rhosm-cocE
Rhodococcus sp. (strain MB1 Bresler) cocaine esterase (EC 3.1.1.-) CoCE
Comment
BZEase1 (V116K\/T172R\/G173Q\/L196C\/I301C)\; BZEase2 (A51L\/V116K\/T172R\/G173Q\/L196C\/I301C)\; BZEase3 (A51R\/V116K\/T172R\/G173Q\/L196C\/I301C)\; BZEase4 (A51K\/V116K\/T172R\/G173Q\/L196C\/I301C)\; 7F65(T172R\/G173Q\/V116K\/S117A\/A51L)\; 9K48 (T172R\/G173Q\/V116K\/S117A\/A51K)\; variant E196-301 (T172R\/G173Q\/L196C\/I301C)
Relationship
Family : Cocaine_esterase
Block : X
Position in NCBI Life Tree : Rhodococcus sp.
(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)
> cellular organisms N E > Bacteria N E > Terrabacteria group N E > Actinobacteria [phylum] N E > Actinobacteria [class] N E > Corynebacteriales N E > Nocardiaceae N E > Rhodococcus N E > Rhodococcus sp. N EMolecular evidence
No mutation
1JU3 - Bacterial (Rhodococcus sp.) Cocaine esterase complex with transition state Analog
1JU4 - Bacterial (Rhodococcus sp.) Cocaine esterase complex with product
1L7Q - Ser117Ala mutant of bacterial cocaine esterase cocE
1L7R - Tyr44Phe mutant of bacterial cocaine esterase cocE
3I2F - Cocaine Esterase with mutations T172R \/ G173Q, bound to DTT adduct (3I2F replaced 2QAW 29-Jun-2009)
3I2G - Cocaine Esterase with mutation G173Q, bound to DTT adduct (3I2G replaced 2QAX 29-Jun-2009)
3I2H - Cocaine Esterase with mutation L169K, bound to DTT adduct (3I2H replaced 2QAV 29-Jun-2009)
3I2I - Cocaine Esterase with mutation T172R, bound to DTT adduct (3I2I replaced 2QAY 29-Jun-2009)
3I2J - Cocaine Esterase, wild type, without a ligand (3I2J replaced 2QAT 29-Jun-2009)
3I2K - Cocaine Esterase, wild type, bound to a DTT adduct (3I2K replaced 2QAU 29-Jun-2009)
3IDA - Cocaine Esterase, with mutations L169K and G173Q, bound to a DTT adduct
3PUH - Cocaine Esterase, wild-type biologically active dimer
3PUI - Cocaine Esterase with mutations G4C, S10C
4P08 - Engineered thermostable dimeric cocaine esterase
7F65 - Bacterial Cocaine Esterase with mutations T172R\/G173Q\/V116K\/S117A\/A51L, bound to benzoic acid
9K48 - Bacterial Cocaine Esterase with mutations T172R\/G173Q\/V116K\/S117A\/A51K (replace 8GYL)
No kinetic
No disease
Sequence
Peptide
MVDGNYSVAS NVMVPMRDGV RLAVDLYRPD ADGPVPVLLV RNPYDKFDVF AWSTQSTNWL EFVRDGYAVV IQDTRGLFAS EGEFVPHVDD EADAEDTLSW ILEQAWCDGN VGMFGVSYLG VTQWQAAVSG VGGLKAIAPS MASADLYRAP WYGPGGALSV EALLGWSALI GTGLITSRSD ARPEDAADFV QLAAILNDVA GAASVTPLAE QPLLGRLIPW VIDQVVDHPD NDESWQSISL FERLGGLATP ALITAGWYDG FVGESLRTFV AVKDNADARL VVGPWSHSNL TGRNADRKFG IAATYPIQEA TTMHKAFFDR HLRGETDALA GVPKVRLFVM GIDEWRDETD WPLPDTAYTP FYLGGSGAAN TSTGGGTLST SISGTESADT YLYDPADPVP SLGGTLLFHN GDNGPADQRP IHDRDDVLCY STEVLTDPVE VTGTVSARLF VSSSAVDTDF TAKLVDVFPD GRAIALCDGI VRMRYRETLV NPTLIEAGEI YEVAIDMLAT SNVFLPGHRI MVQVSSSNFP KYDRNSNTGG VIAREQLEEM CTAVNRIHRG PEHPSHIVLP IIKR
References (15)
| Title : Computationally guided cross-linking overcomes interfacial mismatch in protein dimerization: Creating a long-acting cocaine esterase - Deng_2026_Int.J.Biol.Macromol__153384 |
| Author(s) : Deng X , Wang Z , Hou Y , Xu N , Li Z , Hu Q , Mo N , Ren Z , Chen C , Hou S , Chen X , Ma L |
| Ref : Int J Biol Macromol , :153384 , 2026 |
| Abstract : |
| PubMedSearch : Deng_2026_Int.J.Biol.Macromol__153384 |
| PubMedID: 42409143 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : Substrate Specificity and In Vivo Efficacy of Engineered Cocaine Esterases toward Cocaine and its Toxic Metabolites - Li_2026_Chem.Biol.Interact__112088 |
| Author(s) : Li Z , Hu Q , Deng X , Chen C , Ren Z , Mo N , Huang Z , Wang B , Fang L , Wang J , Hou S , Chen X |
| Ref : Chemico-Biological Interactions , :112088 , 2026 |
| Abstract : |
| PubMedSearch : Li_2026_Chem.Biol.Interact__112088 |
| PubMedID: 42000102 |
| Gene_locus related to this paper: human-BCHE , rhosm-cocE |
| Title : Development of cocaine esterase W\/O\/W nanoemulsions by a novel low-temperature double emulsification approach for cocaine abuse treatment - Wang_2025_Int.J.Biol.Macromol__144642 |
| Author(s) : Wang W , Deng X , Xu N , Li H , Lai S , Li Z , Hu Q , Chi M , Liu F , Ma L , Liu J , Chen W , Hou S , Chen X |
| Ref : Int J Biol Macromol , :144642 , 2025 |
| Abstract : |
| PubMedSearch : Wang_2025_Int.J.Biol.Macromol__144642 |
| PubMedID: 40419064 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : Catalytic mechanism, computational design, and crystal structure of a highly specific and efficient benzoylecgonine hydrolase - Chen_2024_Int.J.Biol.Macromol__137767 |
| Author(s) : Chen X , Zhang Y , Tong J , Ouyang P , Deng X , Zhang J , Liu H , Hu Y , Yao W , Wang J , Wang X , Hou S , Yao J |
| Ref : Int J Biol Macromol , :137767 , 2024 |
| Abstract : |
| PubMedSearch : Chen_2024_Int.J.Biol.Macromol__137767 |
| PubMedID: 39561846 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : Computational Design and Crystal Structure of a Highly Efficient Benzoylecgonine Hydrolase - Chen_2021_Angew.Chem.Int.Ed.Engl_60_21959 |
| Author(s) : Chen X , Deng X , Zhang Y , Wu Y , Yang K , Li Q , Wang J , Yao W , Tong J , Xie T , Hou S , Yao J |
| Ref : Angew Chem Int Ed Engl , 60 :21959 , 2021 |
| Abstract : |
| PubMedSearch : Chen_2021_Angew.Chem.Int.Ed.Engl_60_21959 |
| PubMedID: 34351032 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : Efficient Cocaine Degradation by Cocaine Esterase-Loaded Red Blood Cells - Rossi_2020_Front.Physiol_11_573492 |
| Author(s) : Rossi L , Pierige F , Agostini M , Bigini N , Termopoli V , Cai Y , Zheng F , Zhan CG , Landry DW , Magnani M |
| Ref : Front Physiol , 11 :573492 , 2020 |
| Abstract : |
| PubMedSearch : Rossi_2020_Front.Physiol_11_573492 |
| PubMedID: 33013487 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : Computational design of cephradine synthase in a new scaffold identified from structural databases - Huang_2017_Chem.Commun.(Camb)_53_7604 |
| Author(s) : Huang X , Xue J , Zhu Y |
| Ref : Chem Commun (Camb) , 53 :7604 , 2017 |
| Abstract : |
| PubMedSearch : Huang_2017_Chem.Commun.(Camb)_53_7604 |
| PubMedID: 28639649 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : Rational design, preparation, and characterization of a therapeutic enzyme mutant with improved stability and function for cocaine detoxification - Fang_2014_ACS.Chem.Biol_9_1764 |
| Author(s) : Fang L , Chow KM , Hou S , Xue L , Chen X , Rodgers DW , Zheng F , Zhan CG |
| Ref : ACS Chemical Biology , 9 :1764 , 2014 |
| Abstract : |
| PubMedSearch : Fang_2014_ACS.Chem.Biol_9_1764 |
| PubMedID: 24919140 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : Subunit stabilization and polyethylene glycolation of cocaine esterase improves in vivo residence time - Narasimhan_2011_Mol.Pharmacol_80_1056 |
| Author(s) : Narasimhan D , Collins GT , Nance MR , Nichols J , Edwald E , Chan J , Ko MC , Woods JH , Tesmer JJ , Sunahara RK |
| Ref : Molecular Pharmacology , 80 :1056 , 2011 |
| Abstract : |
| PubMedSearch : Narasimhan_2011_Mol.Pharmacol_80_1056 |
| PubMedID: 21890748 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : The ability of bacterial cocaine esterase to hydrolyze cocaine metabolites and their simultaneous quantification using high-performance liquid chromatography-tandem mass spectrometry - Brim_2011_Mol.Pharmacol_80_1119 |
| Author(s) : Brim RL , Noon KR , Collins GT , Nichols J , Narasimhan D , Sunahara RK , Woods JH |
| Ref : Molecular Pharmacology , 80 :1119 , 2011 |
| Abstract : |
| PubMedSearch : Brim_2011_Mol.Pharmacol_80_1119 |
| PubMedID: 21885621 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : Structural analysis of thermostabilizing mutations of cocaine esterase - Narasimhan_2010_Protein.Eng.Des.Sel_23_537 |
| Author(s) : Narasimhan D , Nance MR , Gao D , Ko MC , MacDonald J , Tamburi P , Yoon D , Landry DM , Woods JH , Zhan CG , Tesmer JJ , Sunahara RK |
| Ref : Protein Engineering Des Sel , 23 :537 , 2010 |
| Abstract : |
| PubMedSearch : Narasimhan_2010_Protein.Eng.Des.Sel_23_537 |
| PubMedID: 20436035 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : A thermally stable form of bacterial cocaine esterase: a potential therapeutic agent for treatment of cocaine abuse - Brim_2010_Mol.Pharmacol_77_593 |
| Author(s) : Brim RL , Nance MR , Youngstrom DW , Narasimhan D , Zhan CG , Tesmer JJ , Sunahara RK , Woods JH |
| Ref : Molecular Pharmacology , 77 :593 , 2010 |
| Abstract : |
| PubMedSearch : Brim_2010_Mol.Pharmacol_77_593 |
| PubMedID: 20086035 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : Biochemical characterization and structural analysis of a highly proficient cocaine esterase - Turner_2002_Biochemistry_41_12297 |
| Author(s) : Turner JM , Larsen NA , Basran A , Barbas CF, 3rd , Bruce NC , Wilson IA , Lerner RA |
| Ref : Biochemistry , 41 :12297 , 2002 |
| Abstract : |
| PubMedSearch : Turner_2002_Biochemistry_41_12297 |
| PubMedID: 12369817 |
| Gene_locus related to this paper: rhosm-cocE |
| Title : Gene cloning and nucleotide sequencing and properties of a cocaine esterase from Rhodococcus sp. strain MB1 - Bresler_2000_Appl.Environ.Microbiol_66_904 |
| Author(s) : Bresler MM , Rosser SJ , Basran A , Bruce NC |
| Ref : Applied Environmental Microbiology , 66 :904 , 2000 |
| Abstract : |
| PubMedSearch : Bresler_2000_Appl.Environ.Microbiol_66_904 |
| PubMedID: 10698749 |
| Gene_locus related to this paper: rhosm-cocE |