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hevbr-hnl

Hevea brasiliensis hydroxynitrile lyase (hnl) HbHNL mRNA, complete cds

Comment

Rubber tree hydroxynitrile lyase HbHNL. this enzyme is close to plant esterase SABP2. Few mutations can convert one activity to the other (Padhi et al. Nedrud et al.)

Relationship

Family : Hydroxynitrile_lyase

Block : X

Position in NCBI Life Tree : Hevea brasiliensis

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Eukaryota N E > Viridiplantae N E > Streptophyta N E > Streptophytina N E > Embryophyta N E > Tracheophyta N E > Euphyllophyta N E > Spermatophyta N E > Magnoliophyta N E > Mesangiospermae N E > eudicotyledons N E > Gunneridae N E > Pentapetalae N E > rosids N E > fabids N E > Malpighiales N E > Euphorbiaceae N E > Crotonoideae N E > Micrandreae N E > Hevea N E > Hevea brasiliensis N E

Molecular evidence

No mutation

1QJ4 - Hydroxynitrile lyase complexed with gol, so4 1SC9 - Hydroxynitrile Lyase from Hevea brasiliensis in complex with the natural substrate acetone cyanohydrin 1SCI - K236L mutant of hydroxynitrile lyase from Hevea brasiliensis 1SCK - K236L mutant of hydroxynitrile lyase from Hevea brasiliensis in complex with acetone 1SCQ - K236L mutant of hydroxynitrile lyase from Hevea brasiliensis in complex with acetonecyanohydrin 1YAS - Rubber tree hydroxynitrile lyase + histidine 1YB6 - Hydroxynitrile lyase from hevea brasiliensis in complex with mandelonitrile 1YB7 - Hydroxynitrile lyase from hevea brasiliensis in complex with 2,3-dimethyl-2-hydroxy-butyronitrile 2YAS - hydroxynitrile lyase + rhodanide 3C6X - Hydroxynitrile lyase HNL from Hevea brasiliensis to atomic resolution native with PEG200 3C6Y - Hydroxynitrile lyase HNL from Hevea brasiliensis to atomic resolution, complex with acetone 3C6Z - Hydroxynitrile lyase HNL from Hevea brasiliensis to atomic resolution, complex with isopropanol 3C70 - Hydroxynitrile lyase HNL from Hevea brasiliensis to atomic resolution, complex with rhodanide (SCN) 3YAS - Hydroxynitrile lyase + acetone 4YAS - Hydroxynitrile lyase + chloralhydrate 5YAS - Hydroxynitrile lyase + hexafluoroacetone 6YAS - Hydroxynitrile lyase native room temperature 7YAS - Hydroxynitrile lyase native low temperature 8EUO - Hydroxynitrile lyase HNL from Hevea brasiliensis with Seven Mutations 8SNI - Hydroxynitrile Lyase from Hevea brasiliensis with Forty Mutations 9CLR - Hydroxynitrile lyase HNL from Hevea brasiliensis with Seventy-one Mutations

No kinetic

No disease

Database

Sequence

Peptide

MAFAHFVLIH TICHGAWIWH KLKPLLEALG HKVTALDLAA SGVDPRQIEE IGSFDEYSEP LLTFLEALPP GEKVILVGES CGGLNIAIAA DKYCEKIAAA VFHNSVLPDT EHCPSYVVDK LMEVFPDWKD TTYFTYTKDG KEITGLKLGF TLLRENLYTL CGPEEYELAK MLTRKGSLFQ NILAKRPFFT KEGYGSIKKI YVWTDQDEIF LPEFQLWQIE NYKPDKVYKV EGGDHKLQLT KTKEIAEILQ EVADTYN

References (15)

Title : Designing Efficient Enzymes: Eight Predicted Mutations Convert a Hydroxynitrile Lyase into an Efficient Esterase - Casadevall_2023_bioRxiv__
Author(s) : Casadevall G , Pierce C , Guan B , Iglesias-Fernandez J , Lim HY , Greenberg LR , Walsh ME , Shi K , Gordon W , Aihara H , Evans RL , Kazlauskas R , Osuna S
Ref : Biorxiv , : , 2023
Abstract :
PubMedSearch : Casadevall_2023_bioRxiv__
PubMedID: 37662272
Gene_locus related to this paper: hevbr-hnl

Title : A De Novo Designed Esterase with p-Nitrophenyl Acetate Hydrolysis Activity - Li_2020_Molecules_25_4658
Author(s) : Li G , Xu L , Zhang H , Liu J , Yan J , Yan Y
Ref : Molecules , 25 :4658 , 2020
Abstract :
PubMedSearch : Li_2020_Molecules_25_4658
PubMedID: 33066055
Gene_locus related to this paper: aspor-cutas , fusso-cutas , hevbr-hnl

Title : Identical active sites in hydroxynitrile lyases show opposite enantioselectivity and reveal possible ancestral mechanism - Jones_2017_ACS.Catal_7_4221
Author(s) : Jones BJ , Bata Z , Kazlauskas RJ
Ref : ACS Catal , 7 :4221 , 2017
Abstract :
PubMedSearch : Jones_2017_ACS.Catal_7_4221
PubMedID: 28798888
Gene_locus related to this paper: arath-HNL , hevbr-hnl

Title : Evolution of a Catalytic Mechanism - Rauwerdink_2016_Mol.Biol.Evol_33_971
Author(s) : Rauwerdink A , Lunzer M , Devamani T , Jones B , Mooney J , Zhang ZJ , Xu JH , Kazlauskas RJ , Dean AM
Ref : Molecular Biology Evolution , 33 :971 , 2016
Abstract :
PubMedSearch : Rauwerdink_2016_Mol.Biol.Evol_33_971
PubMedID: 26681154
Gene_locus related to this paper: 9zzzz-5TDX , hevbr-hnl

Title : Uncovering divergent evolution of alpha\/beta-hydrolases: a surprising residue substitution needed to convert hydroxynitrile lyase into an esterase - Nedrud_2014_Chem.Sci_5_4265
Author(s) : Nedrud DM , Lin H , Lopez G , Padhi SK , Legatt GA , Kazlauskas RJ
Ref : Chem Sci , 5 :4265 , 2014
Abstract :
PubMedSearch : Nedrud_2014_Chem.Sci_5_4265
PubMedID: 25346843
Gene_locus related to this paper: hevbr-hnl , nicta-SABP2

Title : Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions - Padhi_2010_Chem.Biol_17_863
Author(s) : Padhi SK , Fujii R , Legatt GA , Fossum SL , Berchtold R , Kazlauskas RJ
Ref : Chemical Biology , 17 :863 , 2010
Abstract :
PubMedSearch : Padhi_2010_Chem.Biol_17_863
PubMedID: 20797615
Gene_locus related to this paper: hevbr-hnl , nicta-SABP2

Title : Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha\/beta-hydrolase fold - Guterl_2009_J.Biotechnol_141_166
Author(s) : Guterl JK , Andexer JN , Sehl T , von Langermann J , Frindi-Wosch I , Rosenkranz T , Fitter J , Gruber K , Kragl U , Eggert T , Pohl M
Ref : J Biotechnol , 141 :166 , 2009
Abstract :
PubMedSearch : Guterl_2009_J.Biotechnol_141_166
PubMedID: 19433222
Gene_locus related to this paper: arath-HNL , hevbr-hnl , manes-hnl

Title : Atomic resolution crystal structures and quantum chemistry meet to reveal subtleties of hydroxynitrile lyase catalysis - Schmidt_2008_J.Biol.Chem_283_21827
Author(s) : Schmidt A , Gruber K , Kratky C , Lamzin VS
Ref : Journal of Biological Chemistry , 283 :21827 , 2008
Abstract :
PubMedSearch : Schmidt_2008_J.Biol.Chem_283_21827
PubMedID: 18524775
Gene_locus related to this paper: hevbr-hnl

Title : Structural determinants of the enantioselectivity of the hydroxynitrile lyase from Hevea brasiliensis - Gartler_2007_J.Biotechnol_129_87
Author(s) : Gartler G , Kratky C , Gruber K
Ref : J Biotechnol , 129 :87 , 2007
Abstract :
PubMedSearch : Gartler_2007_J.Biotechnol_129_87
PubMedID: 17250917
Gene_locus related to this paper: hevbr-hnl

Title : Reaction mechanism of hydroxynitrile lyases of the alpha\/beta-hydrolase superfamily: the three-dimensional structure of the transient enzyme-substrate complex certifies the crucial role of LYS236 - Gruber_2004_J.Biol.Chem_279_20501
Author(s) : Gruber K , Gartler G , Krammer B , Schwab H , Kratky C
Ref : Journal of Biological Chemistry , 279 :20501 , 2004
Abstract :
PubMedSearch : Gruber_2004_J.Biol.Chem_279_20501
PubMedID: 14998991
Gene_locus related to this paper: hevbr-hnl

Title : Three-dimensional structures of enzyme-substrate complexes of the hydroxynitrile lyase from Hevea brasiliensis - Zuegg_1999_Protein.Sci_8_1990
Author(s) : Zuegg J , Gruber K , Gugganig M , Wagner UG , Kratky C
Ref : Protein Science , 8 :1990 , 1999
Abstract :
PubMedSearch : Zuegg_1999_Protein.Sci_8_1990
PubMedID: 10548044
Gene_locus related to this paper: hevbr-hnl

Title : Hydroxynitrile lyase from Hevea brasiliensis: molecular characterization and mechanism of enzyme catalysis - Hasslacher_1997_Proteins_27_438
Author(s) : Hasslacher M , Kratky C , Griengl H , Schwab H , Kohlwein SD
Ref : Proteins , 27 :438 , 1997
Abstract :
PubMedSearch : Hasslacher_1997_Proteins_27_438
PubMedID: 9094745
Gene_locus related to this paper: hevbr-hnl

Title : Molecular cloning of the full-length cDNA of (S)-hydroxynitrile lyase from Hevea brasiliensis. Functional expression in Escherichia coli and Saccharomyces cerevisiae and identification of an active site residue - Hasslacher_1996_J.Biol.Chem_271_5884
Author(s) : Hasslacher M , Schall M , Hayn M , Griengl H , Kohlwein SD , Schwab H
Ref : Journal of Biological Chemistry , 271 :5884 , 1996
Abstract :
PubMedSearch : Hasslacher_1996_J.Biol.Chem_271_5884
PubMedID: 8621461
Gene_locus related to this paper: hevbr-hnl

Title : (S)-hydroxynitrile lyase from Hevea brasiliensis -
Author(s) : Hasslacher M , Schall M , Hayn M , Griengl H , Kohlwein SD , Schwab H
Ref : Annals of the New York Academy of Sciences , 799 :707 , 1996
PubMedID: 8958122
Gene_locus related to this paper: hevbr-hnl

Title : Mechanism of cyanogenesis: the crystal structure of hydroxynitrile lyase from Hevea brasiliensis - Wagner_1996_Structure_4_811
Author(s) : Wagner UG , Hasslacher M , Griengl H , Schwab H , Kratky C
Ref : Structure , 4 :811 , 1996
Abstract :
PubMedSearch : Wagner_1996_Structure_4_811
PubMedID: 8805565
Gene_locus related to this paper: hevbr-hnl