9firm-PHL7
Thermoanaerobacterales bacterium Polyester Hydrolase Leipzig 7 (PHL-7) PHL7 PES-H1
Comment
PHL7 PES-H1 PESH-2 are very similar proteins PHL7 and PES-H1 are identical and PES-H2 differs in only four residues (A\/E1, L\/F209, D\/N232, and S\/A254), only the L\/F209 is close to the catalytic triad. These proteins are not separated here and considered as mutants of the same entry.
Relationship
Family : Polyesterase-lipase-cutinase
Block : L
Position in NCBI Life Tree : Thermoanaerobacterales bacterium
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Terrabacteria group N E > Firmicutes N E > Clostridia N E > Thermoanaerobacterales N E > unclassified Thermoanaerobacterales N E > Thermoanaerobacterales bacterium N EMolecular evidence
No mutation
7CUV - Crystal structure of a novel alpha\/beta hydrolase in apo form PHL7 PES-H1
7E30 - Crystal structure of a novel alpha\/beta hydrolase in apo form PHL7 PES-H1 in complex with citrate
7E31 - Crystal structure of a novel alpha\/beta hydrolase mutant form PHL7 PES-H2 in complex with PEG6000
7NEI - Novel Polyester Hydrolase Leipzig 7 (PHL-7) PES-H1
7W66 - Crystal structure of a novel alpha\/beta hydrolase mutant form PHL7 PES-H2 in complex with PEG6000
7W69 - Crystal structure of a PSH1 mutant in complex with EDO PHL7 PES-H2
7W6C - Crystal structure of a novel alpha\/beta hydrolase PHL7 PES-H1 in complex with MHETA J1K
7W6O - Crystal structure of a novel alpha\/beta hydrolase PHL7 PES-H1 in complex with MHETA J1K
7W6Q - Crystal structure of a novel alpha\/beta hydrolase PHL7 PES-H1 in complex with MHETA J1K
8BRA - Polyester Hydrolase Leipzig 7 (PHL7) bound to terephthalic acid (TPA) and Mg2+
8BRB - Polyester Hydrolase Leipzig 7 (PHL7) bound to terephthalic acid (TPA)
9QBN - Crystal structure of glycosylated polyester hydrolase Leipzig 7 (PHL7) mut3 variant from Pichia (P_PHL7mut3)
9QDE - Crystal structure of non-glycosylated polyester hydrolase Leipzig 7 (PHL7) mut3 variant from Pichia (P_PHL7mut3_ng)
9QDV - Crystal structure of polyester hydrolase Leipzig 7 (PHL7) mut3 variant from E coli (E_PHL7mut3)
9QNM - Polyester Hydrolase Leipzig 7 (PHL7) variant R2M2
9QT8 - Polyester Hydrolase Leipzig 7 (PHL7) variant R2M2-P155G
9QV8 - Polyester Hydrolase Leipzig 7 (PHL7) variant R2M2-A186S
9QVA - Polyester Hydrolase Leipzig 7 (PHL7) variant R2M2-P155G-A186S
9QYA - Polyester Hydrolase Leipzig 7 (PHL7) variant R4M6
9QYB - Polyester Hydrolase Leipzig 7 (PHL7) variant R4M10
9QYC - Polyester Hydrolase Leipzig 7 (PHL7) variant R4M12
No kinetic
No disease
Database
Sequence
Peptide
MRHMSTSIAR VRTRLGALLA GVIVAGSTVI GAAPAGAQEN PYERGPDPTE SSIEAVRGPF AVAQTTVSRL QADGFGGGTI YYPTDTSQGT FGAVAISPGF TAGQESIAWL GPRIASQGFV VITIDTITRL DQPDSRGRQL QAALDHLRTN SVVRNRIDPN RMAVMGHSMG GGGALSAAAN NTSLEAAIPL QGWHTRKNWS SVRTPTLVVG AQLDTIAPVS SHSEAFYNSL PSDLDKAYME LRGASHLVSN TPDTTTAKYS IAWLKRFVDD DLRYEQFLCP APDDFAISEY RSTCPHSGSG GGTTPPPEDD CPWWAWWC
References (12)
| Title : Two-step biocatalytic conversion of post-consumer polyethylene terephthalate into value-added products facilitated by genetic and bioprocess engineering - Welsing_2025_Bioresour.Technol_417_131837 |
| Author(s) : Welsing G , Wolter B , Kleinert GEK , Gottsch F , Besenmatter W , Xue R , Mauri A , Steffens D , Kobbing S , Dong W , Jiang M , Bornscheuer UT , Wei R , Tiso T , Blank LM |
| Ref : Bioresour Technol , 417 :131837 , 2025 |
| Abstract : |
| PubMedSearch : Welsing_2025_Bioresour.Technol_417_131837 |
| PubMedID: 39557102 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Analysis of the product-spectrum during the biocatalytic hydrolysis of PEF (poly(ethylene furanoate)) with various esterases - Heinks_2025_RSC.Sustain__ |
| Author(s) : Heinks T , Hofmann K , Zimmermann L , Gamm I , Lieb A , Blach L , Wei R , Bornscheuer U.T , Thiele J , Hamel C , von Langermann J |
| Ref : RSC Sustainability , : , 2025 |
| Abstract : |
| PubMedSearch : Heinks_2025_RSC.Sustain__ |
| PubMedID: |
| Gene_locus related to this paper: deiml-e8u721 , 9bact-c3ryl0 , 9firm-PHL7 , 9bact-a0a2h5z9r5 , idesa-peth , 9bact-g9by57 , morsp-lip1 , thefu-q6a0i3 , sacvd-c7mve8 |
| Title : Activity of an anaerobic Thermoanaerobacterales hydrolase on aliphatic and aromatic polyesters - Siracusa_2024_Front.Bioeng.Biotechnol_12_1520680 |
| Author(s) : Siracusa C , Fohler L , Leibetseder L , Striedner G , Oostenbrink C , Quartinello F , Guebitz GM , Ribitsch D |
| Ref : Front Bioeng Biotechnol , 12 :1520680 , 2024 |
| Abstract : |
| PubMedSearch : Siracusa_2024_Front.Bioeng.Biotechnol_12_1520680 |
| PubMedID: 39898275 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Enabling high-throughput enzyme discovery and engineering with a low-cost, robot-assisted pipeline - Norton-Baker_2024_Sci.Rep_14_14449 |
| Author(s) : Norton-Baker B , Denton MCR , Murphy NP , Fram B , Lim S , Erickson E , Gauthier NP , Beckham GT |
| Ref : Sci Rep , 14 :14449 , 2024 |
| Abstract : |
| PubMedSearch : Norton-Baker_2024_Sci.Rep_14_14449 |
| PubMedID: 38914665 |
| Gene_locus related to this paper: 9actn-a0a1m7II12 , 9firm-PHL7 , 9bact-a0a2h5z9r5 , idesa-peth , 9bact-g9by57 , 9pseu-a0a1i6nu60 , thefu-q6a0i3 , sacvd-c7mve8 |
| Title : Unveiling PET Hydrolase Surface Dynamics through Fluorescence Microscopy - Rennison_2024_Chembiochem__e202300661 |
| Author(s) : Rennison AP , Nousi A , Westh P , Marie R , Moller MS |
| Ref : Chembiochem , :e202300661 , 2024 |
| Abstract : |
| PubMedSearch : Rennison_2024_Chembiochem__e202300661 |
| PubMedID: 38224131 |
| Gene_locus related to this paper: 9firm-PHL7 , 9bact-g9by57 |
| Title : Manufacturing of the highly active thermophile PETases PHL7 and PHL7mut3 using Escherichia coli - Fohler_2024_Microb.Cell.Fact_23_272 |
| Author(s) : Fohler L , Leibetseder L , Cserjan-Puschmann M , Striedner G |
| Ref : Microb Cell Fact , 23 :272 , 2024 |
| Abstract : |
| PubMedSearch : Fohler_2024_Microb.Cell.Fact_23_272 |
| PubMedID: 39390547 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : From Bulk to Binding: Decoding the Entry of PET into Hydrolase Binding Pockets - Jackering_2024_JACS.Au_4_4000 |
| Author(s) : Jackering A , Gottsch F , Schaffler M , Doerr M , Bornscheuer UT , Wei R , Strodel B |
| Ref : JACS Au , 4 :4000 , 2024 |
| Abstract : |
| PubMedSearch : Jackering_2024_JACS.Au_4_4000 |
| PubMedID: 39483243 |
| Gene_locus related to this paper: 9firm-PHL7 , 9bact-g9by57 |
| Title : One-Pot Depolymerization of Mixed Plastics Using a Dual Enzyme System - Wei_2024_ChemSusChem__e202402416 |
| Author(s) : Wei R , Branson Y , Liu J , Schmidt L , Griebel J , Prager A , Stieler L , Breite D , Bayer T , Besenmatter W , Dong W , Garscha U , Bornscheuer UT |
| Ref : ChemSusChem , :e202402416 , 2024 |
| Abstract : |
| PubMedSearch : Wei_2024_ChemSusChem__e202402416 |
| PubMedID: 39714869 |
| Gene_locus related to this paper: 9firm-PHL7 , pyrca-PCEST |
| Title : Structure and function of the metagenomic plastic-degrading polyester hydrolase PHL7 bound to its product - Richter_2023_Nat.Commun_14_1905 |
| Author(s) : Richter PK , Blazquez-Sanchez P , Zhao Z , Engelberger F , Wiebeler C , Kunze G , Frank R , Krinke D , Frezzotti E , Lihanova Y , Falkenstein P , Matysik J , Zimmermann W , Strater N , Sonnendecker C |
| Ref : Nat Commun , 14 :1905 , 2023 |
| Abstract : |
| PubMedSearch : Richter_2023_Nat.Commun_14_1905 |
| PubMedID: 37019924 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Low Carbon Footprint Recycling of Post-Consumer PET Plastic with a Metagenomic Polyester Hydrolase - Sonnendecker_2022_ChemSusChem_15_e202101062 |
| Author(s) : Sonnendecker C , Oeser J , Richter PK , Hille P , Zhao Z , Fischer C , Lippold H , Blazquez-Sanchez P , Engelberger F , Ramirez-Sarmiento CA , Oeser T , Lihanova Y , Frank R , Jahnke HG , Billig S , Abel B , Strater N , Matysik J , Zimmermann W |
| Ref : ChemSusChem , 15 :e202101062 , 2022 |
| Abstract : |
| PubMedSearch : Sonnendecker_2022_ChemSusChem_15_e202101062 |
| PubMedID: 34129279 |
| Gene_locus related to this paper: 9firm-PHL7 |
| Title : Real-Time Noninvasive Analysis of Biocatalytic PET Degradation - Frank_2022_ACS.Catal_12_25 |
| Author(s) : Frank R , Krinke D , Sonnendecker C , Zimmermann W , Jahnke HG |
| Ref : ACS Catal , 12 :25 , 2022 |
| Abstract : |
| PubMedSearch : Frank_2022_ACS.Catal_12_25 |
| PubMedID: |
| Gene_locus related to this paper: 9firm-PHL7 , 9bact-g9by57 |
| Title : Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase - Pfaff_2022_ACS.Catal_12_9790 |
| Author(s) : Pfaff L , Gao J , Li Z , Jackering A , Weber G , Mican J , Chen Y , Dong W , Han X , Feiler CG , Ao YF , Badenhorst CPS , Bednar D , Palm GJ , Lammers M , Damborsky J , Strodel B , Liu W , Bornscheuer UT , Wei R |
| Ref : ACS Catal , 12 :9790 , 2022 |
| Abstract : |
| PubMedSearch : Pfaff_2022_ACS.Catal_12_9790 |
| PubMedID: 35966606 |
| Gene_locus related to this paper: 9firm-PHL7 |