Aspergillus oryzae (Yellow koji mold), Aspergillus flavus, CutL1 gene for cutinase cutinase 1 precursor (EC 3.1.1.74) (cutin hydrolase 1) (l1)
Aspergillus oryzae grown in a liquid medium containing the polyester polybutylene succinate co-adipate (PBSA), produces RolA, a hydrophobin, and CutL1, a PBSA-degrading cutinase. Secreted RolA attaches to PBSA particles and recruits CutL1, which stimulates the hydrolysis of PBSA. Asp142, Asp171, Glu31 and Asp30 are involved in the ionic interaction with RolA CutL1 (Takahashi et al.2015, Terauchi 2017). Structures of CutL alone or in complex with paraoxon were solved(Liu et al. 2009). Other strains: Aspergillus oryzae (strain ATCC 42149 \/ RIB 40) (Yellow koji mold), Aspergillus flavus (strain ATCC 200026\/FGSC A1120\/NRRL 3357\/JC12722\/SRRC 167)
Family : Cutinase
Block : X
Position in NCBI Life Tree : Aspergillus oryzae
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Eukaryota N E > Opisthokonta N E > Fungi N E > Dikarya N E > Ascomycota N E > saccharomyceta N E > Pezizomycotina N E > leotiomyceta N E > Eurotiomycetes N E > Eurotiomycetidae N E > Eurotiales N E > Aspergillaceae N E > Aspergillus N E > Aspergillus oryzae N EMHLRNIVIAL AATAVASPVD LQDRQLTGGD ELRDGPCKPI TFIFARASTE PGLLGISTGP AVCNRLKLAR SGDVACQGVG PRYTADLPSN ALPEGTSQAA IAEAQGLFEQ AVSKCPDTQI VAGGYSQGTA VMNGAIKRLS ADVQDKIKGV VLFGYTRNAQ ERGQIANFPK DKVKVYCAVG DLVCLGTLIV APPHFSYLSD TGDASDFLLS QLG
Title : A De Novo Designed Esterase with p-Nitrophenyl Acetate Hydrolysis Activity - Li_2020_Molecules_25_4658 |
Author(s) : Li G , Xu L , Zhang H , Liu J , Yan J , Yan Y |
Ref : Molecules , 25 :4658 , 2020 |
Abstract : |
PubMedSearch : Li_2020_Molecules_25_4658 |
PubMedID: 33066055 |
Gene_locus related to this paper: aspor-cutas , fusso-cutas , hevbr-hnl |
Title : Asp30 of Aspergillus oryzae cutinase CutL1 is involved in the ionic interaction with fungal hydrophobin RolA - Terauchi_2017_Biosci.Biotechnol.Biochem_81_1363 |
Author(s) : Terauchi Y , Kim YK , Tanaka T , Nanatani K , Takahashi T , Abe K |
Ref : Biosci Biotechnol Biochem , :1 , 2017 |
Abstract : |
PubMedSearch : Terauchi_2017_Biosci.Biotechnol.Biochem_81_1363 |
PubMedID: 28475418 |
Gene_locus related to this paper: aspor-cutas |
Title : Toward rational thermostabilization of Aspergillus oryzae cutinase: Insights into catalytic and structural stability - Shirke_2016_Proteins_84_60 |
Author(s) : Shirke AN , Basore D , Butterfoss GL , Bonneau R , Bystroff C , Gross RA |
Ref : Proteins , 84 :60 , 2016 |
Abstract : |
PubMedSearch : Shirke_2016_Proteins_84_60 |
PubMedID: 26522152 |
Gene_locus related to this paper: aspor-cutas |
Title : Ionic interaction of positive amino acid residues of fungal hydrophobin RolA with acidic amino acid residues of cutinase CutL1 - Takahashi_2015_Mol.Microbiol_96_14 |
Author(s) : Takahashi T , Tanaka T , Tsushima Y , Muragaki K , Uehara K , Takeuchi S , Maeda H , Yamagata Y , Nakayama M , Yoshimi A , Abe K |
Ref : Molecular Microbiology , 96 :14 , 2015 |
Abstract : |
PubMedSearch : Takahashi_2015_Mol.Microbiol_96_14 |
PubMedID: 25588312 |
Gene_locus related to this paper: aspor-cutas |
Title : Structural and functional studies of Aspergillus oryzae cutinase: enhanced thermostability and hydrolytic activity of synthetic ester and polyester degradation - Liu_2009_J.Am.Chem.Soc_131_15711 |
Author(s) : Liu Z , Gosser Y , Baker PJ , Ravee Y , Lu Z , Alemu G , Li H , Butterfoss GL , Kong XP , Gross R , Montclare JK |
Ref : Journal of the American Chemical Society , 131 :15711 , 2009 |
Abstract : |
PubMedSearch : Liu_2009_J.Am.Chem.Soc_131_15711 |
PubMedID: 19810726 |
Gene_locus related to this paper: aspor-cutas |
Title : The fungal hydrophobin RolA recruits polyesterase and laterally moves on hydrophobic surfaces - Takahashi_2005_Mol.Microbiol_57_1780 |
Author(s) : Takahashi T , Maeda H , Yoneda S , Ohtaki S , Yamagata Y , Hasegawa F , Gomi K , Nakajima T , Abe K |
Ref : Molecular Microbiology , 57 :1780 , 2005 |
Abstract : |
PubMedSearch : Takahashi_2005_Mol.Microbiol_57_1780 |
PubMedID: 16135240 |
Gene_locus related to this paper: aspor-cutas |
Title : Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzae - Maeda_2005_Appl.Microbiol.Biotechnol_67_778 |
Author(s) : Maeda H , Yamagata Y , Abe K , Hasegawa F , Machida M , Ishioka R , Gomi K , Nakajima T |
Ref : Applied Microbiology & Biotechnology , 67 :778 , 2005 |
Abstract : |
PubMedSearch : Maeda_2005_Appl.Microbiol.Biotechnol_67_778 |
PubMedID: 15968570 |
Gene_locus related to this paper: aspor-cutas |
Title : Genome structure and nucleotide sequence of a lipolytic enzyme gene of Aspergillus oryzae - Ohnishi_1995_FEMS.Microbiol.Lett_126_145 |
Author(s) : Ohnishi K , Toida J , Nakazawa H , Sekiguchi J |
Ref : FEMS Microbiology Letters , 126 :145 , 1995 |
Abstract : |
PubMedSearch : Ohnishi_1995_FEMS.Microbiol.Lett_126_145 |
PubMedID: 7705606 |
Gene_locus related to this paper: aspor-cutas |