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Cocaine_esterase

Relationship

Family: Cocaine_esterase

Block: X

Parent Family: Peptidase_S15

Comment

Contains also alpha-amino acid ester hydrolase (Beta-Lactam Acylase), glutaryl 7-aca acylases, antibiotic hydrolase, diester hydrolase. Able to perform both hydrolysis and synthesis of a variety of alpha-amino beta-lactam antibiotics including (R)-ampicillin and cephalexin. The closest relatives is the S15 X-prolyl amino peptidase of Lactobacillus

Database

Interpro : IPR005674 CocE\/NonD hydrolase

PIRSF : PIRSF031658

Pdoc : No Pdoc

Pfam : PF02129 Peptidase_S15

Prints : No Print

EC Number : 3.1.1.43

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
1JU3 Bacterial (Rhodococcus sp.) Cocaine esterase complex with transition state Analog rhosm-cocE
1JU4 Bacterial (Rhodococcus sp.) Cocaine esterase complex with product rhosm-cocE
1L7Q Ser117Ala mutant of bacterial cocaine esterase cocE rhosm-cocE
1L7R Tyr44Phe mutant of bacterial cocaine esterase cocE rhosm-cocE
1MPX alpha-amino acid ester hydrolase labeled with selenomethionine xanax-GAA
1NX9 Acetobacter turbidans alpha-amino acid ester hydrolase S205A mutant complexed with ampicillin acepa-AEHA
1RYY Acetobacter turbidans alpha-amino acid ester hydrolase Y206A mutant acepa-AEHA
2B4K Acetobacter turbidans alpha-amino acid ester hydrolase complexed with phenylglycine acepa-AEHA
2B9V Acetobacter turbidans alpha-amino acid ester hydrolase acepa-AEHA
3I2F Cocaine Esterase with mutations T172R \/ G173Q, bound to DTT adduct (3I2F replaced 2QAW 29-Jun-2009) rhosm-cocE
3I2G Cocaine Esterase with mutation G173Q, bound to DTT adduct (3I2G replaced 2QAX 29-Jun-2009) rhosm-cocE
3I2H Cocaine Esterase with mutation L169K, bound to DTT adduct (3I2H replaced 2QAV 29-Jun-2009) rhosm-cocE
3I2I Cocaine Esterase with mutation T172R, bound to DTT adduct (3I2I replaced 2QAY 29-Jun-2009) rhosm-cocE
3I2J Cocaine Esterase, wild type, without a ligand (3I2J replaced 2QAT 29-Jun-2009) rhosm-cocE
3I2K Cocaine Esterase, wild type, bound to a DTT adduct (3I2K replaced 2QAU 29-Jun-2009) rhosm-cocE
3IB3 Crystal Structure of SACOL2612 - CocE\/NonD family hydrolase from Staphylococcus aureus staau-SAV2594
3IDA Cocaine Esterase, with mutations L169K and G173Q, bound to a DTT adduct rhosm-cocE
3III 1.95 Angstrom Crystal Structure of CocE\/NonD family hydrolase (SACOL2612) from Staphylococcus aureus staau-SAV2594
3PUH Cocaine Esterase, wild-type biologically active dimer rhosm-cocE
3PUI Cocaine Esterase with mutations G4C, S10C rhosm-cocE
4P08 Engineered thermostable dimeric cocaine esterase rhosm-cocE
4PF1 Crystal structure of aminopeptidase from marine sediment archaeon Thaumarchaeota archaeon 9arch-m7tve7
7F65 Bacterial Cocaine Esterase with mutations T172R\/G173Q\/V116K\/S117A\/A51L, bound to benzoic acid rhosm-cocE
9K48 Bacterial Cocaine Esterase with mutations T172R\/G173Q\/V116K\/S117A\/A51K (replace 8GYL) rhosm-cocE

Gene_locus Name Species
9arch-m7tve7 Thaumarchaeota archaeon SCGC AB-539-E09 MCGE09_00221 Peptidase S15\/CocE\/NonD Thaumarchaeota archaeon SCGC AB-539-E09
9delt-q1nl62 delta proteobacterium MLMS-1 peptidase s15 delta proteobacterium MLMS-1
9delt-q3mvz8 Syntrophobacter fumaroxidans MPOB peptidase s15 Syntrophobacter fumaroxidans MPOB
9flao-q1vns5 Psychroflexus torquis ATCC 700755 x-pro dipeptidyl-peptidase family protein (fragment) Psychroflexus torquis ATCC 700755
9flao-q1vt96 Psychroflexus torquis (strain ATCC 700755 \/ ACAM 623) Acyl esterase, CocE\/NonD family Psychroflexus torquis ATCC 700755
9flao-q1w055 Psychroflexus torquis (strain ATCC 700755 \/ ACAM 623) glutaryl-7-aca acylase Psychroflexus torquis ATCC 700755
9gamm-q33su0 Shewanella sp. PV-4 peptidase s15 precursor Shewanella sp. PV-4
9gamm-q34v04 Alkalilimnicola ehrlichei MLHE-1 hypothetical protein Alkalilimnicola ehrlichei MLHE-1
9gamm-q352d9 Alkalilimnicola ehrlichei MLHE-1 hypothetical protein Alkalilimnicola ehrlichei MLHE-1
9myco-q1tmu7 Mycobacterium sp. KMS MCS and JLS peptidase s15 Mycobacterium sp. JLS
9sphn-q1ng13 Sphingomonas sp. SKA58 putative alpha-amino acid ester hydrolase Sphingomonas sp. SKA58
9sphn-q38ju6 Sphingomonas sp. CFDS-1 esterase Sphingomonas sp. CFDS-1
acepa-AEHA Acetobacter pasteurianus (Acetobacter turbidans) alpha-amino acid ester hydrolase (EC 3.1.1.43) Acetobacter pasteurianus
anavt-q3m571 Anabaena variabilis (strain ATCC 29413 \/ PCC 7937) peptidase s15 Anabaena variabilis
aspfu-q4waa4 Aspergillus fumigatus Af293 hydrolase, coce\/nond family, putative Aspergillus fumigatus Af293
aspor-q2u4f6 Aspergillus oryzae predicted acyl esterases Aspergillus oryzae
aspor-q2u5f5 Aspergillus oryzae Aspergillus flavus predicted acyl esterases Aspergillus oryzae
aspor-q2u854 Aspergillus oryzae predicted acyl esterases Aspergillus oryzae
aspor-q2uc98 Aspergillus oryzae predicted acyl esterases Aspergillus oryzae
aspor-q2ufd8 Aspergillus oryzae predicted acyl esterases Aspergillus oryzae
aspor-q2uhq0 Aspergillus oryzae predicted acyl esterases Aspergillus oryzae
brela-Q45289 Brevibacillus laterosporus glutaryl 7-aca acylase precursor Brevibacillus laterosporus
camje-q29xu7 Campylobacter jejuni hypothetical protein (fragment) Campylobacter jejuni
caucr-CC0940 Caulobacter crescentus acylase, putative Caulobacter crescentus
caucr-CC1622 Caulobacter crescentus antibiotic hydrolase Caulobacter crescentus
chagb-q2hi95 Chaetomium globosum CBS 148.51 hypothetical protein Chaetomium globosum CBS 148.51
chlau-q3e218 Chloroflexus aurantiacus J-10-fl peptidase s15 Chloroflexus aurantiacus J-10-fl
chrsd-q1qv10 Chromohalobacter salexigens (strain DSM 3043 \/ ATCC BAA-138 \/ NCIM 13768) peptidase s15 Chromohalobacter salexigens
chrsd-q1qyj1 Chromohalobacter salexigens (strain DSM 3043 \/ ATCC BAA-138 \/ NCIM 13768) peptidase s15 Chromohalobacter salexigens
chrsd-q1qzd2 Chromohalobacter salexigens (strain DSM 3043 \/ ATCC BAA-138 \/ NCIM 13768) peptidase s15 Chromohalobacter salexigens
colp3-q47yv1 Colwellia psychrerythraea (strain 34H \/ ATCC BAA-681) (Vibrio psychroerythus) putative acylase Colwellia psychrerythraea
crowt-q4c8f7 Crocosphaera watsonii peptidase s15 Crocosphaera watsonii
crypv-q5csw2 Cryptosporidium parvum Cryptosporidium hominis alpha beta hydrolase with 5 transmembrane domains Cryptosporidium parvum
cuppj-q470m2 Cupriavidus pinatubonensis (strain JMP134 \/ LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha) peptidase s15 Ralstonia eutropha
cytjo-q1xqe4 Flavobacterium johnsoniae UW101 peptidase s15 precursor Flavobacterium johnsoniae UW101
ectha-q2cvw3 Halorhodospira halophila SL1 hypothetical protein Halorhodospira halophila SL1
emeni-q5ata4 Emericella nidulans (Aspergillus nidulans) hypothetical protein Aspergillus nidulans FGSC A4
emeni-q5ay87 Aspergillus nidulans FGSC A4 hypothetical protein Aspergillus nidulans FGSC A4
flabb-q26gp6 Flavobacteria bacterium (strain BBFL7) peptidase, s15 family Flavobacteria bacterium BBFL7
frasc-q2j561 Frankia sp. (strain CcI3) peptidase s15 Frankia sp. CcI3
frasn-A8L6Z2 Frankia sp. EAN1pec peptidase s15 precursor Frankia sp. EAN1pec
frasn-A8LEG4 Frankia sp. EAN1pec peptidase s15 Frankia sp. EAN1pec
frasn-a8l265 Frankia sp. (strain EAN1pec) peptidase s15 Frankia sp. EAN1pec
frasn-a8leg6 Frankia sp. (strain EAN1pec) peptidase s15 Frankia sp. EAN1pec
frasn-a8lfl1 Frankia sp. (strain EAN1pec) peptidase s15 Frankia sp. EAN1pec
gibze-q4ict8 Gibberella zeae (Fusarium graminearum) PepX_C domain-containing protein Gibberella zeae
gibze-q4icv0 Gibberella zeae (Fusarium graminearum) PepX_C domain-containing protein Gibberella zeae
gibze-q4idr7 Gibberella zeae (Fusarium graminearum) PepX_C domain-containing protein Gibberella zeae
gibze-q4igc8 Gibberella zeae (Fusarium graminearum) PepX_C domain-containing protein Gibberella zeae
gluox-q5fn25 Gluconobacter oxydans (Gluconobacter suboxydans) alpha-amino acid ester hydrolase (EC 3.1.1.43) Gluconobacter oxydans
halhl-a1wxe8 Halorhodospira halophila (strain DSM 244 \/ SL1) (Ectothiorhodospira halophila (strain DSM 244 \/ SL1)) hypothetical protein Halorhodospira halophila SL1
jansc-q28kn1 Jannaschia sp. (strain CCS1) peptidase s15 Jannaschia sp. CCS1
lacla-YMGC Lactococcus lactis (subsp. lactis) (Streptococcus lactis) hypothetical protein ymgc Lactococcus lactis
leima-q4q871 Leishmania major putative Leishmania major
lisin-LIN2898 Listeria innocua hypothetical protein lin2898 Listeria innocua
lismo-LMO0493 Listeria monocytogenes, Listeria seeligeri, hypothetical protein lmo0493 Listeria monocytogenes
lismo-LMO2755 Listeria monocytogenes, Hydrolase, CocE\/NonD family hypothetical protein lmo2755 Listeria monocytogenes
maggr-q2kg35 Magnaporthe grisea 70-15 hypothetical protein Magnaporthe grisea 70-15
meslo-MLL5128 Rhizobium loti (Mesorhizobium loti) hypothetical protein mll5128 Rhizobium loti
meslo-mlr6999 Mesorhizobium loti (Rhizobium loti) mlr6999 c-term similar to ab-hydrolase Mesorhizobium loti
mobas-q1ylc5 Aurantimonas manganoxydans SI85-9A1 putative hydrolase, coce\/nond family Aurantimonas manganoxydans SI85-9A1
mycgi-a4tcg0 Mycobacterium gilvum (Mycobacterium flavescens), Mycobacterium sp., peptidase s15 Mycobacterium gilvum PYR-GCK
mycpa-q73vv9 Mycobacterium paratuberculosis hypothetical protein Mycobacterium paratuberculosis
mycpa-q73wu7 Mycobacterium paratuberculosis hypothetical protein Mycobacterium paratuberculosis
mycss-q1ba85 Mycobacterium sp. KMS JLS and MCS peptidase s15 Mycobacterium sp. JLS
myctu-RV1215C Mycobacterium tuberculosis, Mycobacterium bovis hypothetical 62.6 kda protein (diester hydrolase, putative) RV1215C Mycobacterium tuberculosis
myctu-RV2800 Mycobacterium tuberculosis, Mycobacterium bovis hypothetical 60.3 kda protein (diester hydrolase, putative) RV2800 Mycobacterium tuberculosis
myctu-y1835 Mycobacterium tuberculosis, Mycobacterium bovis hypothetical protein Rv1835c\/MT1883 Mycobacterium tuberculosis
mycvn-q25uy0 Mycobacterium vanbaalenii PYR-1 peptidase s15 Mycobacterium vanbaalenii PYR-1
neucr-B11O8.160 Neurospora crassa hypothetical protein b11o8.160 Neurospora crassa
nitoc-q3j915 Nitrosococcus oceani (strain ATCC 19707 \/ NCIMB 11848) peptidase s15 Nitrosococcus oceani
nocfa-q5yp51 Nocardia farcinica putative peptidase Nocardia farcinica
nocfa-q5yrp2 Nocardia farcinica putative esterase Nocardia farcinica
nocfa-q5ytz8 Nocardia farcinica hypothetical protein Nocardia farcinica
nocsj-A1SDS2 Nocardioides sp. JS614 peptidase s15 precursor Nocardioides sp. JS614
nocsj-A1SII2 Nocardioides sp. JS614 peptidase s15 precursor Nocardioides sp. JS614
paruw-q6mez2 Parachlamydia sp. (strain UWE25) (subsp. Acanthamoeba sp.) putative acylase and diesterase Candidatus Protochlamydia amoebophila UWE25
prom9-q31bu9 Prochlorococcus marinus (strain MIT 9312) hypothetical protein Prochlorococcus marinus
proma-q7vbl5 Prochlorococcus marinus alpha\/beta superfamily hydrolase Prochlorococcus marinus
promm-q7v8b7 Prochlorococcus marinus (strain MIT 9313) hypothetical protein precursor Prochlorococcus marinus
promp-q7v298 Prochlorococcus marinus subsp. pastoris (strain CCMP 1378 \/ MED4) hypothetical protein Prochlorococcus marinus subsp. pastoris
promt-q46lw5 Prochlorococcus marinus (strain NATL2A) alpha\/beta superfamily hydrolase Prochlorococcus marinus
psea6-q15xd2 Pseudoalteromonas atlantica (strain T6c \/ ATCC BAA-1087) peptidase s15 precursor Pseudoalteromonas atlantica T6c
rhiec-q2k1i0 Rhizobium etli (strain CFN 42 \/ ATCC 51251) hypothetical conserved protein Rhizobium etli
rhil3-q1m7c3 Rhizobium leguminosarum bv. viciae (strain 3841) putative peptidase Rhizobium leguminosarum bv. viciae
rhime-R02260 Rhizobium meliloti (Sinorhizobium meliloti) Rhizobium sp., Sinorhizobium medicae, hypothetical protein r02260 Sinorhizobium meliloti
rhime-RB1252 Rhizobium meliloti (Sinorhizobium meliloti) putative esterase or acylase protein Sinorhizobium meliloti
rhop2-q2ive8 Rhodopseudomonas palustris (strain HaA2) peptidase s15 Rhodopseudomonas palustris
rhos4-q3ixt3 Rhodobacter sphaeroides (strain ATCC 17023 \/ 2.4.1 \/ NCIB 8253 \/ DS158) hypothetical protein Rhodobacter sphaeroides
rhosm-cocE Rhodococcus sp. (strain MB1 Bresler) cocaine esterase (EC 3.1.1.-) Rhodococcus sp.
rubxd-Q1ATG7 Rubrobacter xylanophilus DSM 9941 peptidase s15 Rubrobacter xylanophilus DSM 9941
rubxd-Q1ATJ1 Rubrobacter xylanophilus (strain DSM 9941 \/ NBRC 16129) peptidase s15 Rubrobacter xylanophilus DSM 9941
shefr-q3nqe4 Shewanella frigidimarina NCIMB 400 peptidase s15 Shewanella frigidimarina NCIMB 400
silpo-q5lp56 Silicibacter pomeroyi x-pro dipeptidyl-peptidase family protein Silicibacter pomeroyi
silst-q1gjp2 Silicibacter sp. (strain TM1040) peptidase s15 Silicibacter sp. TM1040
solue-q026r6 Solibacter usitatus Ellin6076 peptidase s15 precursor Solibacter usitatus Ellin6076
solus-q43v49 Solibacter usitatus Ellin6076 peptidase s15 precursor Solibacter usitatus Ellin6076
solus-q443k2 Solibacter usitatus Ellin6076 peptidase s15 precursor Solibacter usitatus Ellin6076
solus-q443u7 Solibacter usitatus Ellin6076 peptidase s15 Solibacter usitatus Ellin6076
staau-SAV2594 Staphylococcus aureus COCE\/NOND family hydrolase, S15 family peptidase Staphylococcus aureus
stacp-b9cph7 Staphylococcus capitis, Staphylococcus caprae Hydrolase, CocE\/NonD family Staphylococcus capitis SK14
strav-SAV4863 Streptomyces avermitilis hypothetical protein Streptomyces avermitilis
strgr-NOND Streptomyces griseus subsp. griseus putative diester hydrolase Streptomyces griseus subsp. griseus
strro-STTE Streptomyces rochei (Streptomyces parvullus) putative acylase Streptomyces rochei
synja-q2jqp2 Synechococcus sp. (strain JA-3-3Ab) (Cyanobacteria bacterium Yellowstone A-Prime) (strain JA-2-3B'a(2-13))(B-Prime) x-pro dipeptidyl-peptidase (s15 family) Synechococcus sp.
synp6-q5n3k1 Synechococcus sp. (strain PCC 6301) (Anacystis nidulans) (strain PCC 7942) (Anacystis nidulans R2) hypothetical protein Synechococcus sp.
synpx-q7u648 Synechococcus sp. (strain WH8102) predicted acyl esterase (cog2936) Synechococcus sp.
syns9-q3aye0 Synechococcus sp. (strain CC9902) acyl esterase Synechococcus sp.
synsc-q3akv4 Synechococcus sp. (strain CC9605) acyl esterase precursor Synechococcus sp.
synsp-SLR1771 Synechocystis sp. (strain PCC 6803) hypothetical protein slr1771 Synechocystis sp.
trier-q3hes5 Trichodesmium erythraeum IMS101 peptidase s15 Trichodesmium erythraeum IMS101
trycr-q4de42 Trypanosoma cruzi dipeptidyl-peptidase, putative Trypanosoma cruzi
ustma-q4p906 Ustilago maydis (strain 521 \/ FGSC 9021) (Corn smut fungus) hypothetical protein Ustilago maydis 521
xanax-GAA Xanthomonas axonopodis, Xanthomonas campestris (Xanthomonas citri), Xanthomonas oryzae, Xanthomonas fuscans, Xanthomonas gardneri,Xanthomonas perforans, glutaryl-7-aca acylase Xanthomonas axonopodis
xylfa-XF1029 Xylella fastidiosa glutaryl-7-aca acylase precursor Xylella fastidiosa
zymmo-GAA Zymomonas mobilis glutaryl-7-aca acylase Zymomonas mobilis

Gene_locus_frgt Name Species
aspfn-B8N2U3 Aspergillus oryzae, Aspergillus flavus Predicted acyl esterase Aspergillus oryzae
emeni-q5av91 Emericella nidulans (Aspergillus nidulans) Putative uncharacterized protein Aspergillus nidulans FGSC A4
mycvn-q25u34 Mycobacterium vanbaalenii PYR-1 hypothetical protein Mycobacterium vanbaalenii PYR-1
nocsj-A1SDE1 Nocardioides sp. JS614 similar to acyl esterases precursor Nocardioides sp. JS614

Substrate Chemical Nomenclature Proteins
6-aminopenicillinate (2S,5R,6R)-6-amino-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid xanax-GAA    acepa-AEHA
Ampicillin (2S,5R,6R)-6-[[(2R)-2-amino-2-phenylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid xanax-GAA    acepa-AEHA    pig-EST1    pig-a0a1s6l967
Benzoylecgonine (1S,3S,4R,5R)-3-benzoyloxy-8-methyl-8-azabicyclo[3.2.1]octane-4-carboxylic acid human-BCHE    rhosm-cocE
Cocaine methyl (1S,3S,4R,5R)-3-benzoyloxy-8-methyl-8-azabicyclo[3.2.1]octane-4-carboxylate human-BCHE    human-CES1
D-PGME methyl (2R)-2-amino-2-phenylacetate xanax-GAA    acepa-AEHA
D-Phenylglycine (2R)-2-amino-2-phenylacetic acid acepa-AEHA    xanax-GAA
Glutaryl-7-aca (6R,7R)-3-(acetyloxymethyl)-7-(4-carboxybutanoylamino)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid brela-Q45289

Inhibitor Chemical Nomenclature Proteins
Benzoic-acid benzoic acid sorbi-hnl    strau-cpoT    psefl-cumD    rhosm-cocE    rhoso-halo1    idesa-mheth    hevbr-hnl
DFP Diisopropylphosphorofluoridate arcfu-AF1763    human-DPP4    human-ACHE    human-BCHE    human-PLA2G7    bacce-BC3734    ecoli-IROE    fusso-cutas    human-FAP    human-CTSA    aspni-FAEA
DTT-adduct (4S,5S)-4,5-bis(sulfanylmethyl)-1,3-dioxolane-2,2-diol rhosm-cocE
PMSF Phenylmethylsulfonyl fluoride theet-q3ch51    emeni-q5avy9    human-ABHD10    human-PREPL    myctu-Rv1430    psefl-cxest    9bact-E40    thefu-q6a0i4    ecoli-Aes    orysj-Q10QA5    bac25-mglp    9bact-Q0GMU2    bacsu-FENB    psepu-clcd1    thema-TM0077    bacsu-RsbQ    ecoli-bioh    drome-CG9542    human-FAP    tubmm-TmEst2    human-NOTUM    cutac-e6dae5    9gamm-urc15994    9baci-EstD9    geost-est30    pholl-AntI
Phenylboronic-acid phenylboronic acid rhosm-cocE

References (3)

Title : Amino ester hydrolase from Xanthomonas campestris pv. campestris, ATCC 33913 for enzymatic synthesis of ampicillin - Blum_2010_J.Mol.Catal.B.Enzym_67_21
Author(s) : Blum JK , Bommarius AS
Ref : J Mol Catal B Enzym , 67 :21 , 2010
Abstract :
PubMedSearch : Blum_2010_J.Mol.Catal.B.Enzym_67_21
PubMedID: 22087071
Gene_locus related to this paper: xanax-GAA

Title : Crystal structure of a bacterial cocaine esterase -
Author(s) : Larsen NA , Turner JM , Stevens J , Rosser SJ , Basran A , Lerner RA , Bruce NC , Wilson IA
Ref : Nat Struct Biol , 9 :17 , 2002
PubMedID: 11742345
Gene_locus related to this paper: rhosm-cocE

Title : Gene cloning and nucleotide sequencing and properties of a cocaine esterase from Rhodococcus sp. strain MB1 - Bresler_2000_Appl.Environ.Microbiol_66_904
Author(s) : Bresler MM , Rosser SJ , Basran A , Bruce NC
Ref : Applied Environmental Microbiology , 66 :904 , 2000
Abstract :
PubMedSearch : Bresler_2000_Appl.Environ.Microbiol_66_904
PubMedID: 10698749
Gene_locus related to this paper: rhosm-cocE