipeline - Norton-Baker_2024_Sci.Rep_14_14449 |
| Author(s) : Norton-Baker B , Denton MCR , Murphy NP , Fram B , Lim S , Erickson E , Gauthier NP , Beckham GT |
| Ref : Sci Rep , 14 :14449 , 2024 |
| Abstract : |
| PubMedSearch : Norton-Baker_2024_Sci.Rep_14_14449 |
| PubMedID: 38914665 |
| Gene_locus related to this paper: 9actn-a0a1m7II12 , 9firm-PHL7 , 9bact-a0a2h5z9r5 , idesa-peth , 9bact-g9by57 , 9pseu-a0a1i6nu60 , thefu-q6a0i3 , sacvd-c7mve8 |
| Title : Structure and function of the metagenomic plastic-degrading polyester hydrolase PHL7 bound to its product - Richter_2023_Nat.Commun_14_1905 |
| Author(s) : Richter PK , Blazquez-Sanchez P , Zhao Z , Engelberger F , Wiebeler C , Kunze G , Frank R , Krinke D , Frezzotti E , Lihanova Y , Falkenstein P , Matysik J , Zimmermann W , Strater N , Sonnendecker C |
| Ref : Nat Commun , 14 :1905 , 2023 |
| Abstract : |
| PubMedSearch : Richter_2023_Nat.Commun_14_1905 |
| PubMedID: 37019924 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Assessment of Four Engineered PET Degrading Enzymes Considering Large-Scale Industrial Applications - Arnal_2023_ACS.Catal_13_13156 |
| Author(s) : Arnal G , Anglade J , Gavalda S , Tournier V , Chabot N , Bornscheuer UT , Weber G , Marty A |
| Ref : ACS Catal , 13 :13156 , 2023 |
| Abstract : |
| PubMedSearch : Arnal_2023_ACS.Catal_13_13156 |
| PubMedID: 37881793 |
| Gene_locus related to this paper: 9firm-PHL7 , idesa-peth , 9bact-g9by57 |
| Title : Low Carbon Footprint Recycling of Post-Consumer PET Plastic with a Metagenomic Polyester Hydrolase - Sonnendecker_2022_ChemSusChem_15_e202101062 |
| Author(s) : Sonnendecker C , Oeser J , Richter PK , Hille P , Zhao Z , Fischer C , Lippold H , Blazquez-Sanchez P , Engelberger F , Ramirez-Sarmiento CA , Oeser T , Lihanova Y , Frank R , Jahnke HG , Billig S , Abel B , Strater N , Matysik J , Zimmermann W |
| Ref : ChemSusChem , 15 :e202101062 , 2022 |
| Abstract : |
| PubMedSearch : Sonnendecker_2022_ChemSusChem_15_e202101062 |
| PubMedID: 34129279 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Real-Time Noninvasive Analysis of Biocatalytic PET Degradation - Frank_2022_ACS.Catal_12_25 |
| Author(s) : Frank R , Krinke D , Sonnendecker C , Zimmermann W , Jahnke HG |
| Ref : ACS Catal , 12 :25 , 2022 |
| Abstract : |
| PubMedSearch : Frank_2022_ACS.Catal_12_25 |
| PubMedID: |
| Gene_locus related to this paper: 9firm-PHL7 , 9bact-g9by57 |
| Title : Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase - Pfaff_2022_ACS.Catal_12_9790 |
| Author(s) : Pfaff L , Gao J , Li Z , Jackering A , Weber G , Mican J , Chen Y , Dong W , Han X , Feiler CG , Ao YF , Badenhorst CPS , Bednar D , Palm GJ , Lammers M , Damborsky J , Strodel B , Liu W , Bornscheuer UT , Wei R |
| Ref : ACS Catal , 12 :9790 , 2022 |
| Abstract : |
| PubMedSearch : Pfaff_2022_ACS.Catal_12_9790 |
| PubMedID: 35966606 |
| Gene_locus related to this paper: 9firm-PHL7 |