human-SCPEP1

Homo sapiens (Human) serine Retinoid-inducible serine carboxypeptidase RISC SCP1 (EC 3.4.16.-)

Comment

Homo sapiens (Human) dc39 Trembl Q9H2J9 mstp034 Q9H3F0 Serine carboxypeptidase 1 old human-HSCP1. Kollmann et al. (2009) showed that the 55-kD mouse Scpep1 precursor protein was processed into a 35-kD N-terminal fragment and an 18-kD C-terminal fragment. Processing was mediated by a lysosomal serine proteinase and took place in the endosomal-lysosomal compartment. All 5 putative N-glycosylation sites of Scpep1 were N-glycosylated in mouse embryonic fibroblasts. Lee et al. (2009) showed that Scpep1 expression promoted growth and migration of mouse or rat smooth muscle cells (SMCs). Mutation analysis revealed that the stimulatory effects of Scpep1 on SMC growth and migration required the Scpep1 catalytic triad, but not cleavage of Scpep1. Immunohistochemical analysis of injured mouse and rat carotid arteries demonstrated increased Scpep1 expression in vessel walls where modulated SMCs were proliferative and migratory. By analyzing cultured mouse aortic vascular SMCs (AVSMCs), Pan et al. (2014) showed that Scpep1 had carboxypeptidase activity towards ET1 (EDN1), complementing the serine carboxypeptidase activity of CathA (CTSA). Analysis with purified recombinant protein confirmed that Scpep1 cleaved the C-terminal trp residue from ET1. || also named MSTP034 AF113214

Relationship

Family : Carboxypeptidase_S10

Block : X

Position in NCBI Life Tree : Homo sapiens

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Eukaryota N E > Opisthokonta N E > Metazoa N E > Eumetazoa N E > Bilateria N E > Deuterostomia N E > Chordata N E > Craniata N E > Vertebrata N E > Gnathostomata N E > Teleostomi N E > Euteleostomi N E > Sarcopterygii N E > Dipnotetrapodomorpha N E > Tetrapoda N E > Amniota N E > Mammalia N E > Theria N E > Eutheria N E > Boreoeutheria N E > Euarchontoglires N E > Primates N E > Haplorrhini N E > Simiiformes N E > Catarrhini N E > Hominoidea N E > Hominidae N E > Homininae N E > Homo N E > Homo sapiens N E

Molecular evidence

No mutation

No structure

No kinetic

No disease

No substrate

Database

Sequence

Peptide

MELALRRSPV PRWLLLLPLL LGLNAGAVID WPTEEGKEVW DYVTVRKDAY MFWWLYYATN SCKNFSELPL VMWLQGGPGG SSTGFGNFEE IGPLDSDLKP RKTTWLQAAS LLFVDNPVGT GFSYVNGSGA YAKDLAMVAS DMMVLLKTFF SCHKEFQTVP FYIFSESYGG KMAAGIGLEL YKAIQRGTIK CNFAGVALGD SWISPVDSVL SWGPYLYSMS LLEDKGLAEV SKVAEQVLNA VNKGLYREAT ELWGKAEMII EQNTDGVNFY NILTKSTPTS TMESSLEFTQ SHLVCLCQRH VRHLQRDALS QLMNGPIRKK LKIIPEDQSW GGQATNVFVN MEEDFMKPVI SIVDELLEAG INVTVYNGQL DLIVDTMGQE AWVRKLKWPE LPKFSQLKWK ALYSDPKSLE TSAFVKSYKN LAFYWILKAG HMVPSDQGDM ALKMMRLVTQ QE

References (8)

Title : Remodeling natural products: chemistry and serine hydrolase activity of a rocaglate-derived beta-lactone - Lajkiewicz_2014_J.Am.Chem.Soc_136_2659
Author(s) : Lajkiewicz NJ , Cognetta AB, 3rd , Niphakis MJ , Cravatt BF , Porco JA, Jr.
Ref : Journal of the American Chemical Society , 136 :2659 , 2014
PubMedID: 24447064
Gene_locus related to this paper: human-ABHD10 , human-ACOT1 , human-ACOT2 , human-CTSA , human-SCPEP1 , mouse-abhda

Title : Serine carboxypeptidase SCPEP1 and Cathepsin A play complementary roles in regulation of vasoconstriction via inactivation of endothelin-1 - Pan_2014_PLoS.Genet_10_e1004146
Author(s) : Pan X , Grigoryeva L , Seyrantepe V , Peng J , Kollmann K , Tremblay J , Lavoie JL , Hinek A , Lubke T , Pshezhetsky AV
Ref : PLoS Genet , 10 :e1004146 , 2014
PubMedID: 24586188
Gene_locus related to this paper: human-SCPEP1 , mouse-RISC

Title : Molecular characterization and gene disruption of mouse lysosomal putative serine carboxypeptidase 1 - Kollmann_2009_Febs.j_276_1356
Author(s) : Kollmann K , Damme M , Deuschl F , Kahle J , D'Hooge R , Lullmann-Rauch R , Lubke T
Ref : Febs J , 276 :1356 , 2009
PubMedID: 19187242
Gene_locus related to this paper: human-SCPEP1 , mouse-RISC

Title : Functional characterization of a putative serine carboxypeptidase in vascular smooth muscle cells - Lee_2009_Circ.Res_105_271
Author(s) : Lee TH , Chen J , Miano JM
Ref : Circulation Research , 105 :271 , 2009
PubMedID: 19574551
Gene_locus related to this paper: human-SCPEP1 , mouse-RISC

Title : Tissue expression of the novel serine carboxypeptidase Scpep1 - Lee_2006_J.Histochem.Cytochem_54_701
Author(s) : Lee TH , Streb JW , Georger MA , Miano JM
Ref : Journal of Histochemistry & Cytochemistry , 54 :701 , 2006
PubMedID: 16461364
Gene_locus related to this paper: human-SCPEP1 , mouse-RISC

Title : Signal peptide prediction based on analysis of experimentally verified cleavage sites - Zhang_2004_Protein.Sci_13_2819
Author(s) : Zhang Z , Henzel WJ
Ref : Protein Science , 13 :2819 , 2004
PubMedID: 15340161
Gene_locus related to this paper: human-PLA2G15 , human-SCPEP1

Title : Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry - Zhang_2003_Nat.Biotechnol_21_660
Author(s) : Zhang H , Li XJ , Martin DB , Aebersold R
Ref : Nat Biotechnol , 21 :660 , 2003
PubMedID: 12754519
Gene_locus related to this paper: human-SCPEP1

Title : Cloning of a novel retinoid-inducible serine carboxypeptidase from vascular smooth muscle cells - Chen_2001_J.Biol.Chem_276_34175
Author(s) : Chen J , Streb JW , Maltby KM , Kitchen CM , Miano JM
Ref : Journal of Biological Chemistry , 276 :34175 , 2001
PubMedID: 11447226
Gene_locus related to this paper: human-SCPEP1 , mouse-RISC , ratno-RISC