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sacvd-c7mve8

Saccharomonospora viridis PET-degrading cutinase Cut190 SvCut190. Cutinase

Comment

Other strains: Saccharomonospora viridis (strain ATCC 15386 \/ DSM 43017 \/ JCM 3036 \/ NBRC 12207 \/ P101)

Relationship

Family : Polyesterase-lipase-cutinase

Block : L

Position in NCBI Life Tree : Saccharomonospora viridis

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Bacteria N E > Terrabacteria group N E > Actinobacteria [phylum] N E > Actinobacteria [class] N E > Pseudonocardiales N E > Pseudonocardiaceae N E > Saccharomonospora N E > Saccharomonospora viridis N E

Molecular evidence

No mutation

4WFI - Crystal strucrure of PET-degrading cutinase Cut190 S226P mutant in Ca(2+)-free state 4WFJ - Crystal structure of PET-degrading cutinase Cut190 S226P mutant in Ca(2+)-bound state at 1.75 angstrom resolution 4WFK - Crystal structure of PET-degrading cutinase Cut190 S226P mutant in Ca(2+)-bound state at 2.35 angstrom resolution 5ZNO - Crystal structure of PET-degrading cutinase Cut190 S176A\/S226P\/R228S\/ mutant in Ca(2+)-bound state 5ZRQ - Crystal structure of PET-degrading cutinase Cut190 S176A\/S226P\/R228S mutant in Zn(2+)-bound state 5ZRR - Crystal structure of PET-degrading cutinase Cut190 S176A\/S226P\/R228S mutant in monoethyl succinate bound state 5ZRS - Crystal structure of PET-degrading cutinase Cut190 S176A\/S226P\/R228S mutant in monoethyl adipate bound state 7CEF - Crystal structure of PET-degrading cutinase Cut190 \/S226P\/R228S\/ mutant with the C-terminal three residues deletion 7CEH - Crystal structure of PET-degrading cutinase Cut190 S176A\/S226P\/R228S\/ mutant with the C-terminal three residues deletion in ligand ejecting form 7CTR - Closed form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P\/R228S\/Q138A\/D250C-E296C\/Q123H\/N202H Cut190*SS 7CTS - Open form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P\/R228S\/Q138A\/D250C-E296C\/Q123H\/N202H and S176A inactivation Cut190*SS_S176A 8IBL - MES bound form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P\/R228S\/Q138A\/D250C-E296C\/Q123H\/N202H and S176A inactivation 8IBM - Sulfate bound form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P\/R228S\/Q138A\/D250C-E296C\/Q123H\/N202H and S176A inactivation 8Z2G - MHET bound form of PET-degrading cutinase mutant Cut190*SS_S176A 8Z2H - Substrate analog a010 bound form of PET-degrading cutinase mutant Cut190**SS_S176A (A1L0N a10 4-(ethoxyphosphoryl)benzoic-acid) 8Z2I - Substrate analog a011 bound form of PET-degrading cutinase mutant Cut190**SS_S176A (A1L0L a11 4-(ethoxyphosphoryl)methylbenzoate) 8Z2J - Substrate analog a012 bound form of PET-degrading cutinase mutant Cut190**SS_S176A (A1L0M a12 4-(Phenoxyethylphosphoryl)methylbenzoate) 8Z2K - Substrate analog a013 bound form of PET-degrading cutinase mutant Cut190**SS_S176A in complex with (A1L0O a13 4-(Phenoxyethylphosphoryl)benzoate)

No kinetic

No disease

Database

Sequence

Peptide

MRIRRQAGTG ARASMARAIG VMTTALAVLV GAVGGVAGAE VSTAQDNPYE RGPDPTEDSI EAIRGPFSVA TERVSSFASG FGGGTIYYPR ETDEGTFGAV AVAPGFTASQ GSMSWYGERV ASQGFIVFTI DTNTRLDQPG QRGRQLLAAL DYLVERSDRK VRERLDPNRL AVMGHSMGGG GSLEATVMRP SLKASIPLTP WNLDKTWGQV QVPTFIIGAE LDTIASVRTH AKPFYESLPS SLPKAYMELD GATHFAPNIP NTTIAKYVIS WLKRFVDEDT RYSQFLCPNP TDRAIEEYRS TCPY

References (16)

Title : Structural dynamics of the Ca(2+)-regulated cutinase towards structure-based improvement of PET degradation activity - Numoto_2024_Int.J.Biol.Macromol_281_136597
Author(s) : Numoto N , Kondo F , Bekker GJ , Liao Z , Yamashita M , Iida A , Ito N , Kamiya N , Oda M
Ref : Int J Biol Macromol , 281 :136597 , 2024
Abstract :
PubMedSearch : Numoto_2024_Int.J.Biol.Macromol_281_136597
PubMedID: 39419144
Gene_locus related to this paper: sacvd-c7mve8

Title : Improvement of thermostability and activity of PET-degrading enzyme Cut190 towards a detailed understanding and application of the enzymatic reaction mechanism - Numoto_2023_bioRxiv__
Author(s) : Numoto N , Kamiya N , Oda M
Ref : Biorxiv , : , 2023
Abstract :
PubMedSearch : Numoto_2023_bioRxiv__
PubMedID:
Gene_locus related to this paper: sacvd-c7mve8

Title : Efficient depolymerization of polyethylene terephthalate (PET) and polyethylene furanoate by engineered PET hydrolase Cut190 - Kawai_2022_AMB.Express_12_134
Author(s) : Kawai F , Furushima Y , Mochizuki N , Muraki N , Yamashita M , Iida A , Mamoto R , Tosha T , Iizuka R , Kitajima S
Ref : AMB Express , 12 :134 , 2022
Abstract :
PubMedSearch : Kawai_2022_AMB.Express_12_134
PubMedID: 36289098
Gene_locus related to this paper: sacvd-c7mve8

Title : Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET - Magalhaes_2021_Int.J.Mol.Sci_22_11257
Author(s) : Magalhaes RP , Cunha JM , Sousa SF
Ref : Int J Mol Sci , 22 :11257 , 2021
Abstract :
PubMedSearch : Magalhaes_2021_Int.J.Mol.Sci_22_11257
PubMedID: 34681915
Gene_locus related to this paper: 9entr-EsEstB , 9bact-a0a2h5z9r5 , 9psed-peh , 9actn-h6wx58 , idesa-mheth , idesa-peth , thecd-d1a2h1 , humin-cut , 9acto-d4q9n1 , 9acto-f7ix06 , 9bact-g9by57 , bacsu-lip , bacsu-pnbae , canar-LipB , thela-m4tp71 , psemy-a4y035 , thefu-1831 , thefu-q6a0i4 , strsw-c9zcr8 , fusox-CUT , sacvd-c7mve8

Title : The Current State of Research on PET Hydrolyzing Enzymes Available for Biorecycling - Kawai_2021_Catalysts_11_206
Author(s) : Kawai F
Ref : Catalysts , 11 :206 , 2021
Abstract :
PubMedSearch : Kawai_2021_Catalysts_11_206
PubMedID:
Gene_locus related to this paper: 9acto-f7ix06 , sacvd-c7mve8

Title : Structural basis for Ca(2+)-dependent catalysis of a cutinase-like enzyme and its engineering: application to enzymatic PET depolymerization - Oda_2021_Biophys.Physicobiol_18_168
Author(s) : Oda M
Ref : Biophys Physicobiol , 18 :168 , 2021
Abstract :
PubMedSearch : Oda_2021_Biophys.Physicobiol_18_168
PubMedID: 34386313
Gene_locus related to this paper: sacvd-c7mve8

Title : Structural basis of mutants of PET-degrading enzyme from Saccharomonospora viridis AHK190 with high activity and thermal stability - Emori_2021_Proteins_89_502
Author(s) : Emori M , Numoto N , Senga A , Bekker GJ , Kamiya N , Kobayashi Y , Ito N , Kawai F , Oda M
Ref : Proteins , 89 :502 , 2021
Abstract :
PubMedSearch : Emori_2021_Proteins_89_502
PubMedID: 33340163
Gene_locus related to this paper: sacvd-c7mve8

Title : Multiple structural states of Ca2+-regulated PET hydrolase, Cut190, and its correlation with activity and stability - Senga_2021_J.Biochem_169_207
Author(s) : Senga A , Numoto N , Yamashita M , Iida A , Ito N , Kawai F , Oda M
Ref : J Biochem , 169 :207 , 2021
Abstract :
PubMedSearch : Senga_2021_J.Biochem_169_207
PubMedID: 32882044
Gene_locus related to this paper: sacvd-c7mve8

Title : Metal binding to cutinase-like enzyme from Saccharomonospora viridis AHK190 and its effects on enzyme activity and stability - Senga_2019_J.Biochem_166_149
Author(s) : Senga A , Hantani Y , Bekker GJ , Kamiya N , Kimura Y , Kawai F , Oda M
Ref : J Biochem , 166 :149 , 2019
Abstract :
PubMedSearch : Senga_2019_J.Biochem_166_149
PubMedID: 30825308
Gene_locus related to this paper: sacvd-c7mve8

Title : Folding thermodynamics of PET-hydrolyzing enzyme Cut190 depending on Ca2+ concentration - Inaba_2019_J.Therm.Anal.Calorim_135_2655
Author(s) : Inaba S , Kamiya N , Bekker GJ , Kawai F , Oda M
Ref : Journal of Thermal Analysis and Calorimetry , 135 :2655 , 2019
Abstract :
PubMedSearch : Inaba_2019_J.Therm.Anal.Calorim_135_2655
PubMedID:
Gene_locus related to this paper: sacvd-c7mve8

Title : Structural Dynamics of the PET-Degrading Cutinase-like Enzyme from Saccharomonospora viridis AHK190 in Substrate-Bound States Elucidates the Ca(2+)-Driven Catalytic Cycle - Numoto_2018_Biochemistry_57_5289
Author(s) : Numoto N , Kamiya N , Bekker GJ , Yamagami Y , Inaba S , Ishii K , Uchiyama S , Kawai F , Ito N , Oda M
Ref : Biochemistry , 57 :5289 , 2018
Abstract :
PubMedSearch : Numoto_2018_Biochemistry_57_5289
PubMedID: 30110540
Gene_locus related to this paper: sacvd-c7mve8

Title : Enzymatic hydrolysis of PET: functional roles of three Ca(2+) ions bound to a cutinase-like enzyme, Cut190*, and its engineering for improved activity - Oda_2018_Appl.Microbiol.Biotechnol_102_10067
Author(s) : Oda M , Yamagami Y , Inaba S , Oida T , Yamamoto M , Kitajima S , Kawai F
Ref : Applied Microbiology & Biotechnology , 102 :10067 , 2018
Abstract :
PubMedSearch : Oda_2018_Appl.Microbiol.Biotechnol_102_10067
PubMedID: 30250976
Gene_locus related to this paper: sacvd-c7mve8

Title : Mutational analysis of cutinase-like enzyme, Cut190, based on the 3D docking structure with model compounds of polyethylene terephthalate - Kawabata_2017_J.Biosci.Bioeng_124_28
Author(s) : Kawabata T , Oda M , Kawai F
Ref : J Biosci Bioeng , 124 :28 , 2017
Abstract :
PubMedSearch : Kawabata_2017_J.Biosci.Bioeng_124_28
PubMedID: 28259444
Gene_locus related to this paper: sacvd-c7mve8

Title : Structural basis for the Ca(2+)-enhanced thermostability and activity of PET-degrading cutinase-like enzyme from Saccharomonospora viridis AHK190 - Miyakawa_2015_Appl.Microbiol.Biotechnol_99_4297
Author(s) : Miyakawa T , Mizushima H , Ohtsuka J , Oda M , Kawai F , Tanokura M
Ref : Applied Microbiology & Biotechnology , 99 :4297 , 2015
Abstract :
PubMedSearch : Miyakawa_2015_Appl.Microbiol.Biotechnol_99_4297
PubMedID: 25492421
Gene_locus related to this paper: sacvd-c7mve8

Title : A novel Ca-activated, thermostabilized polyesterase capable of hydrolyzing polyethylene terephthalate from Saccharomonospora viridis AHK190 - Kawai_2014_Appl.Microbiol.Biotechnol_98_10053
Author(s) : Kawai F , Oda M , Tamashiro T , Waku T , Tanaka N , Yamamoto M , Mizushima H , Miyakawa T , Tanokura M
Ref : Applied Microbiology & Biotechnology , 98 :10053 , 2014
Abstract :
PubMedSearch : Kawai_2014_Appl.Microbiol.Biotechnol_98_10053
PubMedID: 24929560
Gene_locus related to this paper: sacvd-c7mve8

Title : Complete genome sequence of Saccharomonospora viridis type strain (P101) - Pati_2009_Stand.Genomic.Sci_1_141
Author(s) : Pati A , Sikorski J , Nolan M , Lapidus A , Copeland A , Glavina Del Rio T , Lucas S , Chen F , Tice H , Pitluck S , Cheng JF , Chertkov O , Brettin T , Han C , Detter JC , Kuske C , Bruce D , Goodwin L , Chain P , D'Haeseleer P , Chen A , Palaniappan K , Ivanova N , Mavromatis K , Mikhailova N , Rohde M , Tindall BJ , Goker M , Bristow J , Eisen JA , Markowitz V , Hugenholtz P , Kyrpides NC , Klenk HP
Ref : Stand Genomic Sci , 1 :141 , 2009
Abstract :
PubMedSearch : Pati_2009_Stand.Genomic.Sci_1_141
PubMedID: 21304650
Gene_locus related to this paper: sacvd-DsvA , sacvd-c7mpm7 , sacvd-c7mpv6 , sacvd-c7mqn5 , sacvd-c7mrh9 , sacvd-c7mrj7 , sacvd-c7msh1 , sacvd-c7mss4 , sacvd-c7msy5 , sacvd-c7mua8 , sacvd-c7mv20 , sacvd-c7mvm9 , sacvd-c7mx36 , sacvd-c7my02 , sacvd-c7myf1 , sacvd-c7myf2 , sacvd-c7myh3 , sacvd-c7myv3 , sacvd-c7mzb0 , sacvd-c7n0e5 , sacvd-c7mxx2 , sacvd-c7mwe5 , sacvd-c7mve8 , sacvd-c7mu02 , sacvd-c7myq6