aspor-cutas

Aspergillus oryzae (Yellow koji mold), Aspergillus flavus, CutL1 gene for cutinase cutinase 1 precursor (EC 3.1.1.74) (cutin hydrolase 1) (l1)

Comment

Aspergillus oryzae grown in a liquid medium containing the polyester polybutylene succinate co-adipate (PBSA), produces RolA, a hydrophobin, and CutL1, a PBSA-degrading cutinase. Secreted RolA attaches to PBSA particles and recruits CutL1, which stimulates the hydrolysis of PBSA. Asp142, Asp171, Glu31 and Asp30 are involved in the ionic interaction with RolA CutL1 (Takahashi et al.2015, Terauchi 2017). Structures of CutL alone or in complex with paraoxon were solved(Liu et al. 2009). Other strains: Aspergillus oryzae (strain ATCC 42149 \/ RIB 40) (Yellow koji mold), Aspergillus flavus (strain ATCC 200026\/FGSC A1120\/NRRL 3357\/JC12722\/SRRC 167)

Relationship

Family : Cutinase

Block : X

Position in NCBI Life Tree : Aspergillus oryzae

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Eukaryota N E > Opisthokonta N E > Fungi N E > Dikarya N E > Ascomycota N E > saccharomyceta N E > Pezizomycotina N E > leotiomyceta N E > Eurotiomycetes N E > Eurotiomycetidae N E > Eurotiales N E > Aspergillaceae N E > Aspergillus N E > Aspergillus oryzae N E

Database

Sequence

Peptide

MHLRNIVIAL AATAVASPVD LQDRQLTGGD ELRDGPCKPI TFIFARASTE PGLLGISTGP AVCNRLKLAR SGDVACQGVG PRYTADLPSN ALPEGTSQAA IAEAQGLFEQ AVSKCPDTQI VAGGYSQGTA VMNGAIKRLS ADVQDKIKGV VLFGYTRNAQ ERGQIANFPK DKVKVYCAVG DLVCLGTLIV APPHFSYLSD TGDASDFLLS QLG

References (9)

Title : A De Novo Designed Esterase with p-Nitrophenyl Acetate Hydrolysis Activity - Li_2020_Molecules_25_4658
Author(s) : Li G , Xu L , Zhang H , Liu J , Yan J , Yan Y
Ref : Molecules , 25 :4658 , 2020
PubMedID: 33066055
Gene_locus related to this paper: aspor-cutas , fusso-cutas , hevbr-hnl

Title : Asp30 of Aspergillus oryzae cutinase CutL1 is involved in the ionic interaction with fungal hydrophobin RolA - Terauchi_2017_Biosci.Biotechnol.Biochem_81_1363
Author(s) : Terauchi Y , Kim YK , Tanaka T , Nanatani K , Takahashi T , Abe K
Ref : Biosci Biotechnol Biochem , :1 , 2017
PubMedID: 28475418
Gene_locus related to this paper: aspor-cutas

Title : Toward rational thermostabilization of Aspergillus oryzae cutinase: Insights into catalytic and structural stability - Shirke_2016_Proteins_84_60
Author(s) : Shirke AN , Basore D , Butterfoss GL , Bonneau R , Bystroff C , Gross RA
Ref : Proteins , 84 :60 , 2016
PubMedID: 26522152
Gene_locus related to this paper: aspor-cutas

Title : Ionic interaction of positive amino acid residues of fungal hydrophobin RolA with acidic amino acid residues of cutinase CutL1 - Takahashi_2015_Mol.Microbiol_96_14
Author(s) : Takahashi T , Tanaka T , Tsushima Y , Muragaki K , Uehara K , Takeuchi S , Maeda H , Yamagata Y , Nakayama M , Yoshimi A , Abe K
Ref : Molecular Microbiology , 96 :14 , 2015
PubMedID: 25588312
Gene_locus related to this paper: aspor-cutas

Title : Structural and functional studies of Aspergillus oryzae cutinase: enhanced thermostability and hydrolytic activity of synthetic ester and polyester degradation - Liu_2009_J.Am.Chem.Soc_131_15711
Author(s) : Liu Z , Gosser Y , Baker PJ , Ravee Y , Lu Z , Alemu G , Li H , Butterfoss GL , Kong XP , Gross R , Montclare JK
Ref : Journal of the American Chemical Society , 131 :15711 , 2009
PubMedID: 19810726
Gene_locus related to this paper: aspor-cutas

Title : Genome sequencing and analysis of Aspergillus oryzae - Machida_2005_Nature_438_1157
Author(s) : Machida M , Asai K , Sano M , Tanaka T , Kumagai T , Terai G , Kusumoto K , Arima T , Akita O , Kashiwagi Y , Abe K , Gomi K , Horiuchi H , Kitamoto K , Kobayashi T , Takeuchi M , Denning DW , Galagan JE , Nierman WC , Yu J , Archer DB , Bennett JW , Bhatnagar D , Cleveland TE , Fedorova ND , Gotoh O , Horikawa H , Hosoyama A , Ichinomiya M , Igarashi R , Iwashita K , Juvvadi PR , Kato M , Kato Y , Kin T , Kokubun A , Maeda H , Maeyama N , Maruyama J , Nagasaki H , Nakajima T , Oda K , Okada K , Paulsen I , Sakamoto K , Sawano T , Takahashi M , Takase K , Terabayashi Y , Wortman JR , Yamada O , Yamagata Y , Anazawa H , Hata Y , Koide Y , Komori T , Koyama Y , Minetoki T , Suharnan S , Tanaka A , Isono K , Kuhara S , Ogasawara N , Kikuchi H
Ref : Nature , 438 :1157 , 2005
PubMedID: 16372010
Gene_locus related to this paper: aspor-Q2U722 , aspfn-b8mvx2 , aspfn-b8mwk1 , aspfn-b8n1a4 , aspfn-b8n5l3 , aspfn-b8n7y0 , aspfn-b8n829 , aspfn-b8ncj5 , aspfn-b8nhj9 , aspfn-b8njx6 , aspfn-b8nsk2 , aspfu-q4wj61 , aspor-axe1 , aspor-CPI , aspor-cutas , aspor-cuti2 , aspor-DPPIV , aspor-faec , aspor-MDLB , aspor-ppme1 , aspor-q2tw11 , aspor-q2tw16 , aspor-q2tw28 , aspor-q2twc4 , aspor-q2twg0 , aspor-q2twj3 , aspor-q2twv2 , aspor-q2twv4 , aspor-q2tx21 , aspor-q2txq8 , aspor-q2tya1 , aspor-q2tyh6 , aspor-q2tyn9 , aspor-q2typ0 , aspor-q2tyq4 , aspor-q2tyv8 , aspor-q2tz03 , aspor-q2tzh3 , aspor-q2tzr5 , aspor-q2tzv9 , aspor-q2u0k7 , aspor-q2u0q2 , aspor-q2u0r6 , aspor-q2u1a5 , aspor-q2u1a6 , aspor-q2u1k0 , aspor-q2u1k8 , aspor-q2u1m8 , aspor-q2u2a1 , aspor-q2u2a4 , aspor-q2u3a3 , aspor-q2u3a6 , aspor-q2u3k5 , aspor-q2u3l6 , aspor-q2u4a0 , aspor-q2u4e0 , aspor-q2u4f6 , aspor-q2u4g6 , aspor-q2u4h9 , aspor-q2u4w9 , aspor-q2u4y8 , aspor-q2u5f5 , aspor-q2u5n3 , aspor-q2u5y8 , aspor-q2u6h7 , aspor-q2u6j5 , aspor-q2u6m8 , aspor-q2u6m9 , aspor-q2u6n6 , aspor-q2u7i2 , aspor-q2u7v0 , aspor-q2u8j8 , aspor-q2u8r1 , aspor-q2u8r4 , aspor-q2u8t5 , aspor-q2u8z3 , aspor-q2u9a1 , aspor-q2u9n5 , aspor-q2u144 , aspor-q2u161 , aspor-q2u185 , aspor-q2u199 , aspor-q2u212 , aspor-q2u331 , aspor-q2u348 , aspor-q2u400 , aspor-q2u453 , aspor-q2u489 , aspor-q2u704 , aspor-q2u728 , aspor-q2u798 , aspor-q2u822 , aspor-q2u854 , aspor-q2u875 , aspor-q2u908 , aspor-q2ua10 , aspor-q2ua48 , aspor-q2uab6 , aspor-q2uak9 , aspor-q2uaq4 , aspor-q2ub32 , aspor-q2ub76 , aspor-q2uba1 , aspor-q2ubd6 , aspor-q2ubm2 , aspor-q2ubr2 , aspor-q2uc28 , aspor-q2uc65 , aspor-q2uc77 , aspor-q2uc98 , aspor-q2uck0 , aspor-q2ucy7 , aspor-q2ud03 , aspor-q2ud06 , aspor-q2ud08 , aspor-q2ud23 , aspor-q2udn5 , aspor-q2udr0 , aspor-q2uec1 , aspor-q2uef3 , aspor-q2uf10 , aspor-q2uf27 , aspor-q2uf48 , aspor-q2ufd8 , aspor-q2ufe5 , aspor-q2ufm4 , aspor-q2ufr3 , aspor-q2ufz8 , aspor-q2ug78 , aspor-q2ugd6 , aspor-q2uge1 , aspor-q2ugg7 , aspor-q2ugi2 , aspor-q2ugl2 , aspor-q2ugy9 , aspor-q2uh24 , aspor-q2uh73 , aspor-q2uhe4 , aspor-q2uhf0 , aspor-q2uhj6 , aspor-q2uhn1 , aspor-q2uhq0 , aspor-q2ui56 , aspor-q2uib2 , aspor-q2uib5 , aspor-q2uie9 , aspor-q2uih1 , aspor-q2uii1 , aspor-q2uik9 , aspor-q2uiq0 , aspor-q2uiu1 , aspor-q2uix9 , aspor-q2uiy5 , aspor-q2uiz4 , aspor-q2uj89 , aspor-q2uja2 , aspor-q2uju3 , aspor-q2uk31 , aspor-q2uk42 , aspor-q2ukb6 , aspor-q2ukq7 , aspor-q2ul81 , aspor-q2uli9 , aspor-q2ulr2 , aspor-q2ulv7 , aspor-q2umf3 , aspor-q2umv2 , aspor-q2umx6 , aspor-q2unw5 , aspor-q2up23 , aspor-q2up89 , aspor-q2upe6 , aspor-q2upi1 , aspor-q2upl1 , aspor-q2upw4 , aspor-q2uq56 , aspor-q2uqb4 , aspor-q2uqm7 , aspor-q2ur58 , aspor-q2ur64 , aspor-q2ur80 , aspor-q2ur83 , aspor-q2ure7 , aspor-q2urf3 , aspor-q2urg5 , aspor-q2urq0 , aspor-q2urt4 , aspor-q2uru5 , aspor-q2usi0 , aspor-q2usp7 , aspor-q2usq8 , aspor-q2usv6 , aspor-q2uta5 , aspor-q2uu89 , aspor-q2uub4 , aspor-q2uux8 , aspor-q2uv29 , aspor-TGLA , aspor-q2ue03 , aspor-q2uj83 , aspno-a0a0l1j1c9

Title : The fungal hydrophobin RolA recruits polyesterase and laterally moves on hydrophobic surfaces - Takahashi_2005_Mol.Microbiol_57_1780
Author(s) : Takahashi T , Maeda H , Yoneda S , Ohtaki S , Yamagata Y , Hasegawa F , Gomi K , Nakajima T , Abe K
Ref : Molecular Microbiology , 57 :1780 , 2005
PubMedID: 16135240
Gene_locus related to this paper: aspor-cutas

Title : Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzae - Maeda_2005_Appl.Microbiol.Biotechnol_67_778
Author(s) : Maeda H , Yamagata Y , Abe K , Hasegawa F , Machida M , Ishioka R , Gomi K , Nakajima T
Ref : Applied Microbiology & Biotechnology , 67 :778 , 2005
PubMedID: 15968570
Gene_locus related to this paper: aspor-cutas

Title : Genome structure and nucleotide sequence of a lipolytic enzyme gene of Aspergillus oryzae - Ohnishi_1995_FEMS.Microbiol.Lett_126_145
Author(s) : Ohnishi K , Toida J , Nakazawa H , Sekiguchi J
Ref : FEMS Microbiology Letters , 126 :145 , 1995
PubMedID: 7705606
Gene_locus related to this paper: aspor-cutas